Restriction map for
pFN21AE9250
(Circular) (Six-base) MAPSORT of: pFN21AE9250.seq Check: 677 from: 1 to: 5764
pFN21AE9250 5764 bp
With 182 enzymes: SgfI *
March 28, 2012 12:16 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3574 278
Size: 53 83 186 936 2038 2468
Fragments arranged by size:
2468 2038 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3107 1937
Size: 1170 4594
Acc65I G'GTAC_C
Cuts at: 3085 3085
Size: 5764
AclI AA'CG_TT
Cuts at: 1563 3891 4264 1563
Size: 2328 373 3063
Fragments arranged by size:
3063 2328 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2099 2324 2356 3827 5211 5479 1632
Size: 467 225 32 1471 1384 268 1917
Fragments arranged by size:
1917 1471 1384 467 268 225 32
AflII C'TTAA_G
Cuts at: 820 1017 2606 820
Size: 197 1589 3978
Fragments arranged by size:
3978 1589 197
AflIII A'CryG_T
Cuts at: 1203 4663 5640 1203
Size: 3460 977 1327
Fragments arranged by size:
3460 1327 977
AgeI A'CCGG_T
Cuts at: 4595 4595
Size: 5764
AhdI GACnn_n'nnGTC
Cuts at: 4493 4493
Size: 5764
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5764
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2965 1184
Size: 1781 3983
AlwNI CAG_nnn'CTG
Cuts at: 2539 3507 5079 2539
Size: 968 1572 3224
Fragments arranged by size:
3224 1572 968
ApaLI G'TGCA_C
Cuts at: 3821 4977 3821
Size: 1156 4608
ApoI r'AATT_y
Cuts at: 1493 2122 2320 2334 2798 2825 3073 3299 3451
Size: 629 198 14 464 27 248 226 152
Cuts at: 3451 5560 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 629 464 248 226 198 152
27 14
AseI AT'TA_AT
Cuts at: 160 1032 4318 160
Size: 872 3286 1606
Fragments arranged by size:
3286 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5764
AvaI C'yCGr_G
Cuts at: 1870 1943 3007 3089 1870
Size: 73 1064 82 4545
Fragments arranged by size:
4545 1064 82 73
BamHI G'GATC_C
Cuts at: 2955 3094 3461 2955
Size: 139 367 5258
Fragments arranged by size:
5258 367 139
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3085 4540 618
Size: 325 217 627 1298 1455 1842
Fragments arranged by size:
1842 1455 1298 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3083 1391
Size: 1692 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4793
BbvCI CC'TCA_GC
Cuts at: 2507 2507
Size: 5764
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3955 3989 1903
Size: 34 2018 34 3678
Fragments arranged by size:
3678 2018 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3063 3656 4866 3063
Size: 593 1210 3961
Fragments arranged by size:
3961 1210 593
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5764
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4375 136
Size: 107 122 71 631 605 2703 1525
Fragments arranged by size:
2703 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5759 5759
Size: 5764
BlpI GC'TnA_GC
Cuts at: 3181 3181
Size: 5764
Bme1580I G_kGCm'C
Cuts at: 1790 3825 4981 1790
Size: 2035 1156 2573
Fragments arranged by size:
2573 2035 1156
BmgBI CAC'GTC
Cuts at: 1318 2493 1318
Size: 1175 4589
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3495 4453 442
Size: 416 53 83 399 24 2078 958 1753
Fragments arranged by size:
2078 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2553 1056
Size: 1497 4267
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4424 1239
Size: 3185 2579
Bpu10I CC'TnA_GC
Cuts at: 2507 2929 2507
Size: 422 5342
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2745 3889 4754 5052 2745
Size: 1144 865 298 3457
Fragments arranged by size:
3457 1144 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4427 882
Size: 617 2928 2219
Fragments arranged by size:
2928 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2458 3555 493
Size: 711 1254 1097 2702
Fragments arranged by size:
2702 1254 1097 711
BsaBI GATnn'nnATC
Cuts at: 5758 5758
Size: 5764
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3571 3953 4588 275
Size: 53 83 186 936 2038 382 635 1451
Fragments arranged by size:
2038 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4197 4595 4869 5016 5427 4197
Size: 398 274 147 411 4534
Fragments arranged by size:
4534 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5539
