Restriction map for
pFN21AE6755
(Circular) (Six-base) MAPSORT of: pFN21AE6755.seq Check: 9521 from: 1 to: 6364
pFN21AE6755 6364 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4174 278
Size: 53 83 186 936 2638 2468
Fragments arranged by size:
2638 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3444 3707 1937
Size: 1507 263 4594
Fragments arranged by size:
4594 1507 263
Acc65I G'GTAC_C
Cuts at: 3685 3685
Size: 6364
AclI AA'CG_TT
Cuts at: 1563 3338 3663 4491 4864 1563
Size: 1775 325 828 373 3063
Fragments arranged by size:
3063 1775 828 373 325
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2561 3144 3267 4427 5811 6079 1632
Size: 929 583 123 1160 1384 268 1917
Fragments arranged by size:
1917 1384 1160 929 583 268 123
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6167
AflIII A'CryG_T
Cuts at: 1203 2550 2653 5263 6240 1203
Size: 1347 103 2610 977 1327
Fragments arranged by size:
2610 1347 1327 977 103
AgeI A'CCGG_T
Cuts at: 5195 5195
Size: 6364
AhdI GACnn_n'nnGTC
Cuts at: 5093 5093
Size: 6364
AleI CACnn'nnGTG
Cuts at: 1166 2612 1166
Size: 1446 4918
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6364
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2076 2108 2076
Size: 32 6332
AlwNI CAG_nnn'CTG
Cuts at: 4107 5679 4107
Size: 1572 4792
ApaI G_GGCC'C
Cuts at: 2679 3332 2679
Size: 653 5711
ApaLI G'TGCA_C
Cuts at: 4421 5577 4421
Size: 1156 5208
ApoI r'AATT_y
Cuts at: 1493 2863 2888 2967 3108 3673 3899 4051 6160
Size: 1370 25 79 141 565 226 152 2109
Cuts at: 6160 1493
Size: 1697
Fragments arranged by size:
2109 1697 1370 565 226 152 141 79
25
AseI AT'TA_AT
Cuts at: 160 1032 4918 160
Size: 872 3886 1606
Fragments arranged by size:
3886 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6364
AvaI C'yCGr_G
Cuts at: 1870 1943 3689 1870
Size: 73 1746 4545
Fragments arranged by size:
4545 1746 73
BamHI G'GATC_C
Cuts at: 3451 3694 4061 3451
Size: 243 367 5754
Fragments arranged by size:
5754 367 243
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2074 3685 5140 618
Size: 325 217 627 287 1611 1455 1842
Fragments arranged by size:
1842 1611 1455 627 325 287 217
BanII G_rGCy'C
Cuts at: 1391 2014 2679 3261 3332 3540 3683 1391
Size: 623 665 582 71 208 143 4072
Fragments arranged by size:
4072 665 623 582 208 143 71
BbeI G_GCGC'C
Cuts at: 2078 2078
Size: 6364
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2180 2508 3545 928
Size: 971 281 328 1037 3747
Fragments arranged by size:
3747 1037 971 328 281
BbvCI CC'TCA_GC
Cuts at: 3030 3167 3360 3030
Size: 137 193 6034
Fragments arranged by size:
6034 193 137
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4555 4589 1903
Size: 34 50 34 2534 34 3678
Fragments arranged by size:
3678 2534 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3022 4256 5466 3022
Size: 1234 1210 3920
Fragments arranged by size:
3920 1234 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6364
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4975 136
Size: 107 122 71 631 605 337 2966 1525
Fragments arranged by size:
2966 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 6359 6359
Size: 6364
BlpI GC'TnA_GC
Cuts at: 2691 3781 2691
Size: 1090 5274
Bme1580I G_kGCm'C
Cuts at: 1790 2679 3332 4425 5581 1790
Size: 889 653 1093 1156 2573
Fragments arranged by size:
2573 1156 1093 889 653
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6364
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2584 3002 3457
Size: 416 53 83 399 24 1167 418 455
Cuts at: 3457 4095 5053 442
Size: 638 958 1753
Fragments arranged by size:
