Restriction map for
pFN21AE9256
(Circular) (Six-base) MAPSORT of: pFN21AE9256.seq Check: 7432 from: 1 to: 6859
pFN21AE9256 6859 bp
With 182 enzymes: SgfI *
July 6, 2012 17:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2817 3814 4092 2817
Size: 997 278 5584
Fragments arranged by size:
5584 997 278
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4669 278
Size: 53 83 186 936 3133 2468
Fragments arranged by size:
3133 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2304 4202 1937
Size: 367 1898 4594
Fragments arranged by size:
4594 1898 367
Acc65I G'GTAC_C
Cuts at: 4180 4180
Size: 6859
AclI AA'CG_TT
Cuts at: 1563 4986 5359 1563
Size: 3423 373 3063
Fragments arranged by size:
3423 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2327 2510 2801 3455 3464 3836 3863 4123
Size: 695 183 291 654 9 372 27 260
Cuts at: 4123 4922 6306 6574 1632
Size: 799 1384 268 1917
Fragments arranged by size:
1917 1384 799 695 654 372 291 268
260 183 27 9
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6662
AflIII A'CryG_T
Cuts at: 1203 5758 6735 1203
Size: 4555 977 1327
Fragments arranged by size:
4555 1327 977
AgeI A'CCGG_T
Cuts at: 5690 5690
Size: 6859
AhdI GACnn_n'nnGTC
Cuts at: 5588 5588
Size: 6859
AleI CACnn'nnGTG
Cuts at: 1166 3656 1166
Size: 2490 4369
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2453 1184
Size: 1269 5590
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3459 3491 4031 4063 3459
Size: 32 540 32 6255
Fragments arranged by size:
6255 540 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2572 2698 3109 3382 3673 4036 4602 6174 2572
Size: 126 411 273 291 363 566 1572 3257
Fragments arranged by size:
3257 1572 566 411 363 291 273 126
ApaLI G'TGCA_C
Cuts at: 3338 4079 4916 6072 3338
Size: 741 837 1156 4125
Fragments arranged by size:
4125 1156 837 741
ApoI r'AATT_y
Cuts at: 1493 4168 4394 4546 6655 1493
Size: 2675 226 152 2109 1697
Fragments arranged by size:
2675 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5413 160
Size: 872 4381 1606
Fragments arranged by size:
4381 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6859
AvaI C'yCGr_G
Cuts at: 1870 1943 2247 3081 4015 4130 4184 1870
Size: 73 304 834 934 115 54 4545
Fragments arranged by size:
4545 934 834 304 115 73 54
AvrII C'CTAG_G
Cuts at: 2762 2762
Size: 6859
BamHI G'GATC_C
Cuts at: 4189 4556 4189
Size: 367 6492
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2877 3749 4089 4180 5635
Size: 325 217 627 1090 872 340 91 1455
Cuts at: 5635 618
Size: 1842
Fragments arranged by size:
1842 1455 1090 872 627 340 325 217
91
BanII G_rGCy'C
Cuts at: 1391 2248 2322 3123 3595 3726 4071 4137 4178
Size: 857 74 801 472 131 345 66 41
Cuts at: 4178 1391
Size: 4072
Fragments arranged by size:
4072 857 801 472 345 131 74 66
41
BbeI G_GCGC'C
Cuts at: 2881 4093 2881
Size: 1212 5647
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2016 3006 928
Size: 971 117 990 4781
Fragments arranged by size:
4781 990 971 117
BbvCI CC'TCA_GC
Cuts at: 3318 4115 3318
Size: 797 6062
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5050 5084 1903
Size: 34 3113 34 3678
Fragments arranged by size:
3678 3113 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4751 5961 4751
Size: 1210 5649
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6859
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5470 136
Size: 107 122 71 631 605 3798 1525
Fragments arranged by size:
3798 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2048 6854 2048
Size: 4806 2053
BlpI GC'TnA_GC
