Restriction map for
pFN21AE6772
(Circular) (Six-base) MAPSORT of: pFN21AE6772.seq Check: 457 from: 1 to: 6544
pFN21AE6772 6544 bp
With 182 enzymes: SgfI *
July 24, 2018 19:59 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4354 278
Size: 53 83 186 936 2818 2468
Fragments arranged by size:
2818 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3887 1937
Size: 1950 4594
Acc65I G'GTAC_C
Cuts at: 3865 3865
Size: 6544
AclI AA'CG_TT
Cuts at: 1563 4671 5044 1563
Size: 3108 373 3063
Fragments arranged by size:
3108 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2340 2514 4607 5991 6259 1632
Size: 708 174 2093 1384 268 1917
Fragments arranged by size:
2093 1917 1384 708 268 174
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6347
AflIII A'CryG_T
Cuts at: 1203 5443 6420 1203
Size: 4240 977 1327
Fragments arranged by size:
4240 1327 977
AgeI A'CCGG_T
Cuts at: 5375 5375
Size: 6544
AhdI GACnn_n'nnGTC
Cuts at: 5273 5273
Size: 6544
AleI CACnn'nnGTG
Cuts at: 1166 2990 1166
Size: 1824 4720
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6544
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2355 2387 2355
Size: 32 6512
AlwNI CAG_nnn'CTG
Cuts at: 2050 2653 3054 3581 4287 5859 2050
Size: 603 401 527 706 1572 2735
Fragments arranged by size:
2735 1572 706 603 527 401
ApaI G_GGCC'C
Cuts at: 3467 3467
Size: 6544
ApaLI G'TGCA_C
Cuts at: 4601 5757 4601
Size: 1156 5388
ApoI r'AATT_y
Cuts at: 1493 2298 3853 4079 4231 6340 1493
Size: 805 1555 226 152 2109 1697
Fragments arranged by size:
2109 1697 1555 805 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5098 160
Size: 872 4066 1606
Fragments arranged by size:
4066 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6544
AvaI C'yCGr_G
Cuts at: 1870 1943 2086 2460 2973 3008 3869 1870
Size: 73 143 374 513 35 861 4545
Fragments arranged by size:
4545 861 513 374 143 73 35
BamHI G'GATC_C
Cuts at: 2464 3874 4241 2464
Size: 1410 367 4767
Fragments arranged by size:
4767 1410 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2053 2246 2386 3865 5320
Size: 325 217 627 266 193 140 1479 1455
Cuts at: 5320 618
Size: 1842
Fragments arranged by size:
1842 1479 1455 627 325 266 217 193
140
BanII G_rGCy'C
Cuts at: 1391 2461 3210 3467 3863 1391
Size: 1070 749 257 396 4072
Fragments arranged by size:
4072 1070 749 396 257
BbeI G_GCGC'C
Cuts at: 2390 2390
Size: 6544
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3846 928
Size: 971 1947 3626
Fragments arranged by size:
3626 1947 971
BbvCI CC'TCA_GC
Cuts at: 3047 3047
Size: 6544
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4735 4769 1903
Size: 34 2798 34 3678
Fragments arranged by size:
3678 2798 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3596 4436 5646 3596
Size: 840 1210 4494
Fragments arranged by size:
4494 1210 840
BclI T'GATC_A
Cuts at: 1548 3370 1548
Size: 1822 4722
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3433 5155 136
Size: 107 122 71 631 605 1761 1722 1525
Fragments arranged by size:
1761 1722 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3802 6539 3802
Size: 2737 3807
BlpI GC'TnA_GC
Cuts at: 3423 3961 3423
Size: 538 6006
Bme1580I G_kGCm'C
Cuts at: 1790 2058 2987 3467 4605 5761 1790
Size: 268 929 480 1138 1156 2573
Fragments arranged by size:
2573 1156 1138 929 480 268
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6544
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2700 4275 5233
Size: 416 53 83 399 24 1283 1575 958
Cuts at: 5233 442
Size: 1753
Fragments arranged by size:
1753 1575 1283 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2172 1056