1753 1167 958 638 455 418 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6364
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3072 3072
Size: 6364
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2460 2648 3567 5024 1239
Size: 1221 188 919 1457 2579
Fragments arranged by size:
2579 1457 1221 919 188
Bpu10I CC'TnA_GC
Cuts at: 3030 3167 3360 3030
Size: 137 193 6034
Fragments arranged by size:
6034 193 137
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4489 5354 5652 4489
Size: 865 298 5201
Fragments arranged by size:
5201 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5027 882
Size: 617 3528 2219
Fragments arranged by size:
3528 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2656 4155 493
Size: 711 1452 1499 2702
Fragments arranged by size:
2702 1499 1452 711
BsaBI GATnn'nnATC
Cuts at: 6358 6358
Size: 6364
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2075 4171 4553 5188
Size: 53 83 186 936 542 2096 382 635
Cuts at: 5188 275
Size: 1451
Fragments arranged by size:
2096 1451 936 635 542 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4797 5195 5469 5616 6027 4797
Size: 398 274 147 411 5134
Fragments arranged by size:
5134 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2187 2217 3263 3293 1178
Size: 30 165 30 784 30 1046 30 4249
Fragments arranged by size:
4249 1046 784 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3083 3287 3422 3560 3587 1186
Size: 1897 204 135 138 27 3963
Fragments arranged by size:
3963 1897 204 138 135 27
BseYI C'CCAG_C
Cuts at: 2857 3035 3153 5567 6063 2857
Size: 178 118 2414 496 3158
Fragments arranged by size:
3158 2414 496 178 118
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2237 3671 1163
Size: 573 501 1434 3856
Fragments arranged by size:
3856 1434 573 501
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2020 2036 3814 4575 5603 1540
Size: 275 186 19 16 1778 761 1028 2301
Fragments arranged by size:
2301 1778 1028 761 275 186 19 16
BsiHKAI G_wGCw'C
Cuts at: 2619 2692 3261 3396 4425 4510 5581 2619
Size: 73 569 135 1029 85 1071 3402
Fragments arranged by size:
3402 1071 1029 569 135 85 73
BsmI GAATG_Cn'
Cuts at: 2267 3875 3968 2267
Size: 1608 93 4663
Fragments arranged by size:
4663 1608 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2014 2619 2679 2692 3261 3332 3396
Size: 399 224 605 60 13 569 71 64
Cuts at: 3396 3540 3683 4425 4510 5581 1391
Size: 144 143 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 605 569 399 224 144
143 85 71 64 60 13
BspEI T'CCGG_A
Cuts at: 6027 6027
Size: 6364
BspHI T'CATG_A
Cuts at: 4253 5983 4253
Size: 1730 4634
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2131 3719 844
Size: 1287 1588 3489
Fragments arranged by size:
3489 1588 1287
BsrBI CCG'CTC
Cuts at: 1394 1823 4251 1394
Size: 429 2428 3507
Fragments arranged by size:
3507 2428 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4859 5033 65
Size: 1183 3611 174 1396
Fragments arranged by size:
3611 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3577 5008 5195 1700
Size: 110 1767 1431 187 2869
Fragments arranged by size:
2869 1767 1431 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6364
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6364
BssSI C'ACGA_G
Cuts at: 4424 5436 4424
Size: 1012 5352
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6364
BstBI TT'CG_AA
Cuts at: 5168 5168
Size: 6364
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6364
BstXI CCAn_nnnn'nTGG
Cuts at: 2484 2484
Size: 6364
BstYI r'GATC_y
Cuts at: 1972 3451 3694 4061 4446 4463 5915 6001 6013
Size: 1479 243 367 385 17 1452 86 12
