Restriction map for
pFN21AE9251
(Circular) (Six-base) MAPSORT of: pFN21AE9251.seq Check: 5251 from: 1 to: 6388
pFN21AE9251 6388 bp
With 182 enzymes: SgfI *
August 1, 2019 17:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3627 3627
Size: 6388
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4198 278
Size: 53 83 186 936 2662 2468
Fragments arranged by size:
2662 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2655 3731 1937
Size: 718 1076 4594
Fragments arranged by size:
4594 1076 718
Acc65I G'GTAC_C
Cuts at: 3709 3709
Size: 6388
AclI AA'CG_TT
Cuts at: 1563 4515 4888 1563
Size: 2952 373 3063
Fragments arranged by size:
3063 2952 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2294 2579 2823 3144 3276 4451 5835 6103
Size: 662 285 244 321 132 1175 1384 268
Cuts at: 6103 1632
Size: 1917
Fragments arranged by size:
1917 1384 1175 662 321 285 268 244
132
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6191
AflIII A'CryG_T
Cuts at: 1203 2056 2763 5287 6264 1203
Size: 853 707 2524 977 1327
Fragments arranged by size:
2524 1327 977 853 707
AgeI A'CCGG_T
Cuts at: 5219 5219
Size: 6388
AhdI GACnn_n'nnGTC
Cuts at: 5117 5117
Size: 6388
AleI CACnn'nnGTG
Cuts at: 1166 3101 1166
Size: 1935 4453
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2066 1184
Size: 882 5506
AlwNI CAG_nnn'CTG
Cuts at: 2473 2512 2890 3272 3299 3574 4131 5703 2473
Size: 39 378 382 27 275 557 1572 3158
Fragments arranged by size:
3158 1572 557 382 378 275 39 27
ApaLI G'TGCA_C
Cuts at: 4445 5601 4445
Size: 1156 5232
ApoI r'AATT_y
Cuts at: 1493 3697 3923 4075 6184 1493
Size: 2204 226 152 2109 1697
Fragments arranged by size:
2204 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4942 160
Size: 872 3910 1606
Fragments arranged by size:
3910 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6388
AvaI C'yCGr_G
Cuts at: 1870 1943 2106 2414 3713 1870
Size: 73 163 308 1299 4545
Fragments arranged by size:
4545 1299 308 163 73
AvrII C'CTAG_G
Cuts at: 3329 3329
Size: 6388
BamHI G'GATC_C
Cuts at: 3119 3718 4085 3119
Size: 599 367 5422
Fragments arranged by size:
5422 599 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2663 3615 3709 5164 618
Size: 325 217 627 876 952 94 1455 1842
Fragments arranged by size:
1842 1455 952 876 627 325 217 94
BanII G_rGCy'C
Cuts at: 1391 2119 2128 2415 2421 2979 3707 1391
Size: 728 9 287 6 558 728 4072
Fragments arranged by size:
4072 728 728 558 287 9 6
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2811 3667 928
Size: 971 912 856 3649
Fragments arranged by size:
3649 971 912 856
BbvCI CC'TCA_GC
Cuts at: 3087 3135 3087
Size: 48 6340
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4579 4613 1903
Size: 34 2642 34 3678
Fragments arranged by size:
3678 2642 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2059 4280 5490 2059
Size: 2221 1210 2957
Fragments arranged by size:
2957 2221 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6388
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4999 136
Size: 107 122 71 631 605 3327 1525
Fragments arranged by size:
3327 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6383 6383
Size: 6388
BlpI GC'TnA_GC
Cuts at: 2811 3805 2811
Size: 994 5394
Bme1580I G_kGCm'C
Cuts at: 1790 2244 4449 5605 1790
Size: 454 2205 1156 2573
Fragments arranged by size:
2573 2205 1156 454
BmgBI CAC'GTC
Cuts at: 1318 2659 1318
Size: 1341 5047
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2805 2878 4119
Size: 416 53 83 399 24 1388 73 1241
Cuts at: 4119 5077 442
Size: 958 1753
Fragments arranged by size:
1753 1388 1241 958 416 399 83 73
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6388
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3193 3223 3456 3193
Size: 30 233 6125
