Restriction map for
pFN21AE6746
(Circular) (Six-base) MAPSORT of: pFN21AE6746.seq Check: 5903 from: 1 to: 8269
pFN21AE6746 8269 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3256 5297 3256
Size: 2041 6228
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6079 278
Size: 53 83 186 936 4543 2468
Fragments arranged by size:
4543 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3164 5612 1937
Size: 1227 2448 4594
Fragments arranged by size:
4594 2448 1227
Acc65I G'GTAC_C
Cuts at: 5590 5590
Size: 8269
AclI AA'CG_TT
Cuts at: 1563 4732 6396 6769 1563
Size: 3169 1664 373 3063
Fragments arranged by size:
3169 3063 1664 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3372 3738 4331 6332 7716 7984 1632
Size: 1740 366 593 2001 1384 268 1917
Fragments arranged by size:
2001 1917 1740 1384 593 366 268
AflII C'TTAA_G
Cuts at: 820 1017 2036 820
Size: 197 1019 7053
Fragments arranged by size:
7053 1019 197
AflIII A'CryG_T
Cuts at: 1203 2684 7168 8145 1203
Size: 1481 4484 977 1327
Fragments arranged by size:
4484 1481 1327 977
AgeI A'CCGG_T
Cuts at: 7100 7100
Size: 8269
AhdI GACnn_n'nnGTC
Cuts at: 4977 6998 4977
Size: 2021 6248
AleI CACnn'nnGTG
Cuts at: 1166 2366 4363 4506 1166
Size: 1200 1997 143 4929
Fragments arranged by size:
4929 1997 1200 143
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4381 5471 5496 1184
Size: 3197 1090 25 3957
Fragments arranged by size:
3957 3197 1090 25
AlwNI CAG_nnn'CTG
Cuts at: 2956 5323 6012 7584 2956
Size: 2367 689 1572 3641
Fragments arranged by size:
3641 2367 1572 689
ApaI G_GGCC'C
Cuts at: 2733 3010 2733
Size: 277 7992
ApaLI G'TGCA_C
Cuts at: 6326 7482 6326
Size: 1156 7113
ApoI r'AATT_y
Cuts at: 1493 2204 2328 2896 3707 4012 4258 5078 5578
Size: 711 124 568 811 305 246 820 500
Cuts at: 5578 5804 5956 8065 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 820 811 711 568 500 305
246 226 152 124
AseI AT'TA_AT
Cuts at: 160 1032 4143 6823 160
Size: 872 3111 2680 1606
Fragments arranged by size:
3111 2680 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8269
AvaI C'yCGr_G
Cuts at: 1870 1943 2130 5337 5594 1870
Size: 73 187 3207 257 4545
Fragments arranged by size:
4545 3207 257 187 73
BamHI G'GATC_C
Cuts at: 2064 5599 5966 2064
Size: 3535 367 4367
Fragments arranged by size:
4367 3535 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2161 2368 4469 5590 7045
Size: 325 217 627 374 207 2101 1121 1455
Cuts at: 7045 618
Size: 1842
Fragments arranged by size:
2101 1842 1455 1121 627 374 325 217
207
BanII G_rGCy'C
Cuts at: 1391 2733 2999 3010 3976 5207 5588 1391
Size: 1342 266 11 966 1231 381 4072
Fragments arranged by size:
4072 1342 1231 966 381 266 11
BbeI G_GCGC'C
Cuts at: 4473 4473
Size: 8269
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3378 3619 5429 928
Size: 971 1479 241 1810 3768
Fragments arranged by size:
3768 1810 1479 971 241
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 6460 6494 1903
Size: 34 50 34 4439 34 3678
Fragments arranged by size:
4439 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3077 6161 7371 3077
Size: 3084 1210 3975
Fragments arranged by size:
3975 3084 1210
BclI T'GATC_A
Cuts at: 1548 2376 4290 5403 1548
Size: 828 1914 1113 4414
Fragments arranged by size:
4414 1914 1113 828
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4223 6880 136
Size: 107 122 71 631 605 2551 2657 1525
Fragments arranged by size:
