Restriction map for
pFN21AE6736
(Circular) (Six-base) MAPSORT of: pFN21AE6736.seq Check: 8799 from: 1 to: 9430
pFN21AE6736 9430 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2802 3212 3837 4823 4890 5214 6322 2802
Size: 410 625 986 67 324 1108 5910
Fragments arranged by size:
5910 1108 986 625 410 324 67
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5190 7240 278
Size: 53 83 186 936 3654 2050 2468
Fragments arranged by size:
3654 2468 2050 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5190 6773 1937
Size: 3253 1583 4594
Fragments arranged by size:
4594 3253 1583
Acc65I G'GTAC_C
Cuts at: 5634 6751 5634
Size: 1117 8313
AclI AA'CG_TT
Cuts at: 1563 7557 7930 1563
Size: 5994 373 3063
Fragments arranged by size:
5994 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2429 5105 5715 5820 5834 6366 7493 8877
Size: 797 2676 610 105 14 532 1127 1384
Cuts at: 8877 9145 1632
Size: 268 1917
Fragments arranged by size:
2676 1917 1384 1127 797 610 532 268
105 14
AfeI AGC'GCT
Cuts at: 2105 2231 2912 3101 2105
Size: 126 681 189 8434
Fragments arranged by size:
8434 681 189 126
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9233
AflIII A'CryG_T
Cuts at: 1203 2160 2730 8329 9306 1203
Size: 957 570 5599 977 1327
Fragments arranged by size:
5599 1327 977 957 570
AgeI A'CCGG_T
Cuts at: 8261 8261
Size: 9430
AhdI GACnn_n'nnGTC
Cuts at: 3160 4483 8159 3160
Size: 1323 3676 4431
Fragments arranged by size:
4431 3676 1323
AleI CACnn'nnGTG
Cuts at: 1166 2399 3069 4952 6276 1166
Size: 1233 670 1883 1324 4320
Fragments arranged by size:
4320 1883 1324 1233 670
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4606 4711 5418 6294 1184
Size: 3422 105 707 876 4320
Fragments arranged by size:
4320 3422 876 707 105
AlwNI CAG_nnn'CTG
Cuts at: 2539 3291 3441 4704 5142 5293 5531 5996 6337
Size: 752 150 1263 438 151 238 465 341
Cuts at: 6337 6364 6517 6625 6682 7173 8745 2539
Size: 27 153 108 57 491 1572 3224
Fragments arranged by size:
3224 1572 1263 752 491 465 438 341
238 153 151 150 108 57 27
ApaI G_GGCC'C
Cuts at: 2947 4196 5545 2947
Size: 1249 1349 6832
Fragments arranged by size:
6832 1349 1249
ApaLI G'TGCA_C
Cuts at: 4810 7487 8643 4810
Size: 2677 1156 5597
Fragments arranged by size:
5597 2677 1156
ApoI r'AATT_y
Cuts at: 1493 3112 6739 6965 7117 9226 1493
Size: 1619 3627 226 152 2109 1697
Fragments arranged by size:
3627 2109 1697 1619 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7984 160
Size: 872 6952 1606
Fragments arranged by size:
6952 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9430
AvaI C'yCGr_G
Cuts at: 1870 1943 2177 2525 2946 3254 3394 3701 4339
Size: 73 234 348 421 308 140 307 638
Cuts at: 4339 5220 5252 5274 5770 5793 6568 6707 6755
Size: 881 32 22 496 23 775 139 48
Cuts at: 6755 1870
Size: 4545
Fragments arranged by size:
4545 881 775 638 496 421 348 308
307 234 140 139 73 48 32 23
22
AvrII C'CTAG_G
Cuts at: 2374 2374
Size: 9430
BamHI G'GATC_C
Cuts at: 5324 6760 7127 5324
Size: 1436 367 7627
Fragments arranged by size:
7627 1436 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2044 2094 2201 2877 2951
Size: 325 217 627 257 50 107 676 74
Cuts at: 2951 3200 3694 4142 4410 4851 5303 5634 6751
Size: 249 494 448 268 441 452 331 1117
Cuts at: 6751 8206 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1117 676 627 494 452 448
