Restriction map for
pFN21AE6819
(Circular) (Six-base) MAPSORT of: pFN21AE6819.seq Check: 1357 from: 1 to: 5731
pFN21AE6819 5731 bp
With 182 enzymes: SgfI *
June 19, 2019 18:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2002 2204 2002
Size: 202 5529
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2078 3541 278
Size: 53 83 186 936 542 1463 2468
Fragments arranged by size:
2468 1463 936 542 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2382 3074 1937
Size: 445 692 4594
Fragments arranged by size:
4594 692 445
Acc65I G'GTAC_C
Cuts at: 3052 3052
Size: 5731
AclI AA'CG_TT
Cuts at: 1563 2227 3858 4231 1563
Size: 664 1631 373 3063
Fragments arranged by size:
3063 1631 664 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2371 3794 5178 5446 1632
Size: 739 1423 1384 268 1917
Fragments arranged by size:
1917 1423 1384 739 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5534
AflIII A'CryG_T
Cuts at: 1203 4630 5607 1203
Size: 3427 977 1327
Fragments arranged by size:
3427 1327 977
AgeI A'CCGG_T
Cuts at: 4562 4562
Size: 5731
AhdI GACnn_n'nnGTC
Cuts at: 2079 4460 2079
Size: 2381 3350
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5731
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5731
AlwNI CAG_nnn'CTG
Cuts at: 2017 2495 2557 2812 3474 5046 2017
Size: 478 62 255 662 1572 2702
Fragments arranged by size:
2702 1572 662 478 255 62
ApaLI G'TGCA_C
Cuts at: 2164 3788 4944 2164
Size: 1624 1156 2951
Fragments arranged by size:
2951 1624 1156
ApoI r'AATT_y
Cuts at: 1493 2334 3040 3266 3418 5527 1493
Size: 841 706 226 152 2109 1697
Fragments arranged by size:
2109 1697 841 706 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4285 160
Size: 872 3253 1606
Fragments arranged by size:
3253 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5731
AvaI C'yCGr_G
Cuts at: 1870 1943 3056 1870
Size: 73 1113 4545
Fragments arranged by size:
4545 1113 73
BamHI G'GATC_C
Cuts at: 3061 3428 3061
Size: 367 5364
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2027 2323 3052 4507 618
Size: 325 217 627 240 296 729 1455 1842
Fragments arranged by size:
1842 1455 729 627 325 296 240 217
BanII G_rGCy'C
Cuts at: 1391 3050 1391
Size: 1659 4072
BbeI G_GCGC'C
Cuts at: 2031 2031
Size: 5731
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2794 928
Size: 971 895 3865
Fragments arranged by size:
3865 971 895
BbvCI CC'TCA_GC
Cuts at: 2063 2838 2063
Size: 775 4956
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2653 2687 3922 3956 1903
Size: 34 716 34 1235 34 3678
Fragments arranged by size:
3678 1235 716 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2446 3623 4833 2446
Size: 1177 1210 3344
Fragments arranged by size:
3344 1210 1177
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5731
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4342 136
Size: 107 122 71 631 605 2670 1525
Fragments arranged by size:
2670 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5726 5726
Size: 5731
BlpI GC'TnA_GC
Cuts at: 3148 3148
Size: 5731
Bme1580I G_kGCm'C
Cuts at: 1790 2168 3792 4948 1790
Size: 378 1624 1156 2573
Fragments arranged by size:
2573 1624 1156 378
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5731
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3462 4420 442
Size: 416 53 83 399 24 2045 958 1753
Fragments arranged by size:
2045 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5731
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2579 4391 1239
Size: 1340 1812 2579
Fragments arranged by size:
2579 1812 1340
Bpu10I CC'TnA_GC
Cuts at: 2063 2457 2838 2063
Size: 394 381 4956
Fragments arranged by size:
4956 394 381
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2313 3856 4721 5019 2313
Size: 1543 865 298 3025
Fragments arranged by size:
3025 1543 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4394 882
Size: 617 2895 2219
Fragments arranged by size:
2895 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3522 493
Size: 711 2318 2702
Fragments arranged by size:
2702 2318 711
BsaBI GATnn'nnATC
Cuts at: 5725 5725
Size: 5731
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2028 2075 3538 3920
Size: 53 83 186 936 495 47 1463 382
Cuts at: 3920 4555 275
Size: 635 1451
Fragments arranged by size:
1463 1451 936 635 495 382 186 83
53 47
BsaWI w'CCGG_w
Cuts at: 2078 4164 4562 4836 4983 5394 2078
Size: 2086 398 274 147 411 2415
Fragments arranged by size:
2415 2086 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2185 2215 1178
Size: 30 165 30 782 30 4694
Fragments arranged by size:
4694 782 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2080 2193 2566 1186
Size: 894 113 373 4351
Fragments arranged by size:
4351 894 373 113
BseYI C'CCAG_C
Cuts at: 2636 4934 5430 2636
Size: 2298 496 2937
Fragments arranged by size:
2937 2298 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2182 2394 1163
Size: 573 446 212 4500
Fragments arranged by size:
4500 573 446 212
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3181 3942 4970 1540
Size: 275 186 1180 761 1028 2301
Fragments arranged by size:
2301 1180 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2168 2185 3792 3877 4948 2168
Size: 17 1607 85 1071 2951
Fragments arranged by size:
2951 1607 1071 85 17
BsmI GAATG_Cn'
Cuts at: 2546 3242 3335 2546
Size: 696 93 4942
Fragments arranged by size:
4942 696 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2680 2680
Size: 5731
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2168 2185 3050 3792 3877 4948 1391
Size: 399 378 17 865 742 85 1071 2174
Fragments arranged by size:
2174 1071 865 742 399 378 85 17
BspEI T'CCGG_A
Cuts at: 5394 5394
Size: 5731
BspHI T'CATG_A
Cuts at: 3620 5350 3620
Size: 1730 4001
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2002 2204 3086 844
Size: 1158 202 882 3489
Fragments arranged by size:
3489 1158 882 202
BsrBI CCG'CTC
Cuts at: 1394 1823 3618 1394
Size: 429 1795 3507
Fragments arranged by size:
3507 1795 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4226 4400 65
Size: 1183 2978 174 1396
Fragments arranged by size:
2978 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4375 4562 1700
Size: 110 2565 187 2869
Fragments arranged by size:
2869 2565 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5731
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5731
BssSI C'ACGA_G
Cuts at: 2729 3791 4803 2729
Size: 1062 1012 3657
Fragments arranged by size:
3657 1062 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5731
BstBI TT'CG_AA
Cuts at: 4535 4535
Size: 5731
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5731
BstYI r'GATC_y
Cuts at: 1972 3061 3428 3813 3830 5282 5368 5380 5726
Size: 1089 367 385 17 1452 86 12 346
Cuts at: 5726 1972
Size: 1977
Fragments arranged by size:
1977 1452 1089 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4240
Fragments arranged by size:
4240 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4992
Fragments arranged by size:
4992 683 56
BtsI GCAGTG_nn'
Cuts at: 3247 4041 4061 3247
Size: 794 20 4917
Fragments arranged by size:
4917 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5731
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5696
DraI TTT'AAA
Cuts at: 3018 3035 3018
Size: 17 5714
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4738 809
Size: 507 3422 1802
Fragments arranged by size:
3422 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2908 3178 4067
Size: 54 1425 50 275 18 1078 270 889
Cuts at: 4067 5696 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1078 889 275 270 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3178 1537
Size: 275 1366 4090
Fragments arranged by size:
4090 1366 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3661 1353
Size: 2308 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4318 4692 4838 387
Size: 1064 138 2729 374 146 1280
Fragments arranged by size:
2729 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2371 2579 3794 4391 5178 5446 1239
Size: 393 739 208 1215 597 787 268 1524
Fragments arranged by size:
1524 1215 787 739 597 393 268 208
EcoNI CCTnn'n_nnAGG
Cuts at: 2283 2283
Size: 5731
EcoRI G'AATT_C
Cuts at: 3040 3418 3040
Size: 378 5353
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2374 2374
Size: 5731
FspI TGC'GCA
Cuts at: 4237 4237
Size: 5731
HaeII r_GCGC'y
Cuts at: 1678 2031 4878 1678
Size: 353 2847 2531
Fragments arranged by size:
2847 2531 353
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2065 2097 2908 2940 4372
Size: 32 135 32 493 32 811 32 1432
Cuts at: 4372 4404 4446 4478 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1432 811 493 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3075 3321 669
Size: 988 281 1137 246 3079
Fragments arranged by size:
3079 1137 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2044 3012 748
Size: 1296 968 3467
Fragments arranged by size:
3467 1296 968
HpaI GTT'AAC
Cuts at: 3321 3321
Size: 5731
KasI G'GCGC_C
Cuts at: 2027 2027
Size: 5731
KpnI G_GTAC'C
Cuts at: 3056 3056
Size: 5731
MfeI C'AATT_G
Cuts at: 2844 3330 2844
Size: 486 5245
MluI A'CGCG_T
Cuts at: 5607 5607
Size: 5731
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2994 4845 5029 1305
Size: 1689 1851 184 2007
Fragments arranged by size:
2007 1851 1689 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2910 10
Size: 54 1425 343 1078 2831
Fragments arranged by size:
2831 1425 1078 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3689 4048 4207 518
Size: 648 2523 359 159 2042
Fragments arranged by size:
2523 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2171 2812 3147 3824 4972 5217 1606
Size: 565 641 335 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 641 565 335 245
NarI GG'CG_CC
Cuts at: 2028 2028
Size: 5731
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4240
Fragments arranged by size:
4240 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5731
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5731
NotI GC'GGCC_GC
Cuts at: 3178 3178
Size: 5731
NspI r_CATG'y
Cuts at: 1132 1229 2946 3089 4634 1132
Size: 97 1717 143 1545 2229
Fragments arranged by size:
2229 1717 1545 143 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5731
PciI A'CATG_T
Cuts at: 4630 4630
Size: 5731
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2812 1108
Size: 1704 4027
PmeI GTTT'AAAC
Cuts at: 3035 3035
Size: 5731
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3800 3832 5313 5345 3800
Size: 32 1481 32 4186
Fragments arranged by size:
4186 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5731
PsiI TTA'TAA
Cuts at: 3301 3301
Size: 5731
PstI C_TGCA'G
Cuts at: 830 2147 2508 3083 830
Size: 1317 361 575 3478
Fragments arranged by size:
3478 1317 575 361
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5731
PvuII CAG'CTG
Cuts at: 2171 2812 2171
Size: 641 5090
SalI G'TCGA_C
Cuts at: 1936 3073 1936
Size: 1137 4594
SbfI CC_TGCA'GG
Cuts at: 3083 3083
Size: 5731
ScaI AGT'ACT
Cuts at: 3979 5691 3979
Size: 1712 4019
SfcI C'TryA_G
Cuts at: 826 1045 2136 2143 2504 3079 4214 4895 5086
Size: 219 1091 7 361 575 1135 681 191
Cuts at: 5086 826
Size: 1471
Fragments arranged by size:
1471 1135 1091 681 575 361 219 191
7
SfoI GGC'GCC
Cuts at: 2029 2029
Size: 5731
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5731
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5731
SmaI CCC'GGG
Cuts at: 1872 3058 1872
Size: 1186 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2328 3835 4736 4998 820
Size: 197 926 385 1507 901 262 1553
Fragments arranged by size:
1553 1507 926 901 385 262 197
SnaBI TAC'GTA
Cuts at: 493 3522 493
Size: 3029 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5731
SphI G_CATG'C
Cuts at: 1132 3089 1132
Size: 1957 3774
SspI AAT'ATT
Cuts at: 5 52 3655 5
Size: 47 3603 2081
Fragments arranged by size:
3603 2081 47
StuI AGG'CCT
Cuts at: 1517 2266 1517
Size: 749 4982
StyI C'CwwG_G
Cuts at: 513 1065 2004 2630 3170 513
Size: 552 939 626 540 3074
Fragments arranged by size:
3074 939 626 552 540
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2168 3758 3775 3928 4113 4558 325
Size: 1255 588 1590 17 153 185 445 1498
Fragments arranged by size:
1590 1498 1255 588 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2187 2643 3977
Size: 274 80 33 51 1443 210 456 1334
Cuts at: 3977 5689 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1334 456 274 210 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2185 2217 2641 2673 5691 5723 2185
Size: 32 424 32 3018 32 2193
Fragments arranged by size:
3018 2193 424 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5731
XbaI T'CTAG_A
Cuts at: 3067 3067
Size: 5731
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5731
XmaI C'CCGG_G
Cuts at: 1870 3056 1870
Size: 1186 4545
XmnI GAAnn'nnTTC
Cuts at: 2338 3860 2338
Size: 1522 4209
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2076 3539 276
Size: 53 83 186 936 542 1463 2468
Fragments arranged by size:
2468 1463 936 542 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI
BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SrfI SwaI XcmI