Restriction map for
pFN21AE6775
(Circular) (Six-base) MAPSORT of: pFN21AE6775.seq Check: 7301 from: 1 to: 6610
pFN21AE6775 6610 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2899 3893 2899
Size: 994 5616
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4420 278
Size: 53 83 186 936 2884 2468
Fragments arranged by size:
2884 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2069 3643 3953 1937
Size: 132 1574 310 4594
Fragments arranged by size:
4594 1574 310 132
Acc65I G'GTAC_C
Cuts at: 2844 3931 2844
Size: 1087 5523
AclI AA'CG_TT
Cuts at: 1563 4737 5110 1563
Size: 3174 373 3063
Fragments arranged by size:
3174 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2612 3059 3067 3198 4673 6057 6325 1632
Size: 980 447 8 131 1475 1384 268 1917
Fragments arranged by size:
1917 1475 1384 980 447 268 131 8
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6413
AflIII A'CryG_T
Cuts at: 1203 5509 6486 1203
Size: 4306 977 1327
Fragments arranged by size:
4306 1327 977
AgeI A'CCGG_T
Cuts at: 5441 5441
Size: 6610
AhdI GACnn_n'nnGTC
Cuts at: 5339 5339
Size: 6610
AleI CACnn'nnGTG
Cuts at: 1166 2057 3614 1166
Size: 891 1557 4162
Fragments arranged by size:
4162 1557 891
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6610
AlwNI CAG_nnn'CTG
Cuts at: 2519 2626 4353 5925 2519
Size: 107 1727 1572 3204
Fragments arranged by size:
3204 1727 1572 107
ApaLI G'TGCA_C
Cuts at: 4667 5823 4667
Size: 1156 5454
ApoI r'AATT_y
Cuts at: 1493 3919 4145 4297 6406 1493
Size: 2426 226 152 2109 1697
Fragments arranged by size:
2426 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5164 160
Size: 872 4132 1606
Fragments arranged by size:
4132 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6610
AvaI C'yCGr_G
Cuts at: 1870 1943 2047 2639 3183 3935 1870
Size: 73 104 592 544 752 4545
Fragments arranged by size:
4545 752 592 544 104 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2836 2869 2836
Size: 33 6577
BamHI G'GATC_C
Cuts at: 2743 3940 4307 2743
Size: 1197 367 5046
Fragments arranged by size:
5046 1197 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2844 3931 5386 618
Size: 325 217 627 1057 1087 1455 1842
Fragments arranged by size:
1842 1455 1087 1057 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2136 3066 3396 3446 3929 1391
Size: 745 930 330 50 483 4072
Fragments arranged by size:
4072 930 745 483 330 50
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2788 3150 928
Size: 971 889 362 4388
Fragments arranged by size:
4388 971 889 362
BbvCI CC'TCA_GC
Cuts at: 2026 2026
Size: 6610
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4801 4835 1903
Size: 34 2864 34 3678
Fragments arranged by size:
3678 2864 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2979 4502 5712 2979
Size: 1523 1210 3877
Fragments arranged by size:
3877 1523 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6610
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2937 3051 3556
Size: 107 122 71 631 605 1265 114 505
Cuts at: 3556 5221 136
Size: 1665 1525
Fragments arranged by size:
1665 1525 1265 631 605 505 122 114
107 71
BglII A'GATC_T
Cuts at: 2277 6605 2277
Size: 4328 2282
BlpI GC'TnA_GC
Cuts at: 4027 4027
Size: 6610
Bme1580I G_kGCm'C
Cuts at: 1790 2054 4671 5827 1790
Size: 264 2617 1156 2573
Fragments arranged by size:
2617 2573 1156 264
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6610
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3660 3689 4341
Size: 416 53 83 399 24 2243 29 652
Cuts at: 4341 5299 442
Size: 958 1753
Fragments arranged by size:
2243 1753 958 652 416 399 83 53
29 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6610