Cuts at: 3366 4276 3366
Size: 910 5949
Bme1580I G_kGCm'C
Cuts at: 1790 3342 4083 4920 6076 1790
Size: 1552 741 837 1156 2573
Fragments arranged by size:
2573 1552 1156 837 741
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6859
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4590 5548 442
Size: 416 53 83 399 24 3173 958 1753
Fragments arranged by size:
3173 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6859
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2104 2104
Size: 6859
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2177 2279 2408 2573 2621 2708 2825 2939
Size: 938 102 129 165 48 87 117 114
Cuts at: 2939 2979 3110 3179 3404 3443 3469 3569 3590
Size: 40 131 69 225 39 26 100 21
Cuts at: 3590 3602 4058 5519 1239
Size: 12 456 1461 2579
Fragments arranged by size:
2579 1461 938 456 225 165 131 129
117 114 102 100 87 69 48 40
39 26 21 12
Bpu10I CC'TnA_GC
Cuts at: 2981 3318 3506 4115 2981
Size: 337 188 609 5725
Fragments arranged by size:
5725 609 337 188
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3788 3842 4984 5849 6147 3788
Size: 54 1142 865 298 4500
Fragments arranged by size:
4500 1142 865 298 54
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2468 2819 3161 5522 882
Size: 617 969 351 342 2361 2219
Fragments arranged by size:
2361 2219 969 617 351 342
BsaAI yAC'GTr
Cuts at: 493 1204 4650 493
Size: 711 3446 2702
Fragments arranged by size:
3446 2702 711
BsaBI GATnn'nnATC
Cuts at: 3929 6853 3929
Size: 2924 3935
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2878 4090 4666 5048
Size: 53 83 186 936 1345 1212 576 382
Cuts at: 5048 5683 275
Size: 635 1451
Fragments arranged by size:
1451 1345 1212 936 635 576 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5292 5690 5964 6111 6522 5292
Size: 398 274 147 411 5629
Fragments arranged by size:
5629 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3738 3768 3932 3962 4031
Size: 30 165 30 2335 30 164 30 69
Cuts at: 4031 4061 1178
Size: 30 3976
Fragments arranged by size:
3976 2335 165 164 69 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2381 2810 3131 3164 3401 3506 3536 3566
Size: 1195 429 321 33 237 105 30 30
Cuts at: 3566 3759 1186
Size: 193 4286
Fragments arranged by size:
4286 1195 429 321 237 193 105 33
30 30
BseYI C'CCAG_C
Cuts at: 2872 2952 3291 3391 3757 6062 6558 2872
Size: 80 339 100 366 2305 496 3173
Fragments arranged by size:
3173 2305 496 366 339 100 80
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3350 3617 3659 1163
Size: 573 1614 267 42 4363
Fragments arranged by size:
4363 1614 573 267 42
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2308 3279 4309 5070 6098 1540
Size: 275 186 307 971 1030 761 1028 2301
Fragments arranged by size:
2301 1030 1028 971 761 307 275 186
BsiHKAI G_wGCw'C
Cuts at: 2322 3123 3342 3595 3978 4071 4083 4137 4920
Size: 801 219 253 383 93 12 54 783
Cuts at: 4920 5005 6076 2322
Size: 85 1071 3105
Fragments arranged by size:
3105 1071 801 783 383 253 219 93
85 54 12
BsmI GAATG_Cn'
Cuts at: 4370 4463 4370
Size: 93 6766
BsmBI CGTCTCn'nnnn_
Cuts at: 2891 2891
Size: 6859
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2248 2322 3123 3342 3595 3726 3978
Size: 399 458 74 801 219 253 131 252
Cuts at: 3978 4071 4083 4137 4178 4920 5005 6076 1391
Size: 93 12 54 41 742 85 1071 2174
Fragments arranged by size:
2174 1071 801 742 458 399 253 252
219 131 93 85 74 54 41 12
BspEI T'CCGG_A
Cuts at: 6522 6522
Size: 6859
BspHI T'CATG_A
Cuts at: 4748 6478 4748
Size: 1730 5129
