Restriction map for
pFN21AE6789
(Circular) (Six-base) MAPSORT of: pFN21AE6789.seq Check: 3967 from: 1 to: 6445
pFN21AE6789 6445 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4255 278
Size: 53 83 186 936 2719 2468
Fragments arranged by size:
2719 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3788 1937
Size: 1851 4594
Acc65I G'GTAC_C
Cuts at: 2730 3766 2730
Size: 1036 5409
AclI AA'CG_TT
Cuts at: 1563 4572 4945 1563
Size: 3009 373 3063
Fragments arranged by size:
3063 3009 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2684 3569 4508 5892 6160 1632
Size: 1052 885 939 1384 268 1917
Fragments arranged by size:
1917 1384 1052 939 885 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6248
AflIII A'CryG_T
Cuts at: 1203 5344 6321 1203
Size: 4141 977 1327
Fragments arranged by size:
4141 1327 977
AgeI A'CCGG_T
Cuts at: 5276 5276
Size: 6445
AhdI GACnn_n'nnGTC
Cuts at: 5174 5174
Size: 6445
AleI CACnn'nnGTG
Cuts at: 1166 3356 3605 1166
Size: 2190 249 4006
Fragments arranged by size:
4006 2190 249
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6445
AlwNI CAG_nnn'CTG
Cuts at: 2182 4188 5760 2182
Size: 2006 1572 2867
Fragments arranged by size:
2867 2006 1572
ApaI G_GGCC'C
Cuts at: 2762 3046 2762
Size: 284 6161
ApaLI G'TGCA_C
Cuts at: 2209 2604 4502 5658 2209
Size: 395 1898 1156 2996
Fragments arranged by size:
2996 1898 1156 395
ApoI r'AATT_y
Cuts at: 1493 3754 3980 4132 6241 1493
Size: 2261 226 152 2109 1697
Fragments arranged by size:
2261 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4999 160
Size: 872 3967 1606
Fragments arranged by size:
3967 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6445
AvaI C'yCGr_G
Cuts at: 1870 1943 3323 3770 1870
Size: 73 1380 447 4545
Fragments arranged by size:
4545 1380 447 73
AvrII C'CTAG_G
Cuts at: 2854 2854
Size: 6445
BamHI G'GATC_C
Cuts at: 3775 4142 3775
Size: 367 6078
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2730 3471 3491 3766 5221
Size: 325 217 627 943 741 20 275 1455
Cuts at: 5221 618
Size: 1842
Fragments arranged by size:
1842 1455 943 741 627 325 275 217
20
BanII G_rGCy'C
Cuts at: 1391 2330 2762 3046 3096 3764 1391
Size: 939 432 284 50 668 4072
Fragments arranged by size:
4072 939 668 432 284 50
BbeI G_GCGC'C
Cuts at: 3495 3495
Size: 6445
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2672 2960 928
Size: 971 773 288 4413
Fragments arranged by size:
4413 971 773 288
BbvCI CC'TCA_GC
Cuts at: 2117 2274 2117
Size: 157 6288
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2155 2189 4636 4670 1903
Size: 34 50 34 134 34 2447 34 3678
Fragments arranged by size:
3678 2447 134 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3287 4337 5547 3287
Size: 1050 1210 4185
Fragments arranged by size:
4185 1210 1050
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6445
BfrBI ATG'CAT
Cuts at: 3037 3037
Size: 6445
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2567 3500 5056
Size: 107 122 71 631 605 895 933 1556
Cuts at: 5056 136
Size: 1525
Fragments arranged by size:
1556 1525 933 895 631 605 122 107
71
BglII A'GATC_T
Cuts at: 6440 6440
Size: 6445
BlpI GC'TnA_GC
Cuts at: 2630 3264 3862 2630
Size: 634 598 5213
Fragments arranged by size:
5213 634 598
Bme1580I G_kGCm'C
Cuts at: 1790 2213 2608 2659 2762 3046 4506 5662 1790
Size: 423 395 51 103 284 1460 1156 2573
Fragments arranged by size:
2573 1460 1156 423 395 284 103 51
BmgBI CAC'GTC
Cuts at: 1318 2458 2503 1318
Size: 1140 45 5260
Fragments arranged by size:
5260 1140 45
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4176 5134 442
Size: 416 53 83 399 24 2759 958 1753
Fragments arranged by size:
2759 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6445
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2120 2993 3152 5105 1239
Size: 881 873 159 1953 2579
Fragments arranged by size:
2579 1953 881 873 159
Bpu10I CC'TnA_GC
Cuts at: 2117 2274 3219 2117
Size: 157 945 5343
Fragments arranged by size:
5343 945 157
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4570 5435 5733 4570
Size: 865 298 5282
Fragments arranged by size:
5282 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5108 882
Size: 617 3609 2219
Fragments arranged by size:
3609 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3607 4236 493
Size: 711 2403 629 2702
Fragments arranged by size:
2702 2403 711 629
BsaBI GATnn'nnATC
Cuts at: 6439 6439
Size: 6445
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3479 3492 4252 4634
Size: 53 83 186 936 1946 13 760 382
Cuts at: 4634 5269 275
Size: 635 1451
Fragments arranged by size:
1946 1451 936 760 635 382 186 83
53 13
BsaWI w'CCGG_w
Cuts at: 4878 5276 5550 5697 6108 4878
Size: 398 274 147 411 5215
Fragments arranged by size:
5215 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2312 2342 3455 3485 3551
Size: 30 165 30 909 30 1113 30 66
Cuts at: 3551 3581 1178
Size: 30 4042
Fragments arranged by size:
4042 1113 909 165 66 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2117 2120 2291 2876 1186
Size: 931 3 171 585 4755
Fragments arranged by size:
4755 931 585 171 3
BseYI C'CCAG_C
Cuts at: 2143 2592 3045 5648 6144 2143
Size: 449 453 2603 496 2444
Fragments arranged by size:
2603 2444 496 453 449
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2768 1163
Size: 573 1032 4840
Fragments arranged by size:
4840 1032 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3895 4656 5684 1540
Size: 275 186 1894 761 1028 2301
Fragments arranged by size:
2301 1894 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2213 2608 3096 4506 4591 5662 2213
Size: 395 488 1410 85 1071 2996
Fragments arranged by size:
2996 1410 1071 488 395 85
BsmI GAATG_Cn'
Cuts at: 3956 4049 3956
Size: 93 6352
BsmBI CGTCTCn'nnnn_
Cuts at: 2785 2785
Size: 6445
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2213 2330 2608 2659 2762 3046 3096
Size: 399 423 117 278 51 103 284 50
Cuts at: 3096 3764 4506 4591 5662 1391
Size: 668 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 668 423 399 284 278
117 103 85 51 50
BspEI T'CCGG_A
Cuts at: 6108 6108
Size: 6445
BspHI T'CATG_A
Cuts at: 2509 2814 4334 6064 2509
Size: 305 1520 1730 2890
Fragments arranged by size:
2890 1730 1520 305
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2526 3800 844
Size: 1682 1274 3489
Fragments arranged by size:
3489 1682 1274
BsrBI CCG'CTC
Cuts at: 1394 1823 3223 4332 1394
Size: 429 1400 1109 3507
Fragments arranged by size:
3507 1400 1109 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2794 3019 4940 5114 65
Size: 1183 1546 225 1921 174 1396
Fragments arranged by size:
1921 1546 1396 1183 225 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2440 2676 3405 5089 5276 1700
Size: 110 630 236 729 1684 187 2869
Fragments arranged by size:
2869 1684 729 630 236 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6445
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6445
BssSI C'ACGA_G
Cuts at: 2564 4505 5517 2564
Size: 1941 1012 3492
Fragments arranged by size:
3492 1941 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6445
BstBI TT'CG_AA
Cuts at: 5249 5249
Size: 6445
BstEII G'GTnAC_C
Cuts at: 1184 3052 1184
Size: 1868 4577
BstXI CCAn_nnnn'nTGG
Cuts at: 3673 3673
Size: 6445
BstYI r'GATC_y
Cuts at: 1972 2178 2793 3525 3702 3775 4142 4527 4544
Size: 206 615 732 177 73 367 385 17
Cuts at: 4544 5996 6082 6094 6440 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 732 615 385 367 346 206
177 86 73 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2293 2367 3073 3299 3416 3530 513
Size: 552 1228 74 706 226 117 114 3428
Fragments arranged by size:
3428 1228 706 552 226 117 114 74