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2501 2501
Size: 6610
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2423 3430 3878 5270 1239
Size: 1184 1007 448 1392 2579
Fragments arranged by size:
2579 1392 1184 1007 448
Bpu10I CC'TnA_GC
Cuts at: 2026 2832 2991 2026
Size: 806 159 5645
Fragments arranged by size:
5645 806 159
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4735 5600 5898 4735
Size: 865 298 5447
Fragments arranged by size:
5447 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3893 5273 882
Size: 617 2394 1380 2219
Fragments arranged by size:
2394 2219 1380 617
BsaAI yAC'GTr
Cuts at: 493 1204 4401 493
Size: 711 3197 2702
Fragments arranged by size:
3197 2702 711
BsaBI GATnn'nnATC
Cuts at: 2465 3416 6604 2465
Size: 951 3188 2471
Fragments arranged by size:
3188 2471 951
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4417 4799 5434 275
Size: 53 83 186 936 2884 382 635 1451
Fragments arranged by size:
2884 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2212 5043 5441 5715 5862 6273 2212
Size: 2831 398 274 147 411 2549
Fragments arranged by size:
2831 2549 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6385
Fragments arranged by size:
6385 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6610
BseYI C'CCAG_C
Cuts at: 2154 2712 3319 3389 3817 5813 6309 2154
Size: 558 607 70 428 1996 496 2455
Fragments arranged by size:
2455 1996 607 558 496 428 70
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2328 2858 1163
Size: 573 592 530 4915
Fragments arranged by size:
4915 592 573 530
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2722 4060 4821 5849 1540
Size: 275 186 721 1338 761 1028 2301
Fragments arranged by size:
2301 1338 1028 761 721 275 186
BsiHKAI G_wGCw'C
Cuts at: 2024 2487 2880 3601 4671 4756 5827 2024
Size: 463 393 721 1070 85 1071 2807
Fragments arranged by size:
2807 1071 1070 721 463 393 85
BsmI GAATG_Cn'
Cuts at: 2979 3663 4121 4214 2979
Size: 684 458 93 5375
Fragments arranged by size:
5375 684 458 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2024 2054 2136 2487 2880 3066 3396
Size: 399 234 30 82 351 393 186 330
Cuts at: 3396 3446 3601 3929 4671 4756 5827 1391
Size: 50 155 328 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 393 351 330 328
234 186 155 85 82 50 30
BspEI T'CCGG_A
Cuts at: 6273 6273
Size: 6610
BspHI T'CATG_A
Cuts at: 2799 4499 6229 2799
Size: 1700 1730 3180
Fragments arranged by size:
3180 1730 1700
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2350 2899 3893 3965 844
Size: 1506 549 994 72 3489
Fragments arranged by size:
3489 1506 994 549 72
BsrBI CCG'CTC
Cuts at: 1394 1823 4497 1394
Size: 429 2674 3507
Fragments arranged by size:
3507 2674 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3069 5105 5279 65
Size: 1183 1821 2036 174 1396
Fragments arranged by size:
2036 1821 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2160 3666 5254 5441 1700
Size: 110 350 1506 1588 187 2869
Fragments arranged by size:
2869 1588 1506 350 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6610
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6610
BssSI C'ACGA_G
Cuts at: 4670 5682 4670
Size: 1012 5598
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6610
BstBI TT'CG_AA
Cuts at: 5414 5414
Size: 6610
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6610
BstXI CCAn_nnnn'nTGG
Cuts at: 3850 3850
Size: 6610
BstYI r'GATC_y
Cuts at: 1972 2277 2743 3031 3940 4307 4692 4709 6161
Size: 305 466 288 909 367 385 17 1452
Cuts at: 6161 6247 6259 6605 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 909 466 385 367 346 305
288 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2250 2703 3843 513
Size: 552 1185 453 1140 3280
Fragments arranged by size:
3280 1185 1140 552 453
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5871
Fragments arranged by size:
5871 683 56
BtsI GCAGTG_nn'
Cuts at: 2520 2898 3748 4126 4920 4940 2520
Size: 378 850 378 794 20 4190
Fragments arranged by size:
4190 850 794 378 378 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6610
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3601 3636 529
Size: 35 3037 35 3503
Fragments arranged by size:
3503 3037 35 35
DraI TTT'AAA
Cuts at: 3914 3914
Size: 6610
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3011 5617 809
Size: 507 1695 2606 1802
Fragments arranged by size:
2606 1802 1695 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2232 2253 2719
Size: 54 1425 50 275 18 402 21 466
Cuts at: 2719 3763 4057 4946 6575 8
Size: 1044 294 889 1629 43
Fragments arranged by size:
1629 1425 1044 889 466 402 294 275
54 50 43 21 18
EagI C'GGCC_G
Cuts at: 1537 1812 2719 4057 1537
Size: 275 907 1338 4090
Fragments arranged by size:
4090 1338 907 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3071 3086 3332 4540 1353
Size: 1718 15 246 1208 3423
Fragments arranged by size:
3423 1718 1208 246 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2204 5197 5571 5717 387
Size: 1064 138 615 2993 374 146 1280
Fragments arranged by size:
2993 1280 1064 615 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2423 2612 3059 3067 3198 3430 3878
Size: 393 791 189 447 8 131 232 448
Cuts at: 3878 4673 5270 6057 6325 1239
Size: 795 597 787 268 1524
Fragments arranged by size:
1524 795 791 787 597 448 447 393
268 232 189 131 8
EcoO109I rG'GnC_Cy
Cuts at: 2292 3043 3266 2292
Size: 751 223 5636
Fragments arranged by size:
5636 751 223
EcoRI G'AATT_C
Cuts at: 3919 4297 3919
Size: 378 6232
FspI TGC'GCA
Cuts at: 5116 5116
Size: 6610
HaeII r_GCGC'y
Cuts at: 1678 5757 1678
Size: 4079 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3625 3657 5251 5283 5325
Size: 32 135 32 2053 32 1594 32 42
Cuts at: 5325 5357 1373
Size: 32 2626
Fragments arranged by size:
2626 2053 1594 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3954 4200 669
Size: 988 281 2016 246 3079
Fragments arranged by size:
3079 2016 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6610
HpaI GTT'AAC
Cuts at: 4200 4200
Size: 6610
KpnI G_GTAC'C
Cuts at: 2848 3935 2848
Size: 1087 5523
MfeI C'AATT_G
Cuts at: 4209 4209
Size: 6610
MluI A'CGCG_T
Cuts at: 6486 6486
Size: 6610
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5724 5908 1305
Size: 4419 184 2007
Fragments arranged by size:
4419 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2234 2255 10
Size: 54 1425 343 402 21 4365
Fragments arranged by size:
4365 1425 402 343 54 21
MslI CAynn'nnrTG
Cuts at: 518 1166 2057 3524 3614 4568 4927 5086 518
Size: 648 891 1467 90 954 359 159 2042
Fragments arranged by size:
2042 1467 954 891 648 359 159 90
MspA1I CmG'CkG
Cuts at: 1606 2647 2842 3087 3316 3565 4026 4703 5851
Size: 1041 195 245 229 249 461 677 1148
Cuts at: 5851 6096 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 1041 677 461 249 245 245
229 195
NaeI GCC'GGC
Cuts at: 2162 2162
Size: 6610
NcoI C'CATG_G
Cuts at: 513 1065 3843 513
Size: 552 2778 3280
Fragments arranged by size:
3280 2778 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6610
NgoMIV G'CCGG_C
Cuts at: 2160 2160
Size: 6610
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6610
NotI GC'GGCC_GC
Cuts at: 4057 4057
Size: 6610
NspI r_CATG'y
Cuts at: 1132 1229 2068 3454 3968 5513 1132
Size: 97 839 1386 514 1545 2229
Fragments arranged by size:
2229 1545 1386 839 514 97
PasI CC'CwG_GG
Cuts at: 1348 2551 1348
Size: 1203 5407
PciI A'CATG_T
Cuts at: 5509 5509
Size: 6610
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2626 1108
Size: 1518 5092
PfoI T'CCnGG_A
Cuts at: 3894 3894
Size: 6610
PmeI GTTT'AAAC
Cuts at: 3914 3914
Size: 6610
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4679 4711 6192 6224 4679
Size: 32 1481 32 5065
Fragments arranged by size:
5065 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2292 3266 2292
Size: 974 5636
PshAI GACnn'nnGTC
Cuts at: 1613 2627 1613
Size: 1014 5596
PsiI TTA'TAA
Cuts at: 4180 4180
Size: 6610
PstI C_TGCA'G
Cuts at: 830 3279 3564 3755 3962 830
Size: 2449 285 191 207 3478
Fragments arranged by size:
3478 2449 285 207 191
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6610
PvuII CAG'CTG
Cuts at: 2647 2647
Size: 6610
RsrII CG'GwC_CG
Cuts at: 2191 2191
Size: 6610
SalI G'TCGA_C
Cuts at: 1936 3952 1936
Size: 2016 4594
SanDI GG'GwC_CC
Cuts at: 2292 2292
Size: 6610
SapI GCTCTTCn'nnn_
Cuts at: 3071 3071
Size: 6610
SbfI CC_TGCA'GG
Cuts at: 3962 3962
Size: 6610
ScaI AGT'ACT
Cuts at: 4858 6570 4858
Size: 1712 4898
SfcI C'TryA_G
Cuts at: 826 1045 3275 3560 3751 3958 5093 5774 5965
Size: 219 2230 285 191 207 1135 681 191
Cuts at: 5965 826
Size: 1471
Fragments arranged by size:
2230 1471 1135 681 285 219 207 191
191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3051 3051
Size: 6610
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6610
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6610
SmaI CCC'GGG
Cuts at: 1872 3185 3937 1872
Size: 1313 752 4545
Fragments arranged by size:
4545 1313 752
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4714 5615 5877 820
Size: 197 926 2771 901 262 1553
Fragments arranged by size:
2771 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4401 493
Size: 3908 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6610
SphI G_CATG'C
Cuts at: 1132 3454 3968 1132
Size: 2322 514 3774
Fragments arranged by size:
3774 2322 514
SrfI GCCC'GGGC
Cuts at: 3185 3185
Size: 6610
SspI AAT'ATT
Cuts at: 5 52 2300 4534 5
Size: 47 2248 2234 2081
Fragments arranged by size:
2248 2234 2081 47
StuI AGG'CCT
Cuts at: 1517 3054 3207 1517
Size: 1537 153 4920
Fragments arranged by size:
4920 1537 153
StyI C'CwwG_G
Cuts at: 513 1065 2673 2778 3158 3334 3843 4049 513
Size: 552 1608 105 380 176 509 206 3074
Fragments arranged by size:
3074 1608 552 509 380 206 176 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2252 2573 4637 4654 4807 4992 5437
Size: 1255 672 321 2064 17 153 185 445
Cuts at: 5437 325
Size: 1498
Fragments arranged by size:
2064 1498 1255 672 445 321 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3018 4856 6568
Size: 274 80 33 51 1443 1041 1838 1712
Cuts at: 6568 96
Size: 138
Fragments arranged by size:
1838 1712 1443 1041 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6570 6602 6570
Size: 32 6578
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6610
XbaI T'CTAG_A
Cuts at: 3946 3946
Size: 6610
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2147 3619 3779 2147
Size: 1472 160 4978
Fragments arranged by size:
4978 1472 160
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6610
XmaI C'CCGG_G
Cuts at: 1870 3183 3935 1870
Size: 1313 752 4545
Fragments arranged by size:
4545 1313 752
XmnI GAAnn'nnTTC
Cuts at: 4739 4739
Size: 6610
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4418 276
Size: 53 83 186 936 2884 2468
Fragments arranged by size:
2884 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII
SalI SanDI SapI SbfI ScaI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BfrBI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PmlI
PspOMI PspXI PsrI SacI SacII SexAI SfoI SwaI