Size: 1116 5428
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2132 2844 3224 3420 3435 3472 5204 1239
Size: 893 712 380 196 15 37 1732 2579
Fragments arranged by size:
2579 1732 893 712 380 196 37 15
Bpu10I CC'TnA_GC
Cuts at: 2502 2646 3047 2502
Size: 144 401 5999
Fragments arranged by size:
5999 401 144
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2361 4669 5534 5832 2361
Size: 2308 865 298 3073
Fragments arranged by size:
3073 2308 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5207 882
Size: 617 3708 2219
Fragments arranged by size:
3708 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4335 493
Size: 711 3131 2702
Fragments arranged by size:
3131 2702 711
BsaBI GATnn'nnATC
Cuts at: 6538 6538
Size: 6544
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2387 4351 4733 5368
Size: 53 83 186 936 854 1964 382 635
Cuts at: 5368 275
Size: 1451
Fragments arranged by size:
1964 1451 936 854 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2010 4977 5375 5649 5796 6207 2010
Size: 2967 398 274 147 411 2347
Fragments arranged by size:
2967 2347 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2355 2385 2817 2847 3619
Size: 30 165 30 952 30 432 30 772
Cuts at: 3619 3649 1178
Size: 30 4073
Fragments arranged by size:
4073 952 772 432 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2514 1186
Size: 1328 5216
BseYI C'CCAG_C
Cuts at: 3638 5747 6243 3638
Size: 2109 496 3939
Fragments arranged by size:
3939 2109 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3525 1163
Size: 573 1789 4182
Fragments arranged by size:
4182 1789 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3994 4755 5783 1540
Size: 275 186 1993 761 1028 2301
Fragments arranged by size:
2301 1993 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2959 3366 4605 4690 5761 2959
Size: 407 1239 85 1071 3742
Fragments arranged by size:
3742 1239 1071 407 85
BsmI GAATG_Cn'
Cuts at: 3762 4055 4148 3762
Size: 293 93 6158
Fragments arranged by size:
6158 293 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2058 2461 2959 2987 3210 3366 3467
Size: 399 268 403 498 28 223 156 101
Cuts at: 3467 3863 4605 4690 5761 1391
Size: 396 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 498 403 399 396 268
223 156 101 85 28
BspEI T'CCGG_A
Cuts at: 6207 6207
Size: 6544
BspHI T'CATG_A
Cuts at: 3835 4433 6163 3835
Size: 598 1730 4216
Fragments arranged by size:
4216 1730 598
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2661 3899 844
Size: 1817 1238 3489
Fragments arranged by size:
3489 1817 1238
BsrBI CCG'CTC
Cuts at: 1394 1823 4431 1394
Size: 429 2608 3507
Fragments arranged by size:
3507 2608 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5039 5213 65
Size: 1183 3791 174 1396
Fragments arranged by size:
3791 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2100 5188 5375 1700
Size: 110 290 3088 187 2869
Fragments arranged by size:
3088 2869 290 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6544
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6544
BssSI C'ACGA_G
Cuts at: 4604 5616 4604
Size: 1012 5532
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3317 794
Size: 2523 4021
BstBI TT'CG_AA
Cuts at: 5348 5348
Size: 6544
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6544
BstYI r'GATC_y
Cuts at: 1972 2447 2464 2497 3030 3802 3874 4241 4626
Size: 475 17 33 533 772 72 367 385
Cuts at: 4626 4643 6095 6181 6193 6539 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 772 533 475 385 367 346
86 72 33 17 17 12
Bsu36I CC'TnA_GG
Cuts at: 3707 3707
Size: 6544
