Restriction map for
pFN21AE6723
(Circular) (Six-base) MAPSORT of: pFN21AE6723.seq Check: 3015 from: 1 to: 6802
pFN21AE6723 6802 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4612 278
Size: 53 83 186 936 3076 2468
Fragments arranged by size:
3076 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4145 1937
Size: 2208 4594
Acc65I G'GTAC_C
Cuts at: 2954 4123 2954
Size: 1169 5633
AclI AA'CG_TT
Cuts at: 1563 4929 5302 1563
Size: 3366 373 3063
Fragments arranged by size:
3366 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2124 2544 2569 3134 3458 4865 6249 6517
Size: 492 420 25 565 324 1407 1384 268
Cuts at: 6517 1632
Size: 1917
Fragments arranged by size:
1917 1407 1384 565 492 420 324 268
25
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6605
AflIII A'CryG_T
Cuts at: 1203 3138 3534 3624 5701 6678 1203
Size: 1935 396 90 2077 977 1327
Fragments arranged by size:
2077 1935 1327 977 396 90
AgeI A'CCGG_T
Cuts at: 5633 5633
Size: 6802
AhdI GACnn_n'nnGTC
Cuts at: 3640 5531 3640
Size: 1891 4911
AleI CACnn'nnGTG
Cuts at: 1166 3905 1166
Size: 2739 4063
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3116 1184
Size: 1932 4870
AlwNI CAG_nnn'CTG
Cuts at: 2102 3305 4545 6117 2102
Size: 1203 1240 1572 2787
Fragments arranged by size:
2787 1572 1240 1203
ApaI G_GGCC'C
Cuts at: 2134 2134
Size: 6802
ApaLI G'TGCA_C
Cuts at: 4859 6015 4859
Size: 1156 5646
ApoI r'AATT_y
Cuts at: 1493 4111 4337 4489 6598 1493
Size: 2618 226 152 2109 1697
Fragments arranged by size:
2618 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5356 160
Size: 872 4324 1606
Fragments arranged by size:
4324 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6802
AvaI C'yCGr_G
Cuts at: 1870 1943 4127 1870
Size: 73 2184 4545
Fragments arranged by size:
4545 2184 73
BamHI G'GATC_C
Cuts at: 4132 4499 4132
Size: 367 6435
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2954 3775 4123 5578 618
Size: 325 217 627 1167 821 348 1455 1842
Fragments arranged by size:
1842 1455 1167 821 627 348 325 217
BanII G_rGCy'C
Cuts at: 1391 2134 2454 4121 1391
Size: 743 320 1667 4072
Fragments arranged by size:
4072 1667 743 320
BbeI G_GCGC'C
Cuts at: 3779 3779
Size: 6802
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2448 3661 928
Size: 971 549 1213 4069
Fragments arranged by size:
4069 1213 971 549
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4993 5027 1903
Size: 34 3056 34 3678
Fragments arranged by size:
3678 3056 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4694 5904 4694
Size: 1210 5592
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6802
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5413 136
Size: 107 122 71 631 605 3741 1525
Fragments arranged by size:
3741 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2691 3702 6797 2691
Size: 1011 3095 2696
Fragments arranged by size:
3095 2696 1011
BlpI GC'TnA_GC
Cuts at: 3277 4219 3277
Size: 942 5860
Bme1580I G_kGCm'C
Cuts at: 1790 2134 3551 3960 4863 6019 1790
Size: 344 1417 409 903 1156 2573
Fragments arranged by size:
2573 1417 1156 903 409 344
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6802
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2756 4533 5491
Size: 416 53 83 399 24 1339 1777 958
Cuts at: 5491 442
Size: 1753
Fragments arranged by size:
1777 1753 1339 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6802
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2614 2614
Size: 6802
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2189 3574 5462 1239
Size: 950 1385 1888 2579
Fragments arranged by size:
2579 1888 1385 950
Bpu10I CC'TnA_GC
Cuts at: 2544 2544
Size: 6802