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2452 487
Size: 683 56 1226 4480
Fragments arranged by size:
4480 1226 683 56
BtsI GCAGTG_nn'
Cuts at: 2171 3961 4755 4775 2171
Size: 1790 794 20 3841
Fragments arranged by size:
3841 1790 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6445
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6410
DraI TTT'AAA
Cuts at: 3749 3749
Size: 6445
DraIII CAC_nnn'GTG
Cuts at: 2206 2206
Size: 6445
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5452 809
Size: 507 4136 1802
Fragments arranged by size:
4136 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3144 3407 3892
Size: 54 1425 50 275 18 1314 263 485
Cuts at: 3892 4781 6410 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1314 889 485 275 263 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3892 1537
Size: 275 2080 4090
Fragments arranged by size:
4090 2080 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2534 2582 3068 3101 3338 4375 1353
Size: 1181 48 486 33 237 1037 3423
Fragments arranged by size:
3423 1181 1037 486 237 48 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3199 5032 5406 5552 387
Size: 1064 138 1610 1833 374 146 1280
Fragments arranged by size:
1833 1610 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3094 3094
Size: 6445
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2120 2684 2993 3152 3569 4508 5105
Size: 393 488 564 309 159 417 939 597
Cuts at: 5105 5892 6160 1239
Size: 787 268 1524
Fragments arranged by size:
1524 939 787 597 564 488 417 393
309 268 159
EcoNI CCTnn'n_nnAGG
Cuts at: 2186 2858 2186
Size: 672 5773
EcoO109I rG'GnC_Cy
Cuts at: 2758 3042 3295 3713 2758
Size: 284 253 418 5490
Fragments arranged by size:
5490 418 284 253
EcoRI G'AATT_C
Cuts at: 3754 4132 3754
Size: 378 6067
FseI GG_CCGG'CC
Cuts at: 3409 3409
Size: 6445
FspI TGC'GCA
Cuts at: 4951 4951
Size: 6445
HaeII r_GCGC'y
Cuts at: 1678 2338 3495 5592 1678
Size: 660 1157 2097 2531
Fragments arranged by size:
2531 2097 1157 660
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3353 3385 5086 5118 5160
Size: 32 135 32 1781 32 1701 32 42
Cuts at: 5160 5192 1373
Size: 32 2626
Fragments arranged by size:
2626 1781 1701 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3789 4035 669
Size: 988 281 1851 246 3079
Fragments arranged by size:
3079 1851 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6445
HpaI GTT'AAC
Cuts at: 4035 4035
Size: 6445
KasI G'GCGC_C
Cuts at: 3491 3491
Size: 6445
KpnI G_GTAC'C
Cuts at: 2734 3770 2734
Size: 1036 5409
MfeI C'AATT_G
Cuts at: 4044 4044
Size: 6445
MluI A'CGCG_T
Cuts at: 6321 6321
Size: 6445
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2289 5559 5743 1305
Size: 984 3270 184 2007
Fragments arranged by size:
3270 2007 984 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3146 10
Size: 54 1425 343 1314 3309
Fragments arranged by size:
3309 1425 1314 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2201 3356 3605 4403 4762 4921 518
Size: 648 1035 1155 249 798 359 159 2042
Fragments arranged by size:
2042 1155 1035 798 648 359 249 159
MspA1I CmG'CkG
Cuts at: 1606 2054 2662 3049 3687 3861 4538 5686 5931
Size: 448 608 387 638 174 677 1148 245
Cuts at: 5931 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 638 608 448 387 245
174
NaeI GCC'GGC
Cuts at: 2678 3407 2678
Size: 729 5716
NarI GG'CG_CC
Cuts at: 3492 3492
Size: 6445
NcoI C'CATG_G
Cuts at: 513 1065 2293 3530 513
Size: 552 1228 1237 3428
Fragments arranged by size:
3428 1237 1228 552
NdeI CA'TA_TG
Cuts at: 387 3103 387
Size: 2716 3729
NgoMIV G'CCGG_C
Cuts at: 2676 3405 2676
Size: 729 5716
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6445
NotI GC'GGCC_GC
Cuts at: 3892 3892
Size: 6445
NsiI A_TGCA'T
Cuts at: 3039 3039
Size: 6445
NspI r_CATG'y
Cuts at: 1132 1229 2072 2090 3037 3803 5348 1132
Size: 97 843 18 947 766 1545 2229
