Restriction map for
pFN21AE6753
(Circular) (Six-base) MAPSORT of: pFN21AE6753.seq Check: 8296 from: 1 to: 6712
pFN21AE6753 6712 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4522 278
Size: 53 83 186 936 2986 2468
Fragments arranged by size:
2986 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4055 1937
Size: 2118 4594
Acc65I G'GTAC_C
Cuts at: 4033 4033
Size: 6712
AclI AA'CG_TT
Cuts at: 1563 4011 4839 5212 1563
Size: 2448 828 373 3063
Fragments arranged by size:
3063 2448 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2010 3477 3551 4775 6159 6427 1632
Size: 378 1467 74 1224 1384 268 1917
Fragments arranged by size:
1917 1467 1384 1224 378 268 74
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6515
AflIII A'CryG_T
Cuts at: 1203 3612 5611 6588 1203
Size: 2409 1999 977 1327
Fragments arranged by size:
2409 1999 1327 977
AgeI A'CCGG_T
Cuts at: 5543 5543
Size: 6712
AhdI GACnn_n'nnGTC
Cuts at: 5441 5441
Size: 6712
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6712
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2296 1184
Size: 1112 5600
AlwNI CAG_nnn'CTG
Cuts at: 2155 3176 3544 4455 6027 2155
Size: 1021 368 911 1572 2840
Fragments arranged by size:
2840 1572 1021 911 368
ApaLI G'TGCA_C
Cuts at: 4769 5925 4769
Size: 1156 5556
ApoI r'AATT_y
Cuts at: 1493 2972 3000 3190 3212 3266 3323 4021 4247
Size: 1479 28 190 22 54 57 698 226
Cuts at: 4247 4399 6508 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1479 698 226 190 152 57
54 28 22
AseI AT'TA_AT
Cuts at: 160 1032 5266 160
Size: 872 4234 1606
Fragments arranged by size:
4234 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6712
AvaI C'yCGr_G
Cuts at: 1870 1943 2567 4037 1870
Size: 73 624 1470 4545
Fragments arranged by size:
4545 1470 624 73
BamHI G'GATC_C
Cuts at: 2303 2537 4042 4409 2303
Size: 234 1505 367 4606
Fragments arranged by size:
4606 1505 367 234
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3146 3450 4033 5488 618
Size: 325 217 627 1359 304 583 1455 1842
Fragments arranged by size:
1842 1455 1359 627 583 325 304 217
BanII G_rGCy'C
Cuts at: 1391 3362 3773 4031 1391
Size: 1971 411 258 4072
Fragments arranged by size:
4072 1971 411 258
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2660 928
Size: 971 761 4980
Fragments arranged by size:
4980 971 761
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4903 4937 1903
Size: 34 2966 34 3678
Fragments arranged by size:
3678 2966 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4604 5814 4604
Size: 1210 5502
BclI T'GATC_A
Cuts at: 1548 2601 1548
Size: 1053 5659
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5323 136
Size: 107 122 71 631 605 3651 1525
Fragments arranged by size:
3651 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6707 6707
Size: 6712
BlpI GC'TnA_GC
Cuts at: 2772 3534 4129 2772
Size: 762 595 5355
Fragments arranged by size:
5355 762 595
Bme1580I G_kGCm'C
Cuts at: 1790 4773 5929 1790
Size: 2983 1156 2573
Fragments arranged by size:
2983 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6712
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2309 2775 4443
Size: 416 53 83 399 24 892 466 1668
Cuts at: 4443 5401 442
Size: 958 1753
Fragments arranged by size:
1753 1668 958 892 466 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6712
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2144 2531 2881 3588 3777 5372 1239
Size: 905 387 350 707 189 1595 2579
Fragments arranged by size:
2579 1595 905 707 387 350 189
Bpu10I CC'TnA_GC
Cuts at: 2090 3455 3767 2090
Size: 1365 312 5035
Fragments arranged by size:
5035 1365 312
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4837 5702 6000 4837
Size: 865 298 5549
Fragments arranged by size:
5549 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2027 2834 2877 5375 882
Size: 617 528 807 43 2498 2219
Fragments arranged by size:
2498 2219 807 617 528 43
BsaAI yAC'GTr
Cuts at: 493 1204 4503 493
Size: 711 3299 2702
Fragments arranged by size:
3299 2702 711
BsaBI GATnn'nnATC
Cuts at: 3821 6706 3821
Size: 2885 3827
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4519 4901 5536 275
Size: 53 83 186 936 2986 382 635 1451
Fragments arranged by size:
2986 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2388 5145 5543 5817 5964 6375 2388
Size: 2757 398 274 147 411 2725
Fragments arranged by size:
2757 2725 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3586 3602 3616 3632 1178
Size: 30 165 30 2183 16 14 16 4258
Fragments arranged by size:
4258 2183 165 30 30 16 16 14
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2211 3701 1186
Size: 1025 1490 4197
Fragments arranged by size:
4197 1490 1025
BseYI C'CCAG_C
Cuts at: 2796 2832 3561 3890 5915 6411 2796
Size: 36 729 329 2025 496 3097
Fragments arranged by size:
3097 2025 729 496 329 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2537 2603 1163
Size: 573 801 66 5272
Fragments arranged by size:
5272 801 573 66
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4162 4923 5951 1540
Size: 275 186 2161 761 1028 2301
Fragments arranged by size:
2301 2161 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3773 4773 4858 5929 3773
Size: 1000 85 1071 4556
Fragments arranged by size:
4556 1071 1000 85
BsmI GAATG_Cn'
Cuts at: 4223 4316 4223
Size: 93 6619
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3362 3773 4031 4773 4858 5929 1391
Size: 399 1572 411 258 742 85 1071 2174
Fragments arranged by size:
2174 1572 1071 742 411 399 258 85
BspEI T'CCGG_A
Cuts at: 6375 6375
Size: 6712
BspHI T'CATG_A
Cuts at: 4601 6331 4601
Size: 1730 4982
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4067 844
Size: 3223 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4599 1394
Size: 429 2776 3507
Fragments arranged by size:
3507 2776 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2405 5207 5381 65
Size: 1183 1157 2802 174 1396
Fragments arranged by size:
2802 1396 1183 1157 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5356 5543 1700
Size: 110 3546 187 2869
Fragments arranged by size:
3546 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4005 96
Size: 3909 2803
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6712
BssSI C'ACGA_G
Cuts at: 4772 5784 4772
Size: 1012 5700
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3206 794
Size: 2412 4300
BstBI TT'CG_AA
Cuts at: 5516 5516
Size: 6712
BstEII G'GTnAC_C
Cuts at: 1184 3847 1184
Size: 2663 4049
BstXI CCAn_nnnn'nTGG
Cuts at: 2372 2372
Size: 6712
BstYI r'GATC_y
Cuts at: 1972 2203 2303 2337 2537 3816 4042 4409 4794
Size: 231 100 34 200 1279 226 367 385
Cuts at: 4794 4811 6263 6349 6361 6707 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1279 385 367 346 231 226
200 100 86 34 17 12
Bsu36I CC'TnA_GG
Cuts at: 3854 3854
Size: 6712
BtgI C'CryG_G
Cuts at: 513 1065 2004 2900 3019 3924 513
Size: 552 939 896 119 905 3301
Fragments arranged by size:
3301 939 905 896 552 119
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2738 2962 487
Size: 683 56 1512 224 4237
Fragments arranged by size:
4237 1512 683 224 56
BtsI GCAGTG_nn'
Cuts at: 2083 2655 3084 4228 5022 5042 2083
Size: 572 429 1144 794 20 3753
Fragments arranged by size:
3753 1144 794 572 429 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6712
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6677
DraI TTT'AAA
Cuts at: 4016 4016
Size: 6712
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5719 809
Size: 507 4403 1802
Fragments arranged by size:
4403 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2195 2404 4159
Size: 54 1425 50 275 18 365 209 1755
Cuts at: 4159 5048 6677 8
Size: 889 1629 43
Fragments arranged by size:
1755 1629 1425 889 365 275 209 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4159 1537
Size: 275 2347 4090
Fragments arranged by size:
4090 2347 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2629 4642 1353
Size: 1276 2013 3423
Fragments arranged by size:
3423 2013 1276
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3317 5299 5673 5819 387
Size: 1064 138 1728 1982 374 146 1280
Fragments arranged by size:
1982 1728 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3771 3771
Size: 6712
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2010 2144 2531 2881 3477 3551 3588
Size: 393 378 134 387 350 596 74 37
Cuts at: 3588 3777 4775 5372 6159 6427 1239
Size: 189 998 597 787 268 1524
Fragments arranged by size:
1524 998 787 597 596 393 387 378
350 268 189 134 74 37
EcoNI CCTnn'n_nnAGG
Cuts at: 2642 3950 2642
Size: 1308 5404
EcoO109I rG'GnC_Cy
Cuts at: 2216 3095 3571 3599 2216
Size: 879 476 28 5329
Fragments arranged by size:
5329 879 476 28
EcoRI G'AATT_C
Cuts at: 4021 4399 4021
Size: 378 6334
FspI TGC'GCA
Cuts at: 5218 5218
Size: 6712
HaeII r_GCGC'y
Cuts at: 1678 2860 3712 5859 1678
Size: 1182 852 2147 2531
Fragments arranged by size:
2531 2147 1182 852
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2241 2273 2586 2618 2938
Size: 32 135 32 669 32 313 32 320
Cuts at: 2938 2970 3337 3369 3602 3634 5353 5385 5427
Size: 32 367 32 233 32 1719 32 42
Cuts at: 5427 5459 1373
Size: 32 2626
Fragments arranged by size:
2626 1719 669 367 320 313 233 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4056 4302 669
Size: 988 281 2118 246 3079
Fragments arranged by size:
3079 2118 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2353 748
Size: 1605 5107
HpaI GTT'AAC
Cuts at: 4302 4302
Size: 6712
KpnI G_GTAC'C
Cuts at: 4037 4037
Size: 6712
MfeI C'AATT_G
Cuts at: 4311 4311
Size: 6712
MluI A'CGCG_T
Cuts at: 6588 6588
Size: 6712
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2284 2989 5826 6010 1305
Size: 979 705 2837 184 2007
Fragments arranged by size:
2837 2007 979 705 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2406 10
Size: 54 1425 343 574 4316
Fragments arranged by size:
4316 1425 574 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2367 2981 4670 5029 5188 518
Size: 648 1201 614 1689 359 159 2042
Fragments arranged by size:
2042 1689 1201 648 614 359 159
MspA1I CmG'CkG
Cuts at: 1606 2276 2902 3253 3404 4128 4805 5953 6198
Size: 670 626 351 151 724 677 1148 245
Cuts at: 6198 1606
Size: 2120
Fragments arranged by size:
2120 1148 724 677 670 626 351 245
151
NcoI C'CATG_G
Cuts at: 513 1065 2004 3019 3924 513
Size: 552 939 1015 905 3301
Fragments arranged by size:
3301 1015 939 905 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6712
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6712
NotI GC'GGCC_GC
Cuts at: 4159 4159
Size: 6712
NspI r_CATG'y
Cuts at: 1132 1229 3616 3731 4070 5615 1132
Size: 97 2387 115 339 1545 2229
Fragments arranged by size:
2387 2229 1545 339 115 97
PasI CC'CwG_GG
Cuts at: 1348 2826 3362 1348
