Restriction map for
pFN21AE6781
(Circular) (Six-base) MAPSORT of: pFN21AE6781.seq Check: 3653 from: 1 to: 6406
pFN21AE6781 6406 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3451 3451
Size: 6406
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2033 4216 278
Size: 53 83 186 936 497 2183 2468
Fragments arranged by size:
2468 2183 936 497 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3749 1937
Size: 1812 4594
Acc65I G'GTAC_C
Cuts at: 2327 3727 2327
Size: 1400 5006
AclI AA'CG_TT
Cuts at: 1563 4533 4906 1563
Size: 2970 373 3063
Fragments arranged by size:
3063 2970 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2039 2099 2271 3669 4469 5853 6121 1632
Size: 407 60 172 1398 800 1384 268 1917
Fragments arranged by size:
1917 1398 1384 800 407 268 172 60
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6209
AflIII A'CryG_T
Cuts at: 1203 5305 6282 1203
Size: 4102 977 1327
Fragments arranged by size:
4102 1327 977
AgeI A'CCGG_T
Cuts at: 5237 5237
Size: 6406
AhdI GACnn_n'nnGTC
Cuts at: 5135 5135
Size: 6406
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6406
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2758 3308 1184
Size: 1574 550 4282
Fragments arranged by size:
4282 1574 550
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2271 2303 2271
Size: 32 6374
AlwNI CAG_nnn'CTG
Cuts at: 2077 2170 2230 3133 3541 4149 5721 2077
Size: 93 60 903 408 608 1572 2762
Fragments arranged by size:
2762 1572 903 608 408 93 60
ApaLI G'TGCA_C
Cuts at: 4463 5619 4463
Size: 1156 5250
ApoI r'AATT_y
Cuts at: 1493 3715 3941 4093 6202 1493
Size: 2222 226 152 2109 1697
Fragments arranged by size:
2222 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4960 160
Size: 872 3928 1606
Fragments arranged by size:
3928 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6406
AvaI C'yCGr_G
Cuts at: 1870 1943 2382 3731 1870
Size: 73 439 1349 4545
Fragments arranged by size:
4545 1349 439 73
AvrII C'CTAG_G
Cuts at: 2710 2710
Size: 6406
BamHI G'GATC_C
Cuts at: 3736 4103 3736
Size: 367 6039
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2327 2334 2607 3027 3153
Size: 325 217 627 540 7 273 420 126
Cuts at: 3153 3727 5182 618
Size: 574 1455 1842
Fragments arranged by size:
1842 1455 627 574 540 420 325 273
217 126 7
BanII G_rGCy'C
Cuts at: 1391 2373 2397 2810 3725 1391
Size: 982 24 413 915 4072
Fragments arranged by size:
4072 982 915 413 24
BbeI G_GCGC'C
Cuts at: 2611 2611
Size: 6406
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2033 928
Size: 971 134 5301
Fragments arranged by size:
5301 971 134
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4597 4631 1903
Size: 34 50 34 2576 34 3678
Fragments arranged by size:
3678 2576 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4298 5508 4298
Size: 1210 5196
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6406
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2102 2468 3021
Size: 107 122 71 631 605 430 366 553
Cuts at: 3021 5017 136
Size: 1996 1525
Fragments arranged by size:
1996 1525 631 605 553 430 366 122
107 71
BglII A'GATC_T
Cuts at: 2820 6401 2820
Size: 3581 2825
BlpI GC'TnA_GC
Cuts at: 3823 3823
Size: 6406
Bme1580I G_kGCm'C
Cuts at: 1790 2404 2481 3187 4467 5623 1790
Size: 614 77 706 1280 1156 2573
Fragments arranged by size:
2573 1280 1156 706 614 77
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6406
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2291 4137 5095
Size: 416 53 83 399 24 874 1846 958
Cuts at: 5095 442
Size: 1753
Fragments arranged by size:
1846 1753 958 874 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2498 1056
Size: 1442 4964
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2237 2237
Size: 6406
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2207 2252 2312 2411 2432 2660 2794 3155
Size: 968 45 60 99 21 228 134 361
Cuts at: 3155 3317 3548 3566 5066 1239
Size: 162 231 18 1500 2579
Fragments arranged by size:
2579 1500 968 361 231 228 162 134
99 60 45 21 18
Bpu10I CC'TnA_GC
Cuts at: 2367 2367
Size: 6406
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3221 3296 3488 4531 5396 5694 3221
Size: 75 192 1043 865 298 3933
Fragments arranged by size:
3933 1043 865 298 192 75
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3206 5069 882
Size: 617 1707 1863 2219
Fragments arranged by size:
2219 1863 1707 617
BsaAI yAC'GTr
Cuts at: 493 1204 4197 493
Size: 711 2993 2702
Fragments arranged by size:
2993 2702 711
BsaBI GATnn'nnATC
Cuts at: 2819 6400 2819
Size: 3581 2825
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2014 2030 2608 3513
Size: 53 83 186 936 481 16 578 905
Cuts at: 3513 4213 4595 5230 275
Size: 700 382 635 1451
Fragments arranged by size:
1451 936 905 700 635 578 481 382
186 83 53 16
BsaWI w'CCGG_w
Cuts at: 4839 5237 5511 5658 6069 4839
Size: 398 274 147 411 5176
Fragments arranged by size:
5176 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3011 3041 1178
Size: 30 165 30 1608 30 4543
Fragments arranged by size:
4543 1608 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3605 1186
Size: 2419 3987
BseYI C'CCAG_C
Cuts at: 2439 2571 2583 2891 5609 6105 2439
Size: 132 12 308 2718 496 2740
Fragments arranged by size:
2740 2718 496 308 132 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2561 2669 3326 3429 1163
Size: 573 825 108 657 103 4140
Fragments arranged by size:
4140 825 657 573 108 103
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3856 4617 5645 1540
Size: 275 186 1855 761 1028 2301
Fragments arranged by size:
2301 1855 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2223 2397 3312 3372 3582 4467 4552 5623 2223
Size: 174 915 60 210 885 85 1071 3006
Fragments arranged by size:
3006 1071 915 885 210 174 85 60
BsmI GAATG_Cn'
Cuts at: 2659 2999 3917 4010 2659
Size: 340 918 93 5055
Fragments arranged by size:
5055 918 340 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2223 2373 2397 2404 2481 2810 3187
Size: 399 433 150 24 7 77 329 377
Cuts at: 3187 3312 3372 3582 3725 4467 4552 5623 1391
Size: 125 60 210 143 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 433 399 377 329 210
150 143 125 85 77 60 24 7
BspEI T'CCGG_A
Cuts at: 6069 6069
Size: 6406
BspHI T'CATG_A
Cuts at: 4295 6025 4295
Size: 1730 4676
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2922 3285 3451 3630 3761 844
Size: 2078 363 166 179 131 3489
Fragments arranged by size:
3489 2078 363 179 166 131
BsrBI CCG'CTC
Cuts at: 1394 1823 2425 2556 4293 1394
Size: 429 602 131 1737 3507
Fragments arranged by size:
3507 1737 602 429 131
BsrDI GCAATG_nn'
Cuts at: 65 1248 4901 5075 65
Size: 1183 3653 174 1396
Fragments arranged by size:
3653 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2886 5050 5237 1700
Size: 110 1076 2164 187 2869
Fragments arranged by size:
2869 2164 1076 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6406
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6406
BssSI C'ACGA_G
Cuts at: 4466 5478 4466
Size: 1012 5394
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3452 3541 794
Size: 2658 89 3659
Fragments arranged by size:
3659 2658 89
BstBI TT'CG_AA
Cuts at: 5210 5210
Size: 6406
BstEII G'GTnAC_C
Cuts at: 1184 3397 3629 1184
Size: 2213 232 3961
Fragments arranged by size:
3961 2213 232
BstYI r'GATC_y
Cuts at: 1972 2820 3358 3736 4103 4488 4505 5957 6043
Size: 848 538 378 367 385 17 1452 86
Cuts at: 6043 6055 6401 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 848 538 385 378 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2616 2616
Size: 6406
BtgI C'CryG_G
Cuts at: 513 1065 3243 513
