Restriction map for
pFN21AE6749
(Circular) (Six-base) MAPSORT of: pFN21AE6749.seq Check: 2327 from: 1 to: 6331
pFN21AE6749 6331 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2740 2740
Size: 6331
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4141 278
Size: 53 83 186 936 2605 2468
Fragments arranged by size:
2605 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3674 1937
Size: 1737 4594
Acc65I G'GTAC_C
Cuts at: 2263 3652 2263
Size: 1389 4942
AclI AA'CG_TT
Cuts at: 1563 4458 4831 1563
Size: 2895 373 3063
Fragments arranged by size:
3063 2895 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2040 2316 3051 4394 5778 6046 1632
Size: 408 276 735 1343 1384 268 1917
Fragments arranged by size:
1917 1384 1343 735 408 276 268
AfeI AGC'GCT
Cuts at: 3029 3029
Size: 6331
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6134
AflIII A'CryG_T
Cuts at: 1203 3372 5230 6207 1203
Size: 2169 1858 977 1327
Fragments arranged by size:
2169 1858 1327 977
AgeI A'CCGG_T
Cuts at: 2600 5162 2600
Size: 2562 3769
AhdI GACnn_n'nnGTC
Cuts at: 5060 5060
Size: 6331
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6331
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6331
AlwNI CAG_nnn'CTG
Cuts at: 2194 3061 4074 5646 2194
Size: 867 1013 1572 2879
Fragments arranged by size:
2879 1572 1013 867
ApaLI G'TGCA_C
Cuts at: 4388 5544 4388
Size: 1156 5175
ApoI r'AATT_y
Cuts at: 1493 2558 2581 3640 3866 4018 6127 1493
Size: 1065 23 1059 226 152 2109 1697
Fragments arranged by size:
2109 1697 1065 1059 226 152 23
AseI AT'TA_AT
Cuts at: 160 1032 4885 160
Size: 872 3853 1606
Fragments arranged by size:
3853 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6331
AvaI C'yCGr_G
Cuts at: 1870 1943 3008 3415 3656 1870
Size: 73 1065 407 241 4545
Fragments arranged by size:
4545 1065 407 241 73
AvrII C'CTAG_G
Cuts at: 3301 3301
Size: 6331
BamHI G'GATC_C
Cuts at: 3661 4028 3661
Size: 367 5964
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2263 2624 2741 3652 5107
Size: 325 217 627 476 361 117 911 1455
Cuts at: 5107 618
Size: 1842
Fragments arranged by size:
1842 1455 911 627 476 361 325 217
117
BanII G_rGCy'C
Cuts at: 1391 2735 3009 3422 3473 3650 1391
Size: 1344 274 413 51 177 4072
Fragments arranged by size:
4072 1344 413 274 177 51
BbeI G_GCGC'C
Cuts at: 2745 2745
Size: 6331
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2152 2755 3057 3084 3369 928
Size: 971 253 603 302 27 285 3890
Fragments arranged by size:
3890 971 603 302 285 253 27
BbvCI CC'TCA_GC
Cuts at: 3228 3394 3623 3228
Size: 166 229 5936
Fragments arranged by size:
5936 229 166
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4522 4556 1903
Size: 34 2585 34 3678
Fragments arranged by size:
3678 2585 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4223 5433 4223
Size: 1210 5121
BclI T'GATC_A
Cuts at: 1548 3252 1548
Size: 1704 4627
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4942 136
Size: 107 122 71 631 605 3270 1525
Fragments arranged by size:
3270 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6326 6326
Size: 6331
BlpI GC'TnA_GC
Cuts at: 2226 2786 3748 2226
Size: 560 962 4809
Fragments arranged by size:
4809 962 560
Bme1580I G_kGCm'C
Cuts at: 1790 4392 5548 1790
Size: 2602 1156 2573
Fragments arranged by size:
2602 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6331
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3138 3210 4062
Size: 416 53 83 399 24 1721 72 852
Cuts at: 4062 5020 442
Size: 958 1753
Fragments arranged by size:
1753 1721 958 852 416 399 83 72
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6331
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2216 3595 4991 1239
Size: 977 1379 1396 2579
Fragments arranged by size:
2579 1396 1379 977
Bpu10I CC'TnA_GC
Cuts at: 3228 3394 3623 3228
Size: 166 229 5936
Fragments arranged by size:
5936 229 166
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4456 5321 5619 4456
Size: 865 298 5168
Fragments arranged by size:
5168 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4994 882
Size: 617 3495 2219
Fragments arranged by size:
3495 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4122 493
Size: 711 2918 2702
Fragments arranged by size:
2918 2702 711
BsaBI GATnn'nnATC
Cuts at: 2954 6325 2954
Size: 3371 2960
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2334 2742 2832 4138
Size: 53 83 186 936 801 408 90 1306
Cuts at: 4138 4520 5155 275
Size: 382 635 1451
Fragments arranged by size:
1451 1306 936 801 635 408 382 186
90 83 53
BsaWI w'CCGG_w
Cuts at: 2600 2886 4764 5162 5436 5583 5994 2600
Size: 286 1878 398 274 147 411 2937
Fragments arranged by size:
2937 1878 411 398 286 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3274 3304 1178
Size: 30 165 30 1871 30 4205
Fragments arranged by size:
4205 1871 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2657 3064 3168 3592 1186
Size: 1471 407 104 424 3925
Fragments arranged by size:
3925 1471 424 407 104
BseYI C'CCAG_C
Cuts at: 3054 5534 6030 3054
Size: 2480 496 3355
Fragments arranged by size:
3355 2480 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3118 1163
Size: 573 1382 4376
Fragments arranged by size:
4376 1382 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3781 4542 5570 1540
Size: 275 186 1780 761 1028 2301
Fragments arranged by size:
2301 1780 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2735 2787 4392 4477 5548 2735
Size: 52 1605 85 1071 3518
Fragments arranged by size:
3518 1605 1071 85 52
BsmI GAATG_Cn'
Cuts at: 2550 3842 3935 2550
Size: 1292 93 4946
Fragments arranged by size:
4946 1292 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2321 2823 2321
Size: 502 5829
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2735 2787 3009 3422 3473 3650 4392
Size: 399 945 52 222 413 51 177 742
Cuts at: 4392 4477 5548 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 945 742 413 399 222 177
85 52 51
BspEI T'CCGG_A
Cuts at: 5994 5994
Size: 6331
BspHI T'CATG_A
Cuts at: 2649 3255 4220 5950 2649
Size: 606 965 1730 3030
Fragments arranged by size:
3030 1730 965 606
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2740 2749 3686 844
Size: 1896 9 937 3489
Fragments arranged by size:
3489 1896 937 9
BsrBI CCG'CTC
Cuts at: 1394 1823 3412 4218 1394
Size: 429 1589 806 3507
Fragments arranged by size:
3507 1589 806 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2378 2986 4826 5000 65
Size: 1183 1130 608 1840 174 1396
Fragments arranged by size:
1840 1396 1183 1130 608 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2600 3188 3569 4975 5162 1700
Size: 110 790 588 381 1406 187 2869
Fragments arranged by size:
2869 1406 790 588 381 187 110
BsrGI T'GTAC_A
Cuts at: 96 2095 96
Size: 1999 4332
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6331
BssSI C'ACGA_G
Cuts at: 4391 5403 4391
Size: 1012 5319
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2206 794
Size: 1412 4919
BstBI TT'CG_AA
Cuts at: 5135 5135
Size: 6331
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6331
BstYI r'GATC_y
Cuts at: 1972 3661 4028 4413 4430 5882 5968 5980 6326
Size: 1689 367 385 17 1452 86 12 346
Cuts at: 6326 1972
Size: 1977
Fragments arranged by size:
1977 1689 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5779
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5592
Fragments arranged by size:
5592 683 56
BtsI GCAGTG_nn'
Cuts at: 3847 4641 4661 3847
Size: 794 20 5517
Fragments arranged by size:
5517 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6331
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6296
DraI TTT'AAA
Cuts at: 3635 3635
Size: 6331
DraIII CAC_nnn'GTG
Cuts at: 2221 2221
Size: 6331
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2156 5338 809
Size: 507 840 3182 1802
Fragments arranged by size:
3182 1802 840 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2081 2481 2725
Size: 54 1425 50 275 18 251 400 244
Cuts at: 2725 2808 3022 3186 3384 3478 3778 4667 6296
Size: 83 214 164 198 94 300 889 1629
Cuts at: 6296 8
Size: 43
Fragments arranged by size:
1629 1425 889 400 300 275 251 244
214 198 164 94 83 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3778 1537
Size: 275 1966 4090
Fragments arranged by size:
4090 1966 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2032 3088 4261 1353
Size: 679 1056 1173 3423
Fragments arranged by size:
3423 1173 1056 679
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4918 5292 5438 387
Size: 1064 138 3329 374 146 1280
Fragments arranged by size:
3329 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2733 2733
Size: 6331
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2040 2216 2316 3051 3595 4394 4991
Size: 393 408 176 100 735 544 799 597
Cuts at: 4991 5778 6046 1239
Size: 787 268 1524
Fragments arranged by size:
1524 799 787 735 597 544 408 393
268 176 100
EcoNI CCTnn'n_nnAGG
Cuts at: 2252 2252
Size: 6331
EcoO109I rG'GnC_Cy
Cuts at: 2428 2698 2814 2428
Size: 270 116 5945
Fragments arranged by size:
5945 270 116
EcoRI G'AATT_C
Cuts at: 3640 4018 3640
Size: 378 5953
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2779 2779
Size: 6331
FspI TGC'GCA
Cuts at: 4837 4837
Size: 6331
HaeII r_GCGC'y
Cuts at: 1678 2745 3031 3173 5478 1678
Size: 1067 286 142 2305 2531
Fragments arranged by size:
2531 2305 1067 286 142
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2996 3028 3409 3441 4972
Size: 32 135 32 1424 32 381 32 1531
Cuts at: 4972 5004 5046 5078 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1531 1424 381 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2308 3675 3921 669
Size: 988 281 370 1367 246 3079
Fragments arranged by size:
3079 1367 988 370 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6331
HpaI GTT'AAC
Cuts at: 3921 3921
Size: 6331
KasI G'GCGC_C
Cuts at: 2741 2741
Size: 6331
KpnI G_GTAC'C
Cuts at: 2267 3656 2267
Size: 1389 4942
MfeI C'AATT_G
Cuts at: 3930 3930
Size: 6331
MluI A'CGCG_T
Cuts at: 6207 6207
Size: 6331
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2159 5445 5629 1305
Size: 854 3286 184 2007
Fragments arranged by size:
3286 2007 854 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2483 2810 3024 10
Size: 54 1425 343 651 327 214 3317
Fragments arranged by size:
3317 1425 651 343 327 214 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2750 4289 4648 4807 518
Size: 648 1584 1539 359 159 2042
Fragments arranged by size:
2042 1584 1539 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2191 2345 2667 2806 3747 4424 5572 5817
Size: 585 154 322 139 941 677 1148 245
Cuts at: 5817 1606
Size: 2120
Fragments arranged by size:
2120 1148 941 677 585 322 245 154
139
NaeI GCC'GGC
Cuts at: 3190 3190
Size: 6331
NarI GG'CG_CC
Cuts at: 2742 2742
Size: 6331
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5779
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6331
NgoMIV G'CCGG_C
