Restriction map for
pFN21AE6726
(Circular) (Six-base) MAPSORT of: pFN21AE6726.seq Check: 423 from: 1 to: 6799
pFN21AE6726 6799 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4609 278
Size: 53 83 186 936 3073 2468
Fragments arranged by size:
3073 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2826 4142 1937
Size: 889 1316 4594
Fragments arranged by size:
4594 1316 889
Acc65I G'GTAC_C
Cuts at: 3171 4120 3171
Size: 949 5850
AclI AA'CG_TT
Cuts at: 1563 4926 5299 1563
Size: 3363 373 3063
Fragments arranged by size:
3363 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2217 2348 2531 2558 2678 2880 3347 4862
Size: 585 131 183 27 120 202 467 1515
Cuts at: 4862 6246 6514 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1515 1384 585 467 268 202 183
131 120 27
AflII C'TTAA_G
Cuts at: 820 1017 2819 820
Size: 197 1802 4800
Fragments arranged by size:
4800 1802 197
AflIII A'CryG_T
Cuts at: 1203 5698 6675 1203
Size: 4495 977 1327
Fragments arranged by size:
4495 1327 977
AgeI A'CCGG_T
Cuts at: 5630 5630
Size: 6799
AhdI GACnn_n'nnGTC
Cuts at: 5528 5528
Size: 6799
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6799
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2808 1184
Size: 1624 5175
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3357 3389 3357
Size: 32 6767
AlwNI CAG_nnn'CTG
Cuts at: 2788 3000 3181 3421 4542 6114 2788
Size: 212 181 240 1121 1572 3473
Fragments arranged by size:
3473 1572 1121 240 212 181
ApaI G_GGCC'C
Cuts at: 2746 3113 2746
Size: 367 6432
ApaLI G'TGCA_C
Cuts at: 4856 6012 4856
Size: 1156 5643
ApoI r'AATT_y
Cuts at: 1493 2552 3452 4108 4334 4486 6595 1493
Size: 1059 900 656 226 152 2109 1697
Fragments arranged by size:
2109 1697 1059 900 656 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5353 160
Size: 872 4321 1606
Fragments arranged by size:
4321 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6799
AvaI C'yCGr_G
Cuts at: 1870 1943 2847 4124 1870
Size: 73 904 1277 4545
Fragments arranged by size:
4545 1277 904 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2351 2384 2351
Size: 33 6766
BamHI G'GATC_C
Cuts at: 4129 4496 4129
Size: 367 6432
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3171 4120 5575 618
Size: 325 217 627 1384 949 1455 1842
Fragments arranged by size:
1842 1455 1384 949 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2588 2746 2947 3093 3113 3540 3843 4004
Size: 1197 158 201 146 20 427 303 161
Cuts at: 4004 4118 1391
Size: 114 4072
Fragments arranged by size:
4072 1197 427 303 201 161 158 146
114 20
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2397 3012 3138 3645 928
Size: 971 498 615 126 507 4082
Fragments arranged by size:
4082 971 615 507 498 126
BbvCI CC'TCA_GC
Cuts at: 2931 2931
Size: 6799
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2816 2850 4990 5024 1903
Size: 34 50 34 795 34 2140 34 3678
Fragments arranged by size:
3678 2140 795 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4691 5901 4691
Size: 1210 5589
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6799
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5410 136
Size: 107 122 71 631 605 3738 1525
Fragments arranged by size:
3738 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3333 3825 6794 3333
Size: 492 2969 3338
Fragments arranged by size:
3338 2969 492
BlpI GC'TnA_GC
Cuts at: 2625 2777 2937 4216 2625
Size: 152 160 1279 5208
Fragments arranged by size:
5208 1279 160 152
Bme1580I G_kGCm'C
Cuts at: 1790 2746 3113 4860 6016 1790
Size: 956 367 1747 1156 2573
Fragments arranged by size:
2573 1747 1156 956 367
BmgBI CAC'GTC
Cuts at: 1318 2170 1318
Size: 852 5947
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3284 4530 5488
Size: 416 53 83 399 24 1867 1246 958
Cuts at: 5488 442
Size: 1753
Fragments arranged by size:
1867 1753 1246 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6799
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2602 2602
Size: 6799
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2572 3122 3254 3902 5459 1239
Size: 1333 550 132 648 1557 2579
Fragments arranged by size:
2579 1557 1333 648 550 132
Bpu10I CC'TnA_GC
Cuts at: 2931 3988 2931
Size: 1057 5742
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4001 4924 5789 6087 4001
Size: 923 865 298 4713
Fragments arranged by size:
4713 923 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2024 5462 882
Size: 617 525 3438 2219
Fragments arranged by size:
3438 2219 617 525
BsaAI yAC'GTr
Cuts at: 493 1204 4590 493
Size: 711 3386 2702
Fragments arranged by size:
3386 2702 711
BsaBI GATnn'nnATC
Cuts at: 6793 6793
Size: 6799
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4606 4988 5623 275
Size: 53 83 186 936 3073 382 635 1451
Fragments arranged by size:
3073 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3753 5232 5630 5904 6051 6462 3753
Size: 1479 398 274 147 411 4090
Fragments arranged by size:
4090 1479 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3830 3860 1178
Size: 30 165 30 2427 30 4117
Fragments arranged by size:
4117 2427 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2474 2777 3028 3485 3857 1186
Size: 1288 303 251 457 372 4128
Fragments arranged by size:
4128 1288 457 372 303 251
BseYI C'CCAG_C
Cuts at: 2025 2293 2340 3094 3101 6002 6498 2025
Size: 268 47 754 7 2901 496 2326
Fragments arranged by size:
2901 2326 754 496 268 47 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2884 1163
Size: 573 1148 5078
Fragments arranged by size:
5078 1148 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4249 5010 6038 1540
Size: 275 186 2248 761 1028 2301
Fragments arranged by size:
2301 2248 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2169 2588 2631 2947 3540 4860 4945 6016 2169
Size: 419 43 316 593 1320 85 1071 2952
Fragments arranged by size:
2952 1320 1071 593 419 316 85 43
BsmI GAATG_Cn'
Cuts at: 3500 3901 4310 4403 3500
Size: 401 409 93 5896
Fragments arranged by size:
5896 409 401 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3734 3734
Size: 6799
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2169 2588 2631 2746 2947 3093 3113
Size: 399 379 419 43 115 201 146 20
Cuts at: 3113 3540 3843 4004 4118 4860 4945 6016 1391
Size: 427 303 161 114 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 427 419 399 379 303
201 161 146 115 114 85 43 20
BspEI T'CCGG_A
Cuts at: 6462 6462
Size: 6799
BspHI T'CATG_A
Cuts at: 3903 4688 6418 3903
Size: 785 1730 4284
Fragments arranged by size:
4284 1730 785
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4154 844
Size: 3310 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2707 4686 1394
Size: 429 884 1979 3507
Fragments arranged by size:
3507 1979 884 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3309 3507 5294 5468 65
Size: 1183 2061 198 1787 174 1396
Fragments arranged by size:
2061 1787 1396 1183 198 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5443 5630 1700
Size: 110 3633 187 2869
Fragments arranged by size:
3633 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6799
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6799
BssSI C'ACGA_G
Cuts at: 4859 5871 4859
Size: 1012 5787
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2282 794
Size: 1488 5311
BstBI TT'CG_AA
Cuts at: 5603 5603
Size: 6799
BstEII G'GTnAC_C
Cuts at: 1184 3659 1184
Size: 2475 4324
BstYI r'GATC_y
Cuts at: 1972 2730 3333 3345 3528 3825 4129 4496 4881
Size: 758 603 12 183 297 304 367 385
Cuts at: 4881 4898 6350 6436 6448 6794 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 758 603 385 367 346 304
297 183 86 17 12 12
Bsu36I CC'TnA_GG
Cuts at: 2444 2444
Size: 6799
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6247
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6060
Fragments arranged by size:
6060 683 56
BtsI GCAGTG_nn'
Cuts at: 3173 3206 4315 5109 5129 3173
Size: 33 1109 794 20 4843
Fragments arranged by size:
4843 1109 794 33 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6799
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6764
DraI TTT'AAA
Cuts at: 4103 4103
Size: 6799
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4058 5806 809
Size: 507 2742 1748 1802
Fragments arranged by size:
2742 1802 1748 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3758 4246 5135
Size: 54 1425 50 275 18 1928 488 889
Cuts at: 5135 6764 8
Size: 1629 43
Fragments arranged by size:
1928 1629 1425 889 488 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4246 1537
Size: 275 2434 4090
Fragments arranged by size:
4090 2434 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2133 2576 2899 3024 4729 1353
Size: 780 443 323 125 1705 3423
Fragments arranged by size:
3423 1705 780 443 323 125
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2723 5386 5760 5906 387
Size: 1064 138 1134 2663 374 146 1280
Fragments arranged by size:
2663 1280 1134 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2586 2945 3538 2586
Size: 359 593 5847
Fragments arranged by size:
5847 593 359
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2217 2348 2531 2558 2572 2678 2880
Size: 393 585 131 183 27 14 106 202
Cuts at: 2880 3122 3254 3347 3902 4862 5459 6246 6514
Size: 242 132 93 555 960 597 787 268
Cuts at: 6514 1239
Size: 1524
Fragments arranged by size:
1524 960 787 597 585 555 393 268
242 202 183 132 131 106 93 27
14
EcoNI CCTnn'n_nnAGG
Cuts at: 2819 3272 2819
Size: 453 6346
EcoO109I rG'GnC_Cy
Cuts at: 2041 2440 2743 2962 3109 3220 3556 2041
Size: 399 303 219 147 111 336 5284
Fragments arranged by size:
5284 399 336 303 219 147 111
EcoRI G'AATT_C
Cuts at: 2552 4108 4486 2552
Size: 1556 378 4865
Fragments arranged by size:
4865 1556 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2084 2834 3352 4042 2084
Size: 750 518 690 4841
Fragments arranged by size:
4841 750 690 518
FspI TGC'GCA
Cuts at: 5305 5305
Size: 6799
HaeII r_GCGC'y
Cuts at: 1678 3391 3814 4096 5946 1678
Size: 1713 423 282 1850 2531
Fragments arranged by size:
2531 1850 1713 423 282
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2234 2264 2266 2296 2483
Size: 32 135 32 662 30 2 30 187
Cuts at: 2483 2515 2893 2925 3712 3744 3772 3804 3830
Size: 32 378 32 787 32 28 32 26
Cuts at: 3830 3862 5440 5472 5514 5546 1373
Size: 32 1578 32 42 32 2626
Fragments arranged by size:
2626 1578 787 662 378 187 135 42
32 32 32 32 32 32 32 32
32 30 30 28 26 2
HincII GTy'rAC
Cuts at: 669 1657 1938 2827 3210 4143 4389 669
Size: 988 281 889 383 933 246 3079
Fragments arranged by size:
3079 988 933 889 383 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6799
HpaI GTT'AAC
Cuts at: 4389 4389
Size: 6799
KpnI G_GTAC'C
Cuts at: 3175 4124 3175
Size: 949 5850
MfeI C'AATT_G
Cuts at: 4398 4398
Size: 6799
MluI A'CGCG_T
Cuts at: 6675 6675
Size: 6799
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2079 5913 6097 1305
Size: 774 3834 184 2007
Fragments arranged by size:
3834 2007 774 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3760 10
Size: 54 1425 343 1928 3049
Fragments arranged by size:
3049 1928 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3161 3290 3789 4757 5116 5275 518
Size: 648 1995 129 499 968 359 159 2042
Fragments arranged by size:
2042 1995 968 648 499 359 159 129
MspA1I CmG'CkG
Cuts at: 1606 2788 2860 3181 4215 4892 6040 6285 1606
Size: 1182 72 321 1034 677 1148 245 2120
Fragments arranged by size:
2120 1182 1148 1034 677 321 245 72
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6247
NdeI CA'TA_TG
Cuts at: 387 2181 3763 387
Size: 1794 1582 3423
Fragments arranged by size:
3423 1794 1582
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6799
NotI GC'GGCC_GC
Cuts at: 4246 4246
Size: 6799
NspI r_CATG'y
Cuts at: 1132 1229 3715 4157 5702 1132
Size: 97 2486 442 1545 2229
Fragments arranged by size:
2486 2229 1545 442 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6799
PciI A'CATG_T
Cuts at: 5698 5698
Size: 6799
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2788 1108
Size: 1680 5119
PfoI T'CCnGG_A
Cuts at: 2556 3524 3879 2556
Size: 968 355 5476
Fragments arranged by size:
5476 968 355
PmeI GTTT'AAAC
Cuts at: 4103 4103
Size: 6799
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4868 4900 6381 6413 4868
Size: 32 1481 32 5254
Fragments arranged by size:
5254 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2962 3556 2962
Size: 594 6205
PshAI GACnn'nnGTC
Cuts at: 1613 2061 1613
Size: 448 6351
PsiI TTA'TAA
Cuts at: 4369 4369
Size: 6799
PspOMI G'GGCC_C
Cuts at: 2742 3109 2742
Size: 367 6432
PstI C_TGCA'G
Cuts at: 830 2760 2775 3186 4151 830
Size: 1930 15 411 965 3478
Fragments arranged by size:
3478 1930 965 411 15
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6799
PvuII CAG'CTG
Cuts at: 2788 2860 3181 2788
Size: 72 321 6406
Fragments arranged by size:
6406 321 72
SacI G_AGCT'C
Cuts at: 2588 2947 3540 2588
Size: 359 593 5847
Fragments arranged by size:
5847 593 359
SalI G'TCGA_C
Cuts at: 1936 2825 4141 1936
Size: 889 1316 4594
Fragments arranged by size:
4594 1316 889
SapI GCTCTTCn'nnn_
Cuts at: 2576 2576
Size: 6799
SbfI CC_TGCA'GG
Cuts at: 4151 4151
Size: 6799
ScaI AGT'ACT
Cuts at: 5047 6759 5047
Size: 1712 5087
SexAI A'CCwGG_T
Cuts at: 3915 3915
Size: 6799
SfcI C'TryA_G
Cuts at: 826 1045 2756 2771 3182 4147 5282 5963 6154
Size: 219 1711 15 411 965 1135 681 191
Cuts at: 6154 826
Size: 1471
Fragments arranged by size:
1711 1471 1135 965 681 411 219 191
15
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6799
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6799
SmaI CCC'GGG
Cuts at: 1872 4126 1872
Size: 2254 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2819 4016 4903 5804 6066 820
Size: 197 926 876 1197 887 901 262 1553
Fragments arranged by size:
1553 1197 926 901 887 876 262 197
SnaBI TAC'GTA
Cuts at: 493 4590 493
Size: 4097 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6799
SphI G_CATG'C
Cuts at: 1132 4157 1132
Size: 3025 3774
SspI AAT'ATT
Cuts at: 5 52 4723 5
Size: 47 4671 2081
Fragments arranged by size:
4671 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6799
StyI C'CwwG_G
Cuts at: 513 1065 2322 2598 4065 4238 513
Size: 552 1257 276 1467 173 3074
Fragments arranged by size:
3074 1467 1257 552 276 173
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2146 4826 4843 4996 5181 5626 325
Size: 1255 566 2680 17 153 185 445 1498
Fragments arranged by size:
2680 1498 1255 566 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5045 6757 96
Size: 274 80 33 51 1443 3068 1712 138
Fragments arranged by size:
3068 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6759 6791 6759
Size: 32 6767
Tth111I GACn'n_nGTC
Cuts at: 1316 2396 1316
Size: 1080 5719
XbaI T'CTAG_A
Cuts at: 4135 4135
Size: 6799
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2596 3514 2596
Size: 918 5881
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6799
XmaI C'CCGG_G
Cuts at: 1870 4124 1870
Size: 2254 4545
XmnI GAAnn'nnTTC
Cuts at: 4928 4928
Size: 6799
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4607 276
Size: 53 83 186 936 3073 2468
Fragments arranged by size:
3073 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BbeI BfrBI BsiWI BstXI
BstZ17I DraIII EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII
SacII SanDI SfiI SfoI SrfI SwaI