Restriction map for
pFN21AE6731
(Circular) (Six-base) MAPSORT of: pFN21AE6731.seq Check: 6125 from: 1 to: 8863
pFN21AE6731 8863 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3139 4054 3139
Size: 915 7948
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6673 278
Size: 53 83 186 936 5137 2468
Fragments arranged by size:
5137 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2424 2869 6206 1937
Size: 487 445 3337 4594
Fragments arranged by size:
4594 3337 487 445
Acc65I G'GTAC_C
Cuts at: 3515 6184 3515
Size: 2669 6194
AclI AA'CG_TT
Cuts at: 1563 6990 7363 1563
Size: 5427 373 3063
Fragments arranged by size:
5427 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2286 2775 3030 3173 3769 6926 8310 8578
Size: 654 489 255 143 596 3157 1384 268
Cuts at: 8578 1632
Size: 1917
Fragments arranged by size:
3157 1917 1384 654 596 489 268 255
143
AflII C'TTAA_G
Cuts at: 820 1017 3611 820
Size: 197 2594 6072
Fragments arranged by size:
6072 2594 197
AflIII A'CryG_T
Cuts at: 1203 3270 6023 7762 8739 1203
Size: 2067 2753 1739 977 1327
Fragments arranged by size:
2753 2067 1739 1327 977
AgeI A'CCGG_T
Cuts at: 7694 7694
Size: 8863
AhdI GACnn_n'nnGTC
Cuts at: 4168 7592 4168
Size: 3424 5439
AleI CACnn'nnGTG
Cuts at: 1166 2432 2981 3242 1166
Size: 1266 549 261 6787
Fragments arranged by size:
6787 1266 549 261
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2970 1184
Size: 1786 7077
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4371 4403 4371
Size: 32 8831
AlwNI CAG_nnn'CTG
Cuts at: 3083 3166 3388 3475 3797 4006 4136 5697 6606
Size: 83 222 87 322 209 130 1561 909
Cuts at: 6606 8178 3083
Size: 1572 3768
Fragments arranged by size:
3768 1572 1561 909 322 222 209 130
87 83
ApaI G_GGCC'C
Cuts at: 5851 6086 5851
Size: 235 8628
ApaLI G'TGCA_C
Cuts at: 2390 6920 8076 2390
Size: 4530 1156 3177
Fragments arranged by size:
4530 3177 1156
ApoI r'AATT_y
Cuts at: 1493 6172 6398 6550 8659 1493
Size: 4679 226 152 2109 1697
Fragments arranged by size:
4679 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7417 160
Size: 872 6385 1606
Fragments arranged by size:
6385 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8863
AvaI C'yCGr_G
Cuts at: 1870 1943 2232 4619 4721 6003 6078 6188 1870
Size: 73 289 2387 102 1282 75 110 4545
Fragments arranged by size:
4545 2387 1282 289 110 102 75 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4345 4378 4345
Size: 33 8830
BamHI G'GATC_C
Cuts at: 2332 2854 6193 6560 2332
Size: 522 3339 367 4635
Fragments arranged by size:
4635 3339 522 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2366 2397 3515 4431 4510
Size: 325 217 627 579 31 1118 916 79
Cuts at: 4510 4746 5343 5397 5542 6014 6184 7639 618
Size: 236 597 54 145 472 170 1455 1842
Fragments arranged by size:
1842 1455 1118 916 627 597 579 472
325 236 217 170 145 79 54 31
BanII G_rGCy'C
Cuts at: 1391 2244 3679 3924 4038 4620 4677 4728 4770
Size: 853 1435 245 114 582 57 51 42
Cuts at: 4770 5382 5851 6059 6077 6086 6182 1391
Size: 612 469 208 18 9 96 4072
Fragments arranged by size:
4072 1435 853 612 582 469 245 208
114 96 57 51 42 18 9
BbeI G_GCGC'C
Cuts at: 2370 2401 4750 2370
Size: 31 2349 6483
Fragments arranged by size:
6483 2349 31
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3630 928
Size: 971 1731 6161
Fragments arranged by size:
6161 1731 971
BbvCI CC'TCA_GC
Cuts at: 5648 5858 5648
Size: 210 8653
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4648 4682 7054 7088 1903
Size: 34 50 34 2627 34 2372 34 3678
Fragments arranged by size:
3678 2627 2372 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5584 6755 7965 5584
Size: 1171 1210 6482
Fragments arranged by size:
6482 1210 1171
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8863
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3199 4709 5551
Size: 107 122 71 631 605 1527 1510 842
Cuts at: 5551 7474 136
Size: 1923 1525
Fragments arranged by size:
1923 1527 1525 1510 842 631 605 122
107 71
BglII A'GATC_T
Cuts at: 2218 4425 5131 8858 2218
Size: 2207 706 3727 2223
Fragments arranged by size:
3727 2223 2207 706
BlpI GC'TnA_GC
Cuts at: 4769 6280 4769
Size: 1511 7352
Bme1580I G_kGCm'C
Cuts at: 1790 2394 2571 4486 4515 4572 4579 5033 5348
Size: 604 177 1915 29 57 7 454 315
Cuts at: 5348 5547 5686 5851 5931 6017 6086 6924 8080
Size: 199 139 165 80 86 69 838 1156
Cuts at: 8080 1790
Size: 2573
Fragments arranged by size:
2573 1915 1156 838 604 454 315 199
177 165 139 86 80 69 57 29
7
BmgBI CAC'GTC
Cuts at: 1318 3037 3765 1318
Size: 1719 728 6416
Fragments arranged by size:
6416 1719 728
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3040 3171 3438
Size: 416 53 83 399 24 1623 131 267
Cuts at: 3438 3637 3654 3759 4082 4302 4566 4574 5649
Size: 199 17 105 323 220 264 8 1075
Cuts at: 5649 6594 7552 442
Size: 945 958 1753
Fragments arranged by size:
1753 1623 1075 958 945 416 399 323
267 264 220 199 131 105 83 53
24 17 8
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8863
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2794 4849 5341 5401 5580 2794
Size: 2055 492 60 179 6077
Fragments arranged by size:
6077 2055 492 179 60
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2065 2623 2789 3105 3341 3350 3476 3857
Size: 826 558 166 316 236 9 126 381
Cuts at: 3857 4194 4634 4646 4857 5145 5164 5336 5371
Size: 337 440 12 211 288 19 172 35
Cuts at: 5371 5906 6043 7523 1239
Size: 535 137 1480 2579
Fragments arranged by size:
2579 1480 826 558 535 440 381 337
316 288 236 211 172 166 137 126
35 19 12 9
Bpu10I CC'TnA_GC
Cuts at: 5648 5858 5648
Size: 210 8653
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2609 2669 6988 7853 8151 2609
Size: 60 4319 865 298 3321
Fragments arranged by size:
4319 3321 865 298 60
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2233 3104 4523 7526 882
Size: 617 734 871 1419 3003 2219
Fragments arranged by size:
3003 2219 1419 871 734 617
BsaAI yAC'GTr
Cuts at: 493 1204 3271 6654 493
Size: 711 2067 3383 2702
Fragments arranged by size:
3383 2702 2067 711
BsaBI GATnn'nnATC
Cuts at: 2648 8857 2648
Size: 6209 2654
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2367 2398 4747 6670
Size: 53 83 186 936 834 31 2349 1923
Cuts at: 6670 7052 7687 275
Size: 382 635 1451
Fragments arranged by size:
2349 1923 1451 936 834 635 382 186
83 53 31
BsaWI w'CCGG_w
Cuts at: 2179 2919 5751 7296 7694 7968 8115 8526 2179
Size: 740 2832 1545 398 274 147 411 2516
Fragments arranged by size:
2832 2516 1545 740 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3078 3108 4317 4347 4371
Size: 30 165 30 1675 30 1209 30 24
Cuts at: 4371 4401 5187 5217 5416 5446 6085 6091 6115
Size: 30 786 30 199 30 639 6 24
Cuts at: 6115 6121 1178
Size: 6 3920
Fragments arranged by size:
3920 1675 1209 786 639 199 165 30
30 30 30 30 30 30 24 24
6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2098 3391 4313 4511 5099 5148 5259 5321
Size: 912 1293 922 198 588 49 111 62
Cuts at: 5321 5324 5351 1186
Size: 3 27 4698
Fragments arranged by size:
4698 1293 922 912 588 198 111 62
49 27 3
BseYI C'CCAG_C
Cuts at: 2360 3057 3260 3790 3923 4258 4395 4479 5074
Size: 697 203 530 133 335 137 84 595
Cuts at: 5074 5381 5496 5577 5587 5844 5940 8066 8562
Size: 307 115 81 10 257 96 2126 496
Cuts at: 8562 2360
Size: 2661
Fragments arranged by size:
2661 2126 697 595 530 496 335 307
257 203 137 133 115 96 84 81
10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3266 3557 3851 4610 5246 1163
Size: 573 1530 291 294 759 636 4780
Fragments arranged by size:
4780 1530 759 636 573 294 291
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2411 5037 6036 6313 7074 8102
Size: 275 186 410 2626 999 277 761 1028
Cuts at: 8102 1540
Size: 2301
Fragments arranged by size:
2626 2301 1028 999 761 410 277 275
186
BsiHKAI G_wGCw'C
Cuts at: 2394 4770 5746 5952 6924 7009 8080 2394
Size: 2376 976 206 972 85 1071 3177
Fragments arranged by size:
3177 2376 1071 976 972 206 85
BsmI GAATG_Cn'
Cuts at: 2567 4290 6374 6467 2567
Size: 1723 2084 93 4963
Fragments arranged by size:
4963 2084 1723 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3937 3937
Size: 8863
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2244 2394 2571 3679 3924 4038 4486
Size: 399 454 150 177 1108 245 114 448
Cuts at: 4486 4515 4572 4579 4620 4677 4728 4770 5033
Size: 29 57 7 41 57 51 42 263
Cuts at: 5033 5348 5382 5547 5686 5746 5851 5931 5952
Size: 315 34 165 139 60 105 80 21
Cuts at: 5952 6017 6059 6077 6086 6182 6924 7009 8080
Size: 65 42 18 9 96 742 85 1071
Cuts at: 8080 1391
Size: 2174
Fragments arranged by size:
2174 1108 1071 742 454 448 399 315
263 245 177 165 150 139 114 105
96 85 80 65 60 57 57 51
42 42 41 34 29 21 18 9
7
BspEI T'CCGG_A
Cuts at: 2919 5751 8526 2919
Size: 2832 2775 3256
Fragments arranged by size:
3256 2832 2775
BspHI T'CATG_A
Cuts at: 6752 8482 6752
Size: 1730 7133
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2025 2898 3139 4054 6218 844
Size: 1181 873 241 915 2164 3489
Fragments arranged by size:
3489 2164 1181 915 873 241
BsrBI CCG'CTC
Cuts at: 1394 1823 3603 5890 6750 1394
Size: 429 1780 2287 860 3507
Fragments arranged by size:
3507 2287 1780 860 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4447 4500 7358 7532 65
Size: 1183 3199 53 2858 174 1396
Fragments arranged by size:
3199 2858 1396 1183 174 53
BsrFI r'CCGG_y
Cuts at: 1700 1810 4801 6035 7507 7694 1700
Size: 110 2991 1234 1472 187 2869
Fragments arranged by size:
2991 2869 1472 1234 187 110
BsrGI T'GTAC_A
Cuts at: 96 3522 96
Size: 3426 5437
BssHII G'CGCG_C
Cuts at: 1925 2403 1925
Size: 478 8385
BssSI C'ACGA_G
Cuts at: 6923 7935 6923
Size: 1012 7851
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2350 3475 794
Size: 1556 1125 6182
Fragments arranged by size:
6182 1556 1125
BstBI TT'CG_AA
Cuts at: 7667 7667
Size: 8863
BstEII G'GTnAC_C
Cuts at: 1184 3052 5404 1184
Size: 1868 2352 4643
Fragments arranged by size:
4643 2352 1868
BstXI CCAn_nnnn'nTGG
Cuts at: 4045 4403 5340 4045
Size: 358 937 7568
Fragments arranged by size:
7568 937 358
BstYI r'GATC_y
Cuts at: 1972 2218 2332 2854 2916 4425 5131 6193 6560
Size: 246 114 522 62 1509 706 1062 367
Cuts at: 6560 6945 6962 8414 8500 8512 8858 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1509 1452 1062 706 522 385 367
346 246 114 86 62 17 12
Bsu36I CC'TnA_GG
Cuts at: 4137 4296 5125 4137
Size: 159 829 7875
Fragments arranged by size:
7875 829 159
BtgI C'CryG_G
Cuts at: 513 1065 2575 2653 3240 3986 4857 5101 6030
Size: 552 1510 78 587 746 871 244 929
Cuts at: 6030 513
Size: 3346
Fragments arranged by size:
3346 1510 929 871 746 587 552 244
78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2706 3511 487
Size: 683 56 1480 805 5839
Fragments arranged by size:
5839 1480 805 683 56
BtsI GCAGTG_nn'
Cuts at: 2544 3476 3903 4812 5686 5905 6379 7173 7193
Size: 932 427 909 874 219 474 794 20
Cuts at: 7193 2544
Size: 4214
Fragments arranged by size:
4214 932 909 874 794 474 427 219
20
ClaI AT'CG_AT
Cuts at: 1554 4465 1554
Size: 2911 5952
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2918 2953 3030 3065 5799 5834 529
Size: 35 2354 35 77 35 2734 35 3558
Fragments arranged by size:
3558 2734 2354 77 35 35 35 35
DraI TTT'AAA
Cuts at: 6167 6167
Size: 8863
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2420 7870 809
Size: 507 1104 5450 1802
Fragments arranged by size:
5450 1802 1104 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2061 2316 2408
Size: 54 1425 50 275 18 231 255 92
Cuts at: 2408 2578 3306 3507 3570 4545 5022 5034 5246
Size: 170 728 201 63 975 477 12 212
Cuts at: 5246 6033 6088 6310 7199 8828 8
Size: 787 55 222 889 1629 43
Fragments arranged by size:
1629 1425 975 889 787 728 477 275
255 231 222 212 201 170 92 63
55 54 50 43 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 2408 5034 6033 6310 1537
Size: 275 596 2626 999 277 4090
Fragments arranged by size:
4090 2626 999 596 277 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2378 2625 5775 5829 5958 6052 6793 1353
Size: 1025 247 3150 54 129 94 741 3423
Fragments arranged by size:
3423 3150 1025 741 247 129 94 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3002 4025 7450 7824 7970 387
Size: 1064 138 1413 1023 3425 374 146 1280
Fragments arranged by size:
3425 1413 1280 1064 1023 374 146 138
EcoICRI GAG'CTC
Cuts at: 4768 4768
Size: 8863
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2065 2286 2623 2775 2789 3030 3105
Size: 393 433 221 337 152 14 241 75
Cuts at: 3105 3173 3341 3350 3476 3769 3857 4194 4634
Size: 68 168 9 126 293 88 337 440
Cuts at: 4634 4646 4857 5145 5164 5336 5371 5906 6043
Size: 12 211 288 19 172 35 535 137
Cuts at: 6043 6926 7523 8310 8578 1239
Size: 883 597 787 268 1524
Fragments arranged by size:
1524 883 787 597 535 440 433 393
337 337 293 288 268 241 221 211
172 168 152 137 126 88 75 68
35 19 14 12 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2840 3308 4140 5779 2840
Size: 468 832 1639 5924
Fragments arranged by size:
5924 1639 832 468
EcoO109I rG'GnC_Cy
Cuts at: 3163 3313 3910 3982 4049 4133 4215 4303 4828
Size: 150 597 72 67 84 82 88 525
Cuts at: 4828 5051 5138 5735 5848 6082 6083 3163
Size: 223 87 597 113 234 1 5943
Fragments arranged by size:
5943 597 597 525 234 223 150 113
88 87 84 82 72 67 1
EcoRI G'AATT_C
Cuts at: 6172 6550 6172
Size: 378 8485
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5225 5225
Size: 8863
FspI TGC'GCA
Cuts at: 2343 2789 7369 2343
Size: 446 4580 3837
Fragments arranged by size:
4580 3837 446
FspAI rTGC'GCAy
Cuts at: 2343 2343
Size: 8863
HaeII r_GCGC'y
Cuts at: 1678 2123 2370 2401 3205 4715 4750 8010 1678
Size: 445 247 31 804 1510 35 3260 2531
Fragments arranged by size:
3260 2531 1510 804 445 247 35 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3064 3096 4154 4186 5221
Size: 32 135 32 1492 32 1058 32 1035
Cuts at: 5221 5253 6085 6091 6117 6123 7504 7536 7578
Size: 32 832 6 26 6 1381 32 42
Cuts at: 7578 7610 1373
Size: 32 2626
Fragments arranged by size:
2626 1492 1381 1058 1035 832 135 42
32 32 32 32 32 32 32 26
6 6
HincII GTy'rAC
Cuts at: 669 1657 1938 6207 6453 669
Size: 988 281 4269 246 3079
Fragments arranged by size:
4269 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 5207 5992 748
Size: 4459 785 3619
Fragments arranged by size:
4459 3619 785
HpaI GTT'AAC
Cuts at: 6453 6453
Size: 8863
KasI G'GCGC_C
Cuts at: 2366 2397 4746 2366
Size: 31 2349 6483
Fragments arranged by size:
6483 2349 31
KpnI G_GTAC'C
Cuts at: 3519 6188 3519
Size: 2669 6194
MfeI C'AATT_G
Cuts at: 6462 6462
Size: 8863
MluI A'CGCG_T
Cuts at: 8739 8739
Size: 8863
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2100 2745 4140 6083 7977 8161 1305
Size: 795 645 1395 1943 1894 184 2007
Fragments arranged by size:
2007 1943 1894 1395 795 645 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2318 3308 3509 3572 5248
Size: 54 1425 343 486 990 201 63 1676
Cuts at: 5248 10
Size: 3625
Fragments arranged by size:
3625 1676 1425 990 486 343 201 63
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2432 2705 2952 2981 3242 5958 6821
Size: 648 1266 273 247 29 261 2716 863
Cuts at: 6821 7180 7339 518
Size: 359 159 2042
Fragments arranged by size:
2716 2042 1266 863 648 359 273 261
247 159 29
MspA1I CmG'CkG
Cuts at: 1606 2364 2577 2753 3242 3564 3797 3835 3855
Size: 758 213 176 489 322 233 38 20
Cuts at: 3855 4096 4594 4695 5078 5944 6279 6956 8104
Size: 241 498 101 383 866 335 677 1148
Cuts at: 8104 8349 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 866 758 677 498 489 383
335 322 245 241 233 213 176 101
38 20
NaeI GCC'GGC
Cuts at: 6037 6037
Size: 8863
NarI GG'CG_CC
Cuts at: 2367 2398 4747 2367
Size: 31 2349 6483
Fragments arranged by size:
6483 2349 31
NcoI C'CATG_G
Cuts at: 513 1065 3986 4857 5101 513
Size: 552 2921 871 244 4275
Fragments arranged by size:
4275 2921 871 552 244
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8863
NgoMIV G'CCGG_C
Cuts at: 6035 6035
Size: 8863
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8863
NotI GC'GGCC_GC
Cuts at: 6310 6310
Size: 8863
NspI r_CATG'y
Cuts at: 1132 1229 2704 2951 5515 5878 6027 6221 7766
Size: 97 1475 247 2564 363 149 194 1545
Cuts at: 7766 1132
Size: 2229
Fragments arranged by size:
2564 2229 1545 1475 363 247 194 149
97
PasI CC'CwG_GG
Cuts at: 1348 4938 1348
Size: 3590 5273
PciI A'CATG_T
Cuts at: 6023 7762 6023
Size: 1739 7124
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3141 3749 3797 1108
Size: 2033 608 48 6174
Fragments arranged by size:
6174 2033 608 48
PfoI T'CCnGG_A
Cuts at: 2518 2600 3082 4070 2518
Size: 82 482 988 7311
Fragments arranged by size:
7311 988 482 82
PmeI GTTT'AAAC
Cuts at: 6167 6167
Size: 8863
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4371 4403 6932 6964 8445 8477 4371
Size: 32 2529 32 1481 32 4757
Fragments arranged by size:
4757 2529 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3163 3982 4049 4215 5051 5138 5735 3163
Size: 819 67 166 836 87 597 6291
Fragments arranged by size:
6291 836 819 597 166 87 67
PshAI GACnn'nnGTC
Cuts at: 1613 5455 1613
Size: 3842 5021
PsiI TTA'TAA
Cuts at: 6433 6433
Size: 8863
PspOMI G'GGCC_C
Cuts at: 5847 6082 5847
Size: 235 8628
PstI C_TGCA'G
Cuts at: 830 2037 3422 3480 3760 3864 4032 4065 4474
Size: 1207 1385 58 280 104 168 33 409
Cuts at: 4474 5094 5635 6215 830
Size: 620 541 580 3478
Fragments arranged by size:
3478 1385 1207 620 580 541 409 280
168 104 58 33
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8863
PvuII CAG'CTG
Cuts at: 2753 3564 3797 3835 4096 4594 2753
Size: 811 233 38 261 498 7022
Fragments arranged by size:
7022 811 498 261 233 38
SacI G_AGCT'C
Cuts at: 4770 4770
Size: 8863
SacII CC_GC'GG
Cuts at: 2578 3243 2578
Size: 665 8198
SalI G'TCGA_C
Cuts at: 1936 6205 1936
Size: 4269 4594
SanDI GG'GwC_CC
Cuts at: 4049 4215 5138 4049
Size: 166 923 7774
Fragments arranged by size:
7774 923 166
SapI GCTCTTCn'nnn_
Cuts at: 5775 5775
Size: 8863
SbfI CC_TGCA'GG
Cuts at: 4032 4065 6215 4032
Size: 33 2150 6680
Fragments arranged by size:
6680 2150 33
ScaI AGT'ACT
Cuts at: 2556 4189 7111 8823 2556
Size: 1633 2922 1712 2596
Fragments arranged by size:
2922 2596 1712 1633
SfcI C'TryA_G
Cuts at: 826 1045 2033 3418 3476 3559 3651 3756 3860
Size: 219 988 1385 58 83 92 105 104
Cuts at: 3860 4028 4061 4166 4470 5059 5090 5631 5776
Size: 168 33 105 304 589 31 541 145
Cuts at: 5776 6211 7346 8027 8218 826
Size: 435 1135 681 191 1471
Fragments arranged by size:
1471 1385 1135 988 681 589 541 435
304 219 191 168 145 105 105 104
92 83 58 33 31
SfoI GGC'GCC
Cuts at: 2368 2399 4748 2368
Size: 31 2349 6483
Fragments arranged by size:
6483 2349 31
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8863
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8863
SmaI CCC'GGG
Cuts at: 1872 4621 6005 6080 6190 1872
Size: 2749 1384 75 110 4545
Fragments arranged by size:
4545 2749 1384 110 75
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2624 2684 3611 6967 7868 8130
Size: 197 926 681 60 927 3356 901 262
Cuts at: 8130 820
Size: 1553
Fragments arranged by size:
3356 1553 927 926 901 681 262 197
60
SnaBI TAC'GTA
Cuts at: 493 6654 493
Size: 6161 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8863
SphI G_CATG'C
Cuts at: 1132 6221 1132
Size: 5089 3774
SrfI GCCC'GGGC
Cuts at: 4621 4621
Size: 8863
SspI AAT'ATT
Cuts at: 5 52 6787 5
Size: 47 6735 2081
Fragments arranged by size:
6735 2081 47
StuI AGG'CCT
Cuts at: 1517 3782 5608 5969 1517
Size: 2265 1826 361 4411
Fragments arranged by size:
4411 2265 1826 361
StyI C'CwwG_G
Cuts at: 513 1065 3309 3986 4243 4338 4857 4864 5101
Size: 552 2244 677 257 95 519 7 237
Cuts at: 5101 5190 5548 5763 6302 513
Size: 89 358 215 539 3074
Fragments arranged by size:
3074 2244 677 552 539 519 358 257
237 215 95 89 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4870 4923 5609 5970 6890 6907 7060
Size: 1255 3290 53 686 361 920 17 153
Cuts at: 7060 7245 7690 325
Size: 185 445 1498
Fragments arranged by size:
3290 1498 1255 920 686 445 361 185
153 53 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2554 3522 4187
Size: 274 80 33 51 1443 577 968 665
Cuts at: 4187 7109 8821 96
Size: 2922 1712 138
Fragments arranged by size:
2922 1712 1443 968 665 577 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2514 2546 2599 2631 3078 3110 3455 3487 4317
Size: 32 53 32 447 32 345 32 830
Cuts at: 4317 4349 5187 5219 8823 8855 2514
Size: 32 838 32 3604 32 2522
Fragments arranged by size:
3604 2522 838 830 447 345 53 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8863
XbaI T'CTAG_A
Cuts at: 6199 6199
Size: 8863
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3316 3385 4558 4855 5180 5694 3316
Size: 69 1173 297 325 514 6485
Fragments arranged by size:
6485 1173 514 325 297 69
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8863
XmaI C'CCGG_G
Cuts at: 1870 4619 6003 6078 6188 1870
Size: 2749 1384 75 110 4545
Fragments arranged by size:
4545 2749 1384 110 75
XmnI GAAnn'nnTTC
Cuts at: 2045 6992 2045
Size: 4947 3916
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6671 276
Size: 53 83 186 936 5137 2468
Fragments arranged by size:
5137 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SacII SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AscI AvrII BfrBI BsiWI BstZ17I DraIII EcoRV
FseI NruI NsiI PacI PmlI PspXI PsrI RsrII
SexAI SfiI SwaI