Restriction map for
pFN21AE9244
(Circular) (Six-base) MAPSORT of: pFN21AE9244.seq Check: 4165 from: 1 to: 9667
pFN21AE9244 9667 bp
With 182 enzymes: SgfI *
October 3, 2019 17:59 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7477 278
Size: 53 83 186 936 5941 2468
Fragments arranged by size:
5941 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5228 7010 1937
Size: 3291 1782 4594
Fragments arranged by size:
4594 3291 1782
Acc65I G'GTAC_C
Cuts at: 6988 6988
Size: 9667
AclI AA'CG_TT
Cuts at: 1563 7794 8167 1563
Size: 6231 373 3063
Fragments arranged by size:
6231 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2361 2388 2711 3656 3948 4737 4790 5018
Size: 729 27 323 945 292 789 53 228
Cuts at: 5018 5490 5928 6861 7730 9114 9382 1632
Size: 472 438 933 869 1384 268 1917
Fragments arranged by size:
1917 1384 945 933 869 789 729 472
438 323 292 268 228 53 27
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9470
AflIII A'CryG_T
Cuts at: 1203 8566 9543 1203
Size: 7363 977 1327
Fragments arranged by size:
7363 1327 977
AgeI A'CCGG_T
Cuts at: 8498 8498
Size: 9667
AhdI GACnn_n'nnGTC
Cuts at: 3279 8396 3279
Size: 5117 4550
AleI CACnn'nnGTG
Cuts at: 1166 5190 1166
Size: 4024 5643
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 6333 1184
Size: 5149 4518
AlwNI CAG_nnn'CTG
Cuts at: 3031 5468 7410 8982 3031
Size: 2437 1942 1572 3716
Fragments arranged by size:
3716 2437 1942 1572
ApaLI G'TGCA_C
Cuts at: 4456 7724 8880 4456
Size: 3268 1156 5243
Fragments arranged by size:
5243 3268 1156
ApoI r'AATT_y
Cuts at: 1493 2277 2483 2513 2799 3142 3444 3885 6161
Size: 784 206 30 286 343 302 441 2276
Cuts at: 6161 6976 7202 7354 9463 1493
Size: 815 226 152 2109 1697
Fragments arranged by size:
2276 2109 1697 815 784 441 343 302
286 226 206 152 30
AseI AT'TA_AT
Cuts at: 160 1032 3264 4974 5498 6905 8221 160
Size: 872 2232 1710 524 1407 1316 1606
Fragments arranged by size:
2232 1710 1606 1407 1316 872 524
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9667
AvaI C'yCGr_G
Cuts at: 1870 1943 6992 1870
Size: 73 5049 4545
Fragments arranged by size:
5049 4545 73
BamHI G'GATC_C
Cuts at: 5106 6140 6997 7364 5106
Size: 1034 857 367 7409
Fragments arranged by size:
7409 1034 857 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 6988 8443 618
Size: 325 217 627 5201 1455 1842
Fragments arranged by size:
5201 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2157 5367 6986 1391
Size: 766 3210 1619 4072
Fragments arranged by size:
4072 3210 1619 766
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2349 2595 3186 3603 3828 4256 4370
Size: 971 450 246 591 417 225 428 114
Cuts at: 4370 5007 5142 928
Size: 637 135 5453
Fragments arranged by size:
5453 971 637 591 450 428 417 246
225 135 114
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7858 7892 1903
Size: 34 5921 34 3678
Fragments arranged by size:
5921 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7559 8769 7559
Size: 1210 8457
BclI T'GATC_A
Cuts at: 1548 2979 4957 1548
Size: 1431 1978 6258
Fragments arranged by size:
6258 1978 1431
BfrBI ATG'CAT
Cuts at: 6393 6397 6393
Size: 4 9663
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 8278 136
Size: 107 122 71 631 605 6606 1525
Fragments arranged by size:
6606 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3742 9662 3742
Size: 5920 3747
BlpI GC'TnA_GC
Cuts at: 3345 5433 7084 3345
Size: 2088 1651 5928
Fragments arranged by size:
5928 2088 1651
Bme1580I G_kGCm'C
Cuts at: 1790 3310 4460 5393 7728 8884 1790
Size: 1520 1150 933 2335 1156 2573
Fragments arranged by size:
2573 2335 1520 1156 1150 933
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9667
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2544 7398 8356
Size: 416 53 83 399 24 1127 4854 958
Cuts at: 8356 442
Size: 1753
Fragments arranged by size:
4854 1753 1127 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 5856 1056
Size: 4800 4867
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2133 2141 2316 2780 2957 6501 8327 1239
Size: 894 8 175 464 177 3544 1826 2579
Fragments arranged by size:
3544 2579 1826 894 464 177 175 8
Bpu10I CC'TnA_GC
Cuts at: 2208 4866 2208
Size: 2658 7009
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4838 7792 8657 8955 4838
Size: 2954 865 298 5550
Fragments arranged by size:
5550 2954 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4687 5397 8330 882
Size: 617 3188 710 2933 2219
Fragments arranged by size:
3188 2933 2219 710 617
BsaAI yAC'GTr
Cuts at: 493 1204 3208 4073 6771 7458 493
Size: 711 2004 865 2698 687 2702
Fragments arranged by size:
2702 2698 2004 865 711 687
BsaBI GATnn'nnATC
Cuts at: 2433 5988 9661 2433
Size: 3555 3673 2439
Fragments arranged by size:
3673 3555 2439
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 7474 7856 8491 275
Size: 53 83 186 936 5941 382 635 1451
Fragments arranged by size:
5941 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6677 8100 8498 8772 8919 9330 6677
Size: 1423 398 274 147 411 7014
Fragments arranged by size:
7014 1423 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2126 2156 4310 4340 1178
Size: 30 165 30 723 30 2154 30 6505
Fragments arranged by size:
6505 2154 723 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2134 3302 6088 1186
Size: 948 1168 2786 4765
Fragments arranged by size:
4765 2786 1168 948
BseYI C'CCAG_C
Cuts at: 3664 4603 8870 9366 3664
Size: 939 4267 496 3965
Fragments arranged by size:
4267 3965 939 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4211 4234 1163
Size: 573 2475 23 6596
Fragments arranged by size:
6596 2475 573 23
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7117 7878 8906 1540
Size: 275 186 5116 761 1028 2301
Fragments arranged by size:
5116 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2157 4460 5187 5367 7728 7813 8884 2157
Size: 2303 727 180 2361 85 1071 2940
Fragments arranged by size:
2940 2361 2303 1071 727 180 85
BsmI GAATG_Cn'
Cuts at: 5851 6310 7178 7271 5851
Size: 459 868 93 8247
Fragments arranged by size:
8247 868 459 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2157 3310 4460 5187 5367 5393 6986
Size: 399 367 1153 1150 727 180 26 1593
Cuts at: 6986 7728 7813 8884 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1593 1153 1150 1071 742 727 399
367 180 85 26
BspEI T'CCGG_A
Cuts at: 9330 9330
Size: 9667
BspHI T'CATG_A
Cuts at: 7556 9286 7556
Size: 1730 7937
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5422 7022 844
Size: 4578 1600 3489
Fragments arranged by size:
4578 3489 1600
BsrBI CCG'CTC
Cuts at: 1394 1823 7554 1394
Size: 429 5731 3507
Fragments arranged by size:
5731 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4359 8162 8336 65
Size: 1183 3111 3803 174 1396
Fragments arranged by size:
3803 3111 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4941 8311 8498 1700
Size: 110 3131 3370 187 2869
Fragments arranged by size:
3370 3131 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3586 6607 96
Size: 3490 3021 3156
Fragments arranged by size:
3490 3156 3021
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9667
BssSI C'ACGA_G
Cuts at: 7727 8739 7727
Size: 1012 8655
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2242 4094 794
Size: 1448 1852 6367
Fragments arranged by size:
6367 1852 1448
BstBI TT'CG_AA
Cuts at: 2658 8471 2658
Size: 5813 3854
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9667
BstXI CCAn_nnnn'nTGG
Cuts at: 2618 5393 6775 2618
Size: 2775 1382 5510
Fragments arranged by size:
5510 2775 1382
BstYI r'GATC_y
Cuts at: 1972 2521 3742 5106 6140 6997 7364 7749 7766
Size: 549 1221 1364 1034 857 367 385 17
Cuts at: 7766 9218 9304 9316 9662 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1364 1221 1034 857 549 385
367 346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 6340 6340
Size: 9667
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 9115
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4612 487
Size: 683 56 3386 5542
Fragments arranged by size:
5542 3386 683 56
BtsI GCAGTG_nn'
Cuts at: 2029 3308 5617 5930 6574 7183 7977 7997 2029
Size: 1279 2309 313 644 609 794 20 3699
Fragments arranged by size:
3699 2309 1279 794 644 609 313 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9667
