Restriction map for
pFN21AE6734
(Circular) (Six-base) MAPSORT of: pFN21AE6734.seq Check: 6538 from: 1 to: 7438
pFN21AE6734 7438 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2524 2524
Size: 7438
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5248 278
Size: 53 83 186 936 3712 2468
Fragments arranged by size:
3712 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2082 3987 4781 1937
Size: 145 1905 794 4594
Fragments arranged by size:
4594 1905 794 145
Acc65I G'GTAC_C
Cuts at: 2790 4759 2790
Size: 1969 5469
AclI AA'CG_TT
Cuts at: 1563 5565 5938 1563
Size: 4002 373 3063
Fragments arranged by size:
4002 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2233 2685 3203 3486 4075 4574 5501 6885
Size: 601 452 518 283 589 499 927 1384
Cuts at: 6885 7153 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 927 601 589 518 499 452
283 268
AfeI AGC'GCT
Cuts at: 3366 3366
Size: 7438
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7241
AflIII A'CryG_T
Cuts at: 1203 3294 3507 6337 7314 1203
Size: 2091 213 2830 977 1327
Fragments arranged by size:
2830 2091 1327 977 213
AgeI A'CCGG_T
Cuts at: 6269 6269
Size: 7438
AhdI GACnn_n'nnGTC
Cuts at: 4271 6167 4271
Size: 1896 5542
AleI CACnn'nnGTG
Cuts at: 1166 2889 1166
Size: 1723 5715
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7438
AlwNI CAG_nnn'CTG
Cuts at: 2870 4217 4532 4659 4678 5181 6753 2870
Size: 1347 315 127 19 503 1572 3555
Fragments arranged by size:
3555 1572 1347 503 315 127 19
ApaI G_GGCC'C
Cuts at: 2815 3256 3401 3659 4061 2815
Size: 441 145 258 402 6192
Fragments arranged by size:
6192 441 402 258 145
ApaLI G'TGCA_C
Cuts at: 5495 6651 5495
Size: 1156 6282
ApoI r'AATT_y
Cuts at: 1493 2177 3137 4747 4973 5125 7234 1493
Size: 684 960 1610 226 152 2109 1697
Fragments arranged by size:
2109 1697 1610 960 684 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5992 160
Size: 872 4960 1606
Fragments arranged by size:
4960 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7438
AvaI C'yCGr_G
Cuts at: 1870 1943 2706 2887 3069 3442 3720 4763 1870
Size: 73 763 181 182 373 278 1043 4545
Fragments arranged by size:
4545 1043 763 373 278 182 181 73
BamHI G'GATC_C
Cuts at: 4768 5135 4768
Size: 367 7071
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2062 2790 2891 2992 4759
Size: 325 217 627 275 728 101 101 1767
Cuts at: 4759 6214 618
Size: 1455 1842
Fragments arranged by size:
1842 1767 1455 728 627 325 275 217
101 101
BanII G_rGCy'C
Cuts at: 1391 2815 2850 3256 3401 3659 3744 4061 4189
Size: 1424 35 406 145 258 85 317 128
Cuts at: 4189 4690 4757 1391
Size: 501 67 4072
Fragments arranged by size:
4072 1424 501 406 317 258 145 128
85 67 35
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3290 3984 928
Size: 971 1391 694 4382
Fragments arranged by size:
4382 1391 971 694
BbvCI CC'TCA_GC
Cuts at: 2522 2522
Size: 7438
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5629 5663 1903
Size: 34 50 34 3608 34 3678
Fragments arranged by size:
3678 3608 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5330 6540 5330
Size: 1210 6228
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7438
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2014 2625 6049
Size: 107 122 71 631 605 342 611 3424
Cuts at: 6049 136
Size: 1525
Fragments arranged by size:
3424 1525 631 611 605 342 122 107
71
BglII A'GATC_T
Cuts at: 3534 7433 3534
Size: 3899 3539
BlpI GC'TnA_GC
Cuts at: 2954 4855 2954
Size: 1901 5537
Bme1580I G_kGCm'C
Cuts at: 1790 2067 2815 2896 2997 3256 3401 3659 4061
Size: 277 748 81 101 259 145 258 402
Cuts at: 4061 5499 6655 1790
Size: 1438 1156 2573
Fragments arranged by size:
2573 1438 1156 748 402 277 259 258
145 101 81
BmgBI CAC'GTC
Cuts at: 1318 2047 2272 4084 1318
Size: 729 225 1812 4672
Fragments arranged by size:
4672 1812 729 225
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5169 6127 442
Size: 416 53 83 399 24 3752 958 1753
Fragments arranged by size:
3752 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7438
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3060 3553 3060
Size: 493 6945
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2141 2486 2750 3389 3635 3699 3812 6098
Size: 902 345 264 639 246 64 113 2286
Cuts at: 6098 1239
Size: 2579
Fragments arranged by size:
2579 2286 902 639 345 264 246 113
64
Bpu10I CC'TnA_GC
Cuts at: 2522 4013 4679 2522
Size: 1491 666 5281
Fragments arranged by size:
5281 1491 666
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3229 5563 6428 6726 3229
Size: 2334 865 298 3941
Fragments arranged by size:
3941 2334 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3031 3295 4163 4258 6101 882
Size: 617 1532 264 868 95 1843 2219
Fragments arranged by size:
2219 1843 1532 868 617 264 95
BsaAI yAC'GTr
Cuts at: 493 1204 2100 5229 493
Size: 711 896 3129 2702
Fragments arranged by size:
3129 2702 896 711
BsaBI GATnn'nnATC
Cuts at: 7432 7432
Size: 7438
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3006 5245 5627 6262
Size: 53 83 186 936 1473 2239 382 635
Cuts at: 6262 275
Size: 1451
Fragments arranged by size:
2239 1473 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5871 6269 6543 6690 7101 5871
Size: 398 274 147 411 6208
Fragments arranged by size:
6208 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3104 3134 1178
Size: 30 165 30 1701 30 5482
Fragments arranged by size:
5482 1701 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2420 2606 2687 3036 3545 3569 3890 4388
Size: 1234 186 81 349 509 24 321 498
Cuts at: 4388 1186
Size: 4236
Fragments arranged by size:
4236 1234 509 498 349 321 186 81
24
BseYI C'CCAG_C
Cuts at: 2601 2808 3339 3349 3563 4257 6641 7137 2601
Size: 207 531 10 214 694 2384 496 2902
Fragments arranged by size:
2902 2384 694 531 496 214 207 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2069 2660 1163
Size: 573 333 591 5941
Fragments arranged by size:
5941 591 573 333
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4888 5649 6677 1540
Size: 275 186 2887 761 1028 2301
Fragments arranged by size:
2887 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2412 4396 5499 5584 6655 2412
Size: 1984 1103 85 1071 3195
Fragments arranged by size:
3195 1984 1103 1071 85
BsmI GAATG_Cn'
Cuts at: 4949 5042 4949
Size: 93 7345
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2067 2412 2815 2850 2896 2997 3256
Size: 399 277 345 403 35 46 101 259
Cuts at: 3256 3401 3659 3744 4061 4189 4396 4690 4757
Size: 145 258 85 317 128 207 294 67
Cuts at: 4757 5499 5584 6655 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 403 399 345 317 294
277 259 258 207 145 128 101 85
85 67 46 35
BspEI T'CCGG_A
Cuts at: 7101 7101
Size: 7438
BspHI T'CATG_A
Cuts at: 5327 7057 5327
Size: 1730 5708
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2524 3090 3876 4793 844
Size: 1680 566 786 917 3489
Fragments arranged by size:
3489 1680 917 786 566
BsrBI CCG'CTC
Cuts at: 1394 1823 3619 3625 3631 4327 4683 5325 1394
Size: 429 1796 6 6 696 356 642 3507
Fragments arranged by size:
3507 1796 696 642 429 356 6 6
BsrDI GCAATG_nn'
Cuts at: 65 1248 5933 6107 65
Size: 1183 4685 174 1396
Fragments arranged by size:
4685 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2130 4490 4735 6082 6269 1700
Size: 110 320 2360 245 1347 187 2869
Fragments arranged by size:
2869 2360 1347 320 245 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7438
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7438
BssSI C'ACGA_G
Cuts at: 3329 5498 6510 3329
Size: 2169 1012 4257
Fragments arranged by size:
4257 2169 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7438
BstBI TT'CG_AA
Cuts at: 6242 6242
Size: 7438
BstEII G'GTnAC_C
Cuts at: 1184 2435 3334 1184
Size: 1251 899 5288
Fragments arranged by size:
5288 1251 899
BstXI CCAn_nnnn'nTGG
Cuts at: 2464 3313 4438 2464
Size: 849 1125 5464
Fragments arranged by size:
5464 1125 849
BstYI r'GATC_y
Cuts at: 1972 3534 4213 4768 5135 5520 5537 6989 7075
Size: 1562 679 555 367 385 17 1452 86
Cuts at: 7075 7087 7433 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1562 1452 679 555 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2382 3488 3924 3969 4001 2382
Size: 1106 436 45 32 5819
Fragments arranged by size:
5819 1106 436 45 32
BtgI C'CryG_G
Cuts at: 513 1065 2012 2184 2457 3393 3452 3575 4481
Size: 552 947 172 273 936 59 123 906
Cuts at: 4481 4575 513
Size: 94 3376
Fragments arranged by size:
3376 947 936 906 552 273 172 123
94 59
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3115 487
Size: 683 56 1889 4810
Fragments arranged by size:
4810 1889 683 56
BtsI GCAGTG_nn'
Cuts at: 2583 4660 4954 5748 5768 2583
Size: 2077 294 794 20 4253
Fragments arranged by size:
4253 2077 794 294 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7438
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3250 3285 529
Size: 35 2686 35 4682
Fragments arranged by size:
4682 2686 35 35
DraI TTT'AAA
Cuts at: 4742 4742
Size: 7438
DraIII CAC_nnn'GTG
Cuts at: 3334 4352 3334
Size: 1018 6420
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2276 2459 6445 809
Size: 507 960 183 3986 1802
Fragments arranged by size:
3986 1802 960 507 183
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3258 3799 3813
Size: 54 1425 50 275 18 1428 541 14
Cuts at: 3813 4123 4572 4578 4885 5774 7403 8
Size: 310 449 6 307 889 1629 43
Fragments arranged by size:
1629 1428 1425 889 541 449 310 307
275 54 50 43 18 14 6
EagI C'GGCC_G
Cuts at: 1537 1812 4885 1537
Size: 275 3073 4090
Fragments arranged by size:
4090 3073 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2252 2664 3111 4315 5368 1353
Size: 899 412 447 1204 1053 3423
Fragments arranged by size:
3423 1204 1053 899 447 412
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6025 6399 6545 387
Size: 1064 138 4436 374 146 1280
Fragments arranged by size:
4436 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2141 2233 2486 2685 2750 3203 3389
Size: 393 509 92 253 199 65 453 186
Cuts at: 3389 3486 3635 3699 3812 4075 4574 5501 6098
Size: 97 149 64 113 263 499 927 597
Cuts at: 6098 6885 7153 1239
Size: 787 268 1524
Fragments arranged by size:
1524 927 787 597 509 499 453 393
268 263 253 199 186 149 113 97
92 65 64
EcoNI CCTnn'n_nnAGG
Cuts at: 3491 3872 3491
Size: 381 7057
EcoO109I rG'GnC_Cy
Cuts at: 2203 2314 2386 2605 2812 2867 3253 3398 3655
Size: 111 72 219 207 55 386 145 257
Cuts at: 3655 3656 4057 4058 4118 4675 2203
Size: 1 401 1 60 557 4966
Fragments arranged by size:
4966 557 401 386 257 219 207 145
111 72 60 55 1 1
EcoRI G'AATT_C
Cuts at: 3137 4747 5125 3137
Size: 1610 378 5450
Fragments arranged by size:
5450 1610 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2263 2263
Size: 7438
FspI TGC'GCA
Cuts at: 5944 5944
Size: 7438
HaeII r_GCGC'y
Cuts at: 1678 2137 3368 6585 1678
Size: 459 1231 3217 2531
Fragments arranged by size:
3217 2531 1231 459
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2429 2461 3052 3084 4198
Size: 32 135 32 857 32 591 32 1114
Cuts at: 4198 4205 4230 4237 4257 4289 6079 6111 6153
Size: 7 25 7 20 32 1790 32 42
Cuts at: 6153 6185 1373
Size: 32 2626
Fragments arranged by size:
2626 1790 1114 857 591 135 42 32
32 32 32 32 32 32 25 20
7 7
HincII GTy'rAC
Cuts at: 669 1657 1938 3988 4782 5028 669
Size: 988 281 2050 794 246 3079
Fragments arranged by size:
3079 2050 988 794 281 246
HindIII A'AGCT_T
Cuts at: 748 4099 748
Size: 3351 4087
HpaI GTT'AAC
Cuts at: 5028 5028
Size: 7438
KpnI G_GTAC'C
Cuts at: 2794 4763 2794
Size: 1969 5469
MfeI C'AATT_G
Cuts at: 2295 5037 2295
Size: 2742 4696
MluI A'CGCG_T
Cuts at: 3294 7314 3294
Size: 4020 3418
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2151 3579 4026 4455 4740 6552 6736 1305
Size: 846 1428 447 429 285 1812 184 2007
Fragments arranged by size:
2007 1812 1428 846 447 429 285 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3801 3815 10
Size: 54 1425 343 1969 14 3633
Fragments arranged by size:
3633 1969 1425 343 54 14
MslI CAynn'nnrTG
Cuts at: 518 1166 2889 5396 5755 5914 518
Size: 648 1723 2507 359 159 2042
Fragments arranged by size:
2507 2042 1723 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2014 2109 2215 2326 3577 3772 4523 4854
Size: 408 95 106 111 1251 195 751 331
Cuts at: 4854 5531 6679 6924 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1251 1148 751 677 408 331 245
195 111 106 95
NcoI C'CATG_G
Cuts at: 513 1065 2184 2457 3452 4481 513
Size: 552 1119 273 995 1029 3470
Fragments arranged by size:
3470 1119 1029 995 552 273
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7438
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7438
NotI GC'GGCC_GC
Cuts at: 4885 4885
Size: 7438
NspI r_CATG'y
Cuts at: 1132 1229 3511 4796 6341 1132
Size: 97 2282 1285 1545 2229
Fragments arranged by size:
2282 2229 1545 1285 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7438
PciI A'CATG_T
Cuts at: 3507 6337 3507
Size: 2830 4608
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2870 4084 1108
Size: 1762 1214 4462
Fragments arranged by size:
4462 1762 1214
PfoI T'CCnGG_A
Cuts at: 2265 2463 4614 4638 2265
Size: 198 2151 24 5065
Fragments arranged by size:
5065 2151 198 24
PmeI GTTT'AAAC
Cuts at: 4742 4742
Size: 7438
PmlI CAC'GTG
Cuts at: 2100 2100
Size: 7438
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5507 5539 7020 7052 5507
Size: 32 1481 32 5893
Fragments arranged by size:
5893 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2203 2386 2605 4675 2203
Size: 183 219 2070 4966
Fragments arranged by size:
4966 2070 219 183
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7438
PsiI TTA'TAA
Cuts at: 5008 5008
Size: 7438
PspOMI G'GGCC_C
Cuts at: 2811 3252 3397 3655 4057 2811
Size: 441 145 258 402 6192
Fragments arranged by size:
6192 441 402 258 145
PspXI vC'TCGA_Gb
Cuts at: 3069 3069
Size: 7438
PstI C_TGCA'G
Cuts at: 830 2364 2661 4184 4528 4790 830
Size: 1534 297 1523 344 262 3478
Fragments arranged by size:
3478 1534 1523 344 297 262
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7438
PvuII CAG'CTG
Cuts at: 2326 3772 4523 2326
Size: 1446 751 5241
Fragments arranged by size:
5241 1446 751
SacII CC_GC'GG
Cuts at: 2015 3578 2015
Size: 1563 5875
SalI G'TCGA_C
Cuts at: 1936 3986 4780 1936
Size: 2050 794 4594
Fragments arranged by size:
4594 2050 794
SanDI GG'GwC_CC
Cuts at: 2605 2605
Size: 7438
SbfI CC_TGCA'GG
Cuts at: 4790 4790
Size: 7438
ScaI AGT'ACT
Cuts at: 5686 7398 5686
Size: 1712 5726
SfcI C'TryA_G
Cuts at: 826 1045 2137 2360 2657 4180 4524 4786 5921
Size: 219 1092 223 297 1523 344 262 1135
Cuts at: 5921 6602 6793 826
Size: 681 191 1471
Fragments arranged by size:
1523 1471 1135 1092 681 344 297 262
223 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7438
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7438
SmaI CCC'GGG
Cuts at: 1872 2889 3722 4765 1872
Size: 1017 833 1043 4545
Fragments arranged by size:
4545 1043 1017 833
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3069 3208 5542 6443 6705 820
Size: 197 926 1126 139 2334 901 262 1553
Fragments arranged by size:
2334 1553 1126 926 901 262 197 139
SnaBI TAC'GTA
Cuts at: 493 5229 493
Size: 4736 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7438
SphI G_CATG'C
Cuts at: 1132 4796 1132
Size: 3664 3774
SspI AAT'ATT
Cuts at: 5 52 5362 5
Size: 47 5310 2081
Fragments arranged by size:
5310 2081 47
StuI AGG'CCT
Cuts at: 1517 3075 3180 4018 1517
Size: 1558 105 838 4937
Fragments arranged by size:
4937 1558 838 105
StyI C'CwwG_G
Cuts at: 513 1065 2184 2457 3452 3816 4481 4877 513
Size: 552 1119 273 995 364 665 396 3074
Fragments arranged by size:
3074 1119 995 665 552 396 364 273
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5465 5482 5635 5820 6265 325
Size: 1255 3885 17 153 185 445 1498
Fragments arranged by size:
3885 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2679 3276 5684
Size: 274 80 33 51 1443 702 597 2408
Cuts at: 5684 7396 96
Size: 1712 138
Fragments arranged by size:
2408 1712 1443 702 597 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2762 2794 7398 7430 2762
Size: 32 4604 32 2770
Fragments arranged by size:
4604 2770 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3983 1316
Size: 2667 4771
XbaI T'CTAG_A
Cuts at: 4774 4774
Size: 7438
XhoI C'TCGA_G
Cuts at: 1943 3069 1943
Size: 1126 6312
XmaI C'CCGG_G
Cuts at: 1870 2887 3720 4763 1870
Size: 1017 833 1043 4545
Fragments arranged by size:
4545 1043 1017 833
XmnI GAAnn'nnTTC
Cuts at: 3905 5567 3905
Size: 1662 5776
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5246 276
Size: 53 83 186 936 3712 2468
Fragments arranged by size:
3712 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII SacII SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI
BstZ17I EcoICRI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PsrI RsrII SacI SapI
SexAI SfiI SfoI SrfI SwaI XcmI