Restriction map for
pFN21AE6740
(Circular) (Six-base) MAPSORT of: pFN21AE6740.seq Check: 9084 from: 1 to: 7033
pFN21AE6740 7033 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3091 4109 3091
Size: 1018 6015
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3669 4843 278
Size: 53 83 186 936 2133 1174 2468
Fragments arranged by size:
2468 2133 1174 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3417 4376 1937
Size: 1480 959 4594
Fragments arranged by size:
4594 1480 959
Acc65I G'GTAC_C
Cuts at: 4354 4354
Size: 7033
AclI AA'CG_TT
Cuts at: 1563 4332 5160 5533 1563
Size: 2769 828 373 3063
Fragments arranged by size:
3063 2769 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2085 4224 5096 6480 6748 1632
Size: 453 2139 872 1384 268 1917
Fragments arranged by size:
2139 1917 1384 872 453 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6836
AflIII A'CryG_T
Cuts at: 1203 3636 3714 5932 6909 1203
Size: 2433 78 2218 977 1327
Fragments arranged by size:
2433 2218 1327 977 78
AgeI A'CCGG_T
Cuts at: 5864 5864
Size: 7033
AhdI GACnn_n'nnGTC
Cuts at: 5762 5762
Size: 7033
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7033
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7033
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2867 2899 2867
Size: 32 7001
AlwNI CAG_nnn'CTG
Cuts at: 2287 2572 4776 6348 2287
Size: 285 2204 1572 2972
Fragments arranged by size:
2972 2204 1572 285
ApaI G_GGCC'C
Cuts at: 2327 2327
Size: 7033
ApaLI G'TGCA_C
Cuts at: 5090 6246 5090
Size: 1156 5877
ApoI r'AATT_y
Cuts at: 1493 3980 4342 4568 4720 6829 1493
Size: 2487 362 226 152 2109 1697
Fragments arranged by size:
2487 2109 1697 362 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5587 160
Size: 872 4555 1606
Fragments arranged by size:
4555 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7033
AvaI C'yCGr_G
Cuts at: 1870 1943 3306 4358 1870
Size: 73 1363 1052 4545
Fragments arranged by size:
4545 1363 1052 73
BamHI G'GATC_C
Cuts at: 4363 4730 4363
Size: 367 6666
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3448 4354 5809 618
Size: 325 217 627 1661 906 1455 1842
Fragments arranged by size:
1842 1661 1455 906 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2327 3656 4281 4352 1391
Size: 936 1329 625 71 4072
Fragments arranged by size:
4072 1329 936 625 71
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2655 928
Size: 971 756 5306
Fragments arranged by size:
5306 971 756
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5224 5258 1903
Size: 34 3287 34 3678
Fragments arranged by size:
3678 3287 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2584 4925 6135 2584
Size: 2341 1210 3482
Fragments arranged by size:
3482 2341 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7033
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2342 5644 136
Size: 107 122 71 631 605 670 3302 1525
Fragments arranged by size:
3302 1525 670 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7028 7028
Size: 7033
BlpI GC'TnA_GC
Cuts at: 4094 4280 4450 4094
Size: 186 170 6677
Fragments arranged by size:
6677 186 170
Bme1580I G_kGCm'C
Cuts at: 1790 2327 3453 5094 6250 1790
Size: 537 1126 1641 1156 2573
Fragments arranged by size:
2573 1641 1156 1126 537
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7033
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2275 2560 4764
Size: 416 53 83 399 24 858 285 2204
Cuts at: 4764 5722 442
Size: 958 1753
Fragments arranged by size:
2204 1753 958 858 416 399 285 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7033
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2394 2765 2804 3335 3530 5693 1239
Size: 1155 371 39 531 195 2163 2579
Fragments arranged by size:
2579 2163 1155 531 371 195 39
Bpu10I CC'TnA_GC
Cuts at: 3764 3764
Size: 7033
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3851 4065 5158 6023 6321 3851
Size: 214 1093 865 298 4563
Fragments arranged by size:
4563 1093 865 298 214
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2234 2780 4256 5696 882
Size: 617 735 546 1476 1440 2219
Fragments arranged by size:
2219 1476 1440 735 617 546
BsaAI yAC'GTr
Cuts at: 493 1204 4824 493
Size: 711 3620 2702
Fragments arranged by size:
3620 2702 711
BsaBI GATnn'nnATC
Cuts at: 7027 7027
Size: 7033
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3666 4840 5222 5857
Size: 53 83 186 936 2133 1174 382 635
Cuts at: 5857 275
Size: 1451
Fragments arranged by size:
2133 1451 1174 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2602 3877 4242 5466 5864 6138 6285 6696 2602
Size: 1275 365 1224 398 274 147 411 2939
Fragments arranged by size:
2939 1275 1224 411 398 365 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2087 2117 2584 2614 3311
Size: 30 165 30 684 30 467 30 697
Cuts at: 3311 3341 1178
Size: 30 4870
Fragments arranged by size:
4870 697 684 467 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2622 3285 3332 3389 3854 3918 1186
Size: 1436 663 47 57 465 64 4301
Fragments arranged by size:
4301 1436 663 465 64 57 47
BseYI C'CCAG_C
Cuts at: 6236 6732 6236
Size: 496 6537
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2580 3101 1163
Size: 573 844 521 5095
Fragments arranged by size:
5095 844 573 521
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2994 3364 3785 4483 5244 6272
Size: 275 186 993 370 421 698 761 1028
Cuts at: 6272 1540
Size: 2301
Fragments arranged by size:
2301 1028 993 761 698 421 370 275
186
BsiHKAI G_wGCw'C
Cuts at: 2118 2751 3738 3770 4100 4281 5094 5179 6250
Size: 633 987 32 330 181 813 85 1071
Cuts at: 6250 2118
Size: 2901
Fragments arranged by size:
2901 1071 987 813 633 330 181 85
32
BsmI GAATG_Cn'
Cuts at: 4250 4544 4637 4250
Size: 294 93 6646
Fragments arranged by size:
6646 294 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2118 2327 2751 3453 3656 3738 3770
Size: 399 328 209 424 702 203 82 32
Cuts at: 3770 4100 4281 4352 5094 5179 6250 1391
Size: 330 181 71 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 702 424 399 330 328
209 203 181 85 82 71 32
BspEI T'CCGG_A
Cuts at: 2602 4242 6696 2602
Size: 1640 2454 2939
Fragments arranged by size:
2939 2454 1640
BspHI T'CATG_A
Cuts at: 2893 4922 6652 2893
Size: 2029 1730 3274
Fragments arranged by size:
3274 2029 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3091 4109 4388 844
Size: 2247 1018 279 3489
Fragments arranged by size:
3489 2247 1018 279
BsrBI CCG'CTC
Cuts at: 1394 1823 4143 4920 1394
Size: 429 2320 777 3507
Fragments arranged by size:
3507 2320 777 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3915 4071 4279 5528 5702 65
Size: 1183 2667 156 208 1249 174 1396
Fragments arranged by size:
2667 1396 1249 1183 208 174 156
BsrFI r'CCGG_y
Cuts at: 1700 1810 2856 3168 3800 5677 5864 1700
Size: 110 1046 312 632 1877 187 2869
Fragments arranged by size:
2869 1877 1046 632 312 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7033
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7033
BssSI C'ACGA_G
Cuts at: 5093 6105 5093
Size: 1012 6021
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2405 3019 794
Size: 1611 614 4808
Fragments arranged by size:
4808 1611 614
BstBI TT'CG_AA
Cuts at: 4219 5837 4219
Size: 1618 5415
BstEII G'GTnAC_C
Cuts at: 1184 2428 1184
Size: 1244 5789
BstXI CCAn_nnnn'nTGG
Cuts at: 2494 3730 2494
Size: 1236 5797
BstYI r'GATC_y
Cuts at: 1972 3909 4239 4363 4730 5115 5132 6584 6670
Size: 1937 330 124 367 385 17 1452 86
Cuts at: 6670 6682 7028 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1937 1452 385 367 346 330 124
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3372 3372
Size: 7033
BtgI C'CryG_G
Cuts at: 513 1065 2004 2036 2552 2582 3344 3853 513
Size: 552 939 32 516 30 762 509 3693
Fragments arranged by size:
3693 939 762 552 516 509 32 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6294
Fragments arranged by size:
6294 683 56
BtsI GCAGTG_nn'
Cuts at: 2636 3834 4549 5343 5363 2636
Size: 1198 715 794 20 4306
Fragments arranged by size:
4306 1198 794 715 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7033
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2346 2381 529
Size: 35 1782 35 5181
Fragments arranged by size:
5181 1782 35 35
DraI TTT'AAA
Cuts at: 4337 4337
Size: 7033
DraIII CAC_nnn'GTG
Cuts at: 3820 3820
Size: 7033
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6040 809
Size: 507 4724 1802
Fragments arranged by size:
4724 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2343 2496 2991
Size: 54 1425 50 275 18 513 153 495
Cuts at: 2991 3361 3782 4480 5369 6998 8
Size: 370 421 698 889 1629 43
Fragments arranged by size:
1629 1425 889 698 513 495 421 370
275 153 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2991 3361 3782 4480 1537
Size: 275 1179 370 421 698 4090
Fragments arranged by size:
4090 1179 698 421 370 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2118 2601 2927 3581 3866 4305 4963 1353
Size: 765 483 326 654 285 439 658 3423
Fragments arranged by size:
3423 765 658 654 483 439 326 285
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2108 2603 5620 5994 6140 387
Size: 1064 138 519 495 3017 374 146 1280
Fragments arranged by size:
3017 1280 1064 519 495 374 146 138
EcoICRI GAG'CTC
Cuts at: 4279 4279
Size: 7033
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2085 2394 2765 2804 3335 3530 4224
Size: 393 453 309 371 39 531 195 694
Cuts at: 4224 5096 5693 6480 6748 1239
Size: 872 597 787 268 1524
Fragments arranged by size:
1524 872 787 694 597 531 453 393
371 309 268 195 39
EcoNI CCTnn'n_nnAGG
Cuts at: 3098 3533 3989 3098
Size: 435 456 6142
Fragments arranged by size:
6142 456 435
EcoO109I rG'GnC_Cy
Cuts at: 2323 2324 2503 2611 4199 2323
Size: 1 179 108 1588 5157
Fragments arranged by size:
5157 1588 179 108 1
EcoRI G'AATT_C
Cuts at: 3980 4342 4720 3980
Size: 362 378 6293
Fragments arranged by size:
6293 378 362
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2630 2630
Size: 7033
FspI TGC'GCA
Cuts at: 5539 5539
Size: 7033
HaeII r_GCGC'y
Cuts at: 1678 6180 1678
Size: 4502 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2129 2161 2216 2248 3602
Size: 32 135 32 557 32 55 32 1354
Cuts at: 3602 3634 5674 5706 5748 5780 1373
Size: 32 2040 32 42 32 2626
Fragments arranged by size:
2626 2040 1354 557 135 55 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4377 4623 669
Size: 988 281 2439 246 3079
Fragments arranged by size:
3079 2439 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7033
HpaI GTT'AAC
Cuts at: 4623 4623
Size: 7033
KpnI G_GTAC'C
Cuts at: 4358 4358
Size: 7033
MfeI C'AATT_G
Cuts at: 4632 4632
Size: 7033
MluI A'CGCG_T
Cuts at: 6909 6909
Size: 7033
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3669 6147 6331 1305
Size: 2364 2478 184 2007
Fragments arranged by size:
2478 2364 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2498 10
Size: 54 1425 343 666 4545
Fragments arranged by size:
4545 1425 666 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2985 3446 3731 3920 4991 5350 5509
Size: 648 1819 461 285 189 1071 359 159
Cuts at: 5509 518
Size: 2042
Fragments arranged by size:
2042 1819 1071 648 461 359 285 189
159
MspA1I CmG'CkG
Cuts at: 1606 2027 2038 2425 2439 2554 2584 4449 5126
Size: 421 11 387 14 115 30 1865 677
Cuts at: 5126 6274 6519 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1865 1148 677 421 387 245 115
30 14 11
NaeI GCC'GGC
Cuts at: 3170 3802 3170
Size: 632 6401
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5542
Fragments arranged by size:
5542 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7033
NgoMIV G'CCGG_C
Cuts at: 3168 3800 3168
Size: 632 6401
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7033
NotI GC'GGCC_GC
Cuts at: 2991 4480 2991
Size: 1489 5544
NspI r_CATG'y
Cuts at: 1132 1229 2704 3433 3640 3718 4004 4391 5936
Size: 97 1475 729 207 78 286 387 1545
Cuts at: 5936 1132
Size: 2229
Fragments arranged by size:
2229 1545 1475 729 387 286 207 97
78
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7033
PciI A'CATG_T
Cuts at: 3636 3714 5932 3636
Size: 78 2218 4737
Fragments arranged by size:
4737 2218 78
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2572 2815 3497 1108
Size: 1464 243 682 4644
Fragments arranged by size:
4644 1464 682 243
PfoI T'CCnGG_A
Cuts at: 2514 2514
Size: 7033
PmeI GTTT'AAAC
Cuts at: 4337 4337
Size: 7033
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5102 5134 6615 6647 5102
Size: 32 1481 32 5488
Fragments arranged by size:
5488 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4199 4199
Size: 7033
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7033
PsiI TTA'TAA
Cuts at: 4191 4603 4191
Size: 412 6621
PspOMI G'GGCC_C
Cuts at: 2323 2323
Size: 7033
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3677 3709 3677
Size: 32 7001
PstI C_TGCA'G
Cuts at: 830 2211 4140 4385 830
Size: 1381 1929 245 3478
Fragments arranged by size:
3478 1929 1381 245
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7033
SacI G_AGCT'C
Cuts at: 4281 4281
Size: 7033
SacII CC_GC'GG
Cuts at: 2039 2555 2585 2039
Size: 516 30 6487
Fragments arranged by size:
6487 516 30
SalI G'TCGA_C
Cuts at: 1936 4375 1936
Size: 2439 4594
SbfI CC_TGCA'GG
Cuts at: 4385 4385
Size: 7033
ScaI AGT'ACT
Cuts at: 3278 3335 5281 6993 3278
Size: 57 1946 1712 3318
Fragments arranged by size:
3318 1946 1712 57
SexAI A'CCwGG_T
Cuts at: 3816 3816
Size: 7033
SfcI C'TryA_G
Cuts at: 826 1045 2207 3694 4136 4381 5516 6197 6388
Size: 219 1162 1487 442 245 1135 681 191
Cuts at: 6388 826
Size: 1471
Fragments arranged by size:
1487 1471 1162 1135 681 442 245 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7033
SgrAI Cr'CCGG_yG
Cuts at: 1700 2856 1700
Size: 1156 5877
SmaI CCC'GGG
Cuts at: 1872 3308 4360 1872
Size: 1436 1052 4545
Fragments arranged by size:
4545 1436 1052
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3866 4080 5137 6038 6300 820
Size: 197 926 1923 214 1057 901 262 1553
Fragments arranged by size:
1923 1553 1057 926 901 262 214 197
SnaBI TAC'GTA
Cuts at: 493 4824 493
Size: 4331 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7033
SphI G_CATG'C
Cuts at: 1132 4391 1132
Size: 3259 3774
SspI AAT'ATT
Cuts at: 5 52 3959 4957 5
Size: 47 3907 998 2081
Fragments arranged by size:
3907 2081 998 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7033
StyI C'CwwG_G
Cuts at: 513 1065 2004 2193 2499 4472 513
Size: 552 939 189 306 1973 3074
Fragments arranged by size:
3074 1973 939 552 306 189
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2265 3827 5060 5077 5230 5415 5860
Size: 1255 685 1562 1233 17 153 185 445
Cuts at: 5860 325
Size: 1498
Fragments arranged by size:
1562 1498 1255 1233 685 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3276 3333 3708
Size: 274 80 33 51 1443 1299 57 375
Cuts at: 3708 5279 6991 96
Size: 1571 1712 138
Fragments arranged by size:
1712 1571 1443 1299 375 274 138 80
57 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3141 3173 3626 3658 6993 7025 3141
Size: 32 453 32 3335 32 3149
Fragments arranged by size:
3335 3149 453 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7033
XbaI T'CTAG_A
Cuts at: 4369 4369
Size: 7033
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2313 2362 3814 2313
Size: 49 1452 5532
Fragments arranged by size:
5532 1452 49
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7033
XmaI C'CCGG_G
Cuts at: 1870 3306 4358 1870
Size: 1436 1052 4545
Fragments arranged by size:
4545 1436 1052
XmnI GAAnn'nnTTC
Cuts at: 2261 3518 5162 2261
Size: 1257 1644 4132
Fragments arranged by size:
4132 1644 1257
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3667 4841 276
Size: 53 83 186 936 2133 1174 2468
Fragments arranged by size:
2468 2133 1174 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PsrI PstI PvuI SacI SacII SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
BsiWI BsmBI BstZ17I EcoRV FseI FspAI KasI NarI
NruI NsiI PacI PmlI PspXI PvuII RsrII SanDI
SapI SfiI SfoI SrfI SwaI