Restriction map for
pFN21AE6754
(Circular) (Six-base) MAPSORT of: pFN21AE6754.seq Check: 962 from: 1 to: 6847
pFN21AE6754 6847 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2393 2516 3309 2393
Size: 123 793 5931
Fragments arranged by size:
5931 793 123
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4657 278
Size: 53 83 186 936 3121 2468
Fragments arranged by size:
3121 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2322 3851 4190 1937
Size: 385 1529 339 4594
Fragments arranged by size:
4594 1529 385 339
Acc65I G'GTAC_C
Cuts at: 4168 4168
Size: 6847
AclI AA'CG_TT
Cuts at: 1563 3147 4974 5347 1563
Size: 1584 1827 373 3063
Fragments arranged by size:
3063 1827 1584 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3740 3925 4910 6294 6562 1632
Size: 2108 185 985 1384 268 1917
Fragments arranged by size:
2108 1917 1384 985 268 185
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6650
AflIII A'CryG_T
Cuts at: 1203 3777 5746 6723 1203
Size: 2574 1969 977 1327
Fragments arranged by size:
2574 1969 1327 977
AgeI A'CCGG_T
Cuts at: 5678 5678
Size: 6847
AhdI GACnn_n'nnGTC
Cuts at: 5576 5576
Size: 6847
AleI CACnn'nnGTG
Cuts at: 1166 2213 1166
Size: 1047 5800
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3033 4007 1184
Size: 1849 974 4024
Fragments arranged by size:
4024 1849 974
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2123 2155 2123
Size: 32 6815
AlwNI CAG_nnn'CTG
Cuts at: 2557 4590 6162 2557
Size: 2033 1572 3242
Fragments arranged by size:
3242 2033 1572
ApaLI G'TGCA_C
Cuts at: 4904 6060 4904
Size: 1156 5691
ApoI r'AATT_y
Cuts at: 1493 2481 3374 4156 4382 4534 6643 1493
Size: 988 893 782 226 152 2109 1697
Fragments arranged by size:
2109 1697 988 893 782 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5401 160
Size: 872 4369 1606
Fragments arranged by size:
4369 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6847
AvaI C'yCGr_G
Cuts at: 1870 1943 2456 2689 2877 4089 4172 1870
Size: 73 513 233 188 1212 83 4545
Fragments arranged by size:
4545 1212 513 233 188 83 73
BamHI G'GATC_C
Cuts at: 4177 4544 4177
Size: 367 6480
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2363 2405 2864 3247 4021
Size: 325 217 627 576 42 459 383 774
Cuts at: 4021 4168 5623 618
Size: 147 1455 1842
Fragments arranged by size:
1842 1455 774 627 576 459 383 325
217 147 42
BanII G_rGCy'C
Cuts at: 1391 2034 2807 2998 4166 1391
Size: 643 773 191 1168 4072
Fragments arranged by size:
4072 1168 773 643 191
BbeI G_GCGC'C
Cuts at: 2868 4025 2868
Size: 1157 5690
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2093 2839 2845 928
Size: 971 194 746 6 4930
Fragments arranged by size:
4930 971 746 194 6
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2269 2287 2303 2321 2503 2537 5038
Size: 34 332 18 16 18 182 34 2501
Cuts at: 5038 5072 1903
Size: 34 3678
Fragments arranged by size:
3678 2501 332 182 34 34 34 18
18 16
BciVI GTATCCnnnnn_n'
Cuts at: 2929 4739 5949 2929
Size: 1810 1210 3827
Fragments arranged by size:
3827 1810 1210
BclI T'GATC_A
Cuts at: 1548 2154 1548
Size: 606 6241
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5458 136
Size: 107 122 71 631 605 3786 1525
Fragments arranged by size:
3786 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3558 6842 3558
Size: 3284 3563
BlpI GC'TnA_GC
Cuts at: 4264 4264
Size: 6847
Bme1580I G_kGCm'C
Cuts at: 1790 3365 4908 6064 1790
Size: 1575 1543 1156 2573
Fragments arranged by size:
2573 1575 1543 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6847
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3315 4578 5536
Size: 416 53 83 399 24 1898 1263 958
Cuts at: 5536 442
Size: 1753
Fragments arranged by size:
1898 1753 1263 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6847
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2534 3386 3769 5507 1239
Size: 1295 852 383 1738 2579
Fragments arranged by size:
2579 1738 1295 852 383