2657 2551 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2808 8264 2808
Size: 5456 2813
BlpI GC'TnA_GC
Cuts at: 5686 5686
Size: 8269
Bme1580I G_kGCm'C
Cuts at: 1790 2733 3010 6330 7486 1790
Size: 943 277 3320 1156 2573
Fragments arranged by size:
3320 2573 1156 943 277
BmgBI CAC'GTC
Cuts at: 1318 2139 4282 1318
Size: 821 2143 5305
Fragments arranged by size:
5305 2143 821
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3196 6000 6958
Size: 416 53 83 399 24 1779 2804 958
Cuts at: 6958 442
Size: 1753
Fragments arranged by size:
2804 1779 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8269
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2177 4633 4973 5154 5327 6929 1239
Size: 938 2456 340 181 173 1602 2579
Fragments arranged by size:
2579 2456 1602 938 340 181 173
Bpu10I CC'TnA_GC
Cuts at: 4826 5201 4826
Size: 375 7894
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2900 3153 3170 5475 6394 7259 7557 2900
Size: 253 17 2305 919 865 298 3612
Fragments arranged by size:
3612 2305 919 865 298 253 17
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2142 3030 5054 6932 882
Size: 617 643 888 2024 1878 2219
Fragments arranged by size:
2219 2024 1878 888 643 617
BsaAI yAC'GTr
Cuts at: 493 1204 2687 4504 6060 493
Size: 711 1483 1817 1556 2702
Fragments arranged by size:
2702 1817 1556 1483 711
BsaBI GATnn'nnATC
Cuts at: 3474 4168 8263 3474
Size: 694 4095 3480
Fragments arranged by size:
4095 3480 694
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4470 5426 5466 6076
Size: 53 83 186 936 2937 956 40 610
Cuts at: 6076 6458 7093 275
Size: 382 635 1451
Fragments arranged by size:
2937 1451 956 936 635 610 382 186
83 53 40
BsaWI w'CCGG_w
Cuts at: 4335 6702 7100 7374 7521 7932 4335
Size: 2367 398 274 147 411 4672
Fragments arranged by size:
4672 2367 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2514 2544 4307 4337 1178
Size: 30 165 30 1111 30 1763 30 5110
Fragments arranged by size:
5110 1763 1111 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3402 3938 4354 4386 5324 1186
Size: 2216 536 416 32 938 4131
Fragments arranged by size:
4131 2216 938 536 416 32
BseYI C'CCAG_C
Cuts at: 3616 4264 4836 5413 7472 7968 3616
Size: 648 572 577 2059 496 3917
Fragments arranged by size:
3917 2059 648 577 572 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2969 3352 4228 5336 1163
Size: 573 1233 383 876 1108 4096
Fragments arranged by size:
4096 1233 1108 876 573 383
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5398 5719 6480 7508 1540
Size: 275 186 3397 321 761 1028 2301
Fragments arranged by size:
3397 2301 1028 761 321 275 186
BsiHKAI G_wGCw'C
Cuts at: 2999 3976 5207 6330 6415 7486 2999
Size: 977 1231 1123 85 1071 3782
Fragments arranged by size:
3782 1231 1123 1071 977 85
BsmI GAATG_Cn'
Cuts at: 3492 5780 5873 3492
Size: 2288 93 5888
Fragments arranged by size:
5888 2288 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2627 4458 2627
Size: 1831 6438
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2733 2999 3010 3976 5207 5588 6330
Size: 399 943 266 11 966 1231 381 742
Cuts at: 6330 6415 7486 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1231 1071 966 943 742 399 381
266 85 11
BspEI T'CCGG_A
Cuts at: 4335 7932 4335
Size: 3597 4672
BspHI T'CATG_A
Cuts at: 3315 6158 7888 3315
Size: 2843 1730 3696
Fragments arranged by size:
3696 2843 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3256 5297 5624 844
