Restriction map for
pFN21AE6752
(Circular) (Six-base) MAPSORT of: pFN21AE6752.seq Check: 9836 from: 1 to: 6580
pFN21AE6752 6580 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2411 3268 2411
Size: 857 5723
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4390 278
Size: 53 83 186 936 2854 2468
Fragments arranged by size:
2854 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3923 1937
Size: 1986 4594
Acc65I G'GTAC_C
Cuts at: 3901 3901
Size: 6580
AclI AA'CG_TT
Cuts at: 1563 4707 5080 1563
Size: 3144 373 3063
Fragments arranged by size:
3144 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2306 2513 3371 4643 6027 6295 1632
Size: 674 207 858 1272 1384 268 1917
Fragments arranged by size:
1917 1384 1272 858 674 268 207
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6383
AflIII A'CryG_T
Cuts at: 1203 2029 2991 3114 3516 5479 6456 1203
Size: 826 962 123 402 1963 977 1327
Fragments arranged by size:
1963 1327 977 962 826 402 123
AgeI A'CCGG_T
Cuts at: 5411 5411
Size: 6580
AhdI GACnn_n'nnGTC
Cuts at: 5309 5309
Size: 6580
AleI CACnn'nnGTG
Cuts at: 1166 3257 1166
Size: 2091 4489
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2190 1184
Size: 1006 5574
AlwNI CAG_nnn'CTG
Cuts at: 2141 3016 3310 3646 4323 5895 2141
Size: 875 294 336 677 1572 2826
Fragments arranged by size:
2826 1572 875 677 336 294
ApaI G_GGCC'C
Cuts at: 2757 2757
Size: 6580
ApaLI G'TGCA_C
Cuts at: 4637 5793 4637
Size: 1156 5424
ApoI r'AATT_y
Cuts at: 1493 3889 4115 4267 6376 1493
Size: 2396 226 152 2109 1697
Fragments arranged by size:
2396 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5134 160
Size: 872 4102 1606
Fragments arranged by size:
4102 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6580
AvaI C'yCGr_G
Cuts at: 1870 1943 2756 3854 3905 1870
Size: 73 813 1098 51 4545
Fragments arranged by size:
4545 1098 813 73 51
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3207 3240 3207
Size: 33 6547
BamHI G'GATC_C
Cuts at: 3910 4277 3910
Size: 367 6213
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2327 3901 5356 618
Size: 325 217 627 540 1574 1455 1842
Fragments arranged by size:
1842 1574 1455 627 540 325 217
BanII G_rGCy'C
Cuts at: 1391 2757 2911 3138 3899 1391
Size: 1366 154 227 761 4072
Fragments arranged by size:
4072 1366 761 227 154
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2596 2670 3217 3296 928
Size: 971 697 74 547 79 4212
Fragments arranged by size:
4212 971 697 547 79 74
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4771 4805 1903
Size: 34 50 34 2750 34 3678
Fragments arranged by size:
3678 2750 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4472 5682 4472
Size: 1210 5370
BclI T'GATC_A
Cuts at: 1548 3103 1548
Size: 1555 5025
BfrBI ATG'CAT
Cuts at: 3606 3606
Size: 6580
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5191 136
Size: 107 122 71 631 605 3519 1525
Fragments arranged by size:
3519 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2181 6575 2181
Size: 4394 2186
BlpI GC'TnA_GC
Cuts at: 3132 3443 3731 3997 3132
Size: 311 288 266 5715
Fragments arranged by size:
5715 311 288 266
Bme1580I G_kGCm'C
Cuts at: 1790 2330 2757 4641 5797 1790
Size: 540 427 1884 1156 2573
Fragments arranged by size:
2573 1884 1156 540 427
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6580
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3148 4311 5269
Size: 416 53 83 399 24 1731 1163 958
Cuts at: 5269 442
Size: 1753
Fragments arranged by size:
1753 1731 1163 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6580
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2689 2996 5240 1239
Size: 1450 307 2244 2579
Fragments arranged by size:
