Restriction map for
pFN21AE6739
(Circular) (Six-base) MAPSORT of: pFN21AE6739.seq Check: 8309 from: 1 to: 8359
pFN21AE6739 8359 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2748 3176 4169 2748
Size: 428 993 6938
Fragments arranged by size:
6938 993 428
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6169 278
Size: 53 83 186 936 4633 2468
Fragments arranged by size:
4633 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5541 5702 1937
Size: 3604 161 4594
Fragments arranged by size:
4594 3604 161
Acc65I G'GTAC_C
Cuts at: 4413 5680 4413
Size: 1267 7092
AclI AA'CG_TT
Cuts at: 1563 6486 6859 1563
Size: 4923 373 3063
Fragments arranged by size:
4923 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2534 3789 5007 5079 5210 5487 6422 7806
Size: 902 1255 1218 72 131 277 935 1384
Cuts at: 7806 8074 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1255 1218 935 902 277 268
131 72
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8162
AflIII A'CryG_T
Cuts at: 1203 4046 4277 5041 7258 8235 1203
Size: 2843 231 764 2217 977 1327
Fragments arranged by size:
2843 2217 1327 977 764 231
AgeI A'CCGG_T
Cuts at: 4416 4530 7190 4416
Size: 114 2660 5585
Fragments arranged by size:
5585 2660 114
AhdI GACnn_n'nnGTC
Cuts at: 7088 7088
Size: 8359
AleI CACnn'nnGTG
Cuts at: 1166 4116 4280 4394 4557 4799 1166
Size: 2950 164 114 163 242 4726
Fragments arranged by size:
4726 2950 242 164 163 114
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2384 4575 1184
Size: 1200 2191 4968
Fragments arranged by size:
4968 2191 1200
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5417 5449 5417
Size: 32 8327
AlwNI CAG_nnn'CTG
Cuts at: 2512 2954 3178 3538 4245 4660 5324 6102 7674
Size: 442 224 360 707 415 664 778 1572
Cuts at: 7674 2512
Size: 3197
Fragments arranged by size:
3197 1572 778 707 664 442 415 360
224
ApaI G_GGCC'C
Cuts at: 2277 2684 4335 4449 2277
Size: 407 1651 114 6187
Fragments arranged by size:
6187 1651 407 114
ApaLI G'TGCA_C
Cuts at: 2200 4846 6416 7572 2200
Size: 2646 1570 1156 2987
Fragments arranged by size:
2987 2646 1570 1156
ApoI r'AATT_y
Cuts at: 1493 3839 4957 5668 5894 6046 8155 1493
Size: 2346 1118 711 226 152 2109 1697
Fragments arranged by size:
2346 2109 1697 1118 711 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3357 3894 4008 6913 160
Size: 872 2325 537 114 2905 1606
Fragments arranged by size:
2905 2325 1606 872 537 114
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8359
AvaI C'yCGr_G
Cuts at: 1870 1943 2098 2185 2476 2809 3659 4432 5684
Size: 73 155 87 291 333 850 773 1252
Cuts at: 5684 1870
Size: 4545
Fragments arranged by size:
4545 1252 850 773 333 291 155 87
73
BamHI G'GATC_C
Cuts at: 5689 6056 5689
Size: 367 7992
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2037 2174 2760 3164 3614
Size: 325 217 627 250 137 586 404 450
Cuts at: 3614 4157 4388 4413 4559 5277 5680 7135 618
Size: 543 231 25 146 718 403 1455 1842
Fragments arranged by size:
1842 1455 718 627 586 543 450 404
403 325 250 231 217 146 137 25
BanII G_rGCy'C
Cuts at: 1391 2277 2684 2841 4335 4449 5228 5678 1391
Size: 886 407 157 1494 114 779 450 4072
Fragments arranged by size:
4072 1494 886 779 450 407 157 114
BbeI G_GCGC'C
Cuts at: 2041 2178 2041
Size: 137 8222
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3311 4473 4809 4844 928
Size: 971 1412 1162 336 35 4443
Fragments arranged by size:
4443 1412 1162 971 336 35
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2344 2378 2697 2731 3458
Size: 34 50 34 323 34 319 34 727
Cuts at: 3458 3492 3919 3953 6550 6584 1903
Size: 34 427 34 2597 34 3678
Fragments arranged by size:
3678 2597 727 427 323 319 50 34
34 34 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6251 7461 6251
Size: 1210 7149
BclI T'GATC_A
Cuts at: 1548 2484 1548
Size: 936 7423
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2712 2769 6970
Size: 107 122 71 631 605 1040 57 4201
Cuts at: 6970 136
Size: 1525
Fragments arranged by size:
4201 1525 1040 631 605 122 107 71
57
BglII A'GATC_T
Cuts at: 2890 8354 2890
Size: 5464 2895
BlpI GC'TnA_GC
Cuts at: 3012 5222 5776 3012
Size: 2210 554 5595
Fragments arranged by size:
5595 2210 554
Bme1580I G_kGCm'C
Cuts at: 1790 2074 2204 2277 2684 3167 3206 3619 3659
Size: 284 130 73 407 483 39 413 40
Cuts at: 3659 4046 4277 4335 4432 4449 4554 4728 4739
Size: 387 231 58 97 17 105 174 11
Cuts at: 4739 4850 5629 6420 7576 1790
Size: 111 779 791 1156 2573
Fragments arranged by size:
2573 1156 791 779 483 413 407 387
284 231 174 130 111 105 97 73
58 40 39 17 11
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8359
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2875 3228 3613
Size: 416 53 83 399 24 1458 353 385
Cuts at: 3613 6090 7048 442
Size: 2477 958 1753
Fragments arranged by size:
2477 1753 1458 958 416 399 385 353
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8359
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5202 5202
Size: 8359
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4530 4811 7019 1239
Size: 3291 281 2208 2579
Fragments arranged by size:
3291 2579 2208 281
Bpu10I CC'TnA_GC
Cuts at: 2045 2290 2724 2045
Size: 245 434 7680
Fragments arranged by size:
7680 434 245
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2225 2315 6484 7349 7647 2225
Size: 90 4169 865 298 2937
Fragments arranged by size:
4169 2937 865 298 90
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2184 2852 3056 4579 7022 882
Size: 617 685 668 204 1523 2443 2219
Fragments arranged by size:
2443 2219 1523 685 668 617 204
BsaAI yAC'GTr
Cuts at: 493 1204 4047 4118 4143 4305 6150 493
Size: 711 2843 71 25 162 1845 2702
Fragments arranged by size:
2843 2702 1845 711 162 71 25
BsaBI GATnn'nnATC
Cuts at: 8353 8353
Size: 8359
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2038 2175 3387 6166
Size: 53 83 186 936 505 137 1212 2779
Cuts at: 6166 6548 7183 275
Size: 382 635 1451
Fragments arranged by size:
2779 1451 1212 936 635 505 382 186
137 83 53
BsaWI w'CCGG_w
Cuts at: 4416 4530 6792 7190 7464 7611 8022 4416
Size: 114 2262 398 274 147 411 4753
Fragments arranged by size:
4753 2262 411 398 274 147 114
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2108 2138 2237 2267 4898
Size: 30 165 30 705 30 99 30 2631
Cuts at: 4898 4928 5417 5447 1178
Size: 30 489 30 4090
Fragments arranged by size:
4090 2631 705 489 165 99 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 8359
BseYI C'CCAG_C
Cuts at: 2492 3978 4206 4323 4442 4739 7562 8058 2492
Size: 1486 228 117 119 297 2823 496 2793
Fragments arranged by size:
2823 2793 1486 496 297 228 119 117
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3721 4777 1163
Size: 573 1985 1056 4745
Fragments arranged by size:
4745 1985 1056 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2029 2268 2353 5809 6570 7598
Size: 275 186 28 239 85 3456 761 1028
Cuts at: 7598 1540
Size: 2301
Fragments arranged by size:
3456 2301 1028 761 275 239 186 85
28
BsiHKAI G_wGCw'C
Cuts at: 2204 3018 4345 4850 4938 5228 6420 6505 7576
Size: 814 1327 505 88 290 1192 85 1071
Cuts at: 7576 2204
Size: 2987
Fragments arranged by size:
2987 1327 1192 1071 814 505 290 88
85
BsmI GAATG_Cn'
Cuts at: 4478 5870 5963 4478
Size: 1392 93 6874
Fragments arranged by size:
6874 1392 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2074 2204 2277 2684 2841 3018 3167
Size: 399 284 130 73 407 157 177 149
Cuts at: 3167 3206 3619 3659 4046 4277 4335 4345 4432
Size: 39 413 40 387 231 58 10 87
Cuts at: 4432 4449 4554 4728 4739 4850 4938 5228 5629
Size: 17 105 174 11 111 88 290 401
Cuts at: 5629 5678 6420 6505 7576 1391
Size: 49 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 413 407 401 399 387
290 284 231 177 174 157 149 130
111 105 88 87 85 73 58 49
40 39 17 11 10
BspEI T'CCGG_A
Cuts at: 8022 8022
Size: 8359
BspHI T'CATG_A
Cuts at: 6248 7978 6248
Size: 1730 6629
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2748 3176 4115 4169 4658 5714 844
Size: 1904 428 939 54 489 1056 3489
Fragments arranged by size:
3489 1904 1056 939 489 428 54
BsrBI CCG'CTC
Cuts at: 1394 1823 6246 1394
Size: 429 4423 3507
Fragments arranged by size:
4423 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3294 6854 7028 65
Size: 1183 2046 3560 174 1396
Fragments arranged by size:
3560 2046 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2028 2496 3104 4416 4530 4831 5524
Size: 110 218 468 608 1312 114 301 693
Cuts at: 5524 7003 7190 1700
Size: 1479 187 2869
Fragments arranged by size:
2869 1479 1312 693 608 468 301 218
187 114 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8359
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8359
BssSI C'ACGA_G
Cuts at: 6419 7431 6419
Size: 1012 7347
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3681 794
Size: 2887 5472
BstBI TT'CG_AA
Cuts at: 7163 7163
Size: 8359
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8359
BstYI r'GATC_y
Cuts at: 1972 2118 2550 2890 5247 5689 6056 6441 6458
Size: 146 432 340 2357 442 367 385 17
Cuts at: 6458 7910 7996 8008 8354 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
2357 1977 1452 442 432 385 367 346
340 146 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2979 2979
Size: 8359
BtgI C'CryG_G
Cuts at: 513 1065 2015 2341 2821 3157 3747 4599 513
Size: 552 950 326 480 336 590 852 4273
Fragments arranged by size:
4273 950 852 590 552 480 336 326
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4987 487
Size: 683 56 3761 3859
Fragments arranged by size:
3859 3761 683 56
BtsI GCAGTG_nn'
Cuts at: 2507 2852 3700 4951 5875 6669 6689 2507
Size: 345 848 1251 924 794 20 4177
Fragments arranged by size:
4177 1251 924 848 794 345 20
ClaI AT'CG_AT
Cuts at: 1554 3468 3582 3939 5112 1554
Size: 1914 114 357 1173 4801
Fragments arranged by size:
4801 1914 1173 357 114
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2071 2106 2884 2919 3781 3816 4135
Size: 35 1507 35 778 35 862 35 319
Cuts at: 4135 4170 529
Size: 35 4718
Fragments arranged by size:
4718 1507 862 778 319 35 35 35
35 35
DraI TTT'AAA
Cuts at: 5663 5663
Size: 8359
DraIII CAC_nnn'GTG
Cuts at: 4490 4490
Size: 8359
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7366 809
Size: 507 6050 1802
Fragments arranged by size:
6050 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2026 2265 2387
Size: 54 1425 50 275 18 196 239 122
Cuts at: 2387 2872 3370 3744 5046 5806 6695 8324 8
Size: 485 498 374 1302 760 889 1629 43
Fragments arranged by size:
1629 1425 1302 889 760 498 485 374
275 239 196 122 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2026 2265 5806 1537
Size: 275 214 239 3541 4090
Fragments arranged by size:
4090 3541 275 239 214
EarI CTCTTCn'nnn_
Cuts at: 1353 5469 5544 6289 1353
Size: 4116 75 745 3423
Fragments arranged by size:
4116 3423 745 75
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5308 6946 7320 7466 387
Size: 1064 138 3719 1638 374 146 1280
Fragments arranged by size:
3719 1638 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 5226 5226
Size: 8359