Size: 1478 536 4698
Fragments arranged by size:
4698 1478 536
PciI A'CATG_T
Cuts at: 3612 5611 3612
Size: 1999 4713
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2413 1108
Size: 1305 5407
PfoI T'CCnGG_A
Cuts at: 2160 2232 3258 2160
Size: 72 1026 5614
Fragments arranged by size:
5614 1026 72
PmeI GTTT'AAAC
Cuts at: 4016 4016
Size: 6712
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4781 4813 6294 6326 4781
Size: 32 1481 32 5167
Fragments arranged by size:
5167 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2216 3095 3599 2216
Size: 879 504 5329
Fragments arranged by size:
5329 879 504
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6712
PsiI TTA'TAA
Cuts at: 4282 4282
Size: 6712
PspXI vC'TCGA_Gb
Cuts at: 2567 2567
Size: 6712
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2309 2341 2309
Size: 32 6680
PstI C_TGCA'G
Cuts at: 830 2131 3091 4064 830
Size: 1301 960 973 3478
Fragments arranged by size:
3478 1301 973 960
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6712
PvuII CAG'CTG
Cuts at: 2276 2276
Size: 6712
SacI G_AGCT'C
Cuts at: 3773 3773
Size: 6712
SacII CC_GC'GG
Cuts at: 2903 2903
Size: 6712
SalI G'TCGA_C
Cuts at: 1936 4054 1936
Size: 2118 4594
SbfI CC_TGCA'GG
Cuts at: 4064 4064
Size: 6712
ScaI AGT'ACT
Cuts at: 4960 6672 4960
Size: 1712 5000
SexAI A'CCwGG_T
Cuts at: 2637 2637
Size: 6712
SfcI C'TryA_G
Cuts at: 826 1045 2127 3087 4060 5195 5876 6067 826
Size: 219 1082 960 973 1135 681 191 1471
Fragments arranged by size:
1471 1135 1082 973 960 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6712
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6712
SmaI CCC'GGG
Cuts at: 1872 4039 1872
Size: 2167 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2567 4816 5717 5979 820
Size: 197 926 624 2249 901 262 1553
Fragments arranged by size:
2249 1553 926 901 624 262 197
SnaBI TAC'GTA
Cuts at: 493 4503 493
Size: 4010 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6712
SphI G_CATG'C
Cuts at: 1132 4070 1132
Size: 2938 3774
SspI AAT'ATT
Cuts at: 5 52 4636 5
Size: 47 4584 2081
Fragments arranged by size:
4584 2081 47
StuI AGG'CCT
Cuts at: 1517 2519 1517
Size: 1002 5710
StyI C'CwwG_G
Cuts at: 513 1065 2004 2664 3019 3924 4151 513
Size: 552 939 660 355 905 227 3074
Fragments arranged by size:
3074 939 905 660 552 355 227
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2401 2858 3575 4739 4756 4909 5094
Size: 1255 821 457 717 1164 17 153 185
Cuts at: 5094 5539 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1164 821 717 457 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2325 4005 4958
Size: 274 80 33 51 1443 348 1680 953
Cuts at: 4958 6670 96
Size: 1712 138
Fragments arranged by size:
1712 1680 1443 953 348 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3210 3240 3242 3272 6672 6704 3210
Size: 30 2 30 3400 32 3218
Fragments arranged by size:
3400 3218 32 30 30 2
Tth111I GACn'n_nGTC
Cuts at: 1316 3869 1316
Size: 2553 4159
XbaI T'CTAG_A
Cuts at: 4048 4048
Size: 6712
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3359 3359
Size: 6712
XhoI C'TCGA_G
Cuts at: 1943 2567 1943
Size: 624 6088
XmaI C'CCGG_G
Cuts at: 1870 4037 1870
Size: 2167 4545
XmnI GAAnn'nnTTC
Cuts at: 3490 4841 3490
Size: 1351 5361
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4520 276
Size: 53 83 186 936 2986 2468
Fragments arranged by size:
2986 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspXI PsrI PstI PvuI PvuII SacI SacII
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI RsrII SanDI SapI SfiI
SfoI SrfI SwaI