Fragments arranged by size:
6125 233 30
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2133 2350 2414 2421 2622 2979 3193 3212
Size: 894 217 64 7 201 357 214 19
Cuts at: 3212 3518 3569 3647 5048 1239
Size: 306 51 78 1401 2579
Fragments arranged by size:
2579 1401 894 357 306 217 214 201
78 64 51 19 7
Bpu10I CC'TnA_GC
Cuts at: 2409 2936 3087 3135 2409
Size: 527 151 48 5662
Fragments arranged by size:
5662 527 151 48
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2517 3275 3464 4513 5378 5676 2517
Size: 758 189 1049 865 298 3229
Fragments arranged by size:
3229 1049 865 758 298 189
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5051 882
Size: 617 3552 2219
Fragments arranged by size:
3552 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3601 4179 493
Size: 711 2397 578 2702
Fragments arranged by size:
2702 2397 711 578
BsaBI GATnn'nnATC
Cuts at: 6382 6382
Size: 6388
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4195 4577 5212 275
Size: 53 83 186 936 2662 382 635 1451
Fragments arranged by size:
2662 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4821 5219 5493 5640 6051 4821
Size: 398 274 147 411 5158
Fragments arranged by size:
5158 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2015 2045 2419 2449 1178
Size: 30 165 30 612 30 374 30 5117
Fragments arranged by size:
5117 612 374 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2691 2921 1186
Size: 1505 230 4653
Fragments arranged by size:
4653 1505 230
BseYI C'CCAG_C
Cuts at: 2727 3068 3265 5591 6087 2727
Size: 341 197 2326 496 3028
Fragments arranged by size:
3028 2326 496 341 197
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2630 2767 3581 1163
Size: 573 894 137 814 3970
Fragments arranged by size:
3970 894 814 573 137
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2827 3838 4599 5627 1540
Size: 275 186 826 1011 761 1028 2301
Fragments arranged by size:
2301 1028 1011 826 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2107 2415 2942 3061 4449 4534 5605 2107
Size: 308 527 119 1388 85 1071 2890
Fragments arranged by size:
2890 1388 1071 527 308 119 85
BsmI GAATG_Cn'
Cuts at: 2545 3899 3992 2545
Size: 1354 93 4941
Fragments arranged by size:
4941 1354 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2107 2119 2128 2244 2415 2421 2942
Size: 399 317 12 9 116 171 6 521
Cuts at: 2942 2979 3061 3707 4449 4534 5605 1391
Size: 37 82 646 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 646 521 399 317 171
116 85 82 37 12 9 6
BspEI T'CCGG_A
Cuts at: 6051 6051
Size: 6388
BspHI T'CATG_A
Cuts at: 4277 6007 4277
Size: 1730 4658
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3627 3743 844
Size: 2783 116 3489
Fragments arranged by size:
3489 2783 116
BsrBI CCG'CTC
Cuts at: 1394 1823 3613 4275 1394
Size: 429 1790 662 3507
Fragments arranged by size:
3507 1790 662 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4883 5057 65
Size: 1183 3635 174 1396
Fragments arranged by size:
3635 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2822 3344 3633 5032 5219 1700
Size: 110 1012 522 289 1399 187 2869
Fragments arranged by size:
2869 1399 1012 522 289 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6388
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6388
BssSI C'ACGA_G
Cuts at: 4448 5460 4448
Size: 1012 5376
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6388
BstBI TT'CG_AA
Cuts at: 5192 5192
Size: 6388
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6388
BstXI CCAn_nnnn'nTGG
Cuts at: 2431 3377 2431
Size: 946 5442
BstYI r'GATC_y
Cuts at: 1972 2564 2677 3119 3364 3718 4085 4470 4487
Size: 592 113 442 245 354 367 385 17
Cuts at: 4487 5939 6025 6037 6383 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 592 442 385 367 354 346