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9632
DraI TTT'AAA
Cuts at: 6435 6971 6435
Size: 536 9131
DraIII CAC_nnn'GTG
Cuts at: 3754 3754
Size: 9667
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8674 809
Size: 507 7358 1802
Fragments arranged by size:
7358 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5358 5395 7114
Size: 54 1425 50 275 18 3528 37 1719
Cuts at: 7114 8003 9632 8
Size: 889 1629 43
Fragments arranged by size:
3528 1719 1629 1425 889 275 54 50
43 37 18
EagI C'GGCC_G
Cuts at: 1537 1812 7114 1537
Size: 275 5302 4090
Fragments arranged by size:
5302 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2049 2061 3111 3603 4440 4730 4782 4809
Size: 696 12 1050 492 837 290 52 27
Cuts at: 4809 5466 7597 1353
Size: 657 2131 3423
Fragments arranged by size:
3423 2131 1050 837 696 657 492 290
52 27 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 8254 8628 8774 387
Size: 1064 138 6665 374 146 1280
Fragments arranged by size:
6665 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2155 5365 2155
Size: 3210 6457
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2133 2141 2316 2361 2388 2711 2780
Size: 393 501 8 175 45 27 323 69
Cuts at: 2780 2957 3656 3948 4737 4790 5018 5490 5928
Size: 177 699 292 789 53 228 472 438
Cuts at: 5928 6501 6861 7730 8327 9114 9382 1239
Size: 573 360 869 597 787 268 1524
Fragments arranged by size:
1524 869 789 787 699 597 573 501
472 438 393 360 323 292 268 228
177 175 69 53 45 27 8
EcoNI CCTnn'n_nnAGG
Cuts at: 2370 6237 2370
Size: 3867 5800
EcoO109I rG'GnC_Cy
Cuts at: 2546 6535 2546
Size: 3989 5678
EcoRI G'AATT_C
Cuts at: 6161 6976 7354 6161
Size: 815 378 8474
Fragments arranged by size:
8474 815 378
EcoRV GAT'ATC
Cuts at: 2807 4701 2807
Size: 1894 7773
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4384 5324 4384
Size: 940 8727
FspI TGC'GCA
Cuts at: 5771 8173 5771
Size: 2402 7265
HaeII r_GCGC'y
Cuts at: 1678 8814 1678
Size: 7136 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2126 2129 2158 2161 3173
Size: 32 135 32 554 3 29 3 1012
Cuts at: 3173 3205 3265 3297 4310 4342 4786 4818 8308
Size: 32 60 32 1013 32 444 32 3490
Cuts at: 8308 8340 8382 8414 1373
Size: 32 42 32 2626
Fragments arranged by size:
3490 2626 1013 1012 554 444 135 60
42 32 32 32 32 32 32 32
32 29 3 3
HincII GTy'rAC
Cuts at: 669 1657 1938 3273 3556 5229 7011 7257 669
Size: 988 281 1335 283 1673 1782 246 3079
Fragments arranged by size:
3079 1782 1673 1335 988 283 281 246
HindIII A'AGCT_T
Cuts at: 748 3016 748
Size: 2268 7399
HpaI GTT'AAC
Cuts at: 7257 7257
Size: 9667
KpnI G_GTAC'C
Cuts at: 6992 6992
Size: 9667
MfeI C'AATT_G
Cuts at: 2213 7266 2213
Size: 5053 4614
MluI A'CGCG_T
Cuts at: 9543 9543
Size: 9667
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4140 5134 5818 8781 8965 1305
Size: 2835 994 684 2963 184 2007
Fragments arranged by size:
2963 2835 2007 994 684 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 5397 10
Size: 54 1425 343 3565 4280
Fragments arranged by size:
4280 3565 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3092 3486 5190 6361 7625 7984 8143
Size: 648 1926 394 1704 1171 1264 359 159
Cuts at: 8143 518
Size: 2042
Fragments arranged by size:
2042 1926 1704 1264 1171 648 394 359
159
MspA1I CmG'CkG
Cuts at: 1606 3031 3361 6317 7083 7760 8908 9153 1606
Size: 1425 330 2956 766 677 1148 245 2120
Fragments arranged by size:
2956 2120 1425 1148 766 677 330 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 9115
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9667
NheI G'CTAG_C
Cuts at: 1052 5852 1052
Size: 4800 4867
NotI GC'GGCC_GC
Cuts at: 7114 7114
Size: 9667
NruI TCG'CGA
Cuts at: 6411 6411
Size: 9667
NsiI A_TGCA'T
Cuts at: 6395 6399 6395
Size: 4 9663
NspI r_CATG'y
Cuts at: 1132 1229 2128 3386 4097 5680 6137 6397 7025
Size: 97 899 1258 711 1583 457 260 628
Cuts at: 7025 8570 1132
Size: 1545 2229
Fragments arranged by size:
2229 1583 1545 1258 899 711 628 457
260 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9667
PciI A'CATG_T
Cuts at: 8566 8566
Size: 9667
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5612 1108
Size: 4504 5163
PfoI T'CCnGG_A
Cuts at: 4771 4771
Size: 9667
PmeI GTTT'AAAC
Cuts at: 6971 6971
Size: 9667
PmlI CAC'GTG
Cuts at: 3208 3208
Size: 9667
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7736 7768 9249 9281 7736
Size: 32 1481 32 8122
Fragments arranged by size:
8122 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2546 6535 2546
Size: 3989 5678
PshAI GACnn'nnGTC
Cuts at: 1613 5070 1613
Size: 3457 6210
PsiI TTA'TAA
Cuts at: 7237 7237
Size: 9667
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5142 5174 5142
Size: 32 9635
PstI C_TGCA'G
Cuts at: 830 2174 2704 3366 5716 7019 830
Size: 1344 530 662 2350 1303 3478
Fragments arranged by size:
3478 2350 1344 1303 662 530
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9667
PvuII CAG'CTG
Cuts at: 3031 3361 6317 3031
Size: 330 2956 6381
Fragments arranged by size:
6381 2956 330
SacI G_AGCT'C
Cuts at: 2157 5367 2157
Size: 3210 6457
SalI G'TCGA_C
Cuts at: 1936 5227 7009 1936
Size: 3291 1782 4594
Fragments arranged by size:
4594 3291 1782
SanDI GG'GwC_CC
Cuts at: 2546 2546
Size: 9667
SapI GCTCTTCn'nnn_
Cuts at: 3111 3603 4440 3111
Size: 492 837 8338
Fragments arranged by size:
8338 837 492
SbfI CC_TGCA'GG
Cuts at: 7019 7019
Size: 9667
ScaI AGT'ACT
Cuts at: 7915 9627 7915
Size: 1712 7955
SfcI C'TryA_G
Cuts at: 826 1045 2170 2700 3350 3362 5712 7015 8150
Size: 219 1125 530 650 12 2350 1303 1135
Cuts at: 8150 8831 9022 826
Size: 681 191 1471
Fragments arranged by size:
2350 1471 1303 1135 1125 681 650 530
219 191 12
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9667
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9667
SmaI CCC'GGG
Cuts at: 1872 6994 1872
Size: 5122 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4817 7771 8672 8934 820
Size: 197 926 2874 2954 901 262 1553
Fragments arranged by size:
2954 2874 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 7458 493
Size: 6965 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9667
SphI G_CATG'C
Cuts at: 1132 4097 6397 7025 1132
Size: 2965 2300 628 3774
Fragments arranged by size:
3774 2965 2300 628
SspI AAT'ATT
Cuts at: 5 52 4555 5261 5777 7591 5
Size: 47 4503 706 516 1814 2081
Fragments arranged by size:
4503 2081 1814 706 516 47
StuI AGG'CCT
Cuts at: 1517 2664 1517
Size: 1147 8520
StyI C'CwwG_G
Cuts at: 513 1065 5398 5439 5564 5622 6206 7106 513
Size: 552 4333 41 125 58 584 900 3074
Fragments arranged by size:
4333 3074 900 584 552 125 58 41
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2361 6825 7694 7711 7864 8049 8494
Size: 1255 781 4464 869 17 153 185 445
Cuts at: 8494 325
Size: 1498
Fragments arranged by size:
4464 1498 1255 869 781 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3331 3586 3652
Size: 274 80 33 51 1443 1354 255 66
Cuts at: 3652 5149 5643 6607 6863 7913 9625 96
Size: 1497 494 964 256 1050 1712 138
Fragments arranged by size:
1712 1497 1443 1354 1050 964 494 274
256 255 138 80 66 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2098 2130 9627 9659 2098
Size: 32 7497 32 2106
Fragments arranged by size:
7497 2106 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9667
XbaI T'CTAG_A
Cuts at: 3283 4231 5062 6961 7003 3283
Size: 948 831 1899 42 5947
Fragments arranged by size:
5947 1899 948 831 42
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4426 5405 6173 6932 4426
Size: 979 768 759 7161
Fragments arranged by size:
7161 979 768 759
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9667
XmaI C'CCGG_G
Cuts at: 1870 6992 1870
Size: 5122 4545
XmnI GAAnn'nnTTC
Cuts at: 3057 5028 5631 6303 7796 3057
Size: 1971 603 672 1493 4928
Fragments arranged by size:
4928 1971 1493 672 603
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7475 276
Size: 53 83 186 936 5941 2468
Fragments arranged by size:
5941 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NruI NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PsrI PstI PvuI PvuII SacI SalI SanDI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI BsiWI BsmBI BstZ17I FseI FspAI KasI
NaeI NarI NgoMIV PacI PspOMI PspXI RsrII SacII
SexAI SfiI SfoI SrfI SwaI