Restriction map for
pFN21AE6732
(Circular) (Six-base) MAPSORT of: pFN21AE6732.seq Check: 8357 from: 1 to: 8107
pFN21AE6732 8107 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3760 3922 4748 3760
Size: 162 826 7119
Fragments arranged by size:
7119 826 162
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5917 278
Size: 53 83 186 936 4381 2468
Fragments arranged by size:
4381 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4312 5450 1937
Size: 2375 1138 4594
Fragments arranged by size:
4594 2375 1138
Acc65I G'GTAC_C
Cuts at: 5428 5428
Size: 8107
AclI AA'CG_TT
Cuts at: 1563 6234 6607 1563
Size: 4671 373 3063
Fragments arranged by size:
4671 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2093 2315 3477 3935 4439 4566 6170 7554
Size: 461 222 1162 458 504 127 1604 1384
Cuts at: 7554 7822 1632
Size: 268 1917
Fragments arranged by size:
1917 1604 1384 1162 504 461 458 268
222 127
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7910
AflIII A'CryG_T
Cuts at: 1203 3115 7006 7983 1203
Size: 1912 3891 977 1327
Fragments arranged by size:
3891 1912 1327 977
AgeI A'CCGG_T
Cuts at: 6938 6938
Size: 8107
AhdI GACnn_n'nnGTC
Cuts at: 6836 6836
Size: 8107
AleI CACnn'nnGTG
Cuts at: 1166 2217 1166
Size: 1051 7056
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2281 3080 1184
Size: 1097 799 6211
Fragments arranged by size:
6211 1097 799
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2451 2483 2787 2819 2451
Size: 32 304 32 7739
Fragments arranged by size:
7739 304 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2171 4262 5850 7422 2171
Size: 2091 1588 1572 2856
Fragments arranged by size:
2856 2091 1588 1572
ApaLI G'TGCA_C
Cuts at: 6164 7320 6164
Size: 1156 6951
ApoI r'AATT_y
Cuts at: 1493 2082 2158 2514 3407 3617 4661 4999 5224
Size: 589 76 356 893 210 1044 338 225
Cuts at: 5224 5260 5309 5320 5416 5642 5794 7903 1493
Size: 36 49 11 96 226 152 2109 1697
Fragments arranged by size:
2109 1697 1044 893 589 356 338 226
225 210 152 96 76 49 36 11
AseI AT'TA_AT
Cuts at: 160 1032 5156 6661 160
Size: 872 4124 1505 1606
Fragments arranged by size:
4124 1606 1505 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8107
AvaI C'yCGr_G
Cuts at: 1870 1943 5432 1870
Size: 73 3489 4545
Fragments arranged by size:
4545 3489 73
AvrII C'CTAG_G
Cuts at: 2065 4882 2065
Size: 2817 5290
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2417 2450 4574 4607 2417
Size: 33 2124 33 5917
Fragments arranged by size:
5917 2124 33 33
BamHI G'GATC_C
Cuts at: 4422 5355 5437 5804 4422
Size: 933 82 367 6725
Fragments arranged by size:
6725 933 367 82
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3809 5362 5377 5428 6883
Size: 325 217 627 2022 1553 15 51 1455
Cuts at: 6883 618
Size: 1842
Fragments arranged by size:
2022 1842 1553 1455 627 325 217 51
15
BanII G_rGCy'C
Cuts at: 1391 2933 5393 5426 1391
Size: 1542 2460 33 4072
Fragments arranged by size:
4072 2460 1542 33
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2474 3099 3201 3972 4618 4783 928
Size: 971 575 625 102 771 646 165 4252
Fragments arranged by size:
4252 971 771 646 625 575 165 102
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6298 6332 1903
Size: 34 4361 34 3678
Fragments arranged by size:
4361 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3321 3333 5999 7209 3321
Size: 12 2666 1210 4219
Fragments arranged by size:
4219 2666 1210 12
BclI T'GATC_A
Cuts at: 1548 3028 1548
Size: 1480 6627
BfrBI ATG'CAT
Cuts at: 5248 5248
Size: 8107
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6718 136
Size: 107 122 71 631 605 5046 1525
Fragments arranged by size:
5046 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3080 4092 8102 3080
Size: 1012 4010 3085
Fragments arranged by size:
4010 3085 1012
BlpI GC'TnA_GC
Cuts at: 5524 5524
Size: 8107
Bme1580I G_kGCm'C
Cuts at: 1790 6168 7324 1790
Size: 4378 1156 2573
Fragments arranged by size:
