Restriction map for
pFN21AE6710
(Circular) (Six-base) MAPSORT of: pFN21AE6710.seq Check: 8365 from: 1 to: 6781
pFN21AE6710 6781 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3045 3068 3812 3045
Size: 23 744 6014
Fragments arranged by size:
6014 744 23
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2451 4591 278
Size: 53 83 186 936 915 2140 2468
Fragments arranged by size:
2468 2140 936 915 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3867 4124 1937
Size: 1930 257 4594
Fragments arranged by size:
4594 1930 257
Acc65I G'GTAC_C
Cuts at: 4102 4102
Size: 6781
AclI AA'CG_TT
Cuts at: 1563 4908 5281 1563
Size: 3345 373 3063
Fragments arranged by size:
3345 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4041 4844 6228 6496 1632
Size: 2409 803 1384 268 1917
Fragments arranged by size:
2409 1917 1384 803 268
AfeI AGC'GCT
Cuts at: 2230 2230
Size: 6781
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6584
AflIII A'CryG_T
Cuts at: 1203 5680 6657 1203
Size: 4477 977 1327
Fragments arranged by size:
4477 1327 977
AgeI A'CCGG_T
Cuts at: 5612 5612
Size: 6781
AhdI GACnn_n'nnGTC
Cuts at: 5510 5510
Size: 6781
AleI CACnn'nnGTG
Cuts at: 1166 3455 1166
Size: 2289 4492
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6781
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2198 2230 2198
Size: 32 6749
AlwNI CAG_nnn'CTG
Cuts at: 2593 2869 3007 3386 3946 4524 6096 2593
Size: 276 138 379 560 578 1572 3278
Fragments arranged by size:
3278 1572 578 560 379 276 138
ApaI G_GGCC'C
Cuts at: 2487 2554 2487
Size: 67 6714
ApaLI G'TGCA_C
Cuts at: 4838 5994 4838
Size: 1156 5625
ApoI r'AATT_y
Cuts at: 1493 4090 4316 4468 6577 1493
Size: 2597 226 152 2109 1697
Fragments arranged by size:
2597 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5335 160
Size: 872 4303 1606
Fragments arranged by size:
4303 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6781
AvaI C'yCGr_G
Cuts at: 1870 1943 2381 2467 4106 1870
Size: 73 438 86 1639 4545
Fragments arranged by size:
4545 1639 438 86 73
BamHI G'GATC_C
Cuts at: 4111 4478 4111
Size: 367 6414
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2174 2313 2432 2819 2964
Size: 325 217 627 387 139 119 387 145
Cuts at: 2964 3597 3653 3839 4102 5557 618
Size: 633 56 186 263 1455 1842
Fragments arranged by size:
1842 1455 633 627 387 387 325 263
217 186 145 139 119 56
BanII G_rGCy'C
Cuts at: 1391 2487 2554 2705 4100 1391
Size: 1096 67 151 1395 4072
Fragments arranged by size:
4072 1395 1096 151 67
BbeI G_GCGC'C
Cuts at: 2178 2436 3843 2178
Size: 258 1407 5116
Fragments arranged by size:
5116 1407 258
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2728 4068 928
Size: 971 829 1340 3641
Fragments arranged by size:
3641 1340 971 829
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3631 3665 3877 3911 4972
Size: 34 50 34 1610 34 212 34 1061
Cuts at: 4972 5006 1903
Size: 34 3678
Fragments arranged by size:
3678 1610 1061 212 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4673 5883 4673
Size: 1210 5571
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6781
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2049 2331 3260
Size: 107 122 71 631 605 377 282 929
Cuts at: 3260 5392 136
Size: 2132 1525
Fragments arranged by size:
2132 1525 929 631 605 377 282 122
107 71
BglII A'GATC_T
Cuts at: 2124 6776 2124
Size: 4652 2129
BlpI GC'TnA_GC
Cuts at: 3242 3391 3992 4198 3242
Size: 149 601 206 5825
Fragments arranged by size:
5825 601 206 149
Bme1580I G_kGCm'C
Cuts at: 1790 2168 2308 2316 2487 2554 4842 5998 1790
Size: 378 140 8 171 67 2288 1156 2573
Fragments arranged by size:
2573 2288 1156 378 171 140 67 8
BmgBI CAC'GTC
Cuts at: 1318 2236 1318
Size: 918 5863
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3582 4512 5470
Size: 416 53 83 399 24 2165 930 958
Cuts at: 5470 442
Size: 1753
Fragments arranged by size:
2165 1753 958 930 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2253 1056
Size: 1197 5584
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2225 2993 3152 3438 3835 5441 1239
Size: 986 768 159 286 397 1606 2579
Fragments arranged by size:
2579 1606 986 768 397 286 159
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3695 4906 5771 6069 3695
Size: 1211 865 298 4407
Fragments arranged by size:
4407 1211 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2929 4016 5444 882
Size: 617 1430 1087 1428 2219
Fragments arranged by size:
2219 1430 1428 1087 617
BsaAI yAC'GTr
Cuts at: 493 1204 2620 2725 3936 4572 493
Size: 711 1416 105 1211 636 2702
Fragments arranged by size:
2702 1416 1211 711 636 105
BsaBI GATnn'nnATC
Cuts at: 3635 6775 3635
Size: 3140 3641
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2175 2433 2448 2598
Size: 53 83 186 936 642 258 15 150
Cuts at: 2598 3840 4588 4970 5605 275
Size: 1242 748 382 635 1451
Fragments arranged by size:
1451 1242 936 748 642 635 382 258
186 150 83 53 15
BsaWI w'CCGG_w
Cuts at: 2511 2767 5214 5612 5886 6033 6444 2511
Size: 256 2447 398 274 147 411 2848
Fragments arranged by size:
2848 2447 411 398 274 256 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2198 2228 3125 3155 1178
Size: 30 165 30 795 30 897 30 4804
Fragments arranged by size:
4804 897 795 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2626 3731 1186
Size: 1440 1105 4236
Fragments arranged by size:
4236 1440 1105
BseYI C'CCAG_C
Cuts at: 2356 2427 2945 3350 5984 6480 2356
Size: 71 518 405 2634 496 2657
Fragments arranged by size:
2657 2634 518 496 405 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2606 1163
Size: 573 870 5338
Fragments arranged by size:
5338 870 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2044 2321 4231 4992 6020 1540
Size: 275 186 43 277 1910 761 1028 2301
Fragments arranged by size:
2301 1910 1028 761 277 275 186 43
BsiHKAI G_wGCw'C
Cuts at: 2562 3821 3998 4842 4927 5998 2562
Size: 1259 177 844 85 1071 3345
Fragments arranged by size:
3345 1259 1071 844 177 85
BsmI GAATG_Cn'
Cuts at: 3437 4292 4385 3437
Size: 855 93 5833
Fragments arranged by size:
5833 855 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2605 3316 2605
Size: 711 6070
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2168 2308 2316 2487 2554 2562 2705
Size: 399 378 140 8 171 67 8 143
Cuts at: 2705 3821 3998 4100 4842 4927 5998 1391
Size: 1116 177 102 742 85 1071 2174
Fragments arranged by size:
2174 1116 1071 742 399 378 177 171
143 140 102 85 67 8 8
BspEI T'CCGG_A
Cuts at: 2767 6444 2767
Size: 3677 3104
BspHI T'CATG_A
Cuts at: 4670 6400 4670
Size: 1730 5051
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2229 3045 3068 3812 4043 4136 844
Size: 1385 816 23 744 231 93 3489
Fragments arranged by size:
3489 1385 816 744 231 93 23
BsrBI CCG'CTC
Cuts at: 1394 1823 2049 4668 1394
Size: 429 226 2619 3507
Fragments arranged by size:
3507 2619 429 226
BsrDI GCAATG_nn'
Cuts at: 65 1248 5276 5450 65
Size: 1183 4028 174 1396
Fragments arranged by size:
4028 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2316 5425 5612 1700
Size: 110 506 3109 187 2869
Fragments arranged by size:
3109 2869 506 187 110
BsrGI T'GTAC_A
Cuts at: 96 3924 96
Size: 3828 2953
BssHII G'CGCG_C
Cuts at: 1925 2361 1925
Size: 436 6345
BssSI C'ACGA_G
Cuts at: 3980 4841 5853 3980
Size: 861 1012 4908
Fragments arranged by size:
4908 1012 861
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2911 794
Size: 2117 4664
BstBI TT'CG_AA
Cuts at: 5585 5585
Size: 6781
BstEII G'GTnAC_C
Cuts at: 1184 3025 1184
Size: 1841 4940
BstXI CCAn_nnnn'nTGG
Cuts at: 3176 3271 3176
Size: 95 6686
BstYI r'GATC_y
Cuts at: 1972 2124 2421 4111 4478 4863 4880 6332 6418
Size: 152 297 1690 367 385 17 1452 86
Cuts at: 6418 6430 6776 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1690 1452 385 367 346 297 152
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2960 3947 2960
Size: 987 5794
BtgI C'CryG_G
Cuts at: 513 1065 2892 3567 3657 513
Size: 552 1827 675 90 3637
Fragments arranged by size:
3637 1827 675 552 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3874 3880 487
Size: 683 56 2648 6 3388
Fragments arranged by size:
3388 2648 683 56 6
BtsI GCAGTG_nn'
Cuts at: 2992 3099 3297 3947 4297 5091 5111 2992
Size: 107 198 650 350 794 20 4662
Fragments arranged by size:
4662 794 650 350 198 107 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6781
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6746
DraI TTT'AAA
Cuts at: 4085 4085
Size: 6781
DraIII CAC_nnn'GTG
Cuts at: 3936 3936
Size: 6781
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3863 5788 809
Size: 507 2547 1925 1802
Fragments arranged by size:
2547 1925 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2041 2318 3072
Size: 54 1425 50 275 18 211 277 754
Cuts at: 3072 3273 4228 5117 6746 8
Size: 201 955 889 1629 43
Fragments arranged by size:
1629 1425 955 889 754 277 275 211
201 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2041 2318 4228 1537
Size: 275 229 277 1910 4090
Fragments arranged by size:
4090 1910 277 275 229
EarI CTCTTCn'nnn_
Cuts at: 1353 2291 2834 2842 3163 3326 4711 1353
Size: 938 543 8 321 163 1385 3423
Fragments arranged by size:
3423 1385 938 543 321 163 8
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5368 5742 5888 387
Size: 1064 138 3779 374 146 1280
Fragments arranged by size:
3779 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2225 2993 3152 3438 3835 4041 4844
Size: 393 593 768 159 286 397 206 803
Cuts at: 4844 5441 6228 6496 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 803 787 768 597 593 397 393
286 268 206 159
EcoO109I rG'GnC_Cy
Cuts at: 2483 2550 2956 2483
Size: 67 406 6308
Fragments arranged by size:
6308 406 67
EcoRI G'AATT_C
Cuts at: 4090 4468 4090
Size: 378 6403
FspI TGC'GCA
Cuts at: 2455 5287 2455
Size: 2832 3949
HaeII r_GCGC'y
Cuts at: 1678 2178 2232 2279 2357 2378 2436 3843 5928
Size: 500 54 47 78 21 58 1407 2085
Cuts at: 5928 1678
Size: 2531
Fragments arranged by size:
2531 2085 1407 500 78 58 54 47
21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3392 3424 5422 5454 5496
Size: 32 135 32 1820 32 1998 32 42
Cuts at: 5496 5528 1373
Size: 32 2626
Fragments arranged by size:
2626 1998 1820 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3286 4125 4371 669
Size: 988 281 1348 839 246 3079
Fragments arranged by size:
3079 1348 988 839 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6781
HpaI GTT'AAC
Cuts at: 4371 4371
Size: 6781
KasI G'GCGC_C
Cuts at: 2174 2432 3839 2174
Size: 258 1407 5116
Fragments arranged by size:
5116 1407 258
KpnI G_GTAC'C
Cuts at: 4106 4106
Size: 6781
MfeI C'AATT_G
Cuts at: 4380 4380
Size: 6781
MluI A'CGCG_T
Cuts at: 6657 6657
Size: 6781
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2139 5895 6079 1305
Size: 834 3756 184 2007
Fragments arranged by size:
3756 2007 834 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3275 10
Size: 54 1425 343 1443 3516
Fragments arranged by size:
3516 1443 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2348 3269 3455 4739 5098 5257 518
Size: 648 1182 921 186 1284 359 159 2042
Fragments arranged by size:
2042 1284 1182 921 648 359 186 159
MspA1I CmG'CkG
Cuts at: 1606 2427 2781 3007 3172 4197 4874 6022 6267
Size: 821 354 226 165 1025 677 1148 245
Cuts at: 6267 1606
Size: 2120
Fragments arranged by size:
2120 1148 1025 821 677 354 245 226
165
NarI GG'CG_CC
Cuts at: 2175 2433 3840 2175
Size: 258 1407 5116
Fragments arranged by size:
5116 1407 258
NcoI C'CATG_G
Cuts at: 513 1065 2892 3657 513
Size: 552 1827 765 3637
Fragments arranged by size:
3637 1827 765 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6781
NheI G'CTAG_C
Cuts at: 1052 2249 1052
Size: 1197 5584
NotI GC'GGCC_GC
Cuts at: 4228 4228
Size: 6781
NspI r_CATG'y
Cuts at: 1132 1229 4139 5684 1132
Size: 97 2910 1545 2229
Fragments arranged by size:
2910 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2593 3256 1348
Size: 1245 663 4873
Fragments arranged by size:
4873 1245 663
PciI A'CATG_T
Cuts at: 5680 5680
Size: 6781
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3461 3936 1108
Size: 2353 475 3953
Fragments arranged by size:
3953 2353 475
PfoI T'CCnGG_A
Cuts at: 3574 3574
Size: 6781
PmeI GTTT'AAAC
Cuts at: 4085 4085
Size: 6781
PmlI CAC'GTG
Cuts at: 2620 3936 2620
Size: 1316 5465
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2198 2230 4850 4882 6363 6395 2198
Size: 32 2620 32 1481 32 2584
Fragments arranged by size:
2620 2584 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2956 2956
Size: 6781
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6781
PsiI TTA'TAA
Cuts at: 4351 4351
Size: 6781
PspOMI G'GGCC_C
Cuts at: 2483 2550 2483
Size: 67 6714
PstI C_TGCA'G
Cuts at: 830 2061 2226 3644 4133 830
Size: 1231 165 1418 489 3478
Fragments arranged by size:
3478 1418 1231 489 165
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6781
PvuII CAG'CTG
Cuts at: 3007 3172 3007
Size: 165 6616
SalI G'TCGA_C
Cuts at: 1936 4123 1936
Size: 2187 4594
SbfI CC_TGCA'GG
Cuts at: 4133 4133
Size: 6781
ScaI AGT'ACT
Cuts at: 5029 6741 5029
Size: 1712 5069
SexAI A'CCwGG_T
Cuts at: 2181 3288 2181
Size: 1107 5674
SfcI C'TryA_G
Cuts at: 826 1045 2057 2222 3640 4129 5264 5945 6136
Size: 219 1012 165 1418 489 1135 681 191
Cuts at: 6136 826
Size: 1471
Fragments arranged by size:
1471 1418 1135 1012 681 489 219 191
165
SfoI GGC'GCC
Cuts at: 2176 2434 3841 2176
Size: 258 1407 5116
Fragments arranged by size:
5116 1407 258
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6781
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6781
SmaI CCC'GGG
Cuts at: 1872 4108 1872
Size: 2236 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3710 4885 5786 6048 820
Size: 197 926 1767 1175 901 262 1553
Fragments arranged by size:
1767 1553 1175 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4572 493
Size: 4079 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6781
SphI G_CATG'C
Cuts at: 1132 4139 1132
Size: 3007 3774
SspI AAT'ATT
Cuts at: 5 52 4705 5
Size: 47 4653 2081
Fragments arranged by size:
4653 2081 47
StuI AGG'CCT
Cuts at: 1517 3608 1517
Size: 2091 4690
StyI C'CwwG_G
Cuts at: 513 1065 2892 3657 4220 513
Size: 552 1827 765 563 3074
Fragments arranged by size:
3074 1827 765 563 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2228 3585 4040 4808 4825 4978 5163
Size: 1255 648 1357 455 768 17 153 185
Cuts at: 5163 5608 325
Size: 445 1498
Fragments arranged by size:
1498 1357 1255 768 648 455 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2400 2532 3924
Size: 274 80 33 51 1443 423 132 1392
Cuts at: 3924 5027 6739 96
Size: 1103 1712 138
Fragments arranged by size:
1712 1443 1392 1103 423 274 138 132
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6741 6773 6741
Size: 32 6749
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6781
XbaI T'CTAG_A
Cuts at: 4117 4117
Size: 6781
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2722 2722
Size: 6781
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6781
XmaI C'CCGG_G
Cuts at: 1870 4106 1870
Size: 2236 4545
XmnI GAAnn'nnTTC
Cuts at: 4910 4910
Size: 6781
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2449 4589 276
Size: 53 83 186 936 915 2140 2468
Fragments arranged by size:
2468 2140 936 915 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI
BstZ17I EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacI
SacII SanDI SapI SfiI SrfI SwaI