Fragments arranged by size:
5539 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2399 2488 1186
Size: 1213 89 4462
Fragments arranged by size:
4462 1213 89
BseYI C'CCAG_C
Cuts at: 4967 5463 4967
Size: 496 5268
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5191
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3214 3975 5003 1540
Size: 275 186 1213 761 1028 2301
Fragments arranged by size:
2301 1213 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3825 3910 4981 3825
Size: 85 1071 4608
Fragments arranged by size:
4608 1071 85
BsiWI C'GTAC_G
Cuts at: 2481 2481
Size: 5764
BsmI GAATG_Cn'
Cuts at: 2395 3275 3368 2395
Size: 880 93 4791
Fragments arranged by size:
4791 880 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3083 3825 3910 4981 1391
Size: 399 1293 742 85 1071 2174
Fragments arranged by size:
2174 1293 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5427 5427
Size: 5764
BspHI T'CATG_A
Cuts at: 2719 3653 5383 2719
Size: 934 1730 3100
Fragments arranged by size:
3100 1730 934
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3119 844
Size: 2275 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3651 1394
Size: 429 1828 3507
Fragments arranged by size:
3507 1828 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4259 4433 65
Size: 1183 3011 174 1396
Fragments arranged by size:
3011 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4408 4595 1700
Size: 110 2598 187 2869
Fragments arranged by size:
2869 2598 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5764
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5764
BssSI C'ACGA_G
Cuts at: 3824 4836 3824
Size: 1012 4752
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5764
BstBI TT'CG_AA
Cuts at: 2404 4568 2404
Size: 2164 3600
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5764
BstYI r'GATC_y
Cuts at: 1972 2955 3094 3461 3846 3863 5315 5401 5413
Size: 983 139 367 385 17 1452 86 12
Cuts at: 5413 5759 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 983 385 367 346 139 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4273
Fragments arranged by size:
4273 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5025
Fragments arranged by size:
5025 683 56
BtsI GCAGTG_nn'
Cuts at: 3280 4074 4094 3280
Size: 794 20 4950
Fragments arranged by size:
4950 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5764
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5729
DraI TTT'AAA
Cuts at: 2317 2623 2841 3068 2317
Size: 306 218 227 5013
Fragments arranged by size:
5013 306 227 218
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4771 809
Size: 507 3455 1802
Fragments arranged by size:
3455 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3211 4100 5729
Size: 54 1425 50 275 18 1381 889 1629
Cuts at: 5729 8
Size: 43
Fragments arranged by size:
1629 1425 1381 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3211 1537
Size: 275 1399 4090
Fragments arranged by size:
4090 1399 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3694 1353
Size: 2341 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4351 4725 4871 387
Size: 1064 138 2762 374 146 1280
Fragments arranged by size:
2762 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2099 2324 2356 3827 4424 5211 5479
Size: 393 467 225 32 1471 597 787 268
Cuts at: 5479 1239
Size: 1524
Fragments arranged by size:
1524 1471 787 597 467 393 268 225
32
EcoO109I rG'GnC_Cy
Cuts at: 2997 3004 2997
Size: 7 5757
EcoRI G'AATT_C
Cuts at: 3073 3451 3073
Size: 378 5386
FspI TGC'GCA
Cuts at: 4270 4270
Size: 5764
HaeII r_GCGC'y
Cuts at: 1678 2540 4911 1678
Size: 862 2371 2531
Fragments arranged by size:
2531 2371 862
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2081 2113 4405 4437 4479
Size: 32 135 32 509 32 2292 32 42
Cuts at: 4479 4511 1373
Size: 32 2626
Fragments arranged by size:
2626 2292 509 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2293 3108 3354 669
Size: 988 281 355 815 246 3079
Fragments arranged by size:
3079 988 815 355 281 246
HindIII A'AGCT_T
Cuts at: 748 2813 748
Size: 2065 3699