Size: 2412 2041 327 3489
Fragments arranged by size:
3489 2412 2041 327
BsrBI CCG'CTC
Cuts at: 1394 1823 2070 6156 1394
Size: 429 247 4086 3507
Fragments arranged by size:
4086 3507 429 247
BsrDI GCAATG_nn'
Cuts at: 65 1248 2310 2604 3336 5163 5300 5377 6764
Size: 1183 1062 294 732 1827 137 77 1387
Cuts at: 6764 6938 65
Size: 174 1396
Fragments arranged by size:
1827 1396 1387 1183 1062 732 294 174
137 77
BsrFI r'CCGG_y
Cuts at: 1700 1810 6913 7100 1700
Size: 110 5103 187 2869
Fragments arranged by size:
5103 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4749 96
Size: 4653 3616
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8269
BssSI C'ACGA_G
Cuts at: 4363 6329 7341 4363
Size: 1966 1012 5291
Fragments arranged by size:
5291 1966 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2444 794
Size: 1650 6619
BstBI TT'CG_AA
Cuts at: 3745 4329 7073 3745
Size: 584 2744 4941
Fragments arranged by size:
4941 2744 584
BstEII G'GTnAC_C
Cuts at: 1184 4627 1184
Size: 3443 4826
BstYI r'GATC_y
Cuts at: 1972 2064 2808 2952 5599 5966 6351 6368 7820
Size: 92 744 144 2647 367 385 17 1452
Cuts at: 7820 7906 7918 8264 1972
Size: 86 12 346 1977
Fragments arranged by size:
2647 1977 1452 744 385 367 346 144
92 86 17 12
BstZ17I GTA'TAC
Cuts at: 3165 3165
Size: 8269
Bsu36I CC'TnA_GG
Cuts at: 2592 2592
Size: 8269
BtgI C'CryG_G
Cuts at: 513 1065 2051 2076 3228 3864 513
Size: 552 986 25 1152 636 4918
Fragments arranged by size:
4918 1152 986 636 552 25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3680 4060 4549 487
Size: 683 56 2454 380 489 4207
Fragments arranged by size:
4207 2454 683 489 380 56
BtsI GCAGTG_nn'
Cuts at: 2523 2667 4240 4894 5785 6579 6599 2523
Size: 144 1573 654 891 794 20 4193
Fragments arranged by size:
4193 1573 891 794 654 144 20
ClaI AT'CG_AT
Cuts at: 1554 4296 1554
Size: 2742 5527
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2125 2160 529
Size: 35 1561 35 6638
Fragments arranged by size:
6638 1561 35 35
DraI TTT'AAA
Cuts at: 5573 5573
Size: 8269
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7276 809
Size: 507 5960 1802
Fragments arranged by size:
5960 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2229 3231 3516
Size: 54 1425 50 275 18 399 1002 285
Cuts at: 3516 4300 5716 6605 8234 8
Size: 784 1416 889 1629 43
Fragments arranged by size:
1629 1425 1416 1002 889 784 399 285
275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5716 1537
Size: 275 3904 4090
Fragments arranged by size:
4090 3904 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2278 2312 3249 3580 3981 4934 6199 1353
Size: 925 34 937 331 401 953 1265 3423
Fragments arranged by size:
3423 1265 953 937 925 401 331 34
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6856 7230 7376 387
Size: 1064 138 5267 374 146 1280
Fragments arranged by size:
5267 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2997 3974 5205 2997
Size: 977 1231 6061
Fragments arranged by size:
6061 1231 977
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2177 3372 3738 4331 4633 4973 5154
Size: 393 545 1195 366 593 302 340 181
Cuts at: 5154 5327 6332 6929 7716 7984 1239
Size: 173 1005 597 787 268 1524
Fragments arranged by size:
1524 1195 1005 787 597 593 545 393
366 340 302 268 181 173
EcoNI CCTnn'n_nnAGG
Cuts at: 4196 5459 4196
Size: 1263 7006
EcoO109I rG'GnC_Cy
Cuts at: 2081 2729 3006 3007 2081
