Restriction map for
pFN21AE6763
(Circular) (Six-base) MAPSORT of: pFN21AE6763.seq Check: 8004 from: 1 to: 7225
pFN21AE6763 7225 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2952 3813 4161 5035
Size: 53 83 186 936 1416 861 348 874
Cuts at: 5035 278
Size: 2468
Fragments arranged by size:
2468 1416 936 874 861 348 186 83
53
AccI GT'mk_AC
Cuts at: 1937 2742 2919 4568 1937
Size: 805 177 1649 4594
Fragments arranged by size:
4594 1649 805 177
Acc65I G'GTAC_C
Cuts at: 4546 4546
Size: 7225
AclI AA'CG_TT
Cuts at: 1563 5352 5725 1563
Size: 3789 373 3063
Fragments arranged by size:
3789 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2417 3685 4289 4290 5288 6672 6940 1632
Size: 785 1268 604 1 998 1384 268 1917
Fragments arranged by size:
1917 1384 1268 998 785 604 268 1
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7028
AflIII A'CryG_T
Cuts at: 1203 2592 2934 3002 3858 6124 7101 1203
Size: 1389 342 68 856 2266 977 1327
Fragments arranged by size:
2266 1389 1327 977 856 342 68
AgeI A'CCGG_T
Cuts at: 6056 6056
Size: 7225
AhdI GACnn_n'nnGTC
Cuts at: 5954 5954
Size: 7225
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7225
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7225
AlwNI CAG_nnn'CTG
Cuts at: 2492 2854 4968 6540 2492
Size: 362 2114 1572 3177
Fragments arranged by size:
3177 2114 1572 362
ApaI G_GGCC'C
Cuts at: 4243 4243
Size: 7225
ApaLI G'TGCA_C
Cuts at: 5282 6438 5282
Size: 1156 6069
ApoI r'AATT_y
Cuts at: 1493 2153 2652 3116 4534 4760 4912 7021 1493
Size: 660 499 464 1418 226 152 2109 1697
Fragments arranged by size:
2109 1697 1418 660 499 464 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5779 160
Size: 872 4747 1606
Fragments arranged by size:
4747 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7225
AvaI C'yCGr_G
Cuts at: 1870 1943 2246 4550 1870
Size: 73 303 2304 4545
Fragments arranged by size:
4545 2304 303 73
BamHI G'GATC_C
Cuts at: 2632 4029 4555 4922 2632
Size: 1397 526 367 4935
Fragments arranged by size:
4935 1397 526 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4546 6001 618
Size: 325 217 627 2759 1455 1842
Fragments arranged by size:
2759 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2091 2100 4123 4243 4544 1391
Size: 700 9 2023 120 301 4072
Fragments arranged by size:
4072 2023 700 301 120 9
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2217 3317 928
Size: 971 318 1100 4836
Fragments arranged by size:
4836 1100 971 318
BbvCI CC'TCA_GC
Cuts at: 3677 4244 3677
Size: 567 6658
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5416 5450 1903
Size: 34 3479 34 3678
Fragments arranged by size:
3678 3479 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3761 5117 6327 3761
Size: 1356 1210 4659
Fragments arranged by size:
4659 1356 1210
BclI T'GATC_A
Cuts at: 1548 2532 3783 4386 1548
Size: 984 1251 603 4387
Fragments arranged by size:
4387 1251 984 603
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4089 5836 136
Size: 107 122 71 631 605 2417 1747 1525
Fragments arranged by size:
2417 1747 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2715 2976 7220 2715
Size: 261 4244 2720
Fragments arranged by size:
4244 2720 261
BlpI GC'TnA_GC
Cuts at: 2085 4642 2085
Size: 2557 4668
Bme1580I G_kGCm'C
Cuts at: 1790 3019 4243 5286 6442 1790
Size: 1229 1224 1043 1156 2573
Fragments arranged by size:
2573 1229 1224 1156 1043
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7225
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4441 4956 5914
Size: 416 53 83 399 24 3024 515 958
Cuts at: 5914 442
Size: 1753
Fragments arranged by size:
3024 1753 958 515 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7225
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2711 4417 2711
Size: 1706 5519
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2039 2207 2312 2495 2648 3041 4054 5885