Cuts at: 6013 6359 1972
Size: 346 1977
Fragments arranged by size:
1977 1479 1452 385 367 346 243 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3047 3057 3047
Size: 10 6354
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5812
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5625
Fragments arranged by size:
5625 683 56
BtsI GCAGTG_nn'
Cuts at: 2714 3010 3880 4674 4694 2714
Size: 296 870 794 20 4384
Fragments arranged by size:
4384 870 794 296 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6364
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6329
DraI TTT'AAA
Cuts at: 3668 3668
Size: 6364
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5371 809
Size: 507 4055 1802
Fragments arranged by size:
4055 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2017 2033 3811
Size: 54 1425 50 275 18 187 16 1778
Cuts at: 3811 4700 6329 8
Size: 889 1629 43
Fragments arranged by size:
1778 1629 1425 889 275 187 54 50
43 18 16
EagI C'GGCC_G
Cuts at: 1537 1812 2017 2033 3811 1537
Size: 275 205 16 1778 4090
Fragments arranged by size:
4090 1778 275 205 16
EarI CTCTTCn'nnn_
Cuts at: 1353 2483 3060 3243 3417 4294 1353
Size: 1130 577 183 174 877 3423
Fragments arranged by size:
3423 1130 877 577 183 174
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4951 5325 5471 387
Size: 1064 138 3362 374 146 1280
Fragments arranged by size:
3362 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3259 3259
Size: 6364
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2460 2561 2648 3144 3267 3567 4427
Size: 393 828 101 87 496 123 300 860
Cuts at: 4427 5024 5811 6079 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 860 828 787 597 496 393 300
268 123 101 87
EcoNI CCTnn'n_nnAGG
Cuts at: 3386 3386
Size: 6364
EcoO109I rG'GnC_Cy
Cuts at: 2675 2676 3051 3242 2675
Size: 1 375 191 5797
Fragments arranged by size:
5797 375 191 1
EcoRI G'AATT_C
Cuts at: 3673 4051 3673
Size: 378 5986
FspI TGC'GCA
Cuts at: 4870 4870
Size: 6364
HaeII r_GCGC'y
Cuts at: 1678 2078 5511 1678
Size: 400 3433 2531
Fragments arranged by size:
3433 2531 400
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2435 2467 2720 2752 3263
Size: 32 135 32 863 32 253 32 511
Cuts at: 3263 3295 5005 5037 5079 5111 1373
Size: 32 1710 32 42 32 2626
Fragments arranged by size:
2626 1710 863 511 253 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3445 3708 3954 669
Size: 988 281 1507 263 246 3079
Fragments arranged by size:
3079 1507 988 281 263 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6364
HpaI GTT'AAC
Cuts at: 3954 3954
Size: 6364
KasI G'GCGC_C
Cuts at: 2074 2074
Size: 6364
KpnI G_GTAC'C
Cuts at: 3689 3689
Size: 6364
MfeI C'AATT_G
Cuts at: 3963 3963
Size: 6364
MluI A'CGCG_T
Cuts at: 6240 6240
Size: 6364
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5478 5662 1305
Size: 4173 184 2007
Fragments arranged by size:
4173 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4542
Fragments arranged by size:
4542 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2150 2612 4322 4681 4840 518
Size: 648 984 462 1710 359 159 2042
Fragments arranged by size:
2042 1710 984 648 462 359 159
MspA1I CmG'CkG
Cuts at: 1606 2038 2044 2053 3039 3780 4457 5605 5850
Size: 432 6 9 986 741 677 1148 245
Cuts at: 5850 1606
Size: 2120
Fragments arranged by size:
2120 1148 986 741 677 432 245 9
6
NaeI GCC'GGC
Cuts at: 3579 3579
Size: 6364
NarI GG'CG_CC
Cuts at: 2075 2075
Size: 6364
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5812
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6364
NgoMIV G'CCGG_C
Cuts at: 3577 3577
Size: 6364