Size: 552 2178 3676
Fragments arranged by size:
3676 2178 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2994 487
Size: 683 56 1768 3899
Fragments arranged by size:
3899 1768 683 56
BtsI GCAGTG_nn'
Cuts at: 2434 2735 3582 3922 4716 4736 2434
Size: 301 847 340 794 20 4104
Fragments arranged by size:
4104 847 794 340 301 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6406
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6371
DraI TTT'AAA
Cuts at: 3006 3710 3006
Size: 704 5702
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2242 5413 809
Size: 507 926 3171 1802
Fragments arranged by size:
3171 1802 926 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2460 3853 4742
Size: 54 1425 50 275 18 630 1393 889
Cuts at: 4742 6371 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1393 889 630 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3853 1537
Size: 275 2041 4090
Fragments arranged by size:
4090 2041 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2031 2247 2484 2545 4336 1353
Size: 678 216 237 61 1791 3423
Fragments arranged by size:
3423 1791 678 237 216 61
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2060 4993 5367 5513 387
Size: 1064 138 471 2933 374 146 1280
Fragments arranged by size:
2933 1280 1064 471 374 146 138
EcoICRI GAG'CTC
Cuts at: 2395 2395
Size: 6406
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2039 2099 2207 2252 2271 2312 2411
Size: 393 407 60 108 45 19 41 99
Cuts at: 2411 2432 2660 2794 3155 3317 3548 3566 3669
Size: 21 228 134 361 162 231 18 103
Cuts at: 3669 4469 5066 5853 6121 1239
Size: 800 597 787 268 1524
Fragments arranged by size:
1524 800 787 597 407 393 361 268
231 228 162 134 108 103 99 60
45 41 21 19 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2615 3601 2615
Size: 986 5420
EcoO109I rG'GnC_Cy
Cuts at: 2868 2868
Size: 6406
EcoRI G'AATT_C
Cuts at: 3715 4093 3715
Size: 378 6028
FspI TGC'GCA
Cuts at: 3281 4912 3281
Size: 1631 4775
HaeII r_GCGC'y
Cuts at: 1678 2122 2611 5553 1678
Size: 444 489 2942 2531
Fragments arranged by size:
2942 2531 489 444
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3095 3127 5047 5079 5121
Size: 32 135 32 1523 32 1920 32 42
Cuts at: 5121 5153 1373
Size: 32 2626
Fragments arranged by size:
2626 1920 1523 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3750 3996 669
Size: 988 281 1812 246 3079
Fragments arranged by size:
3079 1812 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6406
HpaI GTT'AAC
Cuts at: 3996 3996
Size: 6406
KasI G'GCGC_C
Cuts at: 2607 2607
Size: 6406
KpnI G_GTAC'C
Cuts at: 2331 3731 2331
Size: 1400 5006
MfeI C'AATT_G
Cuts at: 4005 4005
Size: 6406
MluI A'CGCG_T
Cuts at: 6282 6282
Size: 6406
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2556 5520 5704 1305
Size: 1251 2964 184 2007
Fragments arranged by size:
2964 2007 1251 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2462 10
Size: 54 1425 343 630 3954
Fragments arranged by size:
3954 1425 630 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4364 4723 4882 518
Size: 648 3198 359 159 2042
Fragments arranged by size:
3198 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2230 2575 3388 3637 3822 4499 5647 5892
Size: 624 345 813 249 185 677 1148 245
Cuts at: 5892 1606
Size: 2120
Fragments arranged by size:
2120 1148 813 677 624 345 249 245
185
NarI GG'CG_CC
Cuts at: 2608 2608
Size: 6406
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5854
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6406
NheI G'CTAG_C
Cuts at: 1052 2494 1052
Size: 1442 4964
NotI GC'GGCC_GC
Cuts at: 3853 3853
Size: 6406
NspI r_CATG'y
Cuts at: 1132 1229 2753 3764 5309 1132
Size: 97 1524 1011 1545 2229
Fragments arranged by size:
2229 1545 1524 1011 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6406