BtgI C'CryG_G
Cuts at: 513 1065 3060 513
Size: 552 1995 3997
Fragments arranged by size:
3997 1995 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2140 487
Size: 683 56 914 4891
Fragments arranged by size:
4891 914 683 56
BtsI GCAGTG_nn'
Cuts at: 2573 3136 4060 4854 4874 2573
Size: 563 924 794 20 4243
Fragments arranged by size:
4243 924 794 563 20
ClaI AT'CG_AT
Cuts at: 1554 2196 1554
Size: 642 5902
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3214 3249 529
Size: 35 2650 35 3824
Fragments arranged by size:
3824 2650 35 35
DraI TTT'AAA
Cuts at: 3848 3848
Size: 6544
DraIII CAC_nnn'GTG
Cuts at: 3187 3187
Size: 6544
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2561 3719 5551 809
Size: 507 1245 1158 1832 1802
Fragments arranged by size:
1832 1802 1245 1158 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3222 3991 4880
Size: 54 1425 50 275 18 1392 769 889
Cuts at: 4880 6509 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1392 889 769 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3991 1537
Size: 275 2179 4090
Fragments arranged by size:
4090 2179 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2831 3008 4474 1353
Size: 1478 177 1466 3423
Fragments arranged by size:
3423 1478 1466 177
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5131 5505 5651 387
Size: 1064 138 3542 374 146 1280
Fragments arranged by size:
3542 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2132 2340 2514 2844 3224 3420 3435
Size: 393 500 208 174 330 380 196 15
Cuts at: 3435 3472 4607 5204 5991 6259 1239
Size: 37 1135 597 787 268 1524
Fragments arranged by size:
1524 1135 787 597 500 393 380 330
268 208 196 174 37 15
EcoO109I rG'GnC_Cy
Cuts at: 2650 3464 2650
Size: 814 5730
EcoRI G'AATT_C
Cuts at: 3853 4231 3853
Size: 378 6166
FspI TGC'GCA
Cuts at: 5050 5050
Size: 6544
HaeII r_GCGC'y
Cuts at: 1678 2390 5691 1678
Size: 712 3301 2531
Fragments arranged by size:
3301 2531 712
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3593 3625 5185 5217 5259
Size: 32 135 32 2021 32 1560 32 42
Cuts at: 5259 5291 1373
Size: 32 2626
Fragments arranged by size:
2626 2021 1560 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3888 4134 669
Size: 988 281 1950 246 3079
Fragments arranged by size:
3079 1950 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2878 748
Size: 2130 4414
HpaI GTT'AAC
Cuts at: 4134 4134
Size: 6544
KasI G'GCGC_C
Cuts at: 2386 2386
Size: 6544
KpnI G_GTAC'C
Cuts at: 3869 3869
Size: 6544
MfeI C'AATT_G
Cuts at: 4143 4143
Size: 6544
MluI A'CGCG_T
Cuts at: 6420 6420
Size: 6544
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3100 5658 5842 1305
Size: 1795 2558 184 2007
Fragments arranged by size:
2558 2007 1795 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3224 10
Size: 54 1425 343 1392 3330
Fragments arranged by size:
3330 1425 1392 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2990 3305 4502 4861 5020 518
Size: 648 1824 315 1197 359 159 2042
Fragments arranged by size:
2042 1824 1197 648 359 315 159
MspA1I CmG'CkG
Cuts at: 1606 2429 2710 3194 3960 4637 5785 6030 1606
Size: 823 281 484 766 677 1148 245 2120
Fragments arranged by size:
2120 1148 823 766 677 484 281 245
NarI GG'CG_CC
Cuts at: 2387 2387
Size: 6544
NcoI C'CATG_G
Cuts at: 513 1065 3060 513
Size: 552 1995 3997
Fragments arranged by size:
3997 1995 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6544
NheI G'CTAG_C
Cuts at: 1052 2168 1052
Size: 1116 5428
NotI GC'GGCC_GC
Cuts at: 3991 3991
Size: 6544
NspI r_CATG'y
Cuts at: 1132 1229 2135 2518 3327 3902 5447 1132
