Restriction map for
pFN21AE6783
(Circular) (Six-base) MAPSORT of: pFN21AE6783.seq Check: 5533 from: 1 to: 6535
pFN21AE6783 6535 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2139 2197 2139
Size: 58 6477
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4345 278
Size: 53 83 186 936 2809 2468
Fragments arranged by size:
2809 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3878 1937
Size: 1941 4594
Acc65I G'GTAC_C
Cuts at: 3856 3856
Size: 6535
AclI AA'CG_TT
Cuts at: 1563 4662 5035 1563
Size: 3099 373 3063
Fragments arranged by size:
3099 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3578 3598 4598 5982 6250 1632
Size: 1946 20 1000 1384 268 1917
Fragments arranged by size:
1946 1917 1384 1000 268 20
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6338
AflIII A'CryG_T
Cuts at: 1203 5434 6411 1203
Size: 4231 977 1327
Fragments arranged by size:
4231 1327 977
AgeI A'CCGG_T
Cuts at: 5366 5366
Size: 6535
AhdI GACnn_n'nnGTC
Cuts at: 5264 5264
Size: 6535
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6535
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6535
AlwNI CAG_nnn'CTG
Cuts at: 2068 2212 2446 2886 4278 5850 2068
Size: 144 234 440 1392 1572 2753
Fragments arranged by size:
2753 1572 1392 440 234 144
ApaI G_GGCC'C
Cuts at: 2657 2657
Size: 6535
ApaLI G'TGCA_C
Cuts at: 2311 2733 4592 5748 2311
Size: 422 1859 1156 3098
Fragments arranged by size:
3098 1859 1156 422
ApoI r'AATT_y
Cuts at: 1493 2384 2454 2528 2566 2936 3475 3844 4070
Size: 891 70 74 38 370 539 369 226
Cuts at: 4070 4222 6331 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 891 539 370 369 226 152
74 70 38
AseI AT'TA_AT
Cuts at: 160 1032 5089 160
Size: 872 4057 1606
Fragments arranged by size:
4057 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6535
AvaI C'yCGr_G
Cuts at: 1870 1943 3860 1870
Size: 73 1917 4545
Fragments arranged by size:
4545 1917 73
BamHI G'GATC_C
Cuts at: 2767 3865 4232 2767
Size: 1098 367 5070
Fragments arranged by size:
5070 1098 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2234 2824 3497 3856 5311
Size: 325 217 627 447 590 673 359 1455
Cuts at: 5311 618
Size: 1842
Fragments arranged by size:
1842 1455 673 627 590 447 359 325
217
BanII G_rGCy'C
Cuts at: 1391 2657 3357 3854 1391
Size: 1266 700 497 4072
Fragments arranged by size:
4072 1266 700 497
BbeI G_GCGC'C
Cuts at: 2238 2238
Size: 6535
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3053 3273 3333 3604 928
Size: 971 1154 220 60 271 3859
Fragments arranged by size:
3859 1154 971 271 220 60
BbvCI CC'TCA_GC
Cuts at: 3638 3638
Size: 6535
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4726 4760 1903
Size: 34 2789 34 3678
Fragments arranged by size:
3678 2789 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4427 5637 4427
Size: 1210 5325
BclI T'GATC_A
Cuts at: 1548 3237 1548
Size: 1689 4846
BfrBI ATG'CAT
Cuts at: 2982 3755 2982
Size: 773 5762
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5146 136
Size: 107 122 71 631 605 3474 1525
Fragments arranged by size:
3474 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2020 6530 2020
Size: 4510 2025
BlpI GC'TnA_GC
Cuts at: 2447 3369 3633 3952 2447
Size: 922 264 319 5030
Fragments arranged by size:
5030 922 319 264
Bme1580I G_kGCm'C
Cuts at: 1790 2272 2315 2657 2737 3206 4596 5752 1790
Size: 482 43 342 80 469 1390 1156 2573
Fragments arranged by size:
2573 1390 1156 482 469 342 80 43
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6535
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2179 2266 2823
Size: 416 53 83 399 24 762 87 557
Cuts at: 2823 3586 4266 5224 442
Size: 763 680 958 1753
Fragments arranged by size:
1753 958 763 762 680 557 416 399
87 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6535