245 113 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2087 2194 2745 3426 513
Size: 552 939 83 107 551 681 3475
Fragments arranged by size:
3475 939 681 552 551 107 83
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5649
Fragments arranged by size:
5649 683 56
BtsI GCAGTG_nn'
Cuts at: 2848 2953 3593 3904 4698 4718 2848
Size: 105 640 311 794 20 4518
Fragments arranged by size:
4518 794 640 311 105 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6388
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6353
DraI TTT'AAA
Cuts at: 3692 3692
Size: 6388
DraIII CAC_nnn'GTG
Cuts at: 3394 3394
Size: 6388
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5395 809
Size: 507 4079 1802
Fragments arranged by size:
4079 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2161 3520 3835
Size: 54 1425 50 275 18 331 1359 315
Cuts at: 3835 4724 6353 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1359 889 331 315 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3835 1537
Size: 275 2023 4090
Fragments arranged by size:
4090 2023 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2557 2598 2991 3075 4318 1353
Size: 1204 41 393 84 1243 3423
Fragments arranged by size:
3423 1243 1204 393 84 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4975 5349 5495 387
Size: 1064 138 3386 374 146 1280
Fragments arranged by size:
3386 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2413 2413
Size: 6388
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2133 2294 2350 2414 2421 2579 2622
Size: 393 501 161 56 64 7 158 43
Cuts at: 2622 2823 2979 3144 3193 3212 3276 3518 3569
Size: 201 156 165 49 19 64 242 51
Cuts at: 3569 3647 4451 5048 5835 6103 1239
Size: 78 804 597 787 268 1524
Fragments arranged by size:
1524 804 787 597 501 393 268 242
201 165 161 158 156 78 64 64
56 51 49 43 19 7
EcoNI CCTnn'n_nnAGG
Cuts at: 3181 3181
Size: 6388
EcoO109I rG'GnC_Cy
Cuts at: 3065 3333 3354 3544 3571 3065
Size: 268 21 190 27 5882
Fragments arranged by size:
5882 268 190 27 21
EcoRI G'AATT_C
Cuts at: 3697 4075 3697
Size: 378 6010
FspI TGC'GCA
Cuts at: 4894 4894
Size: 6388
HaeII r_GCGC'y
Cuts at: 1678 3272 5535 1678
Size: 1594 2263 2531
Fragments arranged by size:
2531 2263 1594
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2015 2047 3575 3607 5029
Size: 32 135 32 443 32 1528 32 1422
Cuts at: 5029 5061 5103 5135 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1528 1422 443 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3732 3978 669
Size: 988 281 1794 246 3079
Fragments arranged by size:
3079 1794 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6388
HpaI GTT'AAC
Cuts at: 3978 3978
Size: 6388
KpnI G_GTAC'C
Cuts at: 3713 3713
Size: 6388
MfeI C'AATT_G
Cuts at: 3987 3987
Size: 6388
MluI A'CGCG_T
Cuts at: 2056 6264 2056
Size: 4208 2180
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2383 2697 5502 5686 1305
Size: 1078 314 2805 184 2007
Fragments arranged by size:
2805 2007 1078 314 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2163 10
Size: 54 1425 343 331 4235
Fragments arranged by size:
4235 1425 343 331 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2312 3101 3486 4346 4705 4864 518
Size: 648 1146 789 385 860 359 159 2042
Fragments arranged by size:
2042 1146 860 789 648 385 359 159
MspA1I CmG'CkG
Cuts at: 1606 2072 2089 3640 3804 4481 5629 5874 1606
Size: 466 17 1551 164 677 1148 245 2120
Fragments arranged by size:
2120 1551 1148 677 466 245 164 17
NcoI C'CATG_G
Cuts at: 513 1065 2004 2194 2745 3426 513
Size: 552 939 190 551 681 3475
Fragments arranged by size:
3475 939 681 552 551 190
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6388
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6388