441 331 325 268 257 249 217 107
74 50
BanII G_rGCy'C
Cuts at: 1391 2221 2321 2481 2672 2947 3030 3560 3584
Size: 830 100 160 191 275 83 530 24
Cuts at: 3584 3605 3624 3819 4196 4404 4622 4775 5221
Size: 21 19 195 377 208 218 153 446
Cuts at: 5221 5545 5779 6566 6749 1391
Size: 324 234 787 183 4072
Fragments arranged by size:
4072 830 787 530 446 377 324 275
234 218 208 195 191 183 160 153
100 83 24 21 19
BbeI G_GCGC'C
Cuts at: 2098 2205 2098
Size: 107 9323
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2516 3615 5339 5808 5840 6137 928
Size: 971 617 1099 1724 469 32 297 4221
Fragments arranged by size:
4221 1724 1099 971 617 469 297 32
BbvCI CC'TCA_GC
Cuts at: 3506 3728 6251 3506
Size: 222 2523 6685
Fragments arranged by size:
6685 2523 222
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4219 4253 6698 6732 7621 7655 1903
Size: 34 2282 34 2445 34 889 34 3678
Fragments arranged by size:
3678 2445 2282 889 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4067 7322 8532 4067
Size: 3255 1210 4965
Fragments arranged by size:
4965 3255 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9430
BfrBI ATG'CAT
Cuts at: 4593 4593
Size: 9430
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3780 4341
Size: 107 122 71 631 605 337 1771 561
Cuts at: 4341 5477 5762 5867 8041 136
Size: 1136 285 105 2174 1525
Fragments arranged by size:
2174 1771 1525 1136 631 605 561 337
285 122 107 105 71
BglII A'GATC_T
Cuts at: 9425 9425
Size: 9430
BlpI GC'TnA_GC
Cuts at: 6375 6395 6847 6375
Size: 20 452 8958
Fragments arranged by size:
8958 452 20
Bme1580I G_kGCm'C
Cuts at: 1790 2947 2954 3699 4196 4413 4814 5308 5317
Size: 1157 7 745 497 217 401 494 9
Cuts at: 5317 5488 5545 6538 7491 8647 1790
Size: 171 57 993 953 1156 2573
Fragments arranged by size:
2573 1157 1156 993 953 745 497 494
401 217 171 57 9 7
BmgBI CAC'GTC
Cuts at: 1318 2854 3850 1318
Size: 1536 996 6898
Fragments arranged by size:
6898 1536 996
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3133 3478 4643
Size: 416 53 83 399 24 1716 345 1165
Cuts at: 4643 4778 4922 4924 5436 7161 8119 442
Size: 135 144 2 512 1725 958 1753
Fragments arranged by size:
1753 1725 1716 1165 958 512 416 399
345 144 135 83 53 24 2
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9430
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2654 2753 3167 4712 5255 5378 5390 5441
Size: 1415 99 414 1545 543 123 12 51
Cuts at: 5441 5609 5645 5849 5867 6163 6386 6458 6620
Size: 168 36 204 18 296 223 72 162
Cuts at: 6620 6704 8090 1239
Size: 84 1386 2579
Fragments arranged by size:
2579 1545 1415 1386 543 414 296 223
204 168 162 123 99 84 72 51
36 18 12
Bpu10I CC'TnA_GC
Cuts at: 3506 3599 3728 3818 6251 3506
Size: 93 129 90 2433 6685
Fragments arranged by size:
6685 2433 129 93 90
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 7555 8420 8718 7555
Size: 865 298 8267
Fragments arranged by size:
8267 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3642 3830 5603 6446 8093 882
Size: 617 2143 188 1773 843 1647 2219
Fragments arranged by size:
2219 2143 1773 1647 843 617 188
BsaAI yAC'GTr
Cuts at: 493 1204 2163 2197 2397 7221 493
Size: 711 959 34 200 4824 2702
Fragments arranged by size:
4824 2702 959 711 200 34
BsaBI GATnn'nnATC
Cuts at: 9424 9424
Size: 9430
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2095 2202 2238 5187
Size: 53 83 186 936 562 107 36 2949
Cuts at: 5187 6323 6341 7237 7619 8254 275
Size: 1136 18 896 382 635 1451
Fragments arranged by size:
2949 1451 1136 936 896 635 562 382
186 107 83 53 36 18
BsaWI w'CCGG_w
Cuts at: 7863 8261 8535 8682 9093 7863
Size: 398 274 147 411 8200
Fragments arranged by size:
8200 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2252 2282 2483 2513 4307
Size: 30 165 30 849 30 201 30 1794
Cuts at: 4307 4337 4772 4802 5618 5648 1178
Size: 30 435 30 816 30 4960
Fragments arranged by size:
4960 1794 849 816 435 201 165 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2678 2696 3629 3632 3878 5084 5399 1186
Size: 1492 18 933 3 246 1206 315 5217
Fragments arranged by size:
5217 1492 1206 933 315 246 18 3
BseYI C'CCAG_C
Cuts at: 2026 2250 2645 3083 3932 4396 4416 4858 5164
Size: 224 395 438 849 464 20 442 306
Cuts at: 5164 5343 5613 6154 6327 6389 6478 6505 6559
Size: 179 270 541 173 62 89 27 54
Cuts at: 6559 6612 8633 9129 2026
Size: 53 2021 496 2327
Fragments arranged by size:
2327 2021 849 541 496 464 442 438
395 306 270 224 179 173 89 62
54 53 27 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2360 2557 3277 4108 4493 4671 4978
Size: 573 624 197 720 831 385 178 307
Cuts at: 4978 5323 5741 5918 6680 1163
Size: 345 418 177 762 3913
Fragments arranged by size:
3913 831 762 720 624 573 418 385
345 307 197 178 177
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2058 2521 6880 7641 8669 1540
Size: 275 186 57 463 4359 761 1028 2301
Fragments arranged by size:
4359 2301 1028 761 463 275 186 57
BsiHKAI G_wGCw'C
Cuts at: 2306 2769 3030 3567 3624 3819 4069 4539 4814
Size: 463 261 537 57 195 250 470 275
Cuts at: 4814 4907 5779 7491 7576 8647 2306
Size: 93 872 1712 85 1071 3089
Fragments arranged by size:
3089 1712 1071 872 537 470 463 275
261 250 195 93 85 57
BsmI GAATG_Cn'
Cuts at: 3014 6941 7034 3014
Size: 3927 93 5410
Fragments arranged by size:
5410 3927 93
BsmBI CGTCTCn'nnnn_
Cuts at: 5421 5421
Size: 9430
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2221 2306 2321 2481 2672 2769 2947
Size: 399 431 85 15 160 191 97 178
Cuts at: 2947 2954 3030 3560 3567 3584 3605 3624 3699
Size: 7 76 530 7 17 21 19 75
Cuts at: 3699 3819 4069 4196 4404 4413 4539 4622 4775
Size: 120 250 127 208 9 126 83 153
Cuts at: 4775 4814 4907 5221 5308 5317 5488 5545 5779
Size: 39 93 314 87 9 171 57 234
Cuts at: 5779 6538 6566 6749 7491 7576 8647 1391
Size: 759 28 183 742 85 1071 2174
Fragments arranged by size:
2174 1071 759 742 530 431 399 314
250 234 208 191 183 178 171 160
153 127 126 120 97 93 87 85
85 83 76 75 57 39 28 21
19 17 15 9 9 7 7
BspEI T'CCGG_A
Cuts at: 9093 9093
Size: 9430
BspHI T'CATG_A
Cuts at: 7319 9049 7319
Size: 1730 7700
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2547 2802 3212 3417 3837 4823 4890 5214
Size: 1703 255 410 205 420 986 67 324
Cuts at: 5214 5345 6322 6718 6785 844
Size: 131 977 396 67 3489
Fragments arranged by size:
3489 1703 986 977 420 410 396 324
255 205 131 67 67
BsrBI CCG'CTC
Cuts at: 1394 1823 3000 6259 6379 7317 1394
Size: 429 1177 3259 120 938 3507
Fragments arranged by size:
3507 3259 1177 938 429 120
BsrDI GCAATG_nn'
Cuts at: 65 1248 2221 5045 6074 7925 8099 65
Size: 1183 973 2824 1029 1851 174 1396
Fragments arranged by size:
2824 1851 1396 1183 1029 973 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2057 2113 2520 2640 2721 2874 2894
Size: 110 247 56 407 120 81 153 20
Cuts at: 2894 3941 8074 8261 1700
Size: 1047 4133 187 2869
Fragments arranged by size:
4133 2869 1047 407 247 187 153 120
110 81 56 20
BsrGI T'GTAC_A
Cuts at: 96 3721 96
Size: 3625 5805
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9430
BssSI C'ACGA_G
Cuts at: 2969 4937 6121 7490 8502 2969
Size: 1968 1184 1369 1012 3897
Fragments arranged by size:
3897 1968 1369 1184 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3291 4599 4704 5031 5531 6625 6703 794
Size: 2497 1308 105 327 500 1094 78 3521
Fragments arranged by size:
3521 2497 1308 1094 500 327 105 78
BstBI TT'CG_AA
Cuts at: 8234 8234
Size: 9430
BstEII G'GTnAC_C
Cuts at: 1184 2689 4523 1184
Size: 1505 1834 6091
Fragments arranged by size:
6091 1834 1505
BstXI CCAn_nnnn'nTGG
Cuts at: 3083 3550 4008 4465 3083
Size: 467 458 457 8048
Fragments arranged by size:
8048 467 458 457
BstYI r'GATC_y
Cuts at: 1972 4164 5324 6414 6630 6660 6760 7127 7512
Size: 2192 1160 1090 216 30 100 367 385
Cuts at: 7512 7529 8981 9067 9079 9425 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
2192 1977 1452 1160 1090 385 367 346
216 100 86 30 17 12
Bsu36I CC'TnA_GG
Cuts at: 3761 5430 6548 3761
Size: 1669 1118 6643
Fragments arranged by size:
6643 1669 1118
BtgI C'CryG_G
Cuts at: 513 1065 2004 2048 2205 2325 2471 2939 3046
Size: 552 939 44 157 120 146 468 107
Cuts at: 3046 3131 3219 4464 4518 513
Size: 85 88 1245 54 5425
Fragments arranged by size:
5425 1245 939 552 468 157 146 120
107 88 85 54 44
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3204 487
Size: 683 56 1978 6713
Fragments arranged by size:
6713 1978 683 56
BtsI GCAGTG_nn'
Cuts at: 2998 3064 3343 3477 3860 4330 4492 5029 5370
Size: 66 279 134 383 470 162 537 341
Cuts at: 5370 5529 6946 7740 7760 2998
Size: 159 1417 794 20 4668
Fragments arranged by size:
4668 1417 794 537 470 383 341 279
162 159 134 66 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9430
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2974 3009 529
Size: 35 2410 35 6950
Fragments arranged by size:
6950 2410 35 35
DraI TTT'AAA
Cuts at: 6734 6734
Size: 9430
DraIII CAC_nnn'GTG
Cuts at: 2310 3095 2310
Size: 785 8645
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8437 809
Size: 507 7121 1802
Fragments arranged by size:
7121 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2055 2518 2995
Size: 54 1425 50 275 18 225 463 477
Cuts at: 2995 3454 3983 4279 4489 5469 5712 5859 6216
Size: 459 529 296 210 980 243 147 357
Cuts at: 6216 6877 7766 9395 8
Size: 661 889 1629 43
Fragments arranged by size:
1629 1425 980 889 661 529 477 463
459 357 296 275 243 225 210 147
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2055 2518 6877 1537
Size: 275 243 463 4359 4090
Fragments arranged by size:
4359 4090 463 275 243
EarI CTCTTCn'nnn_
Cuts at: 1353 3035 5376 7360 1353
Size: 1682 2341 1984 3423
Fragments arranged by size:
3423 2341 1984 1682
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2360 3899 3967 6158 8017 8391
Size: 1064 138 771 1539 68 2191 1859 374
Cuts at: 8391 8537 387
Size: 146 1280
Fragments arranged by size:
2191 1859 1539 1280 1064 771 374 146
138 68
EcoICRI GAG'CTC
Cuts at: 3028 3622 3817 5777 3028
Size: 594 195 1960 6681
Fragments arranged by size:
6681 1960 594 195
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2429 2654 2753 3167 4712 5105 5255
Size: 393 797 225 99 414 1545 393 150
Cuts at: 5255 5378 5390 5441 5609 5645 5715 5820 5834
Size: 123 12 51 168 36 70 105 14
Cuts at: 5834 5849 5867 6163 6366 6386 6458 6620 6704
Size: 15 18 296 203 20 72 162 84
Cuts at: 6704 7493 8090 8877 9145 1239
Size: 789 597 787 268 1524
Fragments arranged by size:
1545 1524 797 789 787 597 414 393
393 296 268 225 203 168 162 150
123 105 99 84 72 70 51 36
20 18 15 14 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2378 3959 4846 5063 5433 5960 6250 6580 2378
Size: 1581 887 217 370 527 290 330 5228
Fragments arranged by size:
5228 1581 887 527 370 330 290 217
EcoO109I rG'GnC_Cy
Cuts at: 2755 3127 3399 3592 4100 4192 4390 5173 5438
Size: 372 272 193 508 92 198 783 265
Cuts at: 5438 5541 5542 5620 5824 6514 6573 2755
Size: 103 1 78 204 690 59 5612
Fragments arranged by size:
5612 783 690 508 372 272 265 204
198 193 103 92 78 59 1
EcoRI G'AATT_C
Cuts at: 6739 7117 6739
Size: 378 9052
FseI GG_CCGG'CC
Cuts at: 2524 2644 2524
Size: 120 9310
FspI TGC'GCA
Cuts at: 7936 7936
Size: 9430
HaeII r_GCGC'y
Cuts at: 1678 2039 2098 2107 2205 2233 2257 2499 2652
Size: 361 59 9 98 28 24 242 153
Cuts at: 2652 2914 3058 3103 3346 4360 4566 4920 8577
Size: 262 144 45 243 1014 206 354 3657
Cuts at: 8577 1678
Size: 2531
Fragments arranged by size:
3657 2531 1014 361 354 262 243 242
206 153 144 98 59 45 28 24
9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3146 3178 4181 4213 4469
Size: 32 135 32 1574 32 1003 32 256
Cuts at: 4469 4501 6443 6475 6593 6625 8071 8103 8145
Size: 32 1942 32 118 32 1446 32 42
Cuts at: 8145 8177 1373
Size: 32 2626
Fragments arranged by size:
2626 1942 1574 1446 1003 256 135 118
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2359 6774 7020 669
Size: 988 281 421 4415 246 3079
Fragments arranged by size:
4415 3079 988 421 281 246
HindIII A'AGCT_T
Cuts at: 748 6233 748
Size: 5485 3945
HpaI GTT'AAC
Cuts at: 7020 7020
Size: 9430
KasI G'GCGC_C
Cuts at: 2094 2201 2094
Size: 107 9323
KpnI G_GTAC'C
Cuts at: 5638 6755 5638
Size: 1117 8313
MfeI C'AATT_G
Cuts at: 7029 7029
Size: 9430
MluI A'CGCG_T
Cuts at: 9306 9306
Size: 9430
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2817 5151 5580 6340 8544 8728 1305
Size: 1512 2334 429 760 2204 184 2007
Fragments arranged by size:
2334 2204 2007 1512 760 429 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3456 4281 4491 5471 5714
Size: 54 1425 343 1624 825 210 980 243
Cuts at: 5714 5861 6218 10
Size: 147 357 3222
Fragments arranged by size:
3222 1624 1425 980 825 357 343 243
210 147 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2399 3069 4952 4954 6276 7388 7747
Size: 648 1233 670 1883 2 1322 1112 359
Cuts at: 7747 7906 518
Size: 159 2042
Fragments arranged by size:
2042 1883 1322 1233 1112 670 648 359
159 2
MspA1I CmG'CkG
Cuts at: 1606 2050 2207 2602 3083 3133 3246 3308 3428
Size: 444 157 395 481 50 113 62 120
Cuts at: 3428 3441 3655 3727 3883 4113 4396 4605 4704
Size: 13 214 72 156 230 283 209 99
Cuts at: 4704 4858 4992 5284 5310 5347 5402 5617 6559
Size: 154 134 292 26 37 55 215 942
Cuts at: 6559 6723 6846 7523 8671 8916 1606
Size: 164 123 677 1148 245 2120
Fragments arranged by size:
2120 1148 942 677 481 444 395 292
283 245 230 215 214 209 164 157
156 154 134 123 120 113 99 72
62 55 50 37 26 13
NaeI GCC'GGC
Cuts at: 2059 2522 2642 2059
Size: 463 120 8847
Fragments arranged by size:
8847 463 120
NarI GG'CG_CC
Cuts at: 2095 2202 2095
Size: 107 9323
NcoI C'CATG_G
Cuts at: 513 1065 2004 2325 2471 513
Size: 552 939 321 146 7472
Fragments arranged by size:
7472 939 552 321 146
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9430
NgoMIV G'CCGG_C
Cuts at: 2057 2520 2640 2057
Size: 463 120 8847
Fragments arranged by size:
8847 463 120
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9430
NotI GC'GGCC_GC
Cuts at: 6877 6877
Size: 9430
NsiI A_TGCA'T
Cuts at: 4595 4595
Size: 9430
NspI r_CATG'y
Cuts at: 1132 1229 2834 4597 4953 5399 6788 8333 1132
Size: 97 1605 1763 356 446 1389 1545 2229
Fragments arranged by size:
2229 1763 1605 1545 1389 446 356 97
PasI CC'CwG_GG
Cuts at: 1348 2672 3804 5142 1348
Size: 1324 1132 1338 5636
Fragments arranged by size:
5636 1338 1324 1132
PciI A'CATG_T
Cuts at: 8329 8329
Size: 9430
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5535 1108
Size: 4427 5003
PfoI T'CCnGG_A
Cuts at: 2928 4069 5232 6417 2928
Size: 1141 1163 1185 5941
Fragments arranged by size:
5941 1185 1163 1141
PmeI GTTT'AAAC
Cuts at: 6734 6734
Size: 9430
PmlI CAC'GTG
Cuts at: 2163 2197 2163
Size: 34 9396
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3806 3838 7499 7531 9012 9044 3806
Size: 32 3661 32 1481 32 4192
Fragments arranged by size:
4192 3661 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2755 3127 3399 3592 4100 4390 5438 5620 6573
Size: 372 272 193 508 290 1048 182 953
Cuts at: 6573 2755
Size: 5612
Fragments arranged by size:
5612 1048 953 508 372 290 272 193
182
PshAI GACnn'nnGTC
Cuts at: 1613 2187 3246 3387 3962 1613
Size: 574 1059 141 575 7081
Fragments arranged by size:
7081 1059 575 574 141
PsiI TTA'TAA
Cuts at: 7000 7000
Size: 9430
PspOMI G'GGCC_C
Cuts at: 2943 4192 5541 2943
Size: 1249 1349 6832
Fragments arranged by size:
6832 1349 1249
PspXI vC'TCGA_Gb
Cuts at: 5252 5252
Size: 9430
PstI C_TGCA'G
Cuts at: 830 2682 2993 3287 3414 3437 3834 4178 4700
Size: 1852 311 294 127 23 397 344 522
Cuts at: 4700 4901 5289 6048 6558 6630 6782 830
Size: 201 388 759 510 72 152 3478
Fragments arranged by size:
3478 1852 759 522 510 397 388 344
311 294 201 152 127 72 23
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9430
PvuII CAG'CTG
Cuts at: 3083 3428 3441 3727 4113 4605 4858 5284 5347
Size: 345 13 286 386 492 253 426 63
Cuts at: 5347 5402 6559 6723 3083
Size: 55 1157 164 5790
Fragments arranged by size:
5790 1157 492 426 386 345 286 253
164 63 55 13
SacI G_AGCT'C
Cuts at: 3030 3624 3819 5779 3030
Size: 594 195 1960 6681
Fragments arranged by size:
6681 1960 594 195
SacII CC_GC'GG
Cuts at: 2051 2208 3134 2051
Size: 157 926 8347
Fragments arranged by size:
8347 926 157
SalI G'TCGA_C
Cuts at: 1936 6772 1936
Size: 4836 4594
SanDI GG'GwC_CC
Cuts at: 3127 4100 4390 3127
Size: 973 290 8167
Fragments arranged by size:
8167 973 290
SapI GCTCTTCn'nnn_
Cuts at: 3035 3035
Size: 9430
SbfI CC_TGCA'GG
Cuts at: 3834 4901 6782 3834
Size: 1067 1881 6482
Fragments arranged by size:
6482 1881 1067
ScaI AGT'ACT
Cuts at: 7678 9390 7678
Size: 1712 7718
SexAI A'CCwGG_T
Cuts at: 2306 2306
Size: 9430
SfcI C'TryA_G
Cuts at: 826 1045 2491 2678 2989 3283 3410 3433 3830
Size: 219 1446 187 311 294 127 23 397
Cuts at: 3830 4174 4696 4897 5285 6044 6554 6626 6778
Size: 344 522 201 388 759 510 72 152
Cuts at: 6778 7913 8594 8785 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1446 1135 759 681 522 510 397
388 344 311 294 219 201 191 187
152 127 72 23
SfiI GGCCn_nnn'nGGCC
Cuts at: 5477 5477
Size: 9430
SfoI GGC'GCC
Cuts at: 2096 2203 2096
Size: 107 9323
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9430
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9430
SmaI CCC'GGG
Cuts at: 1872 2948 3256 3396 3703 4341 5276 5772 5795
Size: 1076 308 140 307 638 935 496 23
Cuts at: 5795 6570 6757 1872
Size: 775 187 4545
Fragments arranged by size:
4545 1076 935 775 638 496 308 307
187 140 23
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5252 7534 8435 8697 820
Size: 197 926 3309 2282 901 262 1553
Fragments arranged by size:
3309 2282 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 7221 493
Size: 6728 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9430
SphI G_CATG'C
Cuts at: 1132 2834 4597 5399 6788 1132
Size: 1702 1763 802 1389 3774
Fragments arranged by size:
3774 1763 1702 1389 802
SspI AAT'ATT
Cuts at: 5 52 7354 5
Size: 47 7302 2081
Fragments arranged by size:
7302 2081 47
StuI AGG'CCT
Cuts at: 1517 2153 4841 4963 6245 1517
Size: 636 2688 122 1282 4702
Fragments arranged by size:
4702 2688 1282 636 122
StyI C'CwwG_G
Cuts at: 513 1065 2004 2325 2374 2471 2760 3462 5119
Size: 552 939 321 49 97 289 702 1657
Cuts at: 5119 5177 6060 6219 6240 6869 513
Size: 58 883 159 21 629 3074
Fragments arranged by size:
3074 1657 939 883 702 629 552 321
289 159 97 58 49 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2344 4000 4612 4626 4779 5357 6374
Size: 1255 764 1656 612 14 153 578 1017
Cuts at: 6374 6509 7457 7474 7627 7812 8257 325
Size: 135 948 17 153 185 445 1498
Fragments arranged by size:
1656 1498 1255 1017 948 764 612 578
445 185 153 153 135 17 14
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3721 7676 9388
Size: 274 80 33 51 1443 1744 3955 1712
Cuts at: 9388 96
Size: 138
Fragments arranged by size:
3955 1744 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2252 2284 2924 2956 3286 3318 4307 4339 4772
Size: 32 640 32 330 32 989 32 433
Cuts at: 4772 4804 9390 9422 2252
Size: 32 4586 32 2260
Fragments arranged by size:
4586 2260 989 640 433 330 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 6095 6457 1316
Size: 4779 362 4289
Fragments arranged by size:
4779 4289 362
XbaI T'CTAG_A
Cuts at: 2716 6766 2716
Size: 4050 5380
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3425 4799 6016 3425
Size: 1374 1217 6839
Fragments arranged by size:
6839 1374 1217
XhoI C'TCGA_G
Cuts at: 1943 5252 1943
Size: 3309 6121
XmaI C'CCGG_G
Cuts at: 1870 2946 3254 3394 3701 4339 5274 5770 5793
Size: 1076 308 140 307 638 935 496 23
Cuts at: 5793 6568 6755 1870
Size: 775 187 4545
Fragments arranged by size:
4545 1076 935 775 638 496 308 307
187 140 23
XmnI GAAnn'nnTTC
Cuts at: 7559 7559
Size: 9430
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5188 7238 276
Size: 53 83 186 936 3654 2050 2468
Fragments arranged by size:
3654 2468 2050 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BaeI BplI BsiWI BstZ17I EcoRV FalI
FspAI NruI PacI PsrI RsrII SrfI SwaI