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2441 2441
Size: 6535
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2045 2373 2415 2865 2946 3114 3758 5195
Size: 806 328 42 450 81 168 644 1437
Cuts at: 5195 1239
Size: 2579
Fragments arranged by size:
2579 1437 806 644 450 328 168 81
42
Bpu10I CC'TnA_GC
Cuts at: 3638 3638
Size: 6535
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2018 3396 4660 5525 5823 2018
Size: 1378 1264 865 298 2730
Fragments arranged by size:
2730 1378 1264 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2070 3125 3190 5198 882
Size: 617 571 1055 65 2008 2219
Fragments arranged by size:
2219 2008 1055 617 571 65
BsaAI yAC'GTr
Cuts at: 493 1204 2593 3255 4326 493
Size: 711 1389 662 1071 2702
Fragments arranged by size:
2702 1389 1071 711 662
BsaBI GATnn'nnATC
Cuts at: 6529 6529
Size: 6535
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2235 4342 4724 5359
Size: 53 83 186 936 702 2107 382 635
Cuts at: 5359 275
Size: 1451
Fragments arranged by size:
2107 1451 936 702 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2142 4968 5366 5640 5787 6198 2142
Size: 2826 398 274 147 411 2479
Fragments arranged by size:
2826 2479 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2464 2494 2793 2823 3056
Size: 30 165 30 1061 30 299 30 233
Cuts at: 3056 3086 1178
Size: 30 4627
Fragments arranged by size:
4627 1061 299 233 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2114 2117 1186
Size: 928 3 5604
Fragments arranged by size:
5604 928 3
BseYI C'CCAG_C
Cuts at: 2212 3159 3628 5738 6234 2212
Size: 947 469 2110 496 2513
Fragments arranged by size:
2513 2110 947 496 469
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2210 2521 3815 1163
Size: 573 474 311 1294 3883
Fragments arranged by size:
3883 1294 573 474 311
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3697 3985 4746 5774 1540
Size: 275 186 1696 288 761 1028 2301
Fragments arranged by size:
2301 1696 1028 761 288 275 186
BsiHKAI G_wGCw'C
Cuts at: 2032 2178 2315 2737 3357 4596 4681 5752 2032
Size: 146 137 422 620 1239 85 1071 2815
Fragments arranged by size:
2815 1239 1071 620 422 146 137 85
BsmI GAATG_Cn'
Cuts at: 4046 4139 4046
Size: 93 6442
BsmBI CGTCTCn'nnnn_
Cuts at: 2677 2677
Size: 6535
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2032 2178 2272 2315 2657 2737 3206
Size: 399 242 146 94 43 342 80 469
Cuts at: 3206 3357 3854 4596 4681 5752 1391
Size: 151 497 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 497 469 399 342 242
151 146 94 85 80 43
BspEI T'CCGG_A
Cuts at: 6198 6198
Size: 6535
BspHI T'CATG_A
Cuts at: 4424 6154 4424
Size: 1730 4805
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2139 2197 2508 3172 3890 844
Size: 1295 58 311 664 718 3489
Fragments arranged by size:
3489 1295 718 664 311 58
BsrBI CCG'CTC
Cuts at: 1394 1823 3301 4422 1394
Size: 429 1478 1121 3507
Fragments arranged by size:
3507 1478 1121 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5030 5204 65
Size: 1183 3782 174 1396
Fragments arranged by size:
3782 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5179 5366 1700
Size: 110 3369 187 2869
Fragments arranged by size:
3369 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2888 96
Size: 2792 3743
BssHII G'CGCG_C
Cuts at: 1925 2195 1925
Size: 270 6265
BssSI C'ACGA_G
Cuts at: 4595 5607 4595
Size: 1012 5523
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2319 2428 794
Size: 1525 109 4901
Fragments arranged by size:
4901 1525 109
BstBI TT'CG_AA
Cuts at: 5339 5339
Size: 6535
BstEII G'GTnAC_C
Cuts at: 1184 3166 1184
Size: 1982 4553
BstYI r'GATC_y
Cuts at: 1972 2020 2767 3068 3865 4232 4617 4634 6086
Size: 48 747 301 797 367 385 17 1452
Cuts at: 6086 6172 6184 6530 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 797 747 385 367 346 301