4378 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8107
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3321 3703 3818
Size: 416 53 83 399 24 1904 382 115
Cuts at: 3818 5838 6796 442
Size: 2020 958 1753
Fragments arranged by size:
2020 1904 1753 958 416 399 382 115
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8107
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4051 4051
Size: 8107
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2736 2814 3345 4046 4284 6767 1239
Size: 1497 78 531 701 238 2483 2579
Fragments arranged by size:
2579 2483 1497 701 531 238 78
Bpu10I CC'TnA_GC
Cuts at: 5385 5385
Size: 8107
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2612 2917 6232 7097 7395 2612
Size: 305 3315 865 298 3324
Fragments arranged by size:
3324 3315 865 305 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2194 3149 4698 6770 882
Size: 617 695 955 1549 2072 2219
Fragments arranged by size:
2219 2072 1549 955 695 617
BsaAI yAC'GTr
Cuts at: 493 1204 5898 493
Size: 711 4694 2702
Fragments arranged by size:
4694 2702 711
BsaBI GATnn'nnATC
Cuts at: 3321 8101 3321
Size: 4780 3327
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2697 5914 6296 6931
Size: 53 83 186 936 1164 3217 382 635
Cuts at: 6931 275
Size: 1451
Fragments arranged by size:
3217 1451 1164 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4106 6540 6938 7212 7359 7770 4106
Size: 2434 398 274 147 411 4443
Fragments arranged by size:
4443 2434 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2787 2817 1178
Size: 30 165 30 1384 30 6468
Fragments arranged by size:
6468 1384 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4011 1186
Size: 2825 5282
BseYI C'CCAG_C
Cuts at: 2762 3893 4353 7310 7806 2762
Size: 1131 460 2957 496 3063
Fragments arranged by size:
3063 2957 1131 496 460
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4146 1163
Size: 573 2410 5124
Fragments arranged by size:
5124 2410 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5557 6318 7346 1540
Size: 275 186 3556 761 1028 2301
Fragments arranged by size:
3556 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2211 2628 3361 4635 4938 6168 6253 7324 2211
Size: 417 733 1274 303 1230 85 1071 2994
Fragments arranged by size:
2994 1274 1230 1071 733 417 303 85
BsmI GAATG_Cn'
Cuts at: 5618 5711 5618
Size: 93 8014
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2211 2628 2933 3361 4635 4938 5393
Size: 399 421 417 305 428 1274 303 455
Cuts at: 5393 5426 6168 6253 7324 1391
Size: 33 742 85 1071 2174
Fragments arranged by size:
2174 1274 1071 742 455 428 421 417
399 305 303 85 33
BspEI T'CCGG_A
Cuts at: 7770 7770
Size: 8107
BspHI T'CATG_A
Cuts at: 3031 4797 5996 7726 3031
Size: 1766 1199 1730 3412
Fragments arranged by size:
3412 1766 1730 1199
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2122 2176 3760 3922 4748 5462 844
Size: 1278 54 1584 162 826 714 3489
Fragments arranged by size:
3489 1584 1278 826 714 162 54
BsrBI CCG'CTC
Cuts at: 1394 1823 5994 1394
Size: 429 4171 3507
Fragments arranged by size:
4171 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3143 3996 5190 6602 6776 65
Size: 1183 1895 853 1194 1412 174 1396
Fragments arranged by size:
1895 1412 1396 1194 1183 853 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6751 6938 1700
Size: 110 4941 187 2869
Fragments arranged by size:
4941 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3232 96
Size: 3136 4971
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8107
BssSI C'ACGA_G
Cuts at: 6167 7179 6167
Size: 1012 7095
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8107
BstBI TT'CG_AA
Cuts at: 6911 6911
Size: 8107
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8107
BstXI CCAn_nnnn'nTGG
Cuts at: 2219 3879 2219
Size: 1660 6447
BstYI r'GATC_y
Cuts at: 1972 2123 3080 4092 4422 4989 5355 5437 5804
Size: 151 957 1012 330 567 366 82 367