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6364
NotI GC'GGCC_GC
Cuts at: 2033 3811 2033
Size: 1778 4586
NspI r_CATG'y
Cuts at: 1132 1229 3722 5267 1132
Size: 97 2493 1545 2229
Fragments arranged by size:
2493 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2672 1348
Size: 1324 5040
PciI A'CATG_T
Cuts at: 5263 5263
Size: 6364
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2611 1108
Size: 1503 4861
PmeI GTTT'AAAC
Cuts at: 3668 3668
Size: 6364
PmlI CAC'GTG
Cuts at: 2656 2656
Size: 6364
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4433 4465 5946 5978 4433
Size: 32 1481 32 4819
Fragments arranged by size:
4819 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3440 1613
Size: 1827 4537
PsiI TTA'TAA
Cuts at: 3934 3934
Size: 6364
PspOMI G'GGCC_C
Cuts at: 2675 3328 2675
Size: 653 5711
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2627 2659 2627
Size: 32 6332
PstI C_TGCA'G
Cuts at: 830 3716 830
Size: 2886 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6364
SacI G_AGCT'C
Cuts at: 3261 3261
Size: 6364
SalI G'TCGA_C
Cuts at: 1936 3443 3706 1936
Size: 1507 263 4594
Fragments arranged by size:
4594 1507 263
SapI GCTCTTCn'nnn_
Cuts at: 3243 3417 3243
Size: 174 6190
SbfI CC_TGCA'GG
Cuts at: 3716 3716
Size: 6364
ScaI AGT'ACT
Cuts at: 3347 4612 6324 3347
Size: 1265 1712 3387
Fragments arranged by size:
3387 1712 1265
SfcI C'TryA_G
Cuts at: 826 1045 2439 3712 4847 5528 5719 826
Size: 219 1394 1273 1135 681 191 1471
Fragments arranged by size:
1471 1394 1273 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2076 2076
Size: 6364
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6364
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6364
SmaI CCC'GGG
Cuts at: 1872 3691 1872
Size: 1819 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4468 5369 5631 820
Size: 197 926 2525 901 262 1553
Fragments arranged by size:
2525 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4155 493
Size: 3662 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6364
SphI G_CATG'C
Cuts at: 1132 3722 1132
Size: 2590 3774
SspI AAT'ATT
Cuts at: 5 52 4288 5
Size: 47 4236 2081
Fragments arranged by size:
4236 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6364
StyI C'CwwG_G
Cuts at: 513 1065 2078 3803 513
Size: 552 1013 1725 3074
Fragments arranged by size:
3074 1725 1013 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2871 3167 4391 4408 4561 4746 5191
Size: 1255 1291 296 1224 17 153 185 445
Cuts at: 5191 325
Size: 1498
Fragments arranged by size:
1498 1291 1255 1224 445 296 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3345 4610 6322
Size: 274 80 33 51 1443 1368 1265 1712
Cuts at: 6322 96
Size: 138
Fragments arranged by size:
1712 1443 1368 1265 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2645 2677 6324 6356 2645
Size: 32 3647 32 2653
Fragments arranged by size:
3647 2653 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6364
XbaI T'CTAG_A
Cuts at: 3560 3700 3560
Size: 140 6224
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2608 2608
Size: 6364
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6364
XmaI C'CCGG_G
Cuts at: 1870 3689 1870
Size: 1819 4545
XmnI GAAnn'nnTTC
Cuts at: 2505 4493 2505
Size: 1988 4376
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4172 276
Size: 53 83 186 936 2638 2468
Fragments arranged by size:
2638 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspOMI PsrI PstI PvuI SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstZ17I DraIII EcoRV FalI FseI FspAI NruI NsiI
PacI PfoI PpuMI PspXI PvuII RsrII SacII SanDI
SexAI SfiI SrfI SwaI