Restriction map for
pFN21AE6774
(Circular) (Six-base) MAPSORT of: pFN21AE6774.seq Check: 1214 from: 1 to: 6481
pFN21AE6774 6481 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4291 278
Size: 53 83 186 936 2755 2468
Fragments arranged by size:
2755 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2151 3824 1937
Size: 214 1673 4594
Fragments arranged by size:
4594 1673 214
Acc65I G'GTAC_C
Cuts at: 3802 3802
Size: 6481
AclI AA'CG_TT
Cuts at: 1563 4608 4981 1563
Size: 3045 373 3063
Fragments arranged by size:
3063 3045 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2049 2882 4544 5928 6196 1632
Size: 417 833 1662 1384 268 1917
Fragments arranged by size:
1917 1662 1384 833 417 268
AfeI AGC'GCT
Cuts at: 2303 2303
Size: 6481
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6284
AflIII A'CryG_T
Cuts at: 1203 5380 6357 1203
Size: 4177 977 1327
Fragments arranged by size:
4177 1327 977
AgeI A'CCGG_T
Cuts at: 5312 5312
Size: 6481
AhdI GACnn_n'nnGTC
Cuts at: 5210 5210
Size: 6481
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6481
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6481
AlwNI CAG_nnn'CTG
Cuts at: 2386 3580 4224 5796 2386
Size: 1194 644 1572 3071
Fragments arranged by size:
3071 1572 1194 644
ApaI G_GGCC'C
Cuts at: 2417 2423 2417
Size: 6 6475
ApaLI G'TGCA_C
Cuts at: 4538 5694 4538
Size: 1156 5325
ApoI r'AATT_y
Cuts at: 1493 3093 3307 3790 4016 4168 6277 1493
Size: 1600 214 483 226 152 2109 1697
Fragments arranged by size:
2109 1697 1600 483 226 214 152
AseI AT'TA_AT
Cuts at: 160 1032 5035 160
Size: 872 4003 1606
Fragments arranged by size:
4003 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6481
AvaI C'yCGr_G
Cuts at: 1870 1943 2074 2319 2416 3806 1870
Size: 73 131 245 97 1390 4545
Fragments arranged by size:
4545 1390 245 131 97 73
BamHI G'GATC_C
Cuts at: 2254 3811 4178 2254
Size: 1557 367 4557
Fragments arranged by size:
4557 1557 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3802 5257 618
Size: 325 217 627 2015 1455 1842
Fragments arranged by size:
2015 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2083 2417 2423 2532 3396 3800 1391
Size: 692 334 6 109 864 404 4072
Fragments arranged by size:
4072 864 692 404 334 109 6
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5510
BbvCI CC'TCA_GC
Cuts at: 3160 3160
Size: 6481
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3176 3210 3235 3269 4672 4706 1903
Size: 34 1239 34 25 34 1403 34 3678
Fragments arranged by size:
3678 1403 1239 34 34 34 34 25
BciVI GTATCCnnnnn_n'
Cuts at: 3278 4373 5583 3278
Size: 1095 1210 4176
Fragments arranged by size:
4176 1210 1095
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6481
BfrBI ATG'CAT
Cuts at: 2557 2812 2557
Size: 255 6226
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2078 2412 3360
Size: 107 122 71 631 605 406 334 948
Cuts at: 3360 3595 5092 136
Size: 235 1497 1525
Fragments arranged by size:
1525 1497 948 631 605 406 334 235
122 107 71
BglII A'GATC_T
Cuts at: 6476 6476
Size: 6481
BlpI GC'TnA_GC
Cuts at: 3898 3898
Size: 6481
Bme1580I G_kGCm'C
Cuts at: 1790 2417 2423 2728 4542 5698 1790
Size: 627 6 305 1814 1156 2573
Fragments arranged by size:
2573 1814 1156 627 305 6
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6481
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2207 2789 3555
Size: 416 53 83 399 24 790 582 766
Cuts at: 3555 4212 5170 442
Size: 657 958 1753
Fragments arranged by size:
1753 958 790 766 657 582 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6481
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2599 2599
Size: 6481
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2026 2148 2546 3105 3186 3380 3705 5141
Size: 787 122 398 559 81 194 325 1436
Cuts at: 5141 1239