Cuts at: 3188 3188
Size: 6331
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6331
NotI GC'GGCC_GC
Cuts at: 3778 3778
Size: 6331
NspI r_CATG'y
Cuts at: 1132 1229 2979 3376 3689 5234 1132
Size: 97 1750 397 313 1545 2229
Fragments arranged by size:
2229 1750 1545 397 313 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6331
PciI A'CATG_T
Cuts at: 3372 5230 3372
Size: 1858 4473
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6331
PmeI GTTT'AAAC
Cuts at: 3635 3635
Size: 6331
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4400 4432 5913 5945 4400
Size: 32 1481 32 4786
Fragments arranged by size:
4786 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2428 2698 2814 2428
Size: 270 116 5945
Fragments arranged by size:
5945 270 116
PshAI GACnn'nnGTC
Cuts at: 1613 2333 1613
Size: 720 5611
PsiI TTA'TAA
Cuts at: 3901 3901
Size: 6331
PstI C_TGCA'G
Cuts at: 830 2424 3683 830
Size: 1594 1259 3478
Fragments arranged by size:
3478 1594 1259
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6331
PvuII CAG'CTG
Cuts at: 2191 2191
Size: 6331
SacI G_AGCT'C
Cuts at: 2735 2735
Size: 6331
SalI G'TCGA_C
Cuts at: 1936 3673 1936
Size: 1737 4594
SbfI CC_TGCA'GG
Cuts at: 3683 3683
Size: 6331
ScaI AGT'ACT
Cuts at: 2842 4579 6291 2842
Size: 1737 1712 2882
Fragments arranged by size:
2882 1737 1712
SexAI A'CCwGG_T
Cuts at: 2151 3275 2151
Size: 1124 5207
SfcI C'TryA_G
Cuts at: 826 1045 2420 2495 3499 3679 4814 5495 5686
Size: 219 1375 75 1004 180 1135 681 191
Cuts at: 5686 826
Size: 1471
Fragments arranged by size:
1471 1375 1135 1004 681 219 191 180
75
SfoI GGC'GCC
Cuts at: 2743 2743
Size: 6331
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6331
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6331
SmaI CCC'GGG
Cuts at: 1872 3658 1872
Size: 1786 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3415 4435 5336 5598 820
Size: 197 926 1472 1020 901 262 1553
Fragments arranged by size:
1553 1472 1020 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4122 493
Size: 3629 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6331
SphI G_CATG'C
Cuts at: 1132 3689 1132
Size: 2557 3774
SspI AAT'ATT
Cuts at: 5 52 2375 4255 5
Size: 47 2323 1880 2081
Fragments arranged by size:
2323 2081 1880 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6331
StyI C'CwwG_G
Cuts at: 513 1065 2539 3301 3487 3770 513
Size: 552 1474 762 186 283 3074
Fragments arranged by size:
3074 1474 762 552 283 186
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3500 4358 4375 4528 4713 5158 325
Size: 1255 1920 858 17 153 185 445 1498
Fragments arranged by size:
1920 1498 1255 858 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2095 2117 2840
Size: 274 80 33 51 1443 118 22 723
Cuts at: 2840 4577 6289 96
Size: 1737 1712 138
Fragments arranged by size:
1737 1712 1443 723 274 138 118 80
51 33 22
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3362 3394 6291 6323 3362
Size: 32 2897 32 3370
Fragments arranged by size:
3370 2897 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3037 1316
Size: 1721 4610
XbaI T'CTAG_A
Cuts at: 3667 3667
Size: 6331
XhoI C'TCGA_G
Cuts at: 1943 3415 1943
Size: 1472 4859
XmaI C'CCGG_G
Cuts at: 1870 3656 1870
Size: 1786 4545
XmnI GAAnn'nnTTC
Cuts at: 2411 4460 2411
Size: 2049 4282
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4139 276
Size: 53 83 186 936 2605 2468
Fragments arranged by size:
2605 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI BaeI BfrBI BplI BsiWI BstXI
BstZ17I Bsu36I EcoRV FseI FspAI NruI NsiI PacI
PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI
SapI SfiI SrfI SwaI XcmI