Size: 97 906 383 809 575 1545 2229
Fragments arranged by size:
2229 1545 906 809 575 383 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6544
PciI A'CATG_T
Cuts at: 5443 5443
Size: 6544
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3187 1108
Size: 2079 4465
PmeI GTTT'AAAC
Cuts at: 3848 3848
Size: 6544
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2355 2387 4613 4645 6126 6158 2355
Size: 32 2226 32 1481 32 2741
Fragments arranged by size:
2741 2226 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2650 2650
Size: 6544
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6544
PsiI TTA'TAA
Cuts at: 4114 4114
Size: 6544
PspOMI G'GGCC_C
Cuts at: 3463 3463
Size: 6544
PspXI vC'TCGA_Gb
Cuts at: 2973 2973
Size: 6544
PstI C_TGCA'G
Cuts at: 830 2443 3896 830
Size: 1613 1453 3478
Fragments arranged by size:
3478 1613 1453
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6544
PvuII CAG'CTG
Cuts at: 2429 2710 3194 2429
Size: 281 484 5779
Fragments arranged by size:
5779 484 281
RsrII CG'GwC_CG
Cuts at: 2067 2067
Size: 6544
SalI G'TCGA_C
Cuts at: 1936 3886 1936
Size: 1950 4594
SbfI CC_TGCA'GG
Cuts at: 3896 3896
Size: 6544
ScaI AGT'ACT
Cuts at: 4792 6504 4792
Size: 1712 4832
SfcI C'TryA_G
Cuts at: 826 1045 2439 3892 5027 5708 5899 826
Size: 219 1394 1453 1135 681 191 1471
Fragments arranged by size:
1471 1453 1394 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2388 2388
Size: 6544
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6544
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6544
SmaI CCC'GGG
Cuts at: 1872 3871 1872
Size: 1999 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2376 2973 4648 5549 5811 820
Size: 197 926 433 597 1675 901 262 1553
Fragments arranged by size:
1675 1553 926 901 597 433 262 197
SnaBI TAC'GTA
Cuts at: 493 4335 493
Size: 3842 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6544
SphI G_CATG'C
Cuts at: 1132 3327 3902 1132
Size: 2195 575 3774
Fragments arranged by size:
3774 2195 575
SspI AAT'ATT
Cuts at: 5 52 4468 5
Size: 47 4416 2081
Fragments arranged by size:
4416 2081 47
StuI AGG'CCT
Cuts at: 1517 2022 3617 1517
Size: 505 1595 4444
Fragments arranged by size:
4444 1595 505
StyI C'CwwG_G
Cuts at: 513 1065 2058 2935 2979 3060 3983 513
Size: 552 993 877 44 81 923 3074
Fragments arranged by size:
3074 993 923 877 552 81 44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3295 4571 4588 4741 4926 5371 325
Size: 1255 1715 1276 17 153 185 445 1498
Fragments arranged by size:
1715 1498 1276 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2704 4790 6502
Size: 274 80 33 51 1443 727 2086 1712
Cuts at: 6502 96
Size: 138
Fragments arranged by size:
2086 1712 1443 727 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2817 2849 6504 6536 2817
Size: 32 3655 32 2825
Fragments arranged by size:
3655 2825 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3607 1316
Size: 2291 4253
XbaI T'CTAG_A
Cuts at: 2140 3880 2140
Size: 1740 4804
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3217 3217
Size: 6544
XhoI C'TCGA_G
Cuts at: 1943 2973 1943
Size: 1030 5514
XmaI C'CCGG_G
Cuts at: 1870 3869 1870
Size: 1999 4545
XmnI GAAnn'nnTTC
Cuts at: 2994 4673 2994
Size: 1679 4865
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4352 276
Size: 53 83 186 936 2818 2468
Fragments arranged by size:
2818 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII RsrII SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstXI BstZ17I EcoICRI EcoNI EcoRV FalI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI
PsrI SacI SacII SanDI SapI SexAI SfiI SrfI
SwaI