Size: 2579
Fragments arranged by size:
2579 1436 787 559 398 325 194 122
81
Bpu10I CC'TnA_GC
Cuts at: 2291 3160 2291
Size: 869 5612
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4606 5471 5769 4606
Size: 865 298 5318
Fragments arranged by size:
5318 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2131 2625 5144 882
Size: 617 632 494 2519 2219
Fragments arranged by size:
2519 2219 632 617 494
BsaAI yAC'GTr
Cuts at: 493 1204 4272 493
Size: 711 3068 2702
Fragments arranged by size:
3068 2702 711
BsaBI GATnn'nnATC
Cuts at: 2430 6475 2430
Size: 4045 2436
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4288 4670 5305 275
Size: 53 83 186 936 2755 382 635 1451
Fragments arranged by size:
2755 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2883 4914 5312 5586 5733 6144 2883
Size: 2031 398 274 147 411 3220
Fragments arranged by size:
3220 2031 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3103 3133 1178
Size: 30 165 30 1700 30 4526
Fragments arranged by size:
4526 1700 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3332 1186
Size: 2146 4335
BseYI C'CCAG_C
Cuts at: 2505 3389 5684 6180 2505
Size: 884 2295 496 2806
Fragments arranged by size:
2806 2295 884 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3439 1163
Size: 573 1703 4205
Fragments arranged by size:
4205 1703 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2930 3703 3931 4692 5720 1540
Size: 275 186 929 773 228 761 1028 2301
Fragments arranged by size:
2301 1028 929 773 761 275 228 186
BsiHKAI G_wGCw'C
Cuts at: 2204 2532 4542 4627 5698 2204
Size: 328 2010 85 1071 2987
Fragments arranged by size:
2987 2010 1071 328 85
BsmI GAATG_Cn'
Cuts at: 3234 3992 4085 3234
Size: 758 93 5630
Fragments arranged by size:
5630 758 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2083 2204 2417 2423 2532 2728 3396
Size: 399 293 121 213 6 109 196 668
Cuts at: 3396 3800 4542 4627 5698 1391
Size: 404 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 668 404 399 293 213
196 121 109 85 6
BspEI T'CCGG_A
Cuts at: 2883 6144 2883
Size: 3261 3220
BspHI T'CATG_A
Cuts at: 2269 2964 4370 6100 2269
Size: 695 1406 1730 2650
Fragments arranged by size:
2650 1730 1406 695
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2153 3588 3836 844
Size: 1309 1435 248 3489
Fragments arranged by size:
3489 1435 1309 248
BsrBI CCG'CTC
Cuts at: 1394 1823 4368 1394
Size: 429 2545 3507
Fragments arranged by size:
3507 2545 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3007 4976 5150 65
Size: 1183 1759 1969 174 1396
Fragments arranged by size:
1969 1759 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3698 5125 5312 1700
Size: 110 1888 1427 187 2869
Fragments arranged by size:
2869 1888 1427 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6481
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6481
BssSI C'ACGA_G
Cuts at: 3296 4541 5553 3296
Size: 1245 1012 4224
Fragments arranged by size:
4224 1245 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6481
BstBI TT'CG_AA
Cuts at: 2461 5285 2461
Size: 2824 3657
BstEII G'GTnAC_C
Cuts at: 1184 2308 3151 1184
Size: 1124 843 4514
Fragments arranged by size:
4514 1124 843
BstXI CCAn_nnnn'nTGG
Cuts at: 2305 3330 3373 2305
Size: 1025 43 5413
Fragments arranged by size:
5413 1025 43
BstYI r'GATC_y
Cuts at: 1972 2254 3811 4178 4563 4580 6032 6118 6130
Size: 282 1557 367 385 17 1452 86 12
Cuts at: 6130 6476 1972
Size: 346 1977
Fragments arranged by size:
1977 1557 1452 385 367 346 282 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2387 2387
Size: 6481
BtgI C'CryG_G
Cuts at: 513 1065 2407 3280 3407 3729 513
Size: 552 1342 873 127 322 3265
Fragments arranged by size:
3265 1342 873 552 322 127
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2929 487
Size: 683 56 1703 4039
Fragments arranged by size:
4039 1703 683 56
BtsI GCAGTG_nn'
Cuts at: 2684 3451 3997 4791 4811 2684
Size: 767 546 794 20 4354
Fragments arranged by size:
4354 794 767 546 20
ClaI AT'CG_AT
Cuts at: 1554 3520 1554
Size: 1966 4515
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6446
DraI TTT'AAA
Cuts at: 3785 3785
Size: 6481
DraIII CAC_nnn'GTG
Cuts at: 3298 3298
Size: 6481
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5488 809
Size: 507 4172 1802
Fragments arranged by size:
4172 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2815 2927 3022
Size: 54 1425 50 275 18 985 112 95
Cuts at: 3022 3234 3415 3634 3700 3928 4817 6446 8
Size: 212 181 219 66 228 889 1629 43
Fragments arranged by size:
1629 1425 985 889 275 228 219 212
181 112 95 66 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2927 3700 3928 1537
Size: 275 1115 773 228 4090
Fragments arranged by size:
4090 1115 773 275 228
EarI CTCTTCn'nnn_
Cuts at: 1353 2858 4411 1353
Size: 1505 1553 3423
Fragments arranged by size:
3423 1553 1505
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5068 5442 5588 387
Size: 1064 138 3479 374 146 1280
Fragments arranged by size:
3479 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2530 2530
Size: 6481
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2026 2049 2148 2546 2882 3105 3186
Size: 393 394 23 99 398 336 223 81
Cuts at: 3186 3380 3705 4544 5141 5928 6196 1239
Size: 194 325 839 597 787 268 1524
Fragments arranged by size:
1524 839 787 597 398 394 393 336
325 268 223 194 99 81 23
EcoO109I rG'GnC_Cy
Cuts at: 2413 3604 3663 2413
Size: 1191 59 5231
Fragments arranged by size:
5231 1191 59
EcoRI G'AATT_C
Cuts at: 3790 4168 3790
Size: 378 6103
FseI GG_CCGG'CC
Cuts at: 3702 3702
Size: 6481
FspI TGC'GCA
Cuts at: 4987 4987
Size: 6481
HaeII r_GCGC'y
Cuts at: 1678 2305 2751 3355 5628 1678
Size: 627 446 604 2273 2531
Fragments arranged by size:
2531 2273 627 604 446
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2659 2691 3059 3091 3433
Size: 32 135 32 1087 32 368 32 342
Cuts at: 3433 3465 3535 3567 5122 5154 5196 5228 1373
Size: 32 70 32 1555 32 42 32 2626
Fragments arranged by size:
2626 1555 1087 368 342 135 70 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3825 4071 669
Size: 988 281 1887 246 3079
Fragments arranged by size:
3079 1887 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6481
HpaI GTT'AAC
Cuts at: 4071 4071
Size: 6481
KpnI G_GTAC'C
Cuts at: 3806 3806
Size: 6481
MfeI C'AATT_G
Cuts at: 4080 4080
Size: 6481
MluI A'CGCG_T
Cuts at: 6357 6357
Size: 6481
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5595 5779 1305
Size: 4290 184 2007
Fragments arranged by size:
4290 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3236 3636 10
Size: 54 1425 343 1404 400 2855
Fragments arranged by size:
2855 1425 1404 400 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2514 2969 4439 4798 4957 518
Size: 648 843 505 455 1470 359 159 2042
Fragments arranged by size:
2042 1470 843 648 505 455 359 159
MspA1I CmG'CkG
Cuts at: 1606 2251 2409 2860 3164 3400 3897 4574 5722
Size: 645 158 451 304 236 497 677 1148
Cuts at: 5722 5967 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 645 497 451 304 245
236 158
NaeI GCC'GGC
Cuts at: 3700 3700
Size: 6481
NcoI C'CATG_G
Cuts at: 513 1065 3280 3729 513
Size: 552 2215 449 3265
Fragments arranged by size:
3265 2215 552 449
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6481
NgoMIV G'CCGG_C
Cuts at: 3698 3698
Size: 6481
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6481
NotI GC'GGCC_GC
Cuts at: 3928 3928
Size: 6481
NsiI A_TGCA'T
Cuts at: 2559 2814 2559
Size: 255 6226
NspI r_CATG'y
Cuts at: 1132 1229 3386 3839 5384 1132
Size: 97 2157 453 1545 2229
Fragments arranged by size:
2229 2157 1545 453 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6481
PciI A'CATG_T
Cuts at: 5380 5380
Size: 6481
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3413 1108
Size: 2305 4176
PfoI T'CCnGG_A
Cuts at: 2708 3121 3426 2708
Size: 413 305 5763
Fragments arranged by size:
5763 413 305
PmeI GTTT'AAAC
Cuts at: 3785 3785
Size: 6481
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2225 2257 4550 4582 6063 6095 2225
Size: 32 2293 32 1481 32 2611
Fragments arranged by size:
2611 2293 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3663 3663
Size: 6481
PshAI GACnn'nnGTC
Cuts at: 1613 3710 1613
Size: 2097 4384
PsiI TTA'TAA
Cuts at: 2637 4051 2637
Size: 1414 5067
PspOMI G'GGCC_C
Cuts at: 2413 2419 2413
Size: 6 6475
PstI C_TGCA'G
Cuts at: 830 2164 2250 2376 3202 3588 3833 830
Size: 1334 86 126 826 386 245 3478
Fragments arranged by size:
3478 1334 826 386 245 126 86
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6481
PvuII CAG'CTG
Cuts at: 2251 2860 3164 2251
Size: 609 304 5568
Fragments arranged by size:
5568 609 304
SacI G_AGCT'C
Cuts at: 2532 2532
Size: 6481
SacII CC_GC'GG
Cuts at: 2410 2410
Size: 6481
SalI G'TCGA_C
Cuts at: 1936 3823 1936
Size: 1887 4594
SanDI GG'GwC_CC
Cuts at: 3663 3663
Size: 6481
SapI GCTCTTCn'nnn_
Cuts at: 2858 2858
Size: 6481
SbfI CC_TGCA'GG
Cuts at: 3833 3833
Size: 6481
ScaI AGT'ACT
Cuts at: 4729 6441 4729
Size: 1712 4769
SfcI C'TryA_G
Cuts at: 826 1045 2148 2160 2246 2372 3198 3584 3829
Size: 219 1103 12 86 126 826 386 245
Cuts at: 3829 4964 5645 5836 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1103 826 681 386 245 219
191 126 86 12
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6481
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6481
SmaI CCC'GGG
Cuts at: 1872 2076 2321 2418 3808 1872
Size: 204 245 97 1390 4545
Fragments arranged by size:
4545 1390 245 204 97
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4585 5486 5748 820
Size: 197 926 2642 901 262 1553
Fragments arranged by size:
2642 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4272 493
Size: 3779 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6481
SphI G_CATG'C
Cuts at: 1132 3839 1132
Size: 2707 3774
SrfI GCCC'GGGC
Cuts at: 2418 2418
Size: 6481
SspI AAT'ATT
Cuts at: 5 52 2920 4405 5
Size: 47 2868 1485 2081
Fragments arranged by size:
2868 2081 1485 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6481
StyI C'CwwG_G
Cuts at: 513 1065 3280 3729 3920 513
Size: 552 2215 449 191 3074
Fragments arranged by size:
3074 2215 552 449 191
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2378 4508 4525 4678 4863 5308 325
Size: 1255 798 2130 17 153 185 445 1498
Fragments arranged by size:
2130 1498 1255 798 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2742 2760 2981
Size: 274 80 33 51 1443 765 18 221
Cuts at: 2981 4727 6439 96
Size: 1746 1712 138
Fragments arranged by size:
1746 1712 1443 765 274 221 138 80
51 33 18
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6441 6473 6441
Size: 32 6449
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6481
XbaI T'CTAG_A
Cuts at: 2623 3817 2623
Size: 1194 5287
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6481
XmaI C'CCGG_G
Cuts at: 1870 2074 2319 2416 3806 1870
Size: 204 245 97 1390 4545
Fragments arranged by size:
4545 1390 245 204 97
XmnI GAAnn'nnTTC
Cuts at: 4610 4610
Size: 6481
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4289 276
Size: 53 83 186 936 2755 2468
Fragments arranged by size:
2755 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII
SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BsiWI BsmBI
BstZ17I EcoNI EcoRV FalI FspAI KasI NarI NruI
PacI PmlI PspXI PsrI RsrII SexAI SfiI SfoI
SwaI XcmI