NotI GC'GGCC_GC
Cuts at: 3835 3835
Size: 6388
NspI r_CATG'y
Cuts at: 1132 1229 3746 5291 1132
Size: 97 2517 1545 2229
Fragments arranged by size:
2517 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2870 3124 1348
Size: 1522 254 4612
Fragments arranged by size:
4612 1522 254
PciI A'CATG_T
Cuts at: 5287 5287
Size: 6388
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2890 3272 1108
Size: 1782 382 4224
Fragments arranged by size:
4224 1782 382
PmeI GTTT'AAAC
Cuts at: 3692 3692
Size: 6388
PmlI CAC'GTG
Cuts at: 3601 3601
Size: 6388
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4457 4489 5970 6002 4457
Size: 32 1481 32 4843
Fragments arranged by size:
4843 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3065 3544 3065
Size: 479 5909
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6388
PsiI TTA'TAA
Cuts at: 3958 3958
Size: 6388
PspXI vC'TCGA_Gb
Cuts at: 2414 2414
Size: 6388
PstI C_TGCA'G
Cuts at: 830 2859 3283 3304 3740 830
Size: 2029 424 21 436 3478
Fragments arranged by size:
3478 2029 436 424 21
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6388
PvuII CAG'CTG
Cuts at: 3640 3640
Size: 6388
SacI G_AGCT'C
Cuts at: 2415 2415
Size: 6388
SacII CC_GC'GG
Cuts at: 2090 2090
Size: 6388
SalI G'TCGA_C
Cuts at: 1936 3730 1936
Size: 1794 4594
SbfI CC_TGCA'GG
Cuts at: 3740 3740
Size: 6388
ScaI AGT'ACT
Cuts at: 4636 6348 4636
Size: 1712 4676
SfcI C'TryA_G
Cuts at: 826 1045 2652 2855 3279 3300 3736 4871 5552
Size: 219 1607 203 424 21 436 1135 681
Cuts at: 5552 5743 826
Size: 191 1471
Fragments arranged by size:
1607 1471 1135 681 436 424 219 203
191 21
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6388
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6388
SmaI CCC'GGG
Cuts at: 1872 3715 1872
Size: 1843 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2414 2532 3254 3443 4492 5393
Size: 197 926 471 118 722 189 1049 901
Cuts at: 5393 5655 820
Size: 262 1553
Fragments arranged by size:
1553 1049 926 901 722 471 262 197
189 118
SnaBI TAC'GTA
Cuts at: 493 4179 493
Size: 3686 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6388
SphI G_CATG'C
Cuts at: 1132 3746 1132
Size: 2614 3774
SspI AAT'ATT
Cuts at: 5 52 3473 4312 5
Size: 47 3421 839 2081
Fragments arranged by size:
3421 2081 839 47
StuI AGG'CCT
Cuts at: 1517 2617 1517
Size: 1100 5288
StyI C'CwwG_G
Cuts at: 513 1065 2004 2194 2745 3329 3426 3827 513
Size: 552 939 190 551 584 97 401 3074
Fragments arranged by size:
3074 939 584 552 551 401 190 97
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2651 2741 4415 4432 4585 4770 5215
Size: 1255 1071 90 1674 17 153 185 445
Cuts at: 5215 325
Size: 1498
Fragments arranged by size:
1674 1498 1255 1071 445 185 153 90
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4634 6346 96
Size: 274 80 33 51 1443 2657 1712 138
Fragments arranged by size:
2657 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2143 2175 6348 6380 2143
Size: 32 4173 32 2151
Fragments arranged by size:
4173 2151 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3587 1316
Size: 2271 4117
XbaI T'CTAG_A
Cuts at: 3724 3724
Size: 6388
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2242 3424 2242
Size: 1182 5206
XhoI C'TCGA_G
Cuts at: 1943 2414 1943
Size: 471 5917
XmaI C'CCGG_G
Cuts at: 1870 3713 1870
Size: 1843 4545
XmnI GAAnn'nnTTC
Cuts at: 4517 4517
Size: 6388
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4196 276
Size: 53 83 186 936 2662 2468
Fragments arranged by size:
2662 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI
PvuII SacI SacII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BbeI BfrBI BsiWI
BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PspOMI
PsrI RsrII SanDI SapI SexAI SfiI SfoI SrfI
SwaI