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2738 2886 3227 3956 4927 5792 6090 2738
Size: 148 341 729 971 865 298 3450
Fragments arranged by size:
3450 971 865 729 341 298 148
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5465 882
Size: 617 3966 2219
Fragments arranged by size:
3966 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3181 3907 4593 493
Size: 711 1977 726 686 2702
Fragments arranged by size:
2702 1977 726 711 686
BsaBI GATnn'nnATC
Cuts at: 3734 6796 3734
Size: 3062 3740
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3159 3615 3776 4609
Size: 53 83 186 936 1626 456 161 833
Cuts at: 4609 4991 5626 275
Size: 382 635 1451
Fragments arranged by size:
1626 1451 936 833 635 456 382 186
161 83 53
BsaWI w'CCGG_w
Cuts at: 5235 5633 5907 6054 6465 5235
Size: 398 274 147 411 5572
Fragments arranged by size:
5572 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2632 2662 1178
Size: 30 165 30 1229 30 5318
Fragments arranged by size:
5318 1229 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2210 2640 3569 1186
Size: 1024 430 929 4419
Fragments arranged by size:
4419 1024 929 430
BseYI C'CCAG_C
Cuts at: 2501 3122 3258 6005 6501 2501
Size: 621 136 2747 496 2802
Fragments arranged by size:
2802 2747 621 496 136
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3467 3929 4046 1163
Size: 573 1731 462 117 3919
Fragments arranged by size:
3919 1731 573 462 117
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4252 5013 6041 1540
Size: 275 186 2251 761 1028 2301
Fragments arranged by size:
2301 2251 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3578 4863 4948 6019 3578
Size: 1285 85 1071 4361
Fragments arranged by size:
4361 1285 1071 85
BsmI GAATG_Cn'
Cuts at: 2639 4313 4406 2639
Size: 1674 93 5035
Fragments arranged by size:
5035 1674 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2134 2454 3551 3578 3960 4121 4863
Size: 399 344 320 1097 27 382 161 742
Cuts at: 4863 4948 6019 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1097 1071 742 399 382 344 320
161 85 27
BspEI T'CCGG_A
Cuts at: 6465 6465
Size: 6802
BspHI T'CATG_A
Cuts at: 3075 4691 6421 3075
Size: 1616 1730 3456
Fragments arranged by size:
3456 1730 1616
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2381 2615 3304 4157 844
Size: 1537 234 689 853 3489
Fragments arranged by size:
3489 1537 853 689 234
BsrBI CCG'CTC
Cuts at: 1394 1823 2866 2968 4689 1394
Size: 429 1043 102 1721 3507
Fragments arranged by size:
3507 1721 1043 429 102
BsrDI GCAATG_nn'
Cuts at: 65 1248 2895 5297 5471 65
Size: 1183 1647 2402 174 1396
Fragments arranged by size:
2402 1647 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5446 5633 1700
Size: 110 3636 187 2869
Fragments arranged by size:
3636 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6802
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6802
BssSI C'ACGA_G
Cuts at: 3398 4862 5874 3398
Size: 1464 1012 4326
Fragments arranged by size:
4326 1464 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4024 794
Size: 3230 3572
BstBI TT'CG_AA
Cuts at: 5606 5606
Size: 6802
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6802
BstYI r'GATC_y
Cuts at: 1972 2691 3040 3702 4132 4499 4884 4901 6353
Size: 719 349 662 430 367 385 17 1452
Cuts at: 6353 6439 6451 6797 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 719 662 430 385 367 349
346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3468 3468
Size: 6802
BtgI C'CryG_G
Cuts at: 513 1065 3456 3756 3952 513
Size: 552 2391 300 196 3363
Fragments arranged by size:
3363 2391 552 300 196
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2391 3960 487
Size: 683 56 1165 1569 3329
Fragments arranged by size:
3329 1569 1165 683 56
BtsI GCAGTG_nn'
Cuts at: 3009 3057 3501 4318 5112 5132 3009
Size: 48 444 817 794 20 4679
Fragments arranged by size:
4679 817 794 444 48 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6802
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6767
DraI TTT'AAA
Cuts at: 4106 4106
Size: 6802
DraIII CAC_nnn'GTG
Cuts at: 3490 3490
Size: 6802
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5809 809
Size: 507 4493 1802
Fragments arranged by size:
4493 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2787 3228 3882
Size: 54 1425 50 275 18 957 441 654
Cuts at: 3882 4249 5138 6767 8
Size: 367 889 1629 43
Fragments arranged by size:
1629 1425 957 889 654 441 367 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4249 1537
Size: 275 2437 4090
Fragments arranged by size:
4090 2437 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2075 2442 3398 3790 4732 1353
Size: 722 367 956 392 942 3423
Fragments arranged by size:
3423 956 942 722 392 367
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5389 5763 5909 387
Size: 1064 138 3800 374 146 1280
Fragments arranged by size:
3800 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2124 2189 2544 2569 3134 3458 3574
Size: 393 492 65 355 25 565 324 116
Cuts at: 3574 4865 5462 6249 6517 1239
Size: 1291 597 787 268 1524
Fragments arranged by size:
1524 1291 787 597 565 492 393 355
324 268 116 65 25
EcoO109I rG'GnC_Cy
Cuts at: 2595 2595
Size: 6802
EcoRI G'AATT_C
Cuts at: 4111 4489 4111
Size: 378 6424
FspI TGC'GCA
Cuts at: 3992 5308 3992
Size: 1316 5486
HaeII r_GCGC'y
Cuts at: 1678 3001 3779 5949 1678
Size: 1323 778 2170 2531
Fragments arranged by size:
2531 2170 1323 778
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2632 2664 2812 2844 3626
Size: 32 135 32 1060 32 148 32 782
Cuts at: 3626 3658 3734 3766 5443 5475 5517 5549 1373
Size: 32 76 32 1677 32 42 32 2626
Fragments arranged by size:
2626 1677 1060 782 148 135 76 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2175 2875 4146 4392 669
Size: 988 281 237 700 1271 246 3079
Fragments arranged by size:
3079 1271 988 700 281 246 237
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6802
HpaI GTT'AAC
Cuts at: 4392 4392
Size: 6802
KasI G'GCGC_C
Cuts at: 3775 3775
Size: 6802
KpnI G_GTAC'C
Cuts at: 2958 4127 2958
Size: 1169 5633
MfeI C'AATT_G
Cuts at: 4401 4401
Size: 6802
MluI A'CGCG_T
Cuts at: 6678 6678
Size: 6802
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3711 5916 6100 1305
Size: 2406 2205 184 2007
Fragments arranged by size:
2406 2205 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2789 3230 10
Size: 54 1425 343 957 441 3582
Fragments arranged by size:
3582 1425 957 441 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3179 3423 3629 3905 3938 4760 5119
Size: 648 2013 244 206 276 33 822 359
Cuts at: 5119 5278 518
Size: 159 2042
Fragments arranged by size:
2042 2013 822 648 359 276 244 206
159 33
MspA1I CmG'CkG
Cuts at: 1606 2167 2501 2510 3156 3481 4040 4218 4895
Size: 561 334 9 646 325 559 178 677
Cuts at: 4895 6043 6288 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 646 561 559 334 325
245 178 9
NarI GG'CG_CC
Cuts at: 3776 3776
Size: 6802
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6250
NdeI CA'TA_TG
Cuts at: 387 2887 387
Size: 2500 4302
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6802
NotI GC'GGCC_GC
Cuts at: 4249 4249
Size: 6802
NspI r_CATG'y
Cuts at: 1132 1229 3097 3142 3538 3628 4160 5705 1132
Size: 97 1868 45 396 90 532 1545 2229
Fragments arranged by size:
2229 1868 1545 532 396 97 90 45
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6802
PciI A'CATG_T
Cuts at: 3138 3534 3624 5701 3138
Size: 396 90 2077 4239
Fragments arranged by size:
4239 2077 396 90
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2685 3877 1108
Size: 1577 1192 4033
Fragments arranged by size:
4033 1577 1192
PfoI T'CCnGG_A
Cuts at: 2622 3644 2622
Size: 1022 5780
PmeI GTTT'AAAC
Cuts at: 4106 4106
Size: 6802
PmlI CAC'GTG
Cuts at: 3181 3907 3181
Size: 726 6076
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3125 3157 4871 4903 6384 6416 3125
Size: 32 1714 32 1481 32 3511
Fragments arranged by size:
3511 1714 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6802
PsiI TTA'TAA
Cuts at: 4372 4372
Size: 6802
PspOMI G'GGCC_C
Cuts at: 2130 2130
Size: 6802
PstI C_TGCA'G
Cuts at: 830 2500 3275 3683 4154 830
Size: 1670 775 408 471 3478
Fragments arranged by size:
3478 1670 775 471 408
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6802
PvuII CAG'CTG
Cuts at: 2167 2501 2510 3481 4040 2167
Size: 334 9 971 559 4929
Fragments arranged by size:
4929 971 559 334 9
SalI G'TCGA_C
Cuts at: 1936 4144 1936
Size: 2208 4594
SapI GCTCTTCn'nnn_
Cuts at: 2442 2442
Size: 6802
SbfI CC_TGCA'GG
Cuts at: 4154 4154
Size: 6802
ScaI AGT'ACT
Cuts at: 2915 5050 6762 2915
Size: 2135 1712 2955
Fragments arranged by size:
2955 2135 1712
SexAI A'CCwGG_T
Cuts at: 2946 3195 4074 2946
Size: 249 879 5674
Fragments arranged by size:
5674 879 249
SfcI C'TryA_G
Cuts at: 826 1045 2496 3106 3271 3679 4150 5285 5966
Size: 219 1451 610 165 408 471 1135 681
Cuts at: 5966 6157 826
Size: 191 1471
Fragments arranged by size:
1471 1451 1135 681 610 471 408 219
191 165
SfoI GGC'GCC
Cuts at: 3777 3777
Size: 6802
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6802
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6802
SmaI CCC'GGG
Cuts at: 1872 4129 1872
Size: 2257 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2717 2901 3242 3971 4906 5807
Size: 197 926 774 184 341 729 935 901
Cuts at: 5807 6069 820
Size: 262 1553
Fragments arranged by size:
1553 935 926 901 774 729 341 262
197 184
SnaBI TAC'GTA
Cuts at: 493 4593 493
Size: 4100 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6802
SphI G_CATG'C
Cuts at: 1132 3097 4160 1132
Size: 1965 1063 3774
Fragments arranged by size:
3774 1965 1063
SspI AAT'ATT
Cuts at: 5 52 4726 5
Size: 47 4674 2081
Fragments arranged by size:
4674 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6802
StyI C'CwwG_G
Cuts at: 513 1065 2454 2553 3663 4241 513
Size: 552 1389 99 1110 578 3074
Fragments arranged by size:
3074 1389 1110 578 552 99
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2192 2333 3749 3917 3997 4829 4846
Size: 1255 612 141 1416 168 80 832 17
Cuts at: 4846 4999 5184 5629 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1416 1255 832 612 445 185 168
153 141 80 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2913 3033 3135
Size: 274 80 33 51 1443 936 120 102
Cuts at: 3135 5048 6760 96
Size: 1913 1712 138
Fragments arranged by size:
1913 1712 1443 936 274 138 120 102
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3448 3480 6762 6794 3448
Size: 32 3282 32 3456
Fragments arranged by size:
3456 3282 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6802
XbaI T'CTAG_A
Cuts at: 4138 4138
Size: 6802
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3494 3937 3494
Size: 443 6359
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6802
XmaI C'CCGG_G
Cuts at: 1870 4127 1870
Size: 2257 4545
XmnI GAAnn'nnTTC
Cuts at: 4931 4931
Size: 6802
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4610 276
Size: 53 83 186 936 3076 2468
Fragments arranged by size:
3076 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PpuMI
PspXI PsrI RsrII SacI SacII SanDI SfiI SrfI
SwaI