Size: 648 277 1 7343
Fragments arranged by size:
7343 648 277 1
EcoRI G'AATT_C
Cuts at: 2896 5578 5956 2896
Size: 2682 378 5209
Fragments arranged by size:
5209 2682 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3637 4067 4657 3637
Size: 430 590 7249
Fragments arranged by size:
7249 590 430
FspI TGC'GCA
Cuts at: 6775 6775
Size: 8269
HaeII r_GCGC'y
Cuts at: 1678 2912 4473 7416 1678
Size: 1234 1561 2943 2531
Fragments arranged by size:
2943 2531 1561 1234
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2111 2143 3053 3085 3397
Size: 32 135 32 539 32 910 32 312
Cuts at: 3397 3429 3543 3575 4963 4995 6910 6942 6984
Size: 32 114 32 1388 32 1915 32 42
Cuts at: 6984 7016 1373
Size: 32 2626
Fragments arranged by size:
2626 1915 1388 910 539 312 135 114
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2247 2452 4116 4971 5613 5859
Size: 988 281 309 205 1664 855 642 246
Cuts at: 5859 669
Size: 3079
Fragments arranged by size:
3079 1664 988 855 642 309 281 246
205
HindIII A'AGCT_T
Cuts at: 748 4589 748
Size: 3841 4428
HpaI GTT'AAC
Cuts at: 2452 5859 2452
Size: 3407 4862
KasI G'GCGC_C
Cuts at: 4469 4469
Size: 8269
KpnI G_GTAC'C
Cuts at: 5594 5594
Size: 8269
MfeI C'AATT_G
Cuts at: 2391 5868 2391
Size: 3477 4792
MluI A'CGCG_T
Cuts at: 8145 8145
Size: 8269
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2125 2369 3383 3963 4984 7383 7567 1305
Size: 820 244 1014 580 1021 2399 184 2007
Fragments arranged by size:
2399 2007 1021 1014 820 580 244 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3233 4302 10
Size: 54 1425 343 1401 1069 3977
Fragments arranged by size:
3977 1425 1401 1069 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2366 2745 3197 4363 4506 5478 6227
Size: 648 1200 379 452 1166 143 972 749
Cuts at: 6227 6586 6745 518
Size: 359 159 2042
Fragments arranged by size:
2042 1200 1166 972 749 648 452 379
359 159 143
MspA1I CmG'CkG
Cuts at: 1606 2053 2155 5685 6362 7510 7755 1606
Size: 447 102 3530 677 1148 245 2120
Fragments arranged by size:
3530 2120 1148 677 447 245 102
NarI GG'CG_CC
Cuts at: 4470 4470
Size: 8269
NcoI C'CATG_G
Cuts at: 513 1065 3228 513
Size: 552 2163 5554
Fragments arranged by size:
5554 2163 552
NdeI CA'TA_TG
Cuts at: 387 4379 5023 387
Size: 3992 644 3633
Fragments arranged by size:
3992 3633 644
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8269
NotI GC'GGCC_GC
Cuts at: 5716 5716
Size: 8269
NspI r_CATG'y
Cuts at: 1132 1229 2680 3309 4537 4825 5110 5627 7172
Size: 97 1451 629 1228 288 285 517 1545
Cuts at: 7172 1132
Size: 2229
Fragments arranged by size:
2229 1545 1451 1228 629 517 288 285
97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8269
PciI A'CATG_T
Cuts at: 7168 7168
Size: 8269
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2502 5070 1108
Size: 1394 2568 4307
Fragments arranged by size:
4307 2568 1394
PfoI T'CCnGG_A
Cuts at: 3702 4347 5558 3702
Size: 645 1211 6413
Fragments arranged by size:
6413 1211 645
PmeI GTTT'AAAC
Cuts at: 5573 5573
Size: 8269
PmlI CAC'GTG
Cuts at: 2687 4504 2687
Size: 1817 6452
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4673 4705 6338 6370 7851 7883 4673
Size: 32 1633 32 1481 32 5059
Fragments arranged by size:
5059 1633 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2081 2081
Size: 8269
PshAI GACnn'nnGTC
Cuts at: 1613 3399 1613
Size: 1786 6483
PsiI TTA'TAA
Cuts at: 5839 5839
Size: 8269
PspOMI G'GGCC_C
Cuts at: 2729 3006 2729
Size: 277 7992
PstI C_TGCA'G
Cuts at: 830 4388 4404 5460 5621 830
Size: 3558 16 1056 161 3478
Fragments arranged by size:
3558 3478 1056 161 16
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8269
PvuII CAG'CTG
Cuts at: 2155 2155
Size: 8269
SacI G_AGCT'C
Cuts at: 2999 3976 5207 2999
Size: 977 1231 6061
Fragments arranged by size:
6061 1231 977
SacII CC_GC'GG
Cuts at: 2054 2054
Size: 8269
SalI G'TCGA_C
Cuts at: 1936 5611 1936
Size: 3675 4594
SapI GCTCTTCn'nnn_
Cuts at: 2278 3249 2278
Size: 971 7298
SbfI CC_TGCA'GG
Cuts at: 5621 5621
Size: 8269
ScaI AGT'ACT
Cuts at: 6517 8229 6517
Size: 1712 6557
SfcI C'TryA_G
Cuts at: 826 1045 2715 3418 4384 4400 5456 5617 6752
Size: 219 1670 703 966 16 1056 161 1135
Cuts at: 6752 7433 7624 826
Size: 681 191 1471
Fragments arranged by size:
1670 1471 1135 1056 966 703 681 219
191 161 16
SfoI GGC'GCC
Cuts at: 4471 4471
Size: 8269
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8269
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8269
SmaI CCC'GGG
Cuts at: 1872 2132 5596 1872
Size: 260 3464 4545
Fragments arranged by size:
4545 3464 260
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2036 2915 3149 3168 5337 5490
Size: 197 926 93 879 234 19 2169 153
Cuts at: 5490 6373 7274 7536 820
Size: 883 901 262 1553
Fragments arranged by size:
2169 1553 926 901 883 879 262 234
197 153 93 19
SnaBI TAC'GTA
Cuts at: 493 6060 493
Size: 5567 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8269
SphI G_CATG'C
Cuts at: 1132 4537 4825 5110 5627 1132
Size: 3405 288 285 517 3774
Fragments arranged by size:
3774 3405 517 288 285
SspI AAT'ATT
Cuts at: 5 52 5011 6193 5
Size: 47 4959 1182 2081
Fragments arranged by size:
4959 2081 1182 47
StuI AGG'CCT
Cuts at: 1517 2022 3572 1517
Size: 505 1550 6214
Fragments arranged by size:
6214 1550 505
StyI C'CwwG_G
Cuts at: 513 1065 2794 2988 3228 4252 4908 5708 513
Size: 552 1729 194 240 1024 656 800 3074
Fragments arranged by size:
3074 1729 1024 800 656 552 240 194
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4099 4496 4868 6296 6313 6466 6651
Size: 1255 2519 397 372 1428 17 153 185
Cuts at: 6651 7096 325
Size: 445 1498
Fragments arranged by size:
2519 1498 1428 1255 445 397 372 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2235 3012 4644
Size: 274 80 33 51 1443 258 777 1632
Cuts at: 4644 4749 6515 8227 96
Size: 105 1766 1712 138
Fragments arranged by size:
1766 1712 1632 1443 777 274 258 138
105 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2514 2546 3520 3552 3871 3903 5340 5372 8229
Size: 32 974 32 319 32 1437 32 2857
Cuts at: 8229 8261 2514
Size: 32 2522
Fragments arranged by size:
2857 2522 1437 974 319 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8269
XbaI T'CTAG_A
Cuts at: 5605 5605
Size: 8269
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3136 4948 3136
Size: 1812 6457
XhoI C'TCGA_G
Cuts at: 1943 5337 1943
Size: 3394 4875
XmaI C'CCGG_G
Cuts at: 1870 2130 5594 1870
Size: 260 3464 4545
Fragments arranged by size:
4545 3464 260
XmnI GAAnn'nnTTC
Cuts at: 3751 6398 3751
Size: 2647 5622
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6077 276
Size: 53 83 186 936 4543 2468
Fragments arranged by size:
4543 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BstXI DraIII EcoRV FseI FspAI NaeI NgoMIV
NruI NsiI PacI PspXI PsrI RsrII SanDI SexAI
SfiI SrfI SwaI