Bpu10I CC'TnA_GC
Cuts at: 3252 3263 3675 4035 3252
Size: 11 412 360 6064
Fragments arranged by size:
6064 412 360 11
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3440 4972 5837 6135 3440
Size: 1532 865 298 4152
Fragments arranged by size:
4152 1532 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3799 3969 5510 882
Size: 617 2300 170 1541 2219
Fragments arranged by size:
2300 2219 1541 617 170
BsaAI yAC'GTr
Cuts at: 493 1204 4638 493
Size: 711 3434 2702
Fragments arranged by size:
3434 2702 711
BsaBI GATnn'nnATC
Cuts at: 2159 6841 2159
Size: 4682 2165
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2286 2319 2865 4022
Size: 53 83 186 936 753 33 546 1157
Cuts at: 4022 4654 5036 5671 275
Size: 632 382 635 1451
Fragments arranged by size:
1451 1157 936 753 635 632 546 382
186 83 53 33
BsaWI w'CCGG_w
Cuts at: 3951 5280 5678 5952 6099 6510 3951
Size: 1329 398 274 147 411 4288
Fragments arranged by size:
4288 1329 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4091 4094 4121 4124 1178
Size: 30 165 30 2688 3 27 3 3901
Fragments arranged by size:
3901 2688 165 30 30 27 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2326 3389 4099 1186
Size: 1140 1063 710 3934
Fragments arranged by size:
3934 1140 1063 710
BseYI C'CCAG_C
Cuts at: 2049 2067 2073 2091 2449 2509 2728 6050 6546
Size: 18 6 18 358 60 219 3322 496
Cuts at: 6546 2049
Size: 2350
Fragments arranged by size:
3322 2350 496 358 219 60 18 18
6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2045 2857 3024 1163
Size: 573 309 812 167 4986
Fragments arranged by size:
4986 812 573 309 167
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4297 5058 6086 1540
Size: 275 186 2296 761 1028 2301
Fragments arranged by size:
2301 2296 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2034 2178 2220 2998 3264 3300 4908 4993 6064
Size: 144 42 778 266 36 1608 85 1071
Cuts at: 6064 2034
Size: 2817
Fragments arranged by size:
2817 1608 1071 778 266 144 85 42
36
BsmI GAATG_Cn'
Cuts at: 2793 4078 4358 4451 2793
Size: 1285 280 93 5189
Fragments arranged by size:
5189 1285 280 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2034 2178 2220 2807 2998 3264 3300
Size: 399 244 144 42 587 191 266 36
Cuts at: 3300 3365 4166 4908 4993 6064 1391
Size: 65 801 742 85 1071 2174
Fragments arranged by size:
2174 1071 801 742 587 399 266 244
191 144 85 65 42 36
BspEI T'CCGG_A
Cuts at: 6510 6510
Size: 6847
BspHI T'CATG_A
Cuts at: 4736 6466 4736
Size: 1730 5117
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2393 2516 3309 3349 4202 844
Size: 1549 123 793 40 853 3489
Fragments arranged by size:
3489 1549 853 793 123 40
BsrBI CCG'CTC
Cuts at: 1394 1823 4734 1394
Size: 429 2911 3507
Fragments arranged by size:
3507 2911 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3015 5342 5516 65
Size: 1183 1767 2327 174 1396
Fragments arranged by size:
2327 1767 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2420 2750 3256 5491 5678 1700
Size: 110 610 330 506 2235 187 2869
Fragments arranged by size:
2869 2235 610 506 330 187 110
BsrGI T'GTAC_A
Cuts at: 96 2838 96
Size: 2742 4105
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6847
BssSI C'ACGA_G
Cuts at: 2827 4115 4907 5919 2827
Size: 1288 792 1012 3755
Fragments arranged by size:
3755 1288 1012 792
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4135 794
Size: 3341 3506
BstBI TT'CG_AA
Cuts at: 5651 5651
Size: 6847
BstEII G'GTnAC_C
Cuts at: 1184 3112 1184
Size: 1928 4919
BstXI CCAn_nnnn'nTGG
Cuts at: 3388 3388
Size: 6847
BstYI r'GATC_y
Cuts at: 1972 3558 3755 4177 4544 4929 4946 6398 6484
Size: 1586 197 422 367 385 17 1452 86
Cuts at: 6484 6496 6842 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1586 1452 422 385 367 346 197
86 17 12
BstZ17I GTA'TAC
Cuts at: 3852 3852
Size: 6847
Bsu36I CC'TnA_GG
Cuts at: 2183 2774 3645 2183
Size: 591 871 5385
Fragments arranged by size:
5385 871 591
BtgI C'CryG_G
Cuts at: 513 1065 3084 3117 513
Size: 552 2019 33 4243
Fragments arranged by size:
4243 2019 552 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6108
Fragments arranged by size:
6108 683 56
BtsI GCAGTG_nn'
Cuts at: 3017 3201 3286 4363 5157 5177 3017
Size: 184 85 1077 794 20 4687
Fragments arranged by size:
4687 1077 794 184 85 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6847
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6812
DraI TTT'AAA
Cuts at: 4151 4151
Size: 6847
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2014 5854 809
Size: 507 698 3840 1802
Fragments arranged by size:
3840 1802 698 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2574 2748 3898
Size: 54 1425 50 275 18 744 174 1150
Cuts at: 3898 4294 5183 6812 8
Size: 396 889 1629 43
Fragments arranged by size:
1629 1425 1150 889 744 396 275 174
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4294 1537
Size: 275 2482 4090
Fragments arranged by size:
4090 2482 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2039 2183 3467 4777 1353
Size: 686 144 1284 1310 3423
Fragments arranged by size:
3423 1310 1284 686 144
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2534 5434 5808 5954 387
Size: 1064 138 945 2900 374 146 1280
Fragments arranged by size:
2900 1280 1064 945 374 146 138
EcoICRI GAG'CTC
Cuts at: 2032 2996 2032
Size: 964 5883
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2534 3386 3740 3769 3925 4910 5507
Size: 393 902 852 354 29 156 985 597
Cuts at: 5507 6294 6562 1239
Size: 787 268 1524
Fragments arranged by size:
1524 985 902 852 787 597 393 354
268 156 29
EcoNI CCTnn'n_nnAGG
Cuts at: 3982 4034 3982
Size: 52 6795
EcoO109I rG'GnC_Cy
Cuts at: 2124 2884 3338 3456 3816 2124
Size: 760 454 118 360 5155
Fragments arranged by size:
5155 760 454 360 118
EcoRI G'AATT_C
Cuts at: 3374 4156 4534 3374
Size: 782 378 5687
Fragments arranged by size:
5687 782 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3777 3777
Size: 6847
FspI TGC'GCA
Cuts at: 5353 5353
Size: 6847
HaeII r_GCGC'y
Cuts at: 1678 2868 4025 5994 1678
Size: 1190 1157 1969 2531
Fragments arranged by size:
2531 1969 1190 1157
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2352 2384 3518 3550 3949
Size: 32 135 32 780 32 1134 32 399
Cuts at: 3949 3981 5488 5520 5562 5594 1373
Size: 32 1507 32 42 32 2626
Fragments arranged by size:
2626 1507 1134 780 399 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3862 4042 4191 4437 669
Size: 988 281 1924 180 149 246 3079
Fragments arranged by size:
3079 1924 988 281 246 180 149
HindIII A'AGCT_T
Cuts at: 748 3416 748
Size: 2668 4179
HpaI GTT'AAC
Cuts at: 3862 4437 3862
Size: 575 6272
KasI G'GCGC_C
Cuts at: 2864 4021 2864
Size: 1157 5690
KpnI G_GTAC'C
Cuts at: 4172 4172
Size: 6847
MfeI C'AATT_G
Cuts at: 4446 4446
Size: 6847
MluI A'CGCG_T
Cuts at: 6723 6723
Size: 6847
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3960 5961 6145 1305
Size: 2655 2001 184 2007
Fragments arranged by size:
2655 2007 2001 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5025
Fragments arranged by size:
5025 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2213 2252 3833 4049 4805 5164 5323
Size: 648 1047 39 1581 216 756 359 159
Cuts at: 5323 518
Size: 2042
Fragments arranged by size:
2042 1581 1047 756 648 359 216 159
39
MspA1I CmG'CkG
Cuts at: 1606 2095 2313 2554 2732 2816 3578 4132 4263
Size: 489 218 241 178 84 762 554 131
Cuts at: 4263 4940 6088 6333 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 762 677 554 489 245 241
218 178 131 84
NaeI GCC'GGC
Cuts at: 2752 2752
Size: 6847
NarI GG'CG_CC
Cuts at: 2865 4022 2865
Size: 1157 5690
NcoI C'CATG_G
Cuts at: 513 1065 3117 513
Size: 552 2052 4243
Fragments arranged by size:
4243 2052 552
NdeI CA'TA_TG
Cuts at: 387 3655 387
Size: 3268 3579
NgoMIV G'CCGG_C
Cuts at: 2750 2750
Size: 6847
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6847