2579 2244 1450 307
Bpu10I CC'TnA_GC
Cuts at: 3765 3765
Size: 6580
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4705 5570 5868 4705
Size: 865 298 5417
Fragments arranged by size:
5417 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2033 2768 5243 882
Size: 617 534 735 2475 2219
Fragments arranged by size:
2475 2219 735 617 534
BsaAI yAC'GTr
Cuts at: 493 1204 2992 4371 493
Size: 711 1788 1379 2702
Fragments arranged by size:
2702 1788 1379 711
BsaBI GATnn'nnATC
Cuts at: 3719 6574 3719
Size: 2855 3725
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4387 4769 5404 275
Size: 53 83 186 936 2854 382 635 1451
Fragments arranged by size:
2854 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3744 5013 5411 5685 5832 6243 3744
Size: 1269 398 274 147 411 4081
Fragments arranged by size:
4081 1269 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6355
Fragments arranged by size:
6355 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2207 2764 3608 1186
Size: 1021 557 844 4158
Fragments arranged by size:
4158 1021 844 557
BseYI C'CCAG_C
Cuts at: 2264 3639 3821 5783 6279 2264
Size: 1375 182 1962 496 2565
Fragments arranged by size:
2565 1962 1375 496 182
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3281 1163
Size: 573 1545 4462
Fragments arranged by size:
4462 1545 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4030 4791 5819 1540
Size: 275 186 2029 761 1028 2301
Fragments arranged by size:
2301 2029 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2739 2911 3138 3484 3752 3817 4641 4726 5797
Size: 172 227 346 268 65 824 85 1071
Cuts at: 5797 2739
Size: 3522
Fragments arranged by size:
3522 1071 824 346 268 227 172 85
65
BsmI GAATG_Cn'
Cuts at: 2345 4091 4184 2345
Size: 1746 93 4741
Fragments arranged by size:
4741 1746 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2330 2739 2757 2911 3138 3484 3752
Size: 399 540 409 18 154 227 346 268
Cuts at: 3752 3817 3899 4641 4726 5797 1391
Size: 65 82 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 540 409 399 346 268
227 154 85 82 65 18
BspEI T'CCGG_A
Cuts at: 6243 6243
Size: 6580
BspHI T'CATG_A
Cuts at: 4469 6199 4469
Size: 1730 4850
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2411 3268 3935 844
Size: 1567 857 667 3489
Fragments arranged by size:
3489 1567 857 667
BsrBI CCG'CTC
Cuts at: 1394 1823 2698 4467 1394
Size: 429 875 1769 3507
Fragments arranged by size:
3507 1769 875 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2174 2415 2856 3024 5075 5249 65
Size: 1183 926 241 441 168 2051 174 1396
Fragments arranged by size:
2051 1396 1183 926 441 241 174 168
BsrFI r'CCGG_y
Cuts at: 1700 1810 5224 5411 1700
Size: 110 3414 187 2869
Fragments arranged by size:
3414 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6580
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6580
BssSI C'ACGA_G
Cuts at: 4640 5652 4640
Size: 1012 5568
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2710 794
Size: 1916 4664
BstBI TT'CG_AA
Cuts at: 5384 5384
Size: 6580
BstEII G'GTnAC_C
Cuts at: 1184 2917 1184
Size: 1733 4847
BstXI CCAn_nnnn'nTGG
Cuts at: 3062 3062
Size: 6580
BstYI r'GATC_y
Cuts at: 1972 2122 2181 3501 3858 3910 4277 4662 4679
Size: 150 59 1320 357 52 367 385 17
Cuts at: 4679 6131 6217 6229 6575 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1320 385 367 357 346 150
86 59 52 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2250 3085 3324 513
Size: 552 1185 835 239 3769
Fragments arranged by size:
3769 1185 835 552 239
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3706 487
Size: 683 56 2480 3361
Fragments arranged by size:
3361 2480 683 56
BtsI GCAGTG_nn'
Cuts at: 3389 3840 4096 4890 4910 3389