Size: 800 168 105 183 153 393 1013 1831
Cuts at: 5885 1239
Size: 2579
Fragments arranged by size:
2579 1831 1013 800 393 183 168 153
105
Bpu10I CC'TnA_GC
Cuts at: 2696 3677 4244 2696
Size: 981 567 5677
Fragments arranged by size:
5677 981 567
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5350 6215 6513 5350
Size: 865 298 6062
Fragments arranged by size:
6062 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5888 882
Size: 617 4389 2219
Fragments arranged by size:
4389 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3005 3817 5016 493
Size: 711 1801 812 1199 2702
Fragments arranged by size:
2702 1801 1199 812 711
BsaBI GATnn'nnATC
Cuts at: 3749 7219 3749
Size: 3470 3755
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2619 2949 3810 4158
Size: 53 83 186 936 1086 330 861 348
Cuts at: 4158 5032 5414 6049 275
Size: 874 382 635 1451
Fragments arranged by size:
1451 1086 936 874 861 635 382 348
330 186 83 53
BsaWI w'CCGG_w
Cuts at: 2504 5658 6056 6330 6477 6888 2504
Size: 3154 398 274 147 411 2841
Fragments arranged by size:
3154 2841 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2037 2067 2867 2897 3076
Size: 30 165 30 634 30 800 30 179
Cuts at: 3076 3106 3398 3428 4205 4235 4314 4344 1178
Size: 30 292 30 777 30 79 30 4059
Fragments arranged by size:
4059 800 777 634 292 179 165 79
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2042 3037 4535 1186
Size: 856 995 1498 3876
Fragments arranged by size:
3876 1498 995 856
BseYI C'CCAG_C
Cuts at: 2955 3181 3523 3833 4011 4317 6428 6924 2955
Size: 226 342 310 178 306 2111 496 3256
Fragments arranged by size:
3256 2111 496 342 310 306 226 178
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2921 3252 4397 4412 1163
Size: 573 1185 331 1145 15 3976
Fragments arranged by size:
3976 1185 1145 573 331 15
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4675 5436 6464 1540
Size: 275 186 2674 761 1028 2301
Fragments arranged by size:
2674 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2091 2100 4278 5286 5371 6442 2091
Size: 9 2178 1008 85 1071 2874
Fragments arranged by size:
2874 2178 1071 1008 85 9
BsmI GAATG_Cn'
Cuts at: 4736 4829 4736
Size: 93 7132
BsmBI CGTCTCn'nnnn_
Cuts at: 2414 3286 4149 2414
Size: 872 863 5490
Fragments arranged by size:
5490 872 863
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2091 2100 3019 4123 4243 4278 4544
Size: 399 301 9 919 1104 120 35 266
Cuts at: 4544 5286 5371 6442 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1104 1071 919 742 399 301 266
120 85 35 9
BspEI T'CCGG_A
Cuts at: 6888 6888
Size: 7225
BspHI T'CATG_A
Cuts at: 3063 3780 5114 6844 3063
Size: 717 1334 1730 3444
Fragments arranged by size:
3444 1730 1334 717
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4236 4580 844
Size: 3392 344 3489
Fragments arranged by size:
3489 3392 344
BsrBI CCG'CTC
Cuts at: 1394 1823 2269 2563 5112 1394
Size: 429 446 294 2549 3507
Fragments arranged by size:
3507 2549 446 429 294
BsrDI GCAATG_nn'
Cuts at: 65 1248 5720 5894 65
Size: 1183 4472 174 1396
Fragments arranged by size:
4472 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2525 5869 6056 1700
Size: 110 715 3344 187 2869
Fragments arranged by size:
3344 2869 715 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7225
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7225
BssSI C'ACGA_G
Cuts at: 5285 6297 5285
Size: 1012 6213
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2492 4420 794
Size: 1698 1928 3599
Fragments arranged by size:
3599 1928 1698
BstBI TT'CG_AA
Cuts at: 2020 6029 2020
Size: 4009 3216
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7225
BstYI r'GATC_y
Cuts at: 1972 2632 2715 2976 4029 4555 4922 5307 5324
Size: 660 83 261 1053 526 367 385 17
Cuts at: 5324 6776 6862 6874 7220 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1053 660 526 385 367 346
261 86 83 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2273 3051 3343 3959 4114 4132
Size: 552 939 269 778 292 616 155 18
Cuts at: 4132 513
Size: 3606
Fragments arranged by size:
3606 939 778 616 552 292 269 155
18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2953 487
Size: 683 56 1727 4759
Fragments arranged by size:
4759 1727 683 56
BtsI GCAGTG_nn'
Cuts at: 2493 2966 4741 5535 5555 2493
Size: 473 1775 794 20 4163
Fragments arranged by size:
4163 1775 794 473 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7225
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7190
DraI TTT'AAA
Cuts at: 2124 4495 4529 2124
Size: 2371 34 4820
Fragments arranged by size:
4820 2371 34
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4163 6232 809
Size: 507 2847 2069 1802
Fragments arranged by size:
2847 2069 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4090 4672 5561
Size: 54 1425 50 275 18 2260 582 889
Cuts at: 5561 7190 8
Size: 1629 43
Fragments arranged by size:
2260 1629 1425 889 582 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4672 1537
Size: 275 2860 4090
Fragments arranged by size:
4090 2860 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3445 3889 4134 5155 1353
Size: 2092 444 245 1021 3423
Fragments arranged by size:
3423 2092 1021 444 245
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5812 6186 6332 387
Size: 1064 138 4223 374 146 1280
Fragments arranged by size:
4223 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2089 2098 2089
Size: 9 7216
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2039 2207 2312 2417 2495 2648 3041
Size: 393 407 168 105 105 78 153 393
Cuts at: 3041 3685 4054 4289 4290 5288 5885 6672 6940
Size: 644 369 235 1 998 597 787 268
Cuts at: 6940 1239
Size: 1524
Fragments arranged by size:
1524 998 787 644 597 407 393 393
369 268 235 168 153 105 105 78
1
EcoNI CCTnn'n_nnAGG
Cuts at: 2858 2858
Size: 7225
EcoO109I rG'GnC_Cy
Cuts at: 4240 4396 4240
Size: 156 7069
EcoRI G'AATT_C
Cuts at: 4534 4912 4534
Size: 378 6847
FspI TGC'GCA
Cuts at: 5731 5731
Size: 7225
HaeII r_GCGC'y
Cuts at: 1678 2854 3352 6372 1678
Size: 1176 498 3020 2531
Fragments arranged by size:
3020 2531 1176 498
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2724 2756 3285 3317 3398
Size: 32 135 32 1152 32 529 32 81
Cuts at: 3398 3430 4314 4346 5866 5898 5940 5972 1373
Size: 32 884 32 1520 32 42 32 2626
Fragments arranged by size:
2626 1520 1152 884 529 135 81 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4569 4815 669
Size: 988 281 2631 246 3079
Fragments arranged by size:
3079 2631 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2362 748
Size: 1614 5611
HpaI GTT'AAC
Cuts at: 4815 4815
Size: 7225
KpnI G_GTAC'C
Cuts at: 4550 4550
Size: 7225
MfeI C'AATT_G
Cuts at: 4824 4824
Size: 7225
MluI A'CGCG_T
Cuts at: 7101 7101
Size: 7225
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2763 3478 3912 6339 6523 1305
Size: 1458 715 434 2427 184 2007
Fragments arranged by size:
2427 2007 1458 715 434 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4092 10
Size: 54 1425 343 2260 3143
Fragments arranged by size:
3143 2260 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2897 3857 5183 5542 5701 518
Size: 648 1731 960 1326 359 159 2042
Fragments arranged by size:
2042 1731 1326 960 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2870 3125 3517 4015 4299 4641 5318 6466
Size: 1264 255 392 498 284 342 677 1148
Cuts at: 6466 6711 1606
Size: 245 2120
Fragments arranged by size:
2120 1264 1148 677 498 392 342 284
255 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3051 4132 513
Size: 552 939 1047 1081 3606
Fragments arranged by size:
3606 1081 1047 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7225
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7225
