Restriction map for
pFN21AE6737
(Circular) (Six-base) MAPSORT of: pFN21AE6737.seq Check: 6545 from: 1 to: 7303
pFN21AE6737 7303 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5113 278
Size: 53 83 186 936 3577 2468
Fragments arranged by size:
3577 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4646 1937
Size: 2709 4594
Acc65I G'GTAC_C
Cuts at: 4624 4624
Size: 7303
AclI AA'CG_TT
Cuts at: 1563 5430 5803 1563
Size: 3867 373 3063
Fragments arranged by size:
3867 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2862 3911 3963 4159 4559 5366 6750 7018
Size: 1230 1049 52 196 400 807 1384 268
Cuts at: 7018 1632
Size: 1917
Fragments arranged by size:
1917 1384 1230 1049 807 400 268 196
52
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7106
AflIII A'CryG_T
Cuts at: 1203 3711 6202 7179 1203
Size: 2508 2491 977 1327
Fragments arranged by size:
2508 2491 1327 977
AgeI A'CCGG_T
Cuts at: 6134 6134
Size: 7303
AhdI GACnn_n'nnGTC
Cuts at: 6032 6032
Size: 7303
AleI CACnn'nnGTG
Cuts at: 1166 3216 1166
Size: 2050 5253
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7303
AlwNI CAG_nnn'CTG
Cuts at: 2029 3243 3934 4182 5046 6618 2029
Size: 1214 691 248 864 1572 2714
Fragments arranged by size:
2714 1572 1214 864 691 248
ApaI G_GGCC'C
Cuts at: 3156 3156
Size: 7303
ApaLI G'TGCA_C
Cuts at: 5360 6516 5360
Size: 1156 6147
ApoI r'AATT_y
Cuts at: 1493 2441 2508 4612 4838 4990 7099 1493
Size: 948 67 2104 226 152 2109 1697
Fragments arranged by size:
2109 2104 1697 948 226 152 67
AseI AT'TA_AT
Cuts at: 160 1032 5857 160
Size: 872 4825 1606
Fragments arranged by size:
4825 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7303
AvaI C'yCGr_G
Cuts at: 1870 1943 2072 4628 1870
Size: 73 129 2556 4545
Fragments arranged by size:
4545 2556 129 73
BamHI G'GATC_C
Cuts at: 3246 3286 4633 5000 3246
Size: 40 1347 367 5549
Fragments arranged by size:
5549 1347 367 40
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2076 2633 4624 6079 618
Size: 325 217 627 289 557 1991 1455 1842
Fragments arranged by size:
1991 1842 1455 627 557 325 289 217
BanII G_rGCy'C
Cuts at: 1391 2069 2256 2761 3156 3426 3563 4622 1391
Size: 678 187 505 395 270 137 1059 4072
Fragments arranged by size:
4072 1059 678 505 395 270 187 137
BbeI G_GCGC'C
Cuts at: 2080 2080
Size: 7303
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4321 928
Size: 971 2422 3910
Fragments arranged by size:
3910 2422 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5494 5528 1903
Size: 34 50 34 3473 34 3678
Fragments arranged by size:
3678 3473 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5195 6405 5195
Size: 1210 6093
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7303
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5914 136
Size: 107 122 71 631 605 4242 1525
Fragments arranged by size:
4242 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3946 7298 3946
Size: 3352 3951
BlpI GC'TnA_GC
Cuts at: 3741 4720 3741
Size: 979 6324
Bme1580I G_kGCm'C
Cuts at: 1790 2638 3156 5364 6520 1790
Size: 848 518 2208 1156 2573
Fragments arranged by size:
2573 2208 1156 848 518
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7303
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3187 3313 5034
Size: 416 53 83 399 24 1770 126 1721
Cuts at: 5034 5992 442
Size: 958 1753
Fragments arranged by size:
1770 1753 1721 958 416 399 126 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7303
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3423 3423
Size: 7303
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2261 2589 2979 3458 4184 5963 1239
Size: 1022 328 390 479 726 1779 2579
Fragments arranged by size:
2579 1779 1022 726 479 390 328
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5428 6293 6591 5428
Size: 865 298 6140
Fragments arranged by size:
6140 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5966 882
Size: 617 4467 2219
Fragments arranged by size:
4467 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3123 5094 493
Size: 711 1919 1971 2702
Fragments arranged by size:
2702 1971 1919 711
BsaBI GATnn'nnATC
Cuts at: 3030 3291 4209 4350 7297 3030
Size: 261 918 141 2947 3036
Fragments arranged by size:
3036 2947 918 261 141
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2077 5110 5492 6127
Size: 53 83 186 936 544 3033 382 635
Cuts at: 6127 275
Size: 1451
Fragments arranged by size:
3033 1451 936 635 544 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2803 5736 6134 6408 6555 6966 2803
Size: 2933 398 274 147 411 3140
Fragments arranged by size:
3140 2933 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3112 3142 3431 3461 1178
Size: 30 165 30 1709 30 289 30 5020
Fragments arranged by size:
5020 1709 289 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4130 1186
Size: 2944 4359
BseYI C'CCAG_C
Cuts at: 2615 3395 3471 3902 4042 4519 6506 7002 2615
Size: 780 76 431 140 477 1987 496 2916
Fragments arranged by size:
2916 1987 780 496 477 431 140 76
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2637 1163
Size: 573 901 5829
Fragments arranged by size:
5829 901 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2098 4753 5514 6542 1540
Size: 275 186 97 2655 761 1028 2301
Fragments arranged by size:
2655 2301 1028 761 275 186 97
BsiHKAI G_wGCw'C
Cuts at: 2256 2877 5364 5449 6520 2256
Size: 621 2487 85 1071 3039
Fragments arranged by size:
3039 2487 1071 621 85
BsmI GAATG_Cn'
Cuts at: 2746 4108 4156 4814 4907 2746
Size: 1362 48 658 93 5142
Fragments arranged by size:
5142 1362 658 93 48
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2069 2256 2638 2761 2877 3156 3426
Size: 399 279 187 382 123 116 279 270
Cuts at: 3426 3563 4622 5364 5449 6520 1391
Size: 137 1059 742 85 1071 2174
Fragments arranged by size:
2174 1071 1059 742 399 382 279 279
270 187 137 123 116 85
BspEI T'CCGG_A
Cuts at: 6966 6966
Size: 7303
BspHI T'CATG_A
Cuts at: 3808 4098 5192 6922 3808
Size: 290 1094 1730 4189
Fragments arranged by size:
4189 1730 1094 290
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2273 4658 844
Size: 1429 2385 3489
Fragments arranged by size:
3489 2385 1429
BsrBI CCG'CTC
Cuts at: 1394 1823 5190 1394
Size: 429 3367 3507
Fragments arranged by size:
3507 3367 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5798 5972 65
Size: 1183 4550 174 1396
Fragments arranged by size:
4550 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4569 5947 6134 1700
Size: 110 2759 1378 187 2869
Fragments arranged by size:
2869 2759 1378 187 110
BsrGI T'GTAC_A
Cuts at: 96 4462 96
Size: 4366 2937
BssHII G'CGCG_C
Cuts at: 1925 2019 1925
Size: 94 7209
BssSI C'ACGA_G
Cuts at: 2760 5363 6375 2760
Size: 2603 1012 3688
Fragments arranged by size:
3688 2603 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2029 2612 794
Size: 1235 583 5485
Fragments arranged by size:
5485 1235 583
BstBI TT'CG_AA
Cuts at: 6107 6107
Size: 7303
BstEII G'GTnAC_C
Cuts at: 1184 2977 1184
Size: 1793 5510
BstXI CCAn_nnnn'nTGG
Cuts at: 3067 3218 3767 3067
Size: 151 549 6603
Fragments arranged by size:
6603 549 151
BstYI r'GATC_y
Cuts at: 1972 2264 3246 3286 3946 4633 5000 5385 5402
Size: 292 982 40 660 687 367 385 17
Cuts at: 5402 6854 6940 6952 7298 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 982 687 660 385 367 346
292 86 40 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3060 3453 513
Size: 552 1995 393 4363
Fragments arranged by size:
4363 1995 552 393
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6564
Fragments arranged by size:
6564 683 56
BtsI GCAGTG_nn'
Cuts at: 4011 4819 5613 5633 4011
Size: 808 794 20 5681
Fragments arranged by size:
5681 808 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7303
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7268
DraI TTT'AAA
Cuts at: 2446 2643 4607 2446
Size: 197 1964 5142
Fragments arranged by size:
5142 1964 197
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2975 6310 809
Size: 507 1659 3335 1802
Fragments arranged by size:
3335 1802 1659 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2095 2208 4750
Size: 54 1425 50 275 18 265 113 2542
Cuts at: 4750 5639 7268 8
Size: 889 1629 43
Fragments arranged by size:
2542 1629 1425 889 275 265 113 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2095 4750 1537
Size: 275 283 2655 4090
Fragments arranged by size:
4090 2655 283 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4060 4430 5233 1353
Size: 2707 370 803 3423
Fragments arranged by size:
3423 2707 803 370
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5890 6264 6410 387
Size: 1064 138 4301 374 146 1280
Fragments arranged by size:
4301 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2254 2254
Size: 7303
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2261 2589 2862 2979 3458 3911 3963
Size: 393 629 328 273 117 479 453 52
Cuts at: 3963 4159 4184 4559 5366 5963 6750 7018 1239
Size: 196 25 375 807 597 787 268 1524
Fragments arranged by size:
1524 807 787 629 597 479 453 393
375 328 273 268 196 117 52 25
EcoO109I rG'GnC_Cy
Cuts at: 2035 3152 3153 3922 2035
Size: 1117 1 769 5416
Fragments arranged by size:
5416 1117 769 1
EcoRI G'AATT_C
Cuts at: 4612 4990 4612
Size: 378 6925
EcoRV GAT'ATC
Cuts at: 4026 4026
Size: 7303
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3031 3031
Size: 7303
FspI TGC'GCA
Cuts at: 5809 5809
Size: 7303
HaeII r_GCGC'y
Cuts at: 1678 2080 4085 6450 1678
Size: 402 2005 2365 2531
Fragments arranged by size:
2531 2365 2005 402
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3739 3771 5944 5976 6018
Size: 32 135 32 2167 32 2173 32 42
Cuts at: 6018 6050 1373
Size: 32 2626
Fragments arranged by size:
2626 2173 2167 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3717 4647 4893 669
Size: 988 281 1779 930 246 3079
Fragments arranged by size:
3079 1779 988 930 281 246
HindIII A'AGCT_T
Cuts at: 748 2850 748
Size: 2102 5201
HpaI GTT'AAC
Cuts at: 3717 4893 3717
Size: 1176 6127
KasI G'GCGC_C
Cuts at: 2076 2076
Size: 7303
KpnI G_GTAC'C
Cuts at: 4628 4628
Size: 7303
MfeI C'AATT_G
Cuts at: 4902 4902
Size: 7303
MluI A'CGCG_T
Cuts at: 7179 7179
Size: 7303
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2929 4023 6417 6601 1305
Size: 1624 1094 2394 184 2007
Fragments arranged by size:
2394 2007 1624 1094 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2210 10
Size: 54 1425 343 378 5103
Fragments arranged by size:
5103 1425 378 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2817 3065 3216 5261 5620 5779 518
Size: 648 1651 248 151 2045 359 159 2042
Fragments arranged by size:
2045 2042 1651 648 359 248 159 151
MspA1I CmG'CkG
Cuts at: 1606 2026 2059 4719 5396 6544 6789 1606
Size: 420 33 2660 677 1148 245 2120
Fragments arranged by size:
2660 2120 1148 677 420 245 33
NarI GG'CG_CC
Cuts at: 2077 2077
Size: 7303
NcoI C'CATG_G
Cuts at: 513 1065 3060 3453 513
Size: 552 1995 393 4363
Fragments arranged by size:
4363 1995 552 393
NdeI CA'TA_TG
Cuts at: 387 2938 3349 4453 387
Size: 2551 411 1104 3237
Fragments arranged by size:
3237 2551 1104 411
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7303
NotI GC'GGCC_GC
Cuts at: 4750 4750
Size: 7303
NspI r_CATG'y
Cuts at: 1132 1229 2477 3715 4661 6206 1132
Size: 97 1248 1238 946 1545 2229
Fragments arranged by size:
2229 1545 1248 1238 946 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7303
PciI A'CATG_T
Cuts at: 3711 6202 3711
Size: 2491 4812
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3002 1108
Size: 1894 5409
PfoI T'CCnGG_A
Cuts at: 2305 2566 2305
Size: 261 7042
PmeI GTTT'AAAC
Cuts at: 4607 4607
Size: 7303
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2475 2507 2781 2813 5372 5404 6885 6917 2475
Size: 32 274 32 2559 32 1481 32 2861
Fragments arranged by size:
2861 2559 1481 274 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3922 3922
Size: 7303
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7303
PsiI TTA'TAA
Cuts at: 4873 4873
Size: 7303
PspOMI G'GGCC_C
Cuts at: 3152 3152
Size: 7303
PstI C_TGCA'G
Cuts at: 830 2064 2131 2749 4655 830
Size: 1234 67 618 1906 3478
Fragments arranged by size:
3478 1906 1234 618 67
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7303
PvuII CAG'CTG
Cuts at: 2026 2026
Size: 7303
SacI G_AGCT'C
Cuts at: 2256 2256
Size: 7303
SalI G'TCGA_C
Cuts at: 1936 4645 1936
Size: 2709 4594
SbfI CC_TGCA'GG
Cuts at: 4655 4655
Size: 7303
ScaI AGT'ACT
Cuts at: 5551 7263 5551
Size: 1712 5591
SfcI C'TryA_G
Cuts at: 826 1045 2060 2127 2745 3186 4417 4423 4651
Size: 219 1015 67 618 441 1231 6 228
Cuts at: 4651 5786 6467 6658 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1231 1135 1015 681 618 441 228
219 191 67 6
SfoI GGC'GCC
Cuts at: 2078 2078
Size: 7303
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7303
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7303
SmaI CCC'GGG
Cuts at: 1872 2074 4630 1872
Size: 202 2556 4545
Fragments arranged by size:
4545 2556 202
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5407 6308 6570 820
Size: 197 926 3464 901 262 1553
Fragments arranged by size:
3464 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5094 493
Size: 4601 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7303
SphI G_CATG'C
Cuts at: 1132 2477 4661 1132
Size: 1345 2184 3774
Fragments arranged by size:
3774 2184 1345
SspI AAT'ATT
Cuts at: 5 52 3364 4235 5227 5
Size: 47 3312 871 992 2081
Fragments arranged by size:
3312 2081 992 871 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7303
StyI C'CwwG_G
Cuts at: 513 1065 3060 3453 3723 4299 4332 4742 513
Size: 552 1995 393 270 576 33 410 3074
Fragments arranged by size:
3074 1995 576 552 410 393 270 33
SwaI ATTT'AAAT
Cuts at: 2446 2446
Size: 7303
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3169 3736 4568 5330 5347 5500 5685
Size: 1255 1589 567 832 762 17 153 185
Cuts at: 5685 6130 325
Size: 445 1498
Fragments arranged by size:
1589 1498 1255 832 762 567 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3321 3516 4462
Size: 274 80 33 51 1443 1344 195 946
Cuts at: 4462 5549 7261 96
Size: 1087 1712 138
Fragments arranged by size:
1712 1443 1344 1087 946 274 195 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2617 2649 3112 3144 3431 3463 4473 4505 7263
Size: 32 463 32 287 32 1010 32 2758
Cuts at: 7263 7295 2617
Size: 32 2625
Fragments arranged by size:
2758 2625 1010 463 287 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7303
XbaI T'CTAG_A
Cuts at: 3028 4639 3028
Size: 1611 5692
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3652 3652
Size: 7303
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7303
XmaI C'CCGG_G
Cuts at: 1870 2072 4628 1870
Size: 202 2556 4545
Fragments arranged by size:
4545 2556 202
XmnI GAAnn'nnTTC
Cuts at: 2508 3069 4172 5432 2508
Size: 561 1103 1260 4379
Fragments arranged by size:
4379 1260 1103 561
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5111 276
Size: 53 83 186 936 3577 2468
Fragments arranged by size:
3577 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI
PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI