Restriction map for
pFN21AE6892
(Circular) (Six-base) MAPSORT of: pFN21AE6892.seq Check: 4860 from: 1 to: 5788
pFN21AE6892 5788 bp
With 182 enzymes: SgfI *
April 28, 2014 11:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3598 278
Size: 53 83 186 936 2062 2468
Fragments arranged by size:
2468 2062 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3131 1937
Size: 1194 4594
Acc65I G'GTAC_C
Cuts at: 2916 3051 3109 2916
Size: 135 58 5595
Fragments arranged by size:
5595 135 58
AclI AA'CG_TT
Cuts at: 1563 3915 4288 1563
Size: 2352 373 3063
Fragments arranged by size:
3063 2352 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2980 3851 5235 5503 1632
Size: 1348 871 1384 268 1917
Fragments arranged by size:
1917 1384 1348 871 268
AfeI AGC'GCT
Cuts at: 2396 2396
Size: 5788
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5591
AflIII A'CryG_T
Cuts at: 1203 2664 4687 5664 1203
Size: 1461 2023 977 1327
Fragments arranged by size:
2023 1461 1327 977
AgeI A'CCGG_T
Cuts at: 4619 4619
Size: 5788
AhdI GACnn_n'nnGTC
Cuts at: 4517 4517
Size: 5788
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5788
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5788
AlwNI CAG_nnn'CTG
Cuts at: 2077 2440 2641 2929 3531 5103 2077
Size: 363 201 288 602 1572 2762
Fragments arranged by size:
2762 1572 602 363 288 201
ApaI G_GGCC'C
Cuts at: 2831 2831
Size: 5788
ApaLI G'TGCA_C
Cuts at: 2327 3845 5001 2327
Size: 1518 1156 3114
Fragments arranged by size:
3114 1518 1156
ApoI r'AATT_y
Cuts at: 1493 3097 3323 3475 5584 1493
Size: 1604 226 152 2109 1697
Fragments arranged by size:
2109 1697 1604 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4342 160
Size: 872 3310 1606
Fragments arranged by size:
3310 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5788
AvaI C'yCGr_G
Cuts at: 1870 1943 2115 2377 3113 1870
Size: 73 172 262 736 4545
Fragments arranged by size:
4545 736 262 172 73
AvrII C'CTAG_G
Cuts at: 2138 2138
Size: 5788
BamHI G'GATC_C
Cuts at: 2008 2132 3118 3485 2008
Size: 124 986 367 4311
Fragments arranged by size:
4311 986 367 124
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2043 2257 2916 3051 3109
Size: 325 217 627 256 214 659 135 58
Cuts at: 3109 4564 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 659 627 325 256 217 214
135 58
BanII G_rGCy'C
Cuts at: 1391 2146 2831 2900 3107 1391
Size: 755 685 69 207 4072
Fragments arranged by size:
4072 755 685 207 69
BbeI G_GCGC'C
Cuts at: 2047 2047
Size: 5788
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4817
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3979 4013 1903
Size: 34 2042 34 3678
Fragments arranged by size:
3678 2042 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3680 4890 3680
Size: 1210 4578
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5788
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2035 2389 2801
Size: 107 122 71 631 605 363 354 412
Cuts at: 2801 4399 136
Size: 1598 1525
Fragments arranged by size:
1598 1525 631 605 412 363 354 122
107 71
BglII A'GATC_T
Cuts at: 5783 5783
Size: 5788
BlpI GC'TnA_GC
Cuts at: 3205 3205
Size: 5788
Bme1580I G_kGCm'C
Cuts at: 1790 2262 2331 2817 2831 3007 3849 5005 1790
Size: 472 69 486 14 176 842 1156 2573
Fragments arranged by size:
2573 1156 842 486 472 176 69 14
BmgBI CAC'GTC
Cuts at: 1318 2665 1318
Size: 1347 4441
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2142 2344 3519
Size: 416 53 83 399 24 725 202 1175
Cuts at: 3519 4477 442
Size: 958 1753
Fragments arranged by size:
1753 1175 958 725 416 399 202 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5788
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2540 2819 4448 1239
Size: 1301 279 1629 2579
Fragments arranged by size:
2579 1629 1301 279
Bpu10I CC'TnA_GC
Cuts at: 2954 2954
Size: 5788
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2639 3913 4778 5076 2639
Size: 1274 865 298 3351
Fragments arranged by size:
3351 1274 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4451 882
Size: 617 2952 2219
Fragments arranged by size:
2952 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2881 3579 493
Size: 711 1677 698 2702
Fragments arranged by size:
2702 1677 711 698
BsaBI GATnn'nnATC
Cuts at: 5782 5782
Size: 5788
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2044 2868 3595 3977
Size: 53 83 186 936 511 824 727 382
Cuts at: 3977 4612 275
Size: 635 1451
Fragments arranged by size:
1451 936 824 727 635 511 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4221 4619 4893 5040 5451 4221
Size: 398 274 147 411 4558
Fragments arranged by size:
4558 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2513 2543 2660 2690 2702
Size: 30 165 30 1110 30 117 30 12
Cuts at: 2702 2732 1178
Size: 30 4234
Fragments arranged by size:
4234 1110 165 117 30 30 30 30
30 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5788
BseYI C'CCAG_C
Cuts at: 2824 2831 3000 4991 5487 2824
Size: 7 169 1991 496 3125
Fragments arranged by size:
3125 1991 496 169 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2040 1163
Size: 573 304 4911
Fragments arranged by size:
4911 573 304
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2293 3238 3999 5027 1540
Size: 275 186 292 945 761 1028 2301
Fragments arranged by size:
2301 1028 945 761 292 275 186
BsiHKAI G_wGCw'C
Cuts at: 2331 3849 3934 5005 2331
Size: 1518 85 1071 3114
Fragments arranged by size:
3114 1518 1071 85
BsmI GAATG_Cn'
Cuts at: 3299 3392 3299
Size: 93 5695
BsmBI CGTCTCn'nnnn_
Cuts at: 2112 2705 2969 2112
Size: 593 264 4931
Fragments arranged by size:
4931 593 264
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2146 2262 2331 2817 2831 2900 3007
Size: 399 356 116 69 486 14 69 107
Cuts at: 3007 3107 3849 3934 5005 1391
Size: 100 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 486 399 356 116 107
100 85 69 69 14
BspEI T'CCGG_A
Cuts at: 5451 5451
Size: 5788
BspHI T'CATG_A
Cuts at: 3677 5407 3677
Size: 1730 4058
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2112 2928 3143 844
Size: 1268 816 215 3489
Fragments arranged by size:
3489 1268 816 215
BsrBI CCG'CTC
Cuts at: 1394 1823 2554 3675 1394
Size: 429 731 1121 3507
Fragments arranged by size:
3507 1121 731 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4283 4457 65
Size: 1183 3035 174 1396
Fragments arranged by size:
3035 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4432 4619 1700
Size: 110 2622 187 2869
Fragments arranged by size:
2869 2622 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5788
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5788
BssSI C'ACGA_G
Cuts at: 3848 4860 3848
Size: 1012 4776
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3024 794
Size: 2230 3558
BstBI TT'CG_AA
Cuts at: 4592 4592
Size: 5788
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5788
BstXI CCAn_nnnn'nTGG
Cuts at: 2908 2908
Size: 5788
BstYI r'GATC_y
Cuts at: 1972 2008 2132 2400 3118 3485 3870 3887 5339
Size: 36 124 268 718 367 385 17 1452
Cuts at: 5339 5425 5437 5783 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 718 385 367 346 268 124
86 36 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2039 2525 2817 513
Size: 552 939 35 486 292 3484
Fragments arranged by size:
3484 939 552 486 292 35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5049
Fragments arranged by size:
5049 683 56
BtsI GCAGTG_nn'
Cuts at: 2060 2283 3304 4098 4118 2060
Size: 223 1021 794 20 3730
Fragments arranged by size:
3730 1021 794 223 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5788
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5753
DraI TTT'AAA
Cuts at: 3092 3092
Size: 5788
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4795 809
Size: 507 3479 1802
Fragments arranged by size:
3479 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2036 2055 2533
Size: 54 1425 50 275 18 206 19 478
Cuts at: 2533 2565 3235 4124 5753 8
Size: 32 670 889 1629 43
Fragments arranged by size:
1629 1425 889 670 478 275 206 54
50 43 32 19 18
EagI C'GGCC_G
Cuts at: 1537 1812 3235 1537
Size: 275 1423 4090
Fragments arranged by size:
4090 1423 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3718 1353
Size: 2365 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4375 4749 4895 387
Size: 1064 138 2786 374 146 1280
Fragments arranged by size:
2786 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2540 2819 2980 3851 4448 5235 5503
Size: 393 908 279 161 871 597 787 268
Cuts at: 5503 1239
Size: 1524
Fragments arranged by size:
1524 908 871 787 597 393 279 268
161
EcoNI CCTnn'n_nnAGG
Cuts at: 2693 2693
Size: 5788
EcoO109I rG'GnC_Cy
Cuts at: 2154 2828 2905 2154
Size: 674 77 5037
Fragments arranged by size:
5037 674 77
EcoRI G'AATT_C
Cuts at: 3097 3475 3097
Size: 378 5410
EcoRV GAT'ATC
Cuts at: 2341 2341
Size: 5788
FspI TGC'GCA
Cuts at: 3065 4294 3065
Size: 1229 4559
HaeII r_GCGC'y
Cuts at: 1678 2047 2398 4935 1678
Size: 369 351 2537 2531
Fragments arranged by size:
2537 2531 369 351
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2330 2362 2513 2545 2702
Size: 32 135 32 758 32 151 32 157
Cuts at: 2702 2734 4429 4461 4503 4535 1373
Size: 32 1695 32 42 32 2626
Fragments arranged by size:
2626 1695 758 157 151 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3132 3378 669
Size: 988 281 1194 246 3079
Fragments arranged by size:
3079 1194 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5788
HpaI GTT'AAC
Cuts at: 3378 3378
Size: 5788
KasI G'GCGC_C
Cuts at: 2043 2043
Size: 5788
KpnI G_GTAC'C
Cuts at: 2920 3055 3113 2920
Size: 135 58 5595
Fragments arranged by size:
5595 135 58
MfeI C'AATT_G
Cuts at: 3387 3387
Size: 5788
MluI A'CGCG_T
Cuts at: 5664 5664
Size: 5788
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2373 2517 2622 4902 5086 1305
Size: 1068 144 105 2280 184 2007
Fragments arranged by size:
2280 2007 1068 184 144 105
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2038 10
Size: 54 1425 343 206 3760
Fragments arranged by size:
3760 1425 343 206 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2228 2879 3746 4105 4264 518
Size: 648 1062 651 867 359 159 2042
Fragments arranged by size:
2042 1062 867 651 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2049 2074 2244 3000 3204 3881 5029 5274
Size: 443 25 170 756 204 677 1148 245
Cuts at: 5274 1606
Size: 2120
Fragments arranged by size:
2120 1148 756 677 443 245 204 170
25
NarI GG'CG_CC
Cuts at: 2044 2044
Size: 5788
NcoI C'CATG_G
Cuts at: 513 1065 2004 2039 513
Size: 552 939 35 4262
Fragments arranged by size:
4262 939 552 35
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5788
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5788
NotI GC'GGCC_GC
Cuts at: 3235 3235
Size: 5788
NspI r_CATG'y
Cuts at: 1132 1229 3146 4691 1132
Size: 97 1917 1545 2229
Fragments arranged by size:
2229 1917 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2014 1348
Size: 666 5122
PciI A'CATG_T
Cuts at: 4687 4687
Size: 5788
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2440 1108
Size: 1332 4456
PmeI GTTT'AAAC
Cuts at: 3092 3092
Size: 5788
PmlI CAC'GTG
Cuts at: 2881 2881
Size: 5788
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3857 3889 5370 5402 3857
Size: 32 1481 32 4243
Fragments arranged by size:
4243 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2154 2905 2154
Size: 751 5037
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5788
PsiI TTA'TAA
Cuts at: 3358 3358
Size: 5788
PspOMI G'GGCC_C
Cuts at: 2827 2827
Size: 5788
PstI C_TGCA'G
Cuts at: 830 2646 2925 3039 3140 830
Size: 1816 279 114 101 3478
Fragments arranged by size:
3478 1816 279 114 101
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5788
PvuII CAG'CTG
Cuts at: 2074 3000 2074
Size: 926 4862
SalI G'TCGA_C
Cuts at: 1936 3130 1936
Size: 1194 4594
SbfI CC_TGCA'GG
Cuts at: 2646 2925 3140 2646
Size: 279 215 5294
Fragments arranged by size:
5294 279 215
ScaI AGT'ACT
Cuts at: 4036 5748 4036
Size: 1712 4076
SfcI C'TryA_G
Cuts at: 826 1045 2642 2921 3035 3136 4271 4952 5143
Size: 219 1597 279 114 101 1135 681 191
Cuts at: 5143 826
Size: 1471
Fragments arranged by size:
1597 1471 1135 681 279 219 191 114
101
SfoI GGC'GCC
Cuts at: 2045 2045
Size: 5788
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5788
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5788
SmaI CCC'GGG
Cuts at: 1872 3115 1872
Size: 1243 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2654 3892 4793 5055 820
Size: 197 926 711 1238 901 262 1553
Fragments arranged by size:
1553 1238 926 901 711 262 197
SnaBI TAC'GTA
Cuts at: 493 3579 493
Size: 3086 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5788
SphI G_CATG'C
Cuts at: 1132 3146 1132
Size: 2014 3774
SspI AAT'ATT
Cuts at: 5 52 3712 5
Size: 47 3660 2081
Fragments arranged by size:
3660 2081 47
StuI AGG'CCT
Cuts at: 1517 2804 3023 1517
Size: 1287 219 4282
Fragments arranged by size:
4282 1287 219
StyI C'CwwG_G
Cuts at: 513 1065 2004 2039 2138 2694 2901 3227 513
Size: 552 939 35 99 556 207 326 3074
Fragments arranged by size:
3074 939 556 552 326 207 99 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2006 2562 2588 3815 3832 3985 4170
Size: 1255 426 556 26 1227 17 153 185
Cuts at: 4170 4615 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1227 556 445 426 185 153
26 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4034 5746 96
Size: 274 80 33 51 1443 2057 1712 138
Fragments arranged by size:
2057 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5748 5780 5748
Size: 32 5756
Tth111I GACn'n_nGTC
Cuts at: 1316 2318 2716 1316
Size: 1002 398 4388
Fragments arranged by size:
4388 1002 398
XbaI T'CTAG_A
Cuts at: 3124 3124
Size: 5788
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2037 2575 2011
Size: 26 538 5224
Fragments arranged by size:
5224 538 26
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5788
XmaI C'CCGG_G
Cuts at: 1870 3113 1870
Size: 1243 4545
XmnI GAAnn'nnTTC
Cuts at: 3917 3917
Size: 5788
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3596 276
Size: 53 83 186 936 2062 2468
Fragments arranged by size:
2468 2062 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI BaeI BbvCI BfrBI BplI BsiWI
BstZ17I Bsu36I DraIII EcoICRI FalI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PfoI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SrfI SwaI