HpaI GTT'AAC
Cuts at: 3354 3354
Size: 5764
KpnI G_GTAC'C
Cuts at: 3089 3089
Size: 5764
MfeI C'AATT_G
Cuts at: 2544 3363 2544
Size: 819 4945
MluI A'CGCG_T
Cuts at: 5640 5640
Size: 5764
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2152 2773 4878 5062 1305
Size: 847 621 2105 184 2007
Fragments arranged by size:
2105 2007 847 621 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3942
Fragments arranged by size:
3942 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2169 2222 3722 4081 4240 518
Size: 648 1003 53 1500 359 159 2042
Fragments arranged by size:
2042 1500 1003 648 359 159 53
MspA1I CmG'CkG
Cuts at: 1606 2939 3015 3180 3857 5005 5250 1606
Size: 1333 76 165 677 1148 245 2120
Fragments arranged by size:
2120 1333 1148 677 245 165 76
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4273
Fragments arranged by size:
4273 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5764
NheI G'CTAG_C
Cuts at: 1052 2549 1052
Size: 1497 4267
NotI GC'GGCC_GC
Cuts at: 3211 3211
Size: 5764
NspI r_CATG'y
Cuts at: 1132 1229 3122 4667 1132
Size: 97 1893 1545 2229
Fragments arranged by size:
2229 1893 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5764
PciI A'CATG_T
Cuts at: 4663 4663
Size: 5764
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5764
PmeI GTTT'AAAC
Cuts at: 3068 3068
Size: 5764
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3833 3865 5346 5378 3833
Size: 32 1481 32 4219
Fragments arranged by size:
4219 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2997 3004 2997
Size: 7 5757
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5764
PsiI TTA'TAA
Cuts at: 3334 3334
Size: 5764
PstI C_TGCA'G
Cuts at: 830 2115 3116 830
Size: 1285 1001 3478
Fragments arranged by size:
3478 1285 1001
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5764
PvuII CAG'CTG
Cuts at: 3015 3015
Size: 5764
SalI G'TCGA_C
Cuts at: 1936 3106 1936
Size: 1170 4594
SanDI GG'GwC_CC
Cuts at: 2997 2997
Size: 5764
SbfI CC_TGCA'GG
Cuts at: 3116 3116
Size: 5764
ScaI AGT'ACT
Cuts at: 4012 5724 4012
Size: 1712 4052
SexAI A'CCwGG_T
Cuts at: 2278 2278
Size: 5764
SfcI C'TryA_G
Cuts at: 826 1045 2111 2145 2669 3112 4247 4928 5119
Size: 219 1066 34 524 443 1135 681 191
Cuts at: 5119 826
Size: 1471
Fragments arranged by size:
1471 1135 1066 681 524 443 219 191
34
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5764
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5764
SmaI CCC'GGG
Cuts at: 1872 3091 1872
Size: 1219 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2606 2760 3868 4769 5031 820
Size: 197 926 663 154 1108 901 262 1553
Fragments arranged by size:
1553 1108 926 901 663 262 197 154
SnaBI TAC'GTA
Cuts at: 493 3555 493
Size: 3062 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5764
SphI G_CATG'C
Cuts at: 1132 3122 1132
Size: 1990 3774
SspI AAT'ATT
Cuts at: 5 52 3688 5
Size: 47 3636 2081
Fragments arranged by size:
3636 2081 47
StuI AGG'CCT
Cuts at: 1517 2084 1517
Size: 567 5197
StyI C'CwwG_G
Cuts at: 513 1065 2004 2683 3203 513
Size: 552 939 679 520 3074
Fragments arranged by size:
3074 939 679 552 520
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3791 3808 3961 4146 4591 325
Size: 1255 2211 17 153 185 445 1498
Fragments arranged by size:
2211 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2424 2442 2463
Size: 274 80 33 51 1443 447 18 21
Cuts at: 2463 4010 5722 96
Size: 1547 1712 138
Fragments arranged by size:
1712 1547 1443 447 274 138 80 51
33 21 18
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5724 5756 5724
Size: 32 5732
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5764
XbaI T'CTAG_A
Cuts at: 3100 3100
Size: 5764
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5764
XmaI C'CCGG_G
Cuts at: 1870 3089 1870
Size: 1219 4545
XmnI GAAnn'nnTTC
Cuts at: 3893 3893
Size: 5764
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3572 276
Size: 53 83 186 936 2038 2468
Fragments arranged by size:
2468 2038 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SanDI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacI SacII SapI SfiI SfoI SrfI
SwaI XcmI