NotI GC'GGCC_GC
Cuts at: 4672 4672
Size: 7225
NspI r_CATG'y
Cuts at: 1132 1229 2938 3862 4583 6128 1132
Size: 97 1709 924 721 1545 2229
Fragments arranged by size:
2229 1709 1545 924 721 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7225
PciI A'CATG_T
Cuts at: 2934 3858 6124 2934
Size: 924 2266 4035
Fragments arranged by size:
4035 2266 924
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7225
PfoI T'CCnGG_A
Cuts at: 2472 4025 4503 2472
Size: 1553 478 5194
Fragments arranged by size:
5194 1553 478
PmeI GTTT'AAAC
Cuts at: 4529 4529
Size: 7225
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2966 2998 5294 5326 6807 6839 2966
Size: 32 2296 32 1481 32 3352
Fragments arranged by size:
3352 2296 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3240 1613
Size: 1627 5598
PsiI TTA'TAA
Cuts at: 4795 4795
Size: 7225
PspOMI G'GGCC_C
Cuts at: 4239 4239
Size: 7225
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2588 2620 2588
Size: 32 7193
PstI C_TGCA'G
Cuts at: 830 2862 3516 4020 4108 4577 830
Size: 2032 654 504 88 469 3478
Fragments arranged by size:
3478 2032 654 504 469 88
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7225
PvuII CAG'CTG
Cuts at: 3125 3517 4015 3125
Size: 392 498 6335
Fragments arranged by size:
6335 498 392
SacI G_AGCT'C
Cuts at: 2091 2100 2091
Size: 9 7216
SalI G'TCGA_C
Cuts at: 1936 4567 1936
Size: 2631 4594
SapI GCTCTTCn'nnn_
Cuts at: 3445 3445
Size: 7225
SbfI CC_TGCA'GG
Cuts at: 4577 4577
Size: 7225
ScaI AGT'ACT
Cuts at: 4193 5473 7185 4193
Size: 1280 1712 4233
Fragments arranged by size:
4233 1712 1280
SexAI A'CCwGG_T
Cuts at: 3771 3771
Size: 7225
SfcI C'TryA_G
Cuts at: 826 1045 2858 3512 3874 4016 4104 4573 5708
Size: 219 1813 654 362 142 88 469 1135
Cuts at: 5708 6389 6580 826
Size: 681 191 1471
Fragments arranged by size:
1813 1471 1135 681 654 469 362 219
191 142 88
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7225
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7225
SmaI CCC'GGG
Cuts at: 1872 4552 1872
Size: 2680 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2246 5329 6230 6492 820
Size: 197 926 303 3083 901 262 1553
Fragments arranged by size:
3083 1553 926 901 303 262 197
SnaBI TAC'GTA
Cuts at: 493 5016 493
Size: 4523 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7225
SphI G_CATG'C
Cuts at: 1132 4583 1132
Size: 3451 3774
SspI AAT'ATT
Cuts at: 5 52 3139 5149 5
Size: 47 3087 2010 2081
Fragments arranged by size:
3087 2081 2010 47
StuI AGG'CCT
Cuts at: 1517 2351 1517
Size: 834 6391
StyI C'CwwG_G
Cuts at: 513 1065 2004 2683 3051 4132 4664 513
Size: 552 939 679 368 1081 532 3074
Fragments arranged by size:
3074 1081 939 679 552 532 368
SwaI ATTT'AAAT
Cuts at: 2124 2124
Size: 7225
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2969 5252 5269 5422 5607 6052 325
Size: 1255 1389 2283 17 153 185 445 1498
Fragments arranged by size:
2283 1498 1389 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2595 4191 5471
Size: 274 80 33 51 1443 618 1596 1280
Cuts at: 5471 7183 96
Size: 1712 138
Fragments arranged by size:
1712 1596 1443 1280 618 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7185 7217 7185
Size: 32 7193
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7225
XbaI T'CTAG_A
Cuts at: 2302 4561 2302
Size: 2259 4966
XhoI C'TCGA_G
Cuts at: 1943 2246 1943
Size: 303 6922
XmaI C'CCGG_G
Cuts at: 1870 4550 1870
Size: 2680 4545
XmnI GAAnn'nnTTC
Cuts at: 2652 2666 3335 4451 5354 2652
Size: 14 669 1116 903 4523
Fragments arranged by size:
4523 1116 903 669 14
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2950 3811 4159 5033
Size: 53 83 186 936 1416 861 348 874
Cuts at: 5033 276
Size: 2468
Fragments arranged by size:
2468 1416 936 874 861 348 186 83
53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PpuMI PspXI RsrII SacII SanDI SfiI SfoI
SrfI XcmI