NotI GC'GGCC_GC
Cuts at: 4294 4294
Size: 6847
NspI r_CATG'y
Cuts at: 1132 1229 2230 3781 4205 5750 1132
Size: 97 1001 1551 424 1545 2229
Fragments arranged by size:
2229 1551 1545 1001 424 97
PasI CC'CwG_GG
Cuts at: 1348 2800 3789 1348
Size: 1452 989 4406
Fragments arranged by size:
4406 1452 989
PciI A'CATG_T
Cuts at: 3777 5746 3777
Size: 1969 4878
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2630 1108
Size: 1522 5325
PmeI GTTT'AAAC
Cuts at: 4151 4151
Size: 6847
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4916 4948 6429 6461 4916
Size: 32 1481 32 5302
Fragments arranged by size:
5302 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2124 3338 3456 3816 2124
Size: 1214 118 360 5155
Fragments arranged by size:
5155 1214 360 118
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6847
PsiI TTA'TAA
Cuts at: 4417 4417
Size: 6847
PstI C_TGCA'G
Cuts at: 830 3012 3236 3986 4199 830
Size: 2182 224 750 213 3478
Fragments arranged by size:
3478 2182 750 224 213
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6847
PvuII CAG'CTG
Cuts at: 2095 2554 2816 4132 2095
Size: 459 262 1316 4810
Fragments arranged by size:
4810 1316 459 262
SacI G_AGCT'C
Cuts at: 2034 2998 2034
Size: 964 5883
SalI G'TCGA_C
Cuts at: 1936 4189 1936
Size: 2253 4594
SanDI GG'GwC_CC
Cuts at: 3816 3816
Size: 6847
SapI GCTCTTCn'nnn_
Cuts at: 2039 2183 2039
Size: 144 6703
SbfI CC_TGCA'GG
Cuts at: 3986 4199 3986
Size: 213 6634
ScaI AGT'ACT
Cuts at: 5095 6807 5095
Size: 1712 5135
SfcI C'TryA_G
Cuts at: 826 1045 2660 2736 3008 3232 3982 4195 5330
Size: 219 1615 76 272 224 750 213 1135
Cuts at: 5330 6011 6202 826
Size: 681 191 1471
Fragments arranged by size:
1615 1471 1135 750 681 272 224 219
213 191 76
SfoI GGC'GCC
Cuts at: 2866 4023 2866
Size: 1157 5690
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6847
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6847
SmaI CCC'GGG
Cuts at: 1872 2691 4174 1872
Size: 819 1483 4545
Fragments arranged by size:
4545 1483 819
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2877 3419 4951 5852 6114 820
Size: 197 926 934 542 1532 901 262 1553
Fragments arranged by size:
1553 1532 934 926 901 542 262 197
SnaBI TAC'GTA
Cuts at: 493 4638 493
Size: 4145 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6847
SphI G_CATG'C
Cuts at: 1132 4205 1132
Size: 3073 3774
SspI AAT'ATT
Cuts at: 5 52 4771 5
Size: 47 4719 2081
Fragments arranged by size:
4719 2081 47
StuI AGG'CCT
Cuts at: 1517 3282 1517
Size: 1765 5082
StyI C'CwwG_G
Cuts at: 513 1065 3117 3381 3922 4286 513
Size: 552 2052 264 541 364 3074
Fragments arranged by size:
3074 2052 552 541 364 264
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2329 2336 2627 3835 3857 4079 4874
Size: 1255 749 7 291 1208 22 222 795
Cuts at: 4874 4891 5044 5229 5674 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1208 795 749 445 291 222
185 153 22 17 7
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2838 5093 6805
Size: 274 80 33 51 1443 861 2255 1712
Cuts at: 6805 96
Size: 138
Fragments arranged by size:
2255 1712 1443 861 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4094 4126 6807 6839 4094
Size: 32 2681 32 4102
Fragments arranged by size:
4102 2681 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6847
XbaI T'CTAG_A
Cuts at: 4183 4183
Size: 6847
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2797 2797
Size: 6847
XhoI C'TCGA_G
Cuts at: 1943 2877 1943
Size: 934 5913
XmaI C'CCGG_G
Cuts at: 1870 2689 4172 1870
Size: 819 1483 4545
Fragments arranged by size:
4545 1483 819
XmnI GAAnn'nnTTC
Cuts at: 4976 4976
Size: 6847
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4655 276
Size: 53 83 186 936 3121 2468
Fragments arranged by size:
3121 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BsmBI DraIII EcoRV FseI FspAI NruI NsiI
PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII
SexAI SfiI SrfI SwaI