Size: 451 256 794 20 5059
Fragments arranged by size:
5059 794 451 256 20
ClaI AT'CG_AT
Cuts at: 1554 3213 1554
Size: 1659 4921
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6545
DraI TTT'AAA
Cuts at: 3884 3884
Size: 6580
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5587 809
Size: 507 4271 1802
Fragments arranged by size:
4271 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2335 2643 3386
Size: 54 1425 50 275 18 505 308 743
Cuts at: 3386 3564 4027 4916 6545 8
Size: 178 463 889 1629 43
Fragments arranged by size:
1629 1425 889 743 505 463 308 275
178 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4027 1537
Size: 275 2215 4090
Fragments arranged by size:
4090 2215 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2510 2951 3554 4510 1353
Size: 1157 441 603 956 3423
Fragments arranged by size:
3423 1157 956 603 441
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5167 5541 5687 387
Size: 1064 138 3578 374 146 1280
Fragments arranged by size:
3578 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2909 3136 2909
Size: 227 6353
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2306 2513 2689 2996 3371 4643 5240
Size: 393 674 207 176 307 375 1272 597
Cuts at: 5240 6027 6295 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1272 787 674 597 393 375 307
268 207 176
EcoNI CCTnn'n_nnAGG
Cuts at: 3254 3254
Size: 6580
EcoO109I rG'GnC_Cy
Cuts at: 3149 3307 3149
Size: 158 6422
EcoRI G'AATT_C
Cuts at: 3889 4267 3889
Size: 378 6202
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3376 3376
Size: 6580
FspI TGC'GCA
Cuts at: 5086 5086
Size: 6580
HaeII r_GCGC'y
Cuts at: 1678 3563 5727 1678
Size: 1885 2164 2531
Fragments arranged by size:
2531 2164 1885
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2107 2139 2368 2400 2511
Size: 32 135 32 535 32 229 32 111
Cuts at: 2511 2543 5221 5253 5295 5327 1373
Size: 32 2678 32 42 32 2626
Fragments arranged by size:
2678 2626 535 229 135 111 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3924 4170 669
Size: 988 281 1986 246 3079
Fragments arranged by size:
3079 1986 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2727 748
Size: 1979 4601
HpaI GTT'AAC
Cuts at: 4170 4170
Size: 6580
KpnI G_GTAC'C
Cuts at: 3905 3905
Size: 6580
MfeI C'AATT_G
Cuts at: 4179 4179
Size: 6580
MluI A'CGCG_T
Cuts at: 6456 6456
Size: 6580
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2707 3501 5694 5878 1305
Size: 1402 794 2193 184 2007
Fragments arranged by size:
2193 2007 1402 794 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2645 3388 3566 10
Size: 54 1425 343 813 743 178 3024
Fragments arranged by size:
3024 1425 813 743 343 178 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2357 2540 2951 3257 4538 4897 5056
Size: 648 1191 183 411 306 1281 359 159
Cuts at: 5056 518
Size: 2042
Fragments arranged by size:
2042 1281 1191 648 411 359 306 183
159
MspA1I CmG'CkG
Cuts at: 1606 2084 2374 2600 2769 3577 3996 4673 5821
Size: 478 290 226 169 808 419 677 1148
Cuts at: 5821 6066 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 808 677 478 419 290 245
226 169
NcoI C'CATG_G
Cuts at: 513 1065 2250 513
Size: 552 1185 4843
Fragments arranged by size:
4843 1185 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6580
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6580
NotI GC'GGCC_GC
Cuts at: 4027 4027
Size: 6580
NsiI A_TGCA'T
Cuts at: 3608 3608
Size: 6580
NspI r_CATG'y
Cuts at: 1132 1229 2033 3118 3520 3938 5483 1132
Size: 97 804 1085 402 418 1545 2229
Fragments arranged by size:
2229 1545 1085 804 418 402 97
PasI CC'CwG_GG
Cuts at: 1348 3016 1348
Size: 1668 4912
PciI A'CATG_T
Cuts at: 2029 3114 3516 5479 2029
Size: 1085 402 1963 3130
Fragments arranged by size:
3130 1963 1085 402
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3310 3520 1108
Size: 2202 210 4168
Fragments arranged by size:
4168 2202 210
PfoI T'CCnGG_A
Cuts at: 2187 2777 2880 2187
Size: 590 103 5887
Fragments arranged by size:
5887 590 103
PmeI GTTT'AAAC
Cuts at: 3884 3884
Size: 6580
PmlI CAC'GTG
Cuts at: 2992 2992
Size: 6580
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4649 4681 6162 6194 4649
Size: 32 1481 32 5035
Fragments arranged by size:
5035 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3149 3149
Size: 6580
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6580
PsiI TTA'TAA
Cuts at: 4150 4150
Size: 6580
PspOMI G'GGCC_C
Cuts at: 2753 2753
Size: 6580
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3699 3731 3699
Size: 32 6548
PstI C_TGCA'G
Cuts at: 830 3576 3784 3932 830
Size: 2746 208 148 3478
Fragments arranged by size:
3478 2746 208 148
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6580
PvuII CAG'CTG
Cuts at: 2084 2374 2600 2084
Size: 290 226 6064
Fragments arranged by size:
6064 290 226
SacI G_AGCT'C
Cuts at: 2911 3138 2911
Size: 227 6353
SalI G'TCGA_C
Cuts at: 1936 3922 1936
Size: 1986 4594
SanDI GG'GwC_CC
Cuts at: 3149 3149
Size: 6580
SapI GCTCTTCn'nnn_
Cuts at: 3554 3554
Size: 6580
SbfI CC_TGCA'GG
Cuts at: 3932 3932
Size: 6580
ScaI AGT'ACT
Cuts at: 4828 6540 4828
Size: 1712 4868
SexAI A'CCwGG_T
Cuts at: 2997 2997
Size: 6580
SfcI C'TryA_G
Cuts at: 826 1045 2227 2923 3267 3572 3780 3928 5063
Size: 219 1182 696 344 305 208 148 1135
Cuts at: 5063 5744 5935 826
Size: 681 191 1471
Fragments arranged by size:
1471 1182 1135 696 681 344 305 219
208 191 148
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6580
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6580
SmaI CCC'GGG
Cuts at: 1872 3907 1872
Size: 2035 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3854 4684 5585 5847 820
Size: 197 926 1911 830 901 262 1553
Fragments arranged by size:
1911 1553 926 901 830 262 197
SnaBI TAC'GTA
Cuts at: 493 4371 493
Size: 3878 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6580
SphI G_CATG'C
Cuts at: 1132 3938 1132
Size: 2806 3774
SspI AAT'ATT
Cuts at: 5 52 4504 5
Size: 47 4452 2081
Fragments arranged by size:
4452 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6580
StyI C'CwwG_G
Cuts at: 513 1065 2250 3594 4019 513
Size: 552 1185 1344 425 3074
Fragments arranged by size:
3074 1344 1185 552 425
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3445 4607 4624 4777 4962 5407 325
Size: 1255 1865 1162 17 153 185 445 1498
Fragments arranged by size:
1865 1498 1255 1162 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3473 4826 6538
Size: 274 80 33 51 1443 1496 1353 1712
Cuts at: 6538 96
Size: 138
Fragments arranged by size:
1712 1496 1443 1353 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2773 2805 6540 6572 2773
Size: 32 3735 32 2781
Fragments arranged by size:
3735 2781 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6580
XbaI T'CTAG_A
Cuts at: 2125 3916 2125
Size: 1791 4789
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2257 2809 2257
Size: 552 6028
XhoI C'TCGA_G
Cuts at: 1943 3854 1943
Size: 1911 4669
XmaI C'CCGG_G
Cuts at: 1870 3905 1870
Size: 2035 4545
XmnI GAAnn'nnTTC
Cuts at: 2238 4709 2238
Size: 2471 4109
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4388 276
Size: 53 83 186 936 2854 2468
Fragments arranged by size:
2854 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PsrI PstI PvuI PvuII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BbvCI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PspXI RsrII SacII
SfiI SfoI SrfI SwaI