86 48 17 12
Bsu36I CC'TnA_GG
Cuts at: 2288 3572 2288
Size: 1284 5251
BtgI C'CryG_G
Cuts at: 513 1065 2004 2559 2649 3111 513
Size: 552 939 555 90 462 3937
Fragments arranged by size:
3937 939 555 552 462 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5796
Fragments arranged by size:
5796 683 56
BtsI GCAGTG_nn'
Cuts at: 2669 2700 4051 4845 4865 2669
Size: 31 1351 794 20 4339
Fragments arranged by size:
4339 1351 794 31 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6535
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6500
DraI TTT'AAA
Cuts at: 3839 3839
Size: 6535
DraIII CAC_nnn'GTG
Cuts at: 2263 2319 2263
Size: 56 6479
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2336 2705 5542 809
Size: 507 1020 369 2837 1802
Fragments arranged by size:
2837 1802 1020 507 369
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3249 3488 3982
Size: 54 1425 50 275 18 1419 239 494
Cuts at: 3982 4871 6500 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1419 889 494 275 239 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3982 1537
Size: 275 2170 4090
Fragments arranged by size:
4090 2170 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4465 1353
Size: 3112 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3329 5122 5496 5642 387
Size: 1064 138 1740 1793 374 146 1280
Fragments arranged by size:
1793 1740 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3355 3355
Size: 6535
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2045 2373 2415 2865 2946 3114 3578
Size: 393 413 328 42 450 81 168 464
Cuts at: 3578 3598 3758 4598 5195 5982 6250 1239
Size: 20 160 840 597 787 268 1524
Fragments arranged by size:
1524 840 787 597 464 450 413 393
328 268 168 160 81 42 20
EcoO109I rG'GnC_Cy
Cuts at: 2065 2295 2654 3676 2065
Size: 230 359 1022 4924
Fragments arranged by size:
4924 1022 359 230
EcoRI G'AATT_C
Cuts at: 3844 4222 3844
Size: 378 6157
FspI TGC'GCA
Cuts at: 5041 5041
Size: 6535
HaeII r_GCGC'y
Cuts at: 1678 2238 5682 1678
Size: 560 3444 2531
Fragments arranged by size:
3444 2531 560
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3056 3088 3222 3229 3254
Size: 32 135 32 1484 32 134 7 25
Cuts at: 3254 3261 3416 3448 3743 3775 5176 5208 5250
Size: 7 155 32 295 32 1401 32 42
Cuts at: 5250 5282 1373
Size: 32 2626
Fragments arranged by size:
2626 1484 1401 295 155 135 134 42
32 32 32 32 32 32 32 25
7 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2710 3446 3660 3879 4125 669
Size: 988 281 772 736 214 219 246 3079
Fragments arranged by size:
3079 988 772 736 281 246 219 214
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6535
HpaI GTT'AAC
Cuts at: 3660 4125 3660
Size: 465 6070
KasI G'GCGC_C
Cuts at: 2234 2234
Size: 6535
KpnI G_GTAC'C
Cuts at: 3860 3860
Size: 6535
MfeI C'AATT_G
Cuts at: 4134 4134
Size: 6535
MluI A'CGCG_T
Cuts at: 6411 6411
Size: 6535
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2395 2414 5649 5833 1305
Size: 1090 19 3235 184 2007
Fragments arranged by size:
3235 2007 1090 184 19
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3251 10
Size: 54 1425 343 1419 3294
Fragments arranged by size:
3294 1425 1419 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4493 4852 5011 518
Size: 648 3327 359 159 2042
Fragments arranged by size:
3327 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3163 3178 3291 3637 3740 3951 4628 5776
Size: 1557 15 113 346 103 211 677 1148
Cuts at: 5776 6021 1606
Size: 245 2120
Fragments arranged by size:
2120 1557 1148 677 346 245 211 113
103 15
NarI GG'CG_CC
Cuts at: 2235 2235
Size: 6535
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5044
Fragments arranged by size:
5044 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6535