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2534 3789 4530 4811 5007 5079 5210
Size: 393 902 1255 741 281 196 72 131
Cuts at: 5210 5487 6422 7019 7806 8074 1239
Size: 277 935 597 787 268 1524
Fragments arranged by size:
1524 1255 935 902 787 741 597 393
281 277 268 196 131 72
EcoNI CCTnn'n_nnAGG
Cuts at: 2389 2389
Size: 8359
EcoO109I rG'GnC_Cy
Cuts at: 2094 2273 2274 2394 2681 2951 2993 3175 3987
Size: 179 1 120 287 270 42 182 812
Cuts at: 3987 4331 2094
Size: 344 6122
Fragments arranged by size:
6122 812 344 287 270 182 179 120
42 1
EcoRI G'AATT_C
Cuts at: 5668 6046 5668
Size: 378 7981
FspI TGC'GCA
Cuts at: 6865 6865
Size: 8359
HaeII r_GCGC'y
Cuts at: 1678 2041 2178 7506 1678
Size: 363 137 5328 2531
Fragments arranged by size:
5328 2531 363 137
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2108 2140 2237 2269 4578
Size: 32 135 32 536 32 97 32 2309
Cuts at: 4578 4610 4799 4831 4898 4930 7000 7032 7074
Size: 32 189 32 67 32 2070 32 42
Cuts at: 7074 7106 1373
Size: 32 2626
Fragments arranged by size:
2626 2309 2070 536 189 135 97 67
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3184 3949 4288 5703 5949 669
Size: 988 281 1246 765 339 1415 246 3079
Fragments arranged by size:
3079 1415 1246 988 765 339 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8359
HpaI GTT'AAC
Cuts at: 3184 5949 3184
Size: 2765 5594
KasI G'GCGC_C
Cuts at: 2037 2174 2037
Size: 137 8222
KpnI G_GTAC'C
Cuts at: 4417 5684 4417
Size: 1267 7092
MfeI C'AATT_G
Cuts at: 5958 5958
Size: 8359
MluI A'CGCG_T
Cuts at: 8235 8235
Size: 8359
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2094 3780 3816 7473 7657 1305
Size: 789 1686 36 3657 184 2007
Fragments arranged by size:
3657 2007 1686 789 184 36
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3372 3746 5048 10
Size: 54 1425 343 1540 374 1302 3321
Fragments arranged by size:
3321 1540 1425 1302 374 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4116 4280 4394 4557 4598 4799 6317
Size: 648 2950 164 114 163 41 201 1518
Cuts at: 6317 6676 6835 518
Size: 359 159 2042
Fragments arranged by size:
2950 2042 1518 648 359 201 164 163
159 114 41
MspA1I CmG'CkG
Cuts at: 1606 2343 3054 3574 3735 4386 4743 4985 5359
Size: 737 711 520 161 651 357 242 374
Cuts at: 5359 5775 6452 7600 7845 1606
Size: 416 677 1148 245 2120
Fragments arranged by size:
2120 1148 737 711 677 651 520 416
374 357 245 242 161
NaeI GCC'GGC
Cuts at: 2498 5526 2498
Size: 3028 5331
NarI GG'CG_CC
Cuts at: 2038 2175 2038
Size: 137 8222
NcoI C'CATG_G
Cuts at: 513 1065 3747 4599 513
Size: 552 2682 852 4273
Fragments arranged by size:
4273 2682 852 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8359
NgoMIV G'CCGG_C
Cuts at: 2496 5524 2496
Size: 3028 5331
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8359
NotI GC'GGCC_GC
Cuts at: 5806 5806
Size: 8359
NruI TCG'CGA
Cuts at: 4053 4053
Size: 8359
NspI r_CATG'y
Cuts at: 1132 1229 3117 4195 4281 5045 5717 7262 1132
Size: 97 1888 1078 86 764 672 1545 2229
Fragments arranged by size:
2229 1888 1545 1078 764 672 97 86
PasI CC'CwG_GG
Cuts at: 1348 3070 1348
Size: 1722 6637
PciI A'CATG_T
Cuts at: 4277 5041 7258 4277
Size: 764 2217 5378
Fragments arranged by size:
5378 2217 764
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3178 1108
Size: 2070 6289
PfoI T'CCnGG_A
Cuts at: 4764 4764
Size: 8359
PmeI GTTT'AAAC
Cuts at: 5663 5663
Size: 8359
PmlI CAC'GTG
Cuts at: 4047 4118 4143 4047
Size: 71 25 8263
Fragments arranged by size:
8263 71 25
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5417 5449 6428 6460 7941 7973 5417
Size: 32 979 32 1481 32 5803
Fragments arranged by size:
5803 1481 979 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2394 2993 3175 2394
Size: 599 182 7578
Fragments arranged by size:
7578 599 182
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8359
PsiI TTA'TAA
Cuts at: 5929 5929
Size: 8359
PspOMI G'GGCC_C
Cuts at: 2273 2680 4331 4445 2273
Size: 407 1651 114 6187
Fragments arranged by size:
6187 1651 407 114
PstI C_TGCA'G
Cuts at: 830 2154 2282 2720 2759 4019 4364 4457 5711
Size: 1324 128 438 39 1260 345 93 1254
Cuts at: 5711 830
Size: 3478
Fragments arranged by size:
3478 1324 1260 1254 438 345 128 93
39
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8359
PvuII CAG'CTG
Cuts at: 3054 3574 3735 5359 3054
Size: 520 161 1624 6054
Fragments arranged by size:
6054 1624 520 161
RsrII CG'GwC_CG
Cuts at: 2031 2031
Size: 8359
SacI G_AGCT'C
Cuts at: 5228 5228
Size: 8359
SacII CC_GC'GG
Cuts at: 2344 2344
Size: 8359
SalI G'TCGA_C
Cuts at: 1936 5701 1936
Size: 3765 4594
SbfI CC_TGCA'GG
Cuts at: 5711 5711
Size: 8359
ScaI AGT'ACT
Cuts at: 6607 8319 6607
Size: 1712 6647
SexAI A'CCwGG_T
Cuts at: 3177 4056 4918 3177
Size: 879 862 6618
Fragments arranged by size:
6618 879 862
SfcI C'TryA_G
Cuts at: 826 1045 2150 2278 2716 2755 3967 4015 4360
Size: 219 1105 128 438 39 1212 48 345
Cuts at: 4360 4453 5707 6842 7523 7714 826
Size: 93 1254 1135 681 191 1471
Fragments arranged by size:
1471 1254 1212 1135 1105 681 438 345
219 191 128 93 48 39
SfoI GGC'GCC
Cuts at: 2039 2176 2039
Size: 137 8222
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8359
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8359
SmaI CCC'GGG
Cuts at: 1872 2187 2811 4434 5686 1872
Size: 315 624 1623 1252 4545
Fragments arranged by size:
4545 1623 1252 624 315
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2240 2330 6463 7364 7626 820
Size: 197 926 297 90 4133 901 262 1553
Fragments arranged by size:
4133 1553 926 901 297 262 197 90
SnaBI TAC'GTA
Cuts at: 493 6150 493
Size: 5657 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8359
SphI G_CATG'C
Cuts at: 1132 5717 1132
Size: 4585 3774
SspI AAT'ATT
Cuts at: 5 52 3355 3892 4006 4966 4991 6283 5
Size: 47 3303 537 114 960 25 1292 2081
Fragments arranged by size:
3303 2081 1292 960 537 114 47 25
StuI AGG'CCT
Cuts at: 1517 2781 1517
Size: 1264 7095
StyI C'CwwG_G
Cuts at: 513 1065 2082 3367 3747 4599 5798 513
Size: 552 1017 1285 380 852 1199 3074
Fragments arranged by size:
3074 1285 1199 1017 852 552 380
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4080 4803 5598 6386 6403 6556 6741
Size: 1255 2500 723 795 788 17 153 185
Cuts at: 6741 7186 325
Size: 445 1498
Fragments arranged by size:
2500 1498 1255 795 788 723 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2784 5649 6605
Size: 274 80 33 51 1443 807 2865 956
Cuts at: 6605 8317 96
Size: 1712 138
Fragments arranged by size:
2865 1712 1443 956 807 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4760 4792 8319 8351 4760
Size: 32 3527 32 4768
Fragments arranged by size:
4768 3527 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4058 1316
Size: 2742 5617
XbaI T'CTAG_A
Cuts at: 5695 5695
Size: 8359
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4661 4793 4661
Size: 132 8227
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8359
XmaI C'CCGG_G
Cuts at: 1870 2185 2809 4432 5684 1870
Size: 315 624 1623 1252 4545
Fragments arranged by size:
4545 1623 1252 624 315
XmnI GAAnn'nnTTC
Cuts at: 6488 6488
Size: 8359
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6167 276
Size: 53 83 186 936 4633 2468
Fragments arranged by size:
4633 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI
BstXI BstZ17I EcoRV FalI FseI FspAI NsiI PacI
PspXI PsrI SanDI SapI SfiI SrfI SwaI