Cuts at: 5804 6189 6206 7658 7744 7756 8102 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1012 957 567 385 367 366
346 330 151 86 82 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2118 2180 3168 3764 4376 4586
Size: 552 939 114 62 988 596 612 210
Cuts at: 4586 513
Size: 4034
Fragments arranged by size:
4034 988 939 612 596 552 210 114
62
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2508 487
Size: 683 56 1282 6086
Fragments arranged by size:
6086 1282 683 56
BtsI GCAGTG_nn'
Cuts at: 2263 2426 3064 3211 5623 6417 6437 2263
Size: 163 638 147 2412 794 20 3933
Fragments arranged by size:
3933 2412 794 638 163 147 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8107
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8072
DraI TTT'AAA
Cuts at: 3544 3612 5049 5411 3544
Size: 68 1437 362 6240
Fragments arranged by size:
6240 1437 362 68
DraIII CAC_nnn'GTG
Cuts at: 3339 3853 3339
Size: 514 7593
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3401 7114 809
Size: 507 2085 3713 1802
Fragments arranged by size:
3713 2085 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2016 2050 3761
Size: 54 1425 50 275 18 186 34 1711
Cuts at: 3761 4529 5554 6443 8072 8
Size: 768 1025 889 1629 43
Fragments arranged by size:
1711 1629 1425 1025 889 768 275 186
54 50 43 34 18
EagI C'GGCC_G
Cuts at: 1537 1812 5554 1537
Size: 275 3742 4090
Fragments arranged by size:
4090 3742 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2731 6037 1353
Size: 1378 3306 3423
Fragments arranged by size:
3423 3306 1378
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6694 7068 7214 387
Size: 1064 138 5105 374 146 1280
Fragments arranged by size:
5105 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2093 2315 2736 2814 3345 3477 3935
Size: 393 461 222 421 78 531 132 458
Cuts at: 3935 4046 4284 4439 4566 6170 6767 7554 7822
Size: 111 238 155 127 1604 597 787 268
Cuts at: 7822 1239
Size: 1524
Fragments arranged by size:
1604 1524 787 597 531 461 458 421
393 268 238 222 155 132 127 111
78
EcoO109I rG'GnC_Cy
Cuts at: 2950 2950
Size: 8107
EcoRI G'AATT_C
Cuts at: 2082 2514 5416 5794 2082
Size: 432 2902 378 4395
Fragments arranged by size:
4395 2902 432 378
FspI TGC'GCA
Cuts at: 6613 6613
Size: 8107
HaeII r_GCGC'y
Cuts at: 1678 7254 1678
Size: 5576 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2161 2193 3026 3058 3798
Size: 32 135 32 589 32 833 32 740
Cuts at: 3798 3830 4392 4424 4508 4540 4664 4696 6748
Size: 32 562 32 84 32 124 32 2052
Cuts at: 6748 6780 6822 6854 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2052 833 740 589 562 135 124
84 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2095 3384 3495 5451 5697 669
Size: 988 281 157 1289 111 1956 246 3079
Fragments arranged by size:
3079 1956 1289 988 281 246 157 111
HindIII A'AGCT_T
Cuts at: 748 2227 748
Size: 1479 6628
HpaI GTT'AAC
Cuts at: 5697 5697
Size: 8107
KpnI G_GTAC'C
Cuts at: 5432 5432
Size: 8107
MfeI C'AATT_G
Cuts at: 3857 4490 5706 3857
Size: 633 1216 6258
Fragments arranged by size:
6258 1216 633
MluI A'CGCG_T
Cuts at: 7983 7983
Size: 8107
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2132 3001 5269 7221 7405 1305
Size: 827 869 2268 1952 184 2007
Fragments arranged by size:
2268 2007 1952 869 827 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2018 3763 4531 10
Size: 54 1425 343 186 1745 768 3586
Fragments arranged by size:
3586 1745 1425 768 343 186 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2217 3036 3530 3956 4306 4320 5121
Size: 648 1051 819 494 426 350 14 801
Cuts at: 5121 6065 6424 6583 518
Size: 944 359 159 2042
Fragments arranged by size:
2042 1051 944 819 801 648 494 426
359 350 159 14
MspA1I CmG'CkG
Cuts at: 1606 2293 4353 4813 5523 6200 7348 7593 1606
Size: 687 2060 460 710 677 1148 245 2120
Fragments arranged by size:
2120 2060 1148 710 687 677 460 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2180 3168 3764 4376 513
Size: 552 939 176 988 596 612 4244
Fragments arranged by size:
4244 988 939 612 596 552 176
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8107
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8107
NotI GC'GGCC_GC
Cuts at: 5554 5554
Size: 8107
NsiI A_TGCA'T
Cuts at: 5250 5250
Size: 8107
NspI r_CATG'y
Cuts at: 1132 1229 4172 5465 7010 1132
Size: 97 2943 1293 1545 2229
Fragments arranged by size:
2943 2229 1545 1293 97
PasI CC'CwG_GG
Cuts at: 1348 2650 1348
Size: 1302 6805
PciI A'CATG_T
Cuts at: 7006 7006
Size: 8107
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2535 1108
Size: 1427 6680
PfoI T'CCnGG_A
Cuts at: 3878 4216 4804 5351 3878
Size: 338 588 547 6634
Fragments arranged by size:
6634 588 547 338
PmeI GTTT'AAAC
Cuts at: 5411 5411
Size: 8107
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2787 2819 6176 6208 7689 7721 2787
Size: 32 3357 32 1481 32 3173
Fragments arranged by size:
3357 3173 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8107
PsiI TTA'TAA
Cuts at: 3377 5677 3377
Size: 2300 5807
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3615 3647 3615
Size: 32 8075
PstI C_TGCA'G
Cuts at: 830 5459 830
Size: 4629 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8107
PvuII CAG'CTG
Cuts at: 2293 4353 2293
Size: 2060 6047
SalI G'TCGA_C
Cuts at: 1936 5449 1936
Size: 3513 4594
SbfI CC_TGCA'GG
Cuts at: 5459 5459
Size: 8107
ScaI AGT'ACT
Cuts at: 6355 8067 6355
Size: 1712 6395
SfcI C'TryA_G
Cuts at: 826 1045 4151 5455 6590 7271 7462 826
Size: 219 3106 1304 1135 681 191 1471
Fragments arranged by size:
3106 1471 1304 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8107
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8107
SmaI CCC'GGG
Cuts at: 1872 5434 1872
Size: 3562 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2627 2932 6211 7112 7374 820
Size: 197 926 684 305 3279 901 262 1553
Fragments arranged by size:
3279 1553 926 901 684 305 262 197
SnaBI TAC'GTA
Cuts at: 493 5898 493
Size: 5405 2702
SpeI A'CTAG_T
Cuts at: 152 4303 152
Size: 4151 3956
SphI G_CATG'C
Cuts at: 1132 5465 1132
Size: 4333 3774
SspI AAT'ATT
Cuts at: 5 52 2359 2564 3132 3510 5071 6031 5
Size: 47 2307 205 568 378 1561 960 2081
Fragments arranged by size:
2307 2081 1561 960 568 378 205 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8107
StyI C'CwwG_G
Cuts at: 513 1065 2004 2065 2180 3168 3764 4376 4882
Size: 552 939 61 115 988 596 612 506
Cuts at: 4882 5546 513
Size: 664 3074
Fragments arranged by size:
3074 988 939 664 612 596 552 506
115 61
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4516 4618 6134 6151 6304 6489 6934
Size: 1255 2936 102 1516 17 153 185 445
Cuts at: 6934 325
Size: 1498
Fragments arranged by size:
2936 1516 1498 1255 445 185 153 102
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2322 3232 4029
Size: 274 80 33 51 1443 345 910 797
Cuts at: 4029 6353 8065 96
Size: 2324 1712 138
Fragments arranged by size:
2324 1712 1443 910 797 345 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4372 4404 4800 4832 8067 8099 4372
Size: 32 396 32 3235 32 4380
Fragments arranged by size:
4380 3235 396 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8107
XbaI T'CTAG_A
Cuts at: 2126 2138 5443 2126
Size: 12 3305 4790
Fragments arranged by size:
4790 3305 12
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2711 2972 3166 4289 2011
Size: 700 261 194 1123 5829
Fragments arranged by size:
5829 1123 700 261 194
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8107
XmaI C'CCGG_G
Cuts at: 1870 5432 1870
Size: 3562 4545
XmnI GAAnn'nnTTC
Cuts at: 4274 6236 4274
Size: 1962 6145
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5915 276
Size: 53 83 186 936 4381 2468
Fragments arranged by size:
4381 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI ApaI AscI BbeI BbvCI BsiWI BsmBI BstZ17I
Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI
PspXI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI