Restriction map for
pFN21AE6724
(Circular) (Six-base) MAPSORT of: pFN21AE6724.seq Check: 8171 from: 1 to: 6784
pFN21AE6724 6784 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4594 278
Size: 53 83 186 936 3058 2468
Fragments arranged by size:
3058 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2475 2745 3949 4127 1937
Size: 538 270 1204 178 4594
Fragments arranged by size:
4594 1204 538 270 178
Acc65I G'GTAC_C
Cuts at: 4105 4105
Size: 6784
AclI AA'CG_TT
Cuts at: 1563 2981 3672 4911 5284 1563
Size: 1418 691 1239 373 3063
Fragments arranged by size:
3063 1418 1239 691 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2274 2417 2598 2633 3024 3375 4847 6231
Size: 642 143 181 35 391 351 1472 1384
Cuts at: 6231 6499 1632
Size: 268 1917
Fragments arranged by size:
1917 1472 1384 642 391 351 268 181
143 35
AflII C'TTAA_G
Cuts at: 820 1017 2054 820
Size: 197 1037 5550
Fragments arranged by size:
5550 1037 197
AflIII A'CryG_T
Cuts at: 1203 2411 3588 5683 6660 1203
Size: 1208 1177 2095 977 1327
Fragments arranged by size:
2095 1327 1208 1177 977
AgeI A'CCGG_T
Cuts at: 5615 5615
Size: 6784
AhdI GACnn_n'nnGTC
Cuts at: 2997 5513 2997
Size: 2516 4268
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6784
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6784
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2193 2225 2193
Size: 32 6752
AlwNI CAG_nnn'CTG
Cuts at: 3326 4527 6099 3326
Size: 1201 1572 4011
Fragments arranged by size:
4011 1572 1201
ApaLI G'TGCA_C
Cuts at: 2357 2807 4841 5997 2357
Size: 450 2034 1156 3144
Fragments arranged by size:
3144 2034 1156 450
ApoI r'AATT_y
Cuts at: 1493 2163 2310 2617 2877 3264 4093 4319 4471
Size: 670 147 307 260 387 829 226 152
Cuts at: 4471 6580 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 829 670 387 307 260 226
152 147
AseI AT'TA_AT
Cuts at: 160 1032 5338 160
Size: 872 4306 1606
Fragments arranged by size:
4306 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6784
AvaI C'yCGr_G
Cuts at: 1870 1943 4109 1870
Size: 73 2166 4545
Fragments arranged by size:
4545 2166 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3021 3054 3021
Size: 33 6751
BamHI G'GATC_C
Cuts at: 4114 4481 4114
Size: 367 6417
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4105 5560 618
Size: 325 217 627 2318 1455 1842
Fragments arranged by size:
2318 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3195 4103 1391
Size: 1804 908 4072
Fragments arranged by size:
4072 1804 908
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5813
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4975 5009 1903
Size: 34 3038 34 3678
Fragments arranged by size:
3678 3038 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3022 4676 5886 3022
Size: 1654 1210 3920
Fragments arranged by size:
3920 1654 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6784
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5395 136
Size: 107 122 71 631 605 3723 1525
Fragments arranged by size:
3723 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3902 6779 3902
Size: 2877 3907
BlpI GC'TnA_GC
Cuts at: 4201 4201
Size: 6784
Bme1580I G_kGCm'C
Cuts at: 1790 2361 2811 4845 6001 1790
Size: 571 450 2034 1156 2573
Fragments arranged by size:
2573 2034 1156 571 450
BmgBI CAC'GTC
Cuts at: 1318 2712 1318
Size: 1394 5390
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3631 4515 5473
Size: 416 53 83 399 24 2214 884 958
Cuts at: 5473 442
Size: 1753
Fragments arranged by size:
2214 1753 958 884 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6784
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2054 2064 2250 3586 5444 1239
Size: 815 10 186 1336 1858 2579
Fragments arranged by size:
2579 1858 1336 815 186 10
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2696 4909 5774 6072 2696
Size: 2213 865 298 3408
Fragments arranged by size:
3408 2213 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2043 5447 882
Size: 617 544 3404 2219
Fragments arranged by size:
3404 2219 617 544
BsaAI yAC'GTr
Cuts at: 493 1204 3589 4575 493
Size: 711 2385 986 2702
Fragments arranged by size:
2702 2385 986 711
BsaBI GATnn'nnATC
Cuts at: 6778 6778
Size: 6784
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4591 4973 5608 275
Size: 53 83 186 936 3058 382 635 1451
Fragments arranged by size:
3058 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5217 5615 5889 6036 6447 5217
Size: 398 274 147 411 5554
Fragments arranged by size:
5554 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2009 2039 2073 2103 2340
Size: 30 165 30 606 30 34 30 237
Cuts at: 2340 2370 2457 2487 3797 3827 1178
Size: 30 87 30 1310 30 4135
Fragments arranged by size:
4135 1310 606 237 165 87 34 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2061 3098 3227 1186
Size: 875 1037 129 4743
Fragments arranged by size:
4743 1037 875 129
BseYI C'CCAG_C
Cuts at: 3818 5987 6483 3818
Size: 2169 496 4119
Fragments arranged by size:
4119 2169 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2174 2681 3454 3581 1163
Size: 573 438 507 773 127 4366
Fragments arranged by size:
4366 773 573 507 438 127
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4234 4995 6023 1540
Size: 275 186 2233 761 1028 2301
Fragments arranged by size:
2301 2233 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2110 2361 2566 2811 3195 3759 4845 4930 6001
Size: 251 205 245 384 564 1086 85 1071
Cuts at: 6001 2110
Size: 2893
Fragments arranged by size:
2893 1086 1071 564 384 251 245 205
85
BsmI GAATG_Cn'
Cuts at: 2044 3517 3532 4295 4388 2044
Size: 1473 15 763 93 4440
Fragments arranged by size:
4440 1473 763 93 15
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2110 2361 2566 2811 3195 3759 4103
Size: 399 320 251 205 245 384 564 344
Cuts at: 4103 4845 4930 6001 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 564 399 384 344 320
251 245 205 85
BspEI T'CCGG_A
Cuts at: 6447 6447
Size: 6784
BspHI T'CATG_A
Cuts at: 3939 4673 6403 3939
Size: 734 1730 4320
Fragments arranged by size:
4320 1730 734
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3961 4139 844
Size: 3117 178 3489
Fragments arranged by size:
3489 3117 178
BsrBI CCG'CTC
Cuts at: 1394 1823 4671 1394
Size: 429 2848 3507
Fragments arranged by size:
3507 2848 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2799 3973 5279 5453 65
Size: 1183 1551 1174 1306 174 1396
Fragments arranged by size:
1551 1396 1306 1183 1174 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3838 5428 5615 1700
Size: 110 2028 1590 187 2869
Fragments arranged by size:
2869 2028 1590 187 110
BsrGI T'GTAC_A
Cuts at: 96 2759 96
Size: 2663 4121
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6784
BssSI C'ACGA_G
Cuts at: 4844 5856 4844
Size: 1012 5772
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6784
BstBI TT'CG_AA
Cuts at: 3916 5588 3916
Size: 1672 5112
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6784
BstYI r'GATC_y
Cuts at: 1972 3902 3972 4114 4481 4866 4883 6335 6421
Size: 1930 70 142 367 385 17 1452 86
Cuts at: 6421 6433 6779 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1930 1452 385 367 346 142 86
70 17 12
BstZ17I GTA'TAC
Cuts at: 3950 3950
Size: 6784
BtgI C'CryG_G
Cuts at: 513 1065 2004 2960 513
Size: 552 939 956 4337
Fragments arranged by size:
4337 956 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6045
Fragments arranged by size:
6045 683 56
BtsI GCAGTG_nn'
Cuts at: 2650 4300 5094 5114 2650
Size: 1650 794 20 4320
Fragments arranged by size:
4320 1650 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6784
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6749
DraI TTT'AAA
Cuts at: 4088 4088
Size: 6784
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5791 809
Size: 507 4475 1802
Fragments arranged by size:
4475 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2062 4231 5120
Size: 54 1425 50 275 18 232 2169 889
Cuts at: 5120 6749 8
Size: 1629 43
Fragments arranged by size:
2169 1629 1425 889 275 232 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4231 1537
Size: 275 2419 4090
Fragments arranged by size:
4090 2419 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2128 2850 3000 3288 3474 4714 1353
Size: 775 722 150 288 186 1240 3423
Fragments arranged by size:
3423 1240 775 722 288 186 150
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5371 5745 5891 387
Size: 1064 138 3782 374 146 1280
Fragments arranged by size:
3782 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3193 3193
Size: 6784
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2054 2064 2250 2274 2417 2598 2633
Size: 393 422 10 186 24 143 181 35
Cuts at: 2633 3024 3375 3586 4847 5444 6231 6499 1239
Size: 391 351 211 1261 597 787 268 1524
Fragments arranged by size:
1524 1261 787 597 422 393 391 351
268 211 186 181 143 35 24 10
EcoNI CCTnn'n_nnAGG
Cuts at: 2435 2435
Size: 6784
EcoO109I rG'GnC_Cy
Cuts at: 2234 2428 2860 3323 3871 2234
Size: 194 432 463 548 5147
Fragments arranged by size:
5147 548 463 432 194
EcoRI G'AATT_C
Cuts at: 2877 4093 4471 2877
Size: 1216 378 5190
Fragments arranged by size:
5190 1216 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2603 2603
Size: 6784
FspI TGC'GCA
Cuts at: 5290 5290
Size: 6784
HaeII r_GCGC'y
Cuts at: 1678 5931 1678
Size: 4253 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2073 2105 2983 3015 3611
Size: 32 135 32 501 32 878 32 596
Cuts at: 3611 3643 4037 4069 5425 5457 5499 5531 1373
Size: 32 394 32 1356 32 42 32 2626
Fragments arranged by size:
2626 1356 878 596 501 394 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3676 4128 4374 669
Size: 988 281 1738 452 246 3079
Fragments arranged by size:
3079 1738 988 452 281 246
HindIII A'AGCT_T
Cuts at: 748 2870 748
Size: 2122 4662
HpaI GTT'AAC
Cuts at: 3676 4374 3676
Size: 698 6086
KpnI G_GTAC'C
Cuts at: 4109 4109
Size: 6784
MfeI C'AATT_G
Cuts at: 4383 4383
Size: 6784
MluI A'CGCG_T
Cuts at: 6660 6660
Size: 6784
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5898 6082 1305
Size: 4593 184 2007
Fragments arranged by size:
4593 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2064 10
Size: 54 1425 343 232 4730
Fragments arranged by size:
4730 1425 343 232 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2073 2399 2804 3180 3252 4742 5101
Size: 648 907 326 405 376 72 1490 359
Cuts at: 5101 5260 518
Size: 159 2042
Fragments arranged by size:
2042 1490 907 648 405 376 359 326
159 72
MspA1I CmG'CkG
Cuts at: 1606 4200 4877 6025 6270 1606
Size: 2594 677 1148 245 2120
Fragments arranged by size:
2594 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5293
Fragments arranged by size:
5293 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6784
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6784
NotI GC'GGCC_GC
Cuts at: 4231 4231
Size: 6784
NspI r_CATG'y
Cuts at: 1132 1229 2415 3664 4142 5687 1132
Size: 97 1186 1249 478 1545 2229
Fragments arranged by size:
2229 1545 1249 1186 478 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6784
PciI A'CATG_T
Cuts at: 2411 5683 2411
Size: 3272 3512
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3326 1108
Size: 2218 4566
PmeI GTTT'AAAC
Cuts at: 4088 4088
Size: 6784
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4853 4885 6366 6398 4853
Size: 32 1481 32 5239
Fragments arranged by size:
5239 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2234 3323 3871 2234
Size: 1089 548 5147
Fragments arranged by size:
5147 1089 548
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6784
PsiI TTA'TAA
Cuts at: 4354 4354
Size: 6784
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3910 3942 3910
Size: 32 6752
PstI C_TGCA'G
Cuts at: 830 4136 830
Size: 3306 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6784
SacI G_AGCT'C
Cuts at: 3195 3195
Size: 6784
SalI G'TCGA_C
Cuts at: 1936 4126 1936
Size: 2190 4594
SanDI GG'GwC_CC
Cuts at: 3871 3871
Size: 6784
SbfI CC_TGCA'GG
Cuts at: 4136 4136
Size: 6784
ScaI AGT'ACT
Cuts at: 3763 5032 6744 3763
Size: 1269 1712 3803
Fragments arranged by size:
3803 1712 1269
SfcI C'TryA_G
Cuts at: 826 1045 3552 3580 3720 3978 4132 5267 5948
Size: 219 2507 28 140 258 154 1135 681
Cuts at: 5948 6139 826
Size: 191 1471
Fragments arranged by size:
2507 1471 1135 681 258 219 191 154
140 28
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6784
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6784
SmaI CCC'GGG
Cuts at: 1872 4111 1872
Size: 2239 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2054 2675 4888 5789 6051 820
Size: 197 926 111 621 2213 901 262 1553
Fragments arranged by size:
2213 1553 926 901 621 262 197 111
SnaBI TAC'GTA
Cuts at: 493 4575 493
Size: 4082 2702
SpeI A'CTAG_T
Cuts at: 152 3929 152
Size: 3777 3007
SphI G_CATG'C
Cuts at: 1132 4142 1132
Size: 3010 3774
SspI AAT'ATT
Cuts at: 5 52 2283 3397 4708 5
Size: 47 2231 1114 1311 2081
Fragments arranged by size:
2231 2081 1311 1114 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6784
StyI C'CwwG_G
Cuts at: 513 1065 2004 2172 3100 4223 513
Size: 552 939 168 928 1123 3074
Fragments arranged by size:
3074 1123 939 928 552 168
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2145 4811 4828 4981 5166 5611 325
Size: 1255 565 2666 17 153 185 445 1498
Fragments arranged by size:
2666 1498 1255 565 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2759 3761 5030
Size: 274 80 33 51 1443 782 1002 1269
Cuts at: 5030 6742 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1269 1002 782 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2340 2372 6744 6776 2340
Size: 32 4372 32 2348
Fragments arranged by size:
4372 2348 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6784
XbaI T'CTAG_A
Cuts at: 4120 4120
Size: 6784
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2579 2579
Size: 6784
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6784
XmaI C'CCGG_G
Cuts at: 1870 4109 1870
Size: 2239 4545
XmnI GAAnn'nnTTC
Cuts at: 2618 3530 4913 2618
Size: 912 1383 4489
Fragments arranged by size:
4489 1383 912
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4592 276
Size: 53 83 186 936 3058 2468
Fragments arranged by size:
3058 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PsrI PstI PvuI SacI SalI SanDI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspOMI PspXI PvuII RsrII SacII
SapI SexAI SfiI SfoI SrfI SwaI