Fragments arranged by size:
2229 1545 947 843 766 97 18
PasI CC'CwG_GG
Cuts at: 1348 2671 2755 3228 1348
Size: 1323 84 473 4565
Fragments arranged by size:
4565 1323 473 84
PciI A'CATG_T
Cuts at: 5344 5344
Size: 6445
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6445
PmeI GTTT'AAAC
Cuts at: 3749 3749
Size: 6445
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4514 4546 6027 6059 4514
Size: 32 1481 32 4900
Fragments arranged by size:
4900 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3295 3713 3295
Size: 418 6027
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6445
PsiI TTA'TAA
Cuts at: 4015 4015
Size: 6445
PspOMI G'GGCC_C
Cuts at: 2758 3042 2758
Size: 284 6161
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3527 3559 3527
Size: 32 6413
PstI C_TGCA'G
Cuts at: 830 2178 3585 3630 3797 830
Size: 1348 1407 45 167 3478
Fragments arranged by size:
3478 1407 1348 167 45
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6445
PvuII CAG'CTG
Cuts at: 2662 3049 2662
Size: 387 6058
SacI G_AGCT'C
Cuts at: 3096 3096
Size: 6445
SalI G'TCGA_C
Cuts at: 1936 3787 1936
Size: 1851 4594
SanDI GG'GwC_CC
Cuts at: 3713 3713
Size: 6445
SapI GCTCTTCn'nnn_
Cuts at: 2582 3101 3338 2582
Size: 519 237 5689
Fragments arranged by size:
5689 519 237
SbfI CC_TGCA'GG
Cuts at: 3797 3797
Size: 6445
ScaI AGT'ACT
Cuts at: 2390 4693 6405 2390
Size: 2303 1712 2430
Fragments arranged by size:
2430 2303 1712
SexAI A'CCwGG_T
Cuts at: 2726 3672 2726
Size: 946 5499
SfcI C'TryA_G
Cuts at: 826 1045 2174 3581 3626 3793 4928 5609 5800
Size: 219 1129 1407 45 167 1135 681 191
Cuts at: 5800 826
Size: 1471
Fragments arranged by size:
1471 1407 1135 1129 681 219 191 167
45
SfoI GGC'GCC
Cuts at: 3493 3493
Size: 6445
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6445
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6445
SmaI CCC'GGG
Cuts at: 1872 3772 1872
Size: 1900 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4549 5450 5712 820
Size: 197 926 2606 901 262 1553
Fragments arranged by size:
2606 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4236 493
Size: 3743 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6445
SphI G_CATG'C
Cuts at: 1132 2090 3037 3803 1132
Size: 958 947 766 3774
Fragments arranged by size:
3774 958 947 766
SspI AAT'ATT
Cuts at: 5 52 4369 5
Size: 47 4317 2081
Fragments arranged by size:
4317 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6445
StyI C'CwwG_G
Cuts at: 513 1065 2293 2854 3354 3530 3884 513
Size: 552 1228 561 500 176 354 3074
Fragments arranged by size:
3074 1228 561 552 500 354 176
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3263 4472 4489 4642 4827 5272 325
Size: 1255 1683 1209 17 153 185 445 1498
Fragments arranged by size:
1683 1498 1255 1209 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2388 2865 4691
Size: 274 80 33 51 1443 411 477 1826
Cuts at: 4691 6403 96
Size: 1712 138
Fragments arranged by size:
1826 1712 1443 477 411 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2163 2195 3455 3487 6405 6437 2163
Size: 32 1260 32 2918 32 2171
Fragments arranged by size:
2918 2171 1260 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2965 1316
Size: 1649 4796
XbaI T'CTAG_A
Cuts at: 3781 3781
Size: 6445
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3391 3391
Size: 6445
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6445
XmaI C'CCGG_G
Cuts at: 1870 3770 1870
Size: 1900 4545
XmnI GAAnn'nnTTC
Cuts at: 4574 4574
Size: 6445
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4253 276
Size: 53 83 186 936 2719 2468
Fragments arranged by size:
2719 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI
PvuII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BplI BsiWI BstZ17I
Bsu36I EcoRV FalI FspAI NruI PacI PfoI PmlI
PspXI RsrII SacII SfiI SrfI SwaI