Restriction map for
pFN21AE6718
(Circular) (Six-base) MAPSORT of: pFN21AE6718.seq Check: 5044 from: 1 to: 6751
pFN21AE6718 6751 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2108 3668 2108
Size: 1560 5191
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4561 278
Size: 53 83 186 936 3025 2468
Fragments arranged by size:
3025 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4094 1937
Size: 2157 4594
Acc65I G'GTAC_C
Cuts at: 4072 4072
Size: 6751
AclI AA'CG_TT
Cuts at: 1563 2510 4878 5251 1563
Size: 947 2368 373 3063
Fragments arranged by size:
3063 2368 947 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2953 3507 3752 4814 6198 6466 1632
Size: 1321 554 245 1062 1384 268 1917
Fragments arranged by size:
1917 1384 1321 1062 554 268 245
AfeI AGC'GCT
Cuts at: 2327 2800 2894 2327
Size: 473 94 6184
Fragments arranged by size:
6184 473 94
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6554
AflIII A'CryG_T
Cuts at: 1203 5650 6627 1203
Size: 4447 977 1327
Fragments arranged by size:
4447 1327 977
AgeI A'CCGG_T
Cuts at: 5582 5582
Size: 6751
AhdI GACnn_n'nnGTC
Cuts at: 5480 5480
Size: 6751
AleI CACnn'nnGTG
Cuts at: 1166 3236 1166
Size: 2070 4681
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2630 1184
Size: 1446 5305
AlwNI CAG_nnn'CTG
Cuts at: 2092 2861 3973 4494 6066 2092
Size: 769 1112 521 1572 2777
Fragments arranged by size:
2777 1572 1112 769 521
ApaI G_GGCC'C
Cuts at: 3744 3744
Size: 6751
ApaLI G'TGCA_C
Cuts at: 2680 4808 5964 2680
Size: 2128 1156 3467
Fragments arranged by size:
3467 2128 1156
ApoI r'AATT_y
Cuts at: 1493 2345 2396 2774 3040 3495 4060 4286 4438
Size: 852 51 378 266 455 565 226 152
Cuts at: 4438 6547 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 852 565 455 378 266 226
152 51
AseI AT'TA_AT
Cuts at: 160 1032 5305 160
Size: 872 4273 1606
Fragments arranged by size:
4273 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6751
AvaI C'yCGr_G
Cuts at: 1870 1943 2916 4076 1870
Size: 73 973 1160 4545
Fragments arranged by size:
4545 1160 973 73
BamHI G'GATC_C
Cuts at: 3793 4081 4448 3793
Size: 288 367 6096
Fragments arranged by size:
6096 367 288
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4072 5527 618
Size: 325 217 627 2285 1455 1842
Fragments arranged by size:
2285 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3609 3676 3744 4070 1391
Size: 2218 67 68 326 4072
Fragments arranged by size:
4072 2218 326 68 67
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3495 928
Size: 971 1596 4184
Fragments arranged by size:
4184 1596 971
BbvCI CC'TCA_GC
Cuts at: 2322 2322
Size: 6751
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4942 4976 1903
Size: 34 3005 34 3678
Fragments arranged by size:
3678 3005 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4643 5853 4643
Size: 1210 5541
BclI T'GATC_A
Cuts at: 1548 3555 1548
Size: 2007 4744
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2858 3276 3942
Size: 107 122 71 631 605 1186 418 666
Cuts at: 3942 5362 136
Size: 1420 1525
Fragments arranged by size:
1525 1420 1186 666 631 605 418 122
107 71
BglII A'GATC_T
Cuts at: 2058 2386 2991 3539 6746 2058
Size: 328 605 548 3207 2063
Fragments arranged by size:
3207 2063 605 548 328
BlpI GC'TnA_GC
Cuts at: 4168 4168
Size: 6751
Bme1580I G_kGCm'C
Cuts at: 1790 2684 3744 4812 5968 1790
Size: 894 1060 1068 1156 2573
Fragments arranged by size:
2573 1156 1068 1060 894
BmgBI CAC'GTC
Cuts at: 1318 2679 1318
Size: 1361 5390
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2295 4482 5440
Size: 416 53 83 399 24 878 2187 958
Cuts at: 5440 442
Size: 1753
Fragments arranged by size:
2187 1753 958 878 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6751
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2114 2708 2855 3596 3623 3923 5411 1239
Size: 875 594 147 741 27 300 1488 2579
Fragments arranged by size:
2579 1488 875 741 594 300 147 27
Bpu10I CC'TnA_GC
Cuts at: 2322 3549 3950 2322
Size: 1227 401 5123
Fragments arranged by size:
5123 1227 401
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3556 4876 5741 6039 3556
Size: 1320 865 298 4268
Fragments arranged by size:
4268 1320 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2438 3275 3371 5414 882
Size: 617 939 837 96 2043 2219
Fragments arranged by size:
2219 2043 939 837 617 96
BsaAI yAC'GTr
Cuts at: 493 1204 4542 493
Size: 711 3338 2702
Fragments arranged by size:
3338 2702 711
BsaBI GATnn'nnATC
Cuts at: 2663 6745 2663
Size: 4082 2669
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3342 3390 4558 4940
Size: 53 83 186 936 1809 48 1168 382
Cuts at: 4940 5575 275
Size: 635 1451
Fragments arranged by size:
1809 1451 1168 936 635 382 186 83
53 48
BsaWI w'CCGG_w
Cuts at: 2628 5184 5582 5856 6003 6414 2628
Size: 2556 398 274 147 411 2965
Fragments arranged by size:
2965 2556 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2574 2604 2630 2660 1178
Size: 30 165 30 1171 30 26 30 5269
Fragments arranged by size:
5269 1171 165 30 30 30 30 26
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3593 1186
Size: 2407 4344
BseYI C'CCAG_C
Cuts at: 2280 3722 5954 6450 2280
Size: 1442 2232 496 2581
Fragments arranged by size:
2581 2232 1442 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6178
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2130 4201 4962 5990 1540
Size: 275 186 129 2071 761 1028 2301
Fragments arranged by size:
2301 2071 1028 761 275 186 129
BsiHKAI G_wGCw'C
Cuts at: 2684 2814 3063 3609 3869 4812 4897 5968 2684
Size: 130 249 546 260 943 85 1071 3467
Fragments arranged by size:
3467 1071 943 546 260 249 130 85
BsmI GAATG_Cn'
Cuts at: 2546 4262 4355 2546
Size: 1716 93 4942
Fragments arranged by size:
4942 1716 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2684 2814 3063 3609 3676 3744 3869
Size: 399 894 130 249 546 67 68 125
Cuts at: 3869 4070 4812 4897 5968 1391
Size: 201 742 85 1071 2174
Fragments arranged by size:
2174 1071 894 742 546 399 249 201
130 125 85 68 67
BspEI T'CCGG_A
Cuts at: 2628 6414 2628
Size: 3786 2965
BspHI T'CATG_A
Cuts at: 2955 4640 6370 2955
Size: 1685 1730 3336
Fragments arranged by size:
3336 1730 1685
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2108 3668 4106 844
Size: 1264 1560 438 3489
Fragments arranged by size:
3489 1560 1264 438
BsrBI CCG'CTC
Cuts at: 1394 1823 4638 1394
Size: 429 2815 3507
Fragments arranged by size:
3507 2815 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2265 5246 5420 65
Size: 1183 1017 2981 174 1396
Fragments arranged by size:
2981 1396 1183 1017 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5395 5582 1700
Size: 110 3585 187 2869
Fragments arranged by size:
3585 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6751
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6751
BssSI C'ACGA_G
Cuts at: 4811 5823 4811
Size: 1012 5739
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6751
BstBI TT'CG_AA
Cuts at: 2064 2997 5555 2064
Size: 933 2558 3260
Fragments arranged by size:
3260 2558 933
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6751
BstXI CCAn_nnnn'nTGG
Cuts at: 3355 3664 4027 3355
Size: 309 363 6079
Fragments arranged by size:
6079 363 309
BstYI r'GATC_y
Cuts at: 1972 2058 2386 2658 2991 3539 3585 3793 4081
Size: 86 328 272 333 548 46 208 288
Cuts at: 4081 4448 4833 4850 6302 6388 6400 6746 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 548 385 367 346 333 328
288 272 208 86 86 46 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2290 2769 3150 3285 3322 3336 3354
Size: 552 1225 479 381 135 37 14 18
Cuts at: 3354 3529 513
Size: 175 3735
Fragments arranged by size:
3735 1225 552 479 381 175 135 37
18 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2434 2833 487
Size: 683 56 1208 399 4405
Fragments arranged by size:
4405 1208 683 399 56
BtsI GCAGTG_nn'
Cuts at: 2862 4267 5061 5081 2862
Size: 1405 794 20 4532
Fragments arranged by size:
4532 1405 794 20
ClaI AT'CG_AT
Cuts at: 1554 2130 1554
Size: 576 6175
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6716
DraI TTT'AAA
Cuts at: 4055 4055
Size: 6751
DraIII CAC_nnn'GTG
Cuts at: 3928 3928
Size: 6751
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3389 5758 809
Size: 507 2073 2369 1802
Fragments arranged by size:
2369 2073 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2898 3423 4029
Size: 54 1425 50 275 18 1068 525 606
Cuts at: 4029 4198 5087 6716 8
Size: 169 889 1629 43
Fragments arranged by size:
1629 1425 1068 889 606 525 275 169
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4198 1537
Size: 275 2386 4090
Fragments arranged by size:
4090 2386 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2717 4681 1353
Size: 1364 1964 3423
Fragments arranged by size:
3423 1964 1364
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2728 5338 5712 5858 387
Size: 1064 138 1139 2610 374 146 1280
Fragments arranged by size:
2610 1280 1139 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3607 3607
Size: 6751
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2114 2708 2855 2953 3507 3596 3623
Size: 393 482 594 147 98 554 89 27
Cuts at: 3623 3752 3923 4814 5411 6198 6466 1239
Size: 129 171 891 597 787 268 1524
Fragments arranged by size:
1524 891 787 597 594 554 482 393
268 171 147 129 98 89 27
EcoNI CCTnn'n_nnAGG
Cuts at: 2075 2075
Size: 6751
EcoO109I rG'GnC_Cy
Cuts at: 2080 2296 3741 2080
Size: 216 1445 5090
Fragments arranged by size:
5090 1445 216
EcoRI G'AATT_C
Cuts at: 2774 4060 4438 2774
Size: 1286 378 5087
Fragments arranged by size:
5087 1286 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2077 3010 3100 2077
Size: 933 90 5728
Fragments arranged by size:
5728 933 90
FspI TGC'GCA
Cuts at: 5257 5257
Size: 6751
HaeII r_GCGC'y
Cuts at: 1678 2329 2802 2896 3305 3330 5898 1678
Size: 651 473 94 409 25 2568 2531
Fragments arranged by size:
2568 2531 651 473 409 94 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2411 2443 2668 2700 2897
Size: 32 135 32 839 32 225 32 197
Cuts at: 2897 2929 3099 3131 5392 5424 5466 5498 1373
Size: 32 170 32 2261 32 42 32 2626
Fragments arranged by size:
2626 2261 839 225 197 170 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2311 4012 4095 4341 669
Size: 988 281 373 1701 83 246 3079
Fragments arranged by size:
3079 1701 988 373 281 246 83
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6751
HpaI GTT'AAC
Cuts at: 4341 4341
Size: 6751
KpnI G_GTAC'C
Cuts at: 4076 4076
Size: 6751
MfeI C'AATT_G
Cuts at: 4350 4350
Size: 6751
MluI A'CGCG_T
Cuts at: 6627 6627
Size: 6751
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2517 5865 6049 1305
Size: 1212 3348 184 2007
Fragments arranged by size:
3348 2007 1212 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3425 4031 10
Size: 54 1425 343 1593 606 2730
Fragments arranged by size:
2730 1593 1425 606 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3236 4709 5068 5227 518
Size: 648 2070 1473 359 159 2042
Fragments arranged by size:
2070 2042 1473 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2089 2170 3287 3324 3964 3970 4167 4844
Size: 483 81 1117 37 640 6 197 677
Cuts at: 4844 5992 6237 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 1117 677 640 483 245 197
81 37 6
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6199
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6751
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6751
NotI GC'GGCC_GC
Cuts at: 4198 4198
Size: 6751
NruI TCG'CGA
Cuts at: 3375 3375
Size: 6751
NspI r_CATG'y
Cuts at: 1132 1229 2753 3703 4109 5654 1132
Size: 97 1524 950 406 1545 2229
Fragments arranged by size:
2229 1545 1524 950 406 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6751
PciI A'CATG_T
Cuts at: 5650 5650
Size: 6751
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3595 3928 1108
Size: 2487 333 3931
Fragments arranged by size:
3931 2487 333
PfoI T'CCnGG_A
Cuts at: 2832 2832
Size: 6751
PmeI GTTT'AAAC
Cuts at: 4055 4055
Size: 6751
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4820 4852 6333 6365 4820
Size: 32 1481 32 5206
Fragments arranged by size:
5206 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2296 2296
Size: 6751
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6751
PsiI TTA'TAA
Cuts at: 4321 4321
Size: 6751
PspOMI G'GGCC_C
Cuts at: 3740 3740
Size: 6751
PstI C_TGCA'G
Cuts at: 830 2088 2949 3828 3969 4103 830
Size: 1258 861 879 141 134 3478
Fragments arranged by size:
3478 1258 879 861 141 134
PvuI CG_AT'CG
Cuts at: 2001 2130 2001
Size: 129 6622
PvuII CAG'CTG
Cuts at: 2170 3970 2170
Size: 1800 4951
SacI G_AGCT'C
Cuts at: 3609 3609
Size: 6751
SacII CC_GC'GG
Cuts at: 3288 3325 3288
Size: 37 6714
SalI G'TCGA_C
Cuts at: 1936 4093 1936
Size: 2157 4594
SanDI GG'GwC_CC
Cuts at: 2296 2296
Size: 6751
SbfI CC_TGCA'GG
Cuts at: 4103 4103
Size: 6751
ScaI AGT'ACT
Cuts at: 4999 6711 4999
Size: 1712 5039
SfcI C'TryA_G
Cuts at: 826 1045 2032 2084 2945 3824 3965 4099 5234
Size: 219 987 52 861 879 141 134 1135
Cuts at: 5234 5915 6106 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 987 879 861 681 219 191
141 134 52
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6751
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6751
SmaI CCC'GGG
Cuts at: 1872 4078 1872
Size: 2206 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2916 3535 4855 5756 6018 820
Size: 197 926 973 619 1320 901 262 1553
Fragments arranged by size:
1553 1320 973 926 901 619 262 197
SnaBI TAC'GTA
Cuts at: 493 4542 493
Size: 4049 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6751
SphI G_CATG'C
Cuts at: 1132 2753 4109 1132
Size: 1621 1356 3774
Fragments arranged by size:
3774 1621 1356
SspI AAT'ATT
Cuts at: 5 52 4675 5
Size: 47 4623 2081
Fragments arranged by size:
4623 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6751
StyI C'CwwG_G
Cuts at: 513 1065 3432 3870 4190 513
Size: 552 2367 438 320 3074
Fragments arranged by size:
3074 2367 552 438 320
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2303 3056 3395 3507 4778 4795 4948
Size: 1255 723 753 339 112 1271 17 153
Cuts at: 4948 5133 5578 325
Size: 185 445 1498
Fragments arranged by size:
1498 1271 1255 753 723 445 339 185
153 112 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2097 4997 6709
Size: 274 80 33 51 1443 120 2900 1712
Cuts at: 6709 96
Size: 138
Fragments arranged by size:
2900 1712 1443 274 138 120 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6711 6743 6711
Size: 32 6719
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6751
XbaI T'CTAG_A
Cuts at: 4087 4087
Size: 6751
XhoI C'TCGA_G
Cuts at: 1943 2916 1943
Size: 973 5778
XmaI C'CCGG_G
Cuts at: 1870 4076 1870
Size: 2206 4545
XmnI GAAnn'nnTTC
Cuts at: 4880 4880
Size: 6751
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4559 276
Size: 53 83 186 936 3025 2468
Fragments arranged by size:
3025 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NruI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SacII SalI SanDI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NsiI PacI PmlI PspXI PsrI RsrII
SapI SexAI SfiI SfoI SrfI SwaI XcmI