PciI A'CATG_T
Cuts at: 5305 5305
Size: 6406
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2170 2199 2230 3649 1108
Size: 1062 29 31 1419 3865
Fragments arranged by size:
3865 1419 1062 31 29
PfoI T'CCnGG_A
Cuts at: 2289 2409 2810 2289
Size: 120 401 5885
Fragments arranged by size:
5885 401 120
PmeI GTTT'AAAC
Cuts at: 3710 3710
Size: 6406
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3375 3407 4475 4507 5988 6020 3375
Size: 32 1068 32 1481 32 3761
Fragments arranged by size:
3761 1481 1068 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2868 2868
Size: 6406
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6406
PsiI TTA'TAA
Cuts at: 3976 3976
Size: 6406
PstI C_TGCA'G
Cuts at: 830 2488 3099 3129 3222 3480 3537 3577 3627
Size: 1658 611 30 93 258 57 40 50
Cuts at: 3627 3758 830
Size: 131 3478
Fragments arranged by size:
3478 1658 611 258 131 93 57 50
40 30
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6406
PvuII CAG'CTG
Cuts at: 2230 2575 3388 2230
Size: 345 813 5248
Fragments arranged by size:
5248 813 345
SacI G_AGCT'C
Cuts at: 2397 2397
Size: 6406
SalI G'TCGA_C
Cuts at: 1936 3748 1936
Size: 1812 4594
SapI GCTCTTCn'nnn_
Cuts at: 2031 2484 2031
Size: 453 5953
SbfI CC_TGCA'GG
Cuts at: 3627 3758 3627
Size: 131 6275
ScaI AGT'ACT
Cuts at: 3224 4654 6366 3224
Size: 1430 1712 3264
Fragments arranged by size:
3264 1712 1430
SfcI C'TryA_G
Cuts at: 826 1045 2484 3095 3125 3218 3476 3533 3573
Size: 219 1439 611 30 93 258 57 40
Cuts at: 3573 3623 3754 4889 5570 5761 826
Size: 50 131 1135 681 191 1471
Fragments arranged by size:
1471 1439 1135 681 611 258 219 191
131 93 57 50 40 30
SfoI GGC'GCC
Cuts at: 2609 2609
Size: 6406
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6406
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6406
SmaI CCC'GGG
Cuts at: 1872 3733 1872
Size: 1861 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3236 3311 3467 4510 5411 5673
Size: 197 926 1293 75 156 1043 901 262
Cuts at: 5673 820
Size: 1553
Fragments arranged by size:
1553 1293 1043 926 901 262 197 156
75
SnaBI TAC'GTA
Cuts at: 493 4197 493
Size: 3704 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6406
SphI G_CATG'C
Cuts at: 1132 3764 1132
Size: 2632 3774
SspI AAT'ATT
Cuts at: 5 52 4330 5
Size: 47 4278 2081
Fragments arranged by size:
4278 2081 47
StuI AGG'CCT
Cuts at: 1517 2768 1517
Size: 1251 5155
StyI C'CwwG_G
Cuts at: 513 1065 2710 3845 513
Size: 552 1645 1135 3074
Fragments arranged by size:
3074 1645 1135 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2114 3416 4433 4450 4603 4788 5233
Size: 1255 534 1302 1017 17 153 185 445
Cuts at: 5233 325
Size: 1498
Fragments arranged by size:
1498 1302 1255 1017 534 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3222 4652 6364
Size: 274 80 33 51 1443 1245 1430 1712
Cuts at: 6364 96
Size: 138
Fragments arranged by size:
1712 1443 1430 1245 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3011 3043 6366 6398 3011
Size: 32 3323 32 3019
Fragments arranged by size:
3323 3019 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3512 1316
Size: 2196 4210
XbaI T'CTAG_A
Cuts at: 3742 3742
Size: 6406
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2591 3502 2591
Size: 911 5495
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6406
XmaI C'CCGG_G
Cuts at: 1870 3731 1870
Size: 1861 4545
XmnI GAAnn'nnTTC
Cuts at: 2657 4535 2657
Size: 1878 4528
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2031 4214 276
Size: 53 83 186 936 497 2183 2468
Fragments arranged by size:
2468 2183 936 497 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI BaeI BbvCI BfrBI BsiWI BsmBI
BstXI BstZ17I DraIII EcoRV FalI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI
RsrII SacII SanDI SexAI SfiI SrfI SwaI