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6535
NotI GC'GGCC_GC
Cuts at: 3982 3982
Size: 6535
NsiI A_TGCA'T
Cuts at: 2984 3757 2984
Size: 773 5762
NspI r_CATG'y
Cuts at: 1132 1229 3759 3893 5438 1132
Size: 97 2530 134 1545 2229
Fragments arranged by size:
2530 2229 1545 134 97
PasI CC'CwG_GG
Cuts at: 1348 3076 1348
Size: 1728 4807
PciI A'CATG_T
Cuts at: 5434 5434
Size: 6535
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3807 1108
Size: 2699 3836
PmeI GTTT'AAAC
Cuts at: 3839 3839
Size: 6535
PmlI CAC'GTG
Cuts at: 2593 3255 2593
Size: 662 5873
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4604 4636 6117 6149 4604
Size: 32 1481 32 4990
Fragments arranged by size:
4990 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2065 2295 2065
Size: 230 6305
PshAI GACnn'nnGTC
Cuts at: 1613 3095 3559 3824 1613
Size: 1482 464 265 4324
Fragments arranged by size:
4324 1482 464 265
PsiI TTA'TAA
Cuts at: 4105 4105
Size: 6535
PspOMI G'GGCC_C
Cuts at: 2653 2653
Size: 6535
PstI C_TGCA'G
Cuts at: 830 2136 2255 3183 3887 830
Size: 1306 119 928 704 3478
Fragments arranged by size:
3478 1306 928 704 119
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6535
PvuII CAG'CTG
Cuts at: 3163 3178 3637 3740 3163
Size: 15 459 103 5958
Fragments arranged by size:
5958 459 103 15
SacI G_AGCT'C
Cuts at: 3357 3357
Size: 6535
SalI G'TCGA_C
Cuts at: 1936 3877 1936
Size: 1941 4594
SanDI GG'GwC_CC
Cuts at: 2295 2295
Size: 6535
SbfI CC_TGCA'GG
Cuts at: 3887 3887
Size: 6535
ScaI AGT'ACT
Cuts at: 4783 6495 4783
Size: 1712 4823
SfcI C'TryA_G
Cuts at: 826 1045 2132 2251 3179 3883 5018 5699 5890
Size: 219 1087 119 928 704 1135 681 191
Cuts at: 5890 826
Size: 1471
Fragments arranged by size:
1471 1135 1087 928 704 681 219 191
119
SfoI GGC'GCC
Cuts at: 2236 2236
Size: 6535
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6535
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6535
SmaI CCC'GGG
Cuts at: 1872 3862 1872
Size: 1990 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2033 3411 4639 5540 5802 820
Size: 197 926 90 1378 1228 901 262 1553
Fragments arranged by size:
1553 1378 1228 926 901 262 197 90
SnaBI TAC'GTA
Cuts at: 493 4326 493
Size: 3833 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6535
SphI G_CATG'C
Cuts at: 1132 3759 3893 1132
Size: 2627 134 3774
Fragments arranged by size:
3774 2627 134
SspI AAT'ATT
Cuts at: 5 52 4459 5
Size: 47 4407 2081
Fragments arranged by size:
4407 2081 47
StuI AGG'CCT
Cuts at: 1517 2990 1517
Size: 1473 5062
StyI C'CwwG_G
Cuts at: 513 1065 2004 3309 3974 513
Size: 552 939 1305 665 3074
Fragments arranged by size:
3074 1305 939 665 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2842 4562 4579 4732 4917 5362 325
Size: 1255 1262 1720 17 153 185 445 1498
Fragments arranged by size:
1720 1498 1262 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2888 4781 6493
Size: 274 80 33 51 1443 911 1893 1712
Cuts at: 6493 96
Size: 138
Fragments arranged by size:
1893 1712 1443 911 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2716 2748 3270 3302 6495 6527 2716
Size: 32 522 32 3193 32 2724
Fragments arranged by size:
3193 2724 522 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6535
XbaI T'CTAG_A
Cuts at: 3871 3871
Size: 6535
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2665 2665
Size: 6535
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6535
XmaI C'CCGG_G
Cuts at: 1870 3860 1870
Size: 1990 4545
XmnI GAAnn'nnTTC
Cuts at: 4664 4664
Size: 6535
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4343 276
Size: 53 83 186 936 2809 2468
Fragments arranged by size:
2809 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BsiWI BstXI BstZ17I
EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI
PacI PfoI PspXI PsrI RsrII SacII SapI SexAI
SfiI SrfI SwaI