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2598 2817 3118 3814 4092 4214 844
Size: 1754 219 301 696 278 122 3489
Fragments arranged by size:
3489 1754 696 301 278 219 122
BsrBI CCG'CTC
Cuts at: 1394 1823 2209 2350 3226 3538 3586 4746 1394
Size: 429 386 141 876 312 48 1160 3507
Fragments arranged by size:
3507 1160 876 429 386 312 141 48
BsrDI GCAATG_nn'
Cuts at: 65 1248 5354 5528 65
Size: 1183 4106 174 1396
Fragments arranged by size:
4106 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3914 3951 5503 5690 1700
Size: 110 2104 37 1552 187 2869
Fragments arranged by size:
2869 2104 1552 187 110 37
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6859
BssHII G'CGCG_C
Cuts at: 1925 2802 3730 1925
Size: 877 928 5054
Fragments arranged by size:
5054 928 877
BssSI C'ACGA_G
Cuts at: 3173 3677 4919 5931 3173
Size: 504 1242 1012 4101
Fragments arranged by size:
4101 1242 1012 504
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2149 2572 3109 3337 3673 3900 794
Size: 1355 423 537 228 336 227 3753
Fragments arranged by size:
3753 1355 537 423 336 228 227
BstBI TT'CG_AA
Cuts at: 2183 5663 2183
Size: 3480 3379
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6859
BstXI CCAn_nnnn'nTGG
Cuts at: 3016 3658 3805 3016
Size: 642 147 6070
Fragments arranged by size:
6070 642 147
BstYI r'GATC_y
Cuts at: 1972 2048 2175 2499 2683 3879 4189 4556 4941
Size: 76 127 324 184 1196 310 367 385
Cuts at: 4941 4958 6410 6496 6508 6854 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1196 385 367 346 324 310
184 127 86 76 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2028 2973 3735 3936 4059 4152
Size: 552 939 24 945 762 201 123 93
Cuts at: 4152 513
Size: 3220
Fragments arranged by size:
3220 945 939 762 552 201 123 93
24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6120
Fragments arranged by size:
6120 683 56
BtsI GCAGTG_nn'
Cuts at: 2408 2519 4375 5169 5189 2408
Size: 111 1856 794 20 4078
Fragments arranged by size:
4078 1856 794 111 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6859
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6824
DraI TTT'AAA
Cuts at: 4163 4163
Size: 6859
DraIII CAC_nnn'GTG
Cuts at: 4060 4060
Size: 6859
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5866 809
Size: 507 4550 1802
Fragments arranged by size:
4550 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2937 3136 3276
Size: 54 1425 50 275 18 1107 199 140
Cuts at: 3276 3660 4306 5195 6824 8
Size: 384 646 889 1629 43
Fragments arranged by size:
1629 1425 1107 889 646 384 275 199
140 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3276 4306 1537
Size: 275 1464 1030 4090
Fragments arranged by size:
4090 1464 1030 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2841 4142 4789 1353
Size: 1488 1301 647 3423
Fragments arranged by size:
3423 1488 1301 647
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2378 3512 3716 5446 5820 5966
Size: 1064 138 789 1134 204 1730 374 146
Cuts at: 5966 387
Size: 1280
Fragments arranged by size:
1730 1280 1134 1064 789 374 204 146
138
EcoICRI GAG'CTC
Cuts at: 2320 3121 3593 4069 4135 2320
Size: 801 472 476 66 5044
Fragments arranged by size:
5044 801 476 472 66
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2177 2279 2327 2408 2510 2573 2621
Size: 393 545 102 48 81 102 63 48
Cuts at: 2621 2708 2801 2825 2939 2979 3110 3179 3404
Size: 87 93 24 114 40 131 69 225
Cuts at: 3404 3443 3455 3464 3469 3569 3590 3602 3836
Size: 39 12 9 5 100 21 12 234
Cuts at: 3836 3863 4058 4123 4922 5519 6306 6574 1239
Size: 27 195 65 799 597 787 268 1524
Fragments arranged by size:
1524 799 787 597 545 393 268 234
225 195 131 114 102 102 100 93
87 81 69 65 63 48 48 40
39 27 24 21 12 12 9 5
EcoNI CCTnn'n_nnAGG
Cuts at: 2198 3677 2198
Size: 1479 5380
EcoO109I rG'GnC_Cy
Cuts at: 3205 3252 3379 3205
Size: 47 127 6685
Fragments arranged by size:
6685 127 47
EcoRI G'AATT_C
Cuts at: 4168 4546 4168
Size: 378 6481
FspI TGC'GCA
Cuts at: 5365 5365
Size: 6859
HaeII r_GCGC'y
Cuts at: 1678 2551 2881 3066 3081 3182 3354 3673 4093
Size: 873 330 185 15 101 172 319 420
Cuts at: 4093 6006 1678
Size: 1913 2531
Fragments arranged by size:
2531 1913 873 420 330 319 185 172
101 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2668 2700 3349 3381 3738
Size: 32 135 32 1096 32 649 32 357
Cuts at: 3738 3770 5500 5532 5574 5606 1373
Size: 32 1730 32 42 32 2626
Fragments arranged by size:
2626 1730 1096 649 357 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2305 4203 4449 669
Size: 988 281 367 1898 246 3079
Fragments arranged by size:
3079 1898 988 367 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6859
HpaI GTT'AAC
Cuts at: 4449 4449
Size: 6859
KasI G'GCGC_C
Cuts at: 2877 4089 2877
Size: 1212 5647
KpnI G_GTAC'C
Cuts at: 4184 4184
Size: 6859
MfeI C'AATT_G
Cuts at: 4458 4458
Size: 6859
MluI A'CGCG_T
Cuts at: 6735 6735
Size: 6859
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2538 3126 5973 6157 1305
Size: 1233 588 2847 184 2007
Fragments arranged by size:
2847 2007 1233 588 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2939 3662 10
Size: 54 1425 343 1107 723 3207
Fragments arranged by size:
3207 1425 1107 723 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2201 3656 4076 4817 5176 5335 518
Size: 648 1035 1455 420 741 359 159 2042
Fragments arranged by size:
2042 1455 1035 741 648 420 359 159
MspA1I CmG'CkG
Cuts at: 1606 2155 2386 2584 2788 3109 3135 3142 3295
Size: 549 231 198 204 321 26 7 153
Cuts at: 3295 3370 3580 3694 3853 4275 4952 6100 6345
Size: 75 210 114 159 422 677 1148 245
Cuts at: 6345 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 549 422 321 245 231
210 204 198 159 153 114 75 26
7
NarI GG'CG_CC
Cuts at: 2878 4090 2878
Size: 1212 5647
NcoI C'CATG_G
Cuts at: 513 1065 2004 2028 2973 3735 513
Size: 552 939 24 945 762 3637
Fragments arranged by size:
3637 945 939 762 552 24
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6859
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6859
NotI GC'GGCC_GC
Cuts at: 4306 4306
Size: 6859
NspI r_CATG'y
Cuts at: 1132 1229 2152 3310 3421 4217 5762 1132
Size: 97 923 1158 111 796 1545 2229
Fragments arranged by size:
2229 1545 1158 923 796 111 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6859
PciI A'CATG_T
Cuts at: 5758 5758
Size: 6859
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2974 3145 4153 1108
Size: 1866 171 1008 3814
Fragments arranged by size:
3814 1866 1008 171
PfoI T'CCnGG_A
Cuts at: 2628 2841 3882 2628
Size: 213 1041 5605
Fragments arranged by size:
5605 1041 213
PmeI GTTT'AAAC
Cuts at: 4163 4163
Size: 6859
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4031 4063 4928 4960 6441 6473 4031
Size: 32 865 32 1481 32 4417
Fragments arranged by size:
4417 1481 865 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3205 3252 3205
Size: 47 6812
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6859
PsiI TTA'TAA
Cuts at: 4429 4429
Size: 6859
PspXI vC'TCGA_Gb
Cuts at: 2247 4130 2247
Size: 1883 4976
PstI C_TGCA'G
Cuts at: 830 2130 2514 2577 2865 3030 3114 3600 3699
Size: 1300 384 63 288 165 84 486 99
Cuts at: 3699 4211 830
Size: 512 3478
Fragments arranged by size:
3478 1300 512 486 384 288 165 99
84 63
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6859
PvuII CAG'CTG
Cuts at: 2155 2386 2584 2788 3109 3142 3295 3370 3580
Size: 231 198 204 321 33 153 75 210
Cuts at: 3580 3694 2155
Size: 114 5320
Fragments arranged by size:
5320 321 231 210 204 198 153 114
75 33
SacI G_AGCT'C
Cuts at: 2322 3123 3595 4071 4137 2322
Size: 801 472 476 66 5044
Fragments arranged by size:
5044 801 476 472 66
SalI G'TCGA_C
Cuts at: 1936 2303 4201 1936
Size: 367 1898 4594
Fragments arranged by size:
4594 1898 367
SapI GCTCTTCn'nnn_
Cuts at: 2841 4142 2841
Size: 1301 5558
SbfI CC_TGCA'GG
Cuts at: 4211 4211
Size: 6859
ScaI AGT'ACT
Cuts at: 3284 5107 6819 3284
Size: 1823 1712 3324
Fragments arranged by size:
3324 1823 1712
SfcI C'TryA_G
Cuts at: 826 1045 2126 2510 2573 2861 3026 3110 3596
Size: 219 1081 384 63 288 165 84 486
Cuts at: 3596 3695 4207 5342 6023 6214 826
Size: 99 512 1135 681 191 1471
Fragments arranged by size:
1471 1135 1081 681 512 486 384 288
219 191 165 99 84 63
SfoI GGC'GCC
Cuts at: 2879 4091 2879
Size: 1212 5647
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6859
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6859
SmaI CCC'GGG
Cuts at: 1872 3083 4186 1872
Size: 1211 1103 4545
Fragments arranged by size:
4545 1211 1103
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2247 3767 3857 4130 4963 5864
Size: 197 926 304 1520 90 273 833 901
Cuts at: 5864 6126 820
Size: 262 1553
Fragments arranged by size:
1553 1520 926 901 833 304 273 262
197 90
SnaBI TAC'GTA
Cuts at: 493 4650 493
Size: 4157 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6859
SphI G_CATG'C
Cuts at: 1132 2152 3310 4217 1132
Size: 1020 1158 907 3774
Fragments arranged by size:
3774 1158 1020 907
SspI AAT'ATT
Cuts at: 5 52 4783 5
Size: 47 4731 2081
Fragments arranged by size:
4731 2081 47
StuI AGG'CCT
Cuts at: 1517 3899 1517
Size: 2382 4477
StyI C'CwwG_G
Cuts at: 513 1065 2004 2028 2762 2973 3219 3735 3798
Size: 552 939 24 734 211 246 516 63
Cuts at: 3798 4298 513
Size: 500 3074
Fragments arranged by size:
3074 939 734 552 516 500 246 211
63 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3993 4886 4903 5056 5241 5686 325
Size: 1255 2413 893 17 153 185 445 1498
Fragments arranged by size:
2413 1498 1255 893 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3282 5105 6817
Size: 274 80 33 51 1443 1305 1823 1712
Cuts at: 6817 96
Size: 138
Fragments arranged by size:
1823 1712 1443 1305 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2498 2530 6819 6851 2498
Size: 32 4289 32 2506
Fragments arranged by size:
4289 2506 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6859
XbaI T'CTAG_A
Cuts at: 4195 4195
Size: 6859
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 6859
XhoI C'TCGA_G
Cuts at: 1943 2247 4130 1943
Size: 304 1883 4672
Fragments arranged by size:
4672 1883 304
XmaI C'CCGG_G
Cuts at: 1870 3081 4184 1870
Size: 1211 1103 4545
Fragments arranged by size:
4545 1211 1103
XmnI GAAnn'nnTTC
Cuts at: 2060 4988 2060
Size: 2928 3931
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4667 276
Size: 53 83 186 936 3133 2468
Fragments arranged by size:
3133 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspXI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI ApaI AscI BaeI BfrBI BsiWI BstZ17I Bsu36I
EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PmlI PspOMI PsrI RsrII SacII SanDI SexAI
SfiI SrfI SwaI