Restriction map for
pFN21AE6706
(Circular) (Six-base) MAPSORT of: pFN21AE6706.seq Check: 4031 from: 1 to: 7717
pFN21AE6706 7717 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2035 2886 2035
Size: 851 6866
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5527 278
Size: 53 83 186 936 3991 2468
Fragments arranged by size:
3991 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2831 3102 3516 4341 5060 1937
Size: 894 271 414 825 719 4594
Fragments arranged by size:
4594 894 825 719 414 271
Acc65I G'GTAC_C
Cuts at: 5038 5038
Size: 7717
AclI AA'CG_TT
Cuts at: 1563 2533 5844 6217 1563
Size: 970 3311 373 3063
Fragments arranged by size:
3311 3063 970 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2350 3266 3384 3780 4218 4373 4946 5780
Size: 718 916 118 396 438 155 573 834
Cuts at: 5780 7164 7432 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 916 834 718 573 438 396
268 155 118
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7520
AflIII A'CryG_T
Cuts at: 1203 6616 7593 1203
Size: 5413 977 1327
Fragments arranged by size:
5413 1327 977
AgeI A'CCGG_T
Cuts at: 2073 2972 6548 2073
Size: 899 3576 3242
Fragments arranged by size:
3576 3242 899
AhdI GACnn_n'nnGTC
Cuts at: 6446 6446
Size: 7717
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7717
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7717
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2649 2681 4161 4193 2649
Size: 32 1480 32 6173
Fragments arranged by size:
6173 1480 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2155 2878 3362 5460 7032 2155
Size: 723 484 2098 1572 2840
Fragments arranged by size:
2840 2098 1572 723 484
ApaLI G'TGCA_C
Cuts at: 5774 6930 5774
Size: 1156 6561
ApoI r'AATT_y
Cuts at: 1493 2510 2730 2998 3463 5026 5252 5404 7513
Size: 1017 220 268 465 1563 226 152 2109
Cuts at: 7513 1493
Size: 1697
Fragments arranged by size:
2109 1697 1563 1017 465 268 226 220
152
AseI AT'TA_AT
Cuts at: 160 1032 2502 3821 3834 3966 6271 160
Size: 872 1470 1319 13 132 2305 1606
Fragments arranged by size:
2305 1606 1470 1319 872 132 13
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7717
AvaI C'yCGr_G
Cuts at: 1870 1943 5042 1870
Size: 73 3099 4545
Fragments arranged by size:
4545 3099 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3408 3441 3408
Size: 33 7684
BamHI G'GATC_C
Cuts at: 5047 5414 5047
Size: 367 7350
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2050 3672 5038 6493 618
Size: 325 217 627 263 1622 1366 1455 1842
Fragments arranged by size:
1842 1622 1455 1366 627 325 263 217
BanII G_rGCy'C
Cuts at: 1391 5036 1391
Size: 3645 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3388 4665 928
Size: 971 1489 1277 3980
Fragments arranged by size:
3980 1489 1277 971
BbvCI CC'TCA_GC
Cuts at: 3450 4329 3450
Size: 879 6838
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5908 5942 1903
Size: 34 3971 34 3678
Fragments arranged by size:
3971 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2330 3120 3997 5609 6819 2330
Size: 790 877 1612 1210 3228
Fragments arranged by size:
3228 1612 1210 877 790
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7717
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6328 136
Size: 107 122 71 631 605 4656 1525
Fragments arranged by size:
4656 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4069 4576 7712 4069
Size: 507 3136 4074
Fragments arranged by size:
4074 3136 507
BlpI GC'TnA_GC
Cuts at: 3915 4356 5134 3915
Size: 441 778 6498
Fragments arranged by size:
6498 778 441
Bme1580I G_kGCm'C
Cuts at: 1790 2055 3675 3933 5778 6934 1790
Size: 265 1620 258 1845 1156 2573
Fragments arranged by size:
2573 1845 1620 1156 265 258
BmgBI CAC'GTC
Cuts at: 1318 3157 4531 1318
Size: 1839 1374 4504
Fragments arranged by size:
4504 1839 1374
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5448 6406 442
Size: 416 53 83 399 24 4031 958 1753
Fragments arranged by size:
4031 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7717
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2465 2530 4464 2465
Size: 65 1934 5718
Fragments arranged by size:
5718 1934 65
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2084 2241 2305 2657 2846 2933 3648 4447
Size: 845 157 64 352 189 87 715 799
Cuts at: 4447 6377 1239
Size: 1930 2579
Fragments arranged by size:
2579 1930 845 799 715 352 189 157
87 64
Bpu10I CC'TnA_GC
Cuts at: 3450 4308 4329 3450
Size: 858 21 6838
Fragments arranged by size:
6838 858 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2762 5842 6707 7005 2762
Size: 3080 865 298 3474
Fragments arranged by size:
3474 3080 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2364 2436 6380 882
Size: 617 865 72 3944 2219
Fragments arranged by size:
3944 2219 865 617 72
BsaAI yAC'GTr
Cuts at: 493 1204 2270 5508 493
Size: 711 1066 3238 2702
Fragments arranged by size:
3238 2702 1066 711
BsaBI GATnn'nnATC
Cuts at: 7711 7711
Size: 7717
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5524 5906 6541 275
Size: 53 83 186 936 3991 382 635 1451
Fragments arranged by size:
3991 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2073 2972 4797 6150 6548 6822 6969 7380 2073
Size: 899 1825 1353 398 274 147 411 2410
Fragments arranged by size:
2410 1825 1353 899 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2649 2679 2931 2961 3212
Size: 30 165 30 1246 30 252 30 251
Cuts at: 3212 3242 1178
Size: 30 5653
Fragments arranged by size:
5653 1246 252 251 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3233 1186
Size: 2047 5670
BseYI C'CCAG_C
Cuts at: 2185 3455 3705 6920 7416 2185
Size: 1270 250 3215 496 2486
Fragments arranged by size:
3215 2486 1270 496 250
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2009 2030 2443 3217 1163
Size: 573 273 21 413 774 5663
Fragments arranged by size:
5663 774 573 413 273 21
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5167 5928 6956 1540
Size: 275 186 3166 761 1028 2301
Fragments arranged by size:
3166 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5778 5863 6934 5778
Size: 85 1071 6561
Fragments arranged by size:
6561 1071 85
BsmI GAATG_Cn'
Cuts at: 3558 3709 5228 5321 3558
Size: 151 1519 93 5954
Fragments arranged by size:
5954 1519 151 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2055 3675 3933 5036 5778 5863 6934
Size: 399 265 1620 258 1103 742 85 1071
Cuts at: 6934 1391
Size: 2174
Fragments arranged by size:
2174 1620 1103 1071 742 399 265 258
85
BspEI T'CCGG_A
Cuts at: 7380 7380
Size: 7717
BspHI T'CATG_A
Cuts at: 4777 4935 5606 7336 4777
Size: 158 671 1730 5158
Fragments arranged by size:
5158 1730 671 158
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2031 2035 2886 3183 5072 844
Size: 1187 4 851 297 1889 3489
Fragments arranged by size:
3489 1889 1187 851 297 4
BsrBI CCG'CTC
Cuts at: 1394 1823 5604 1394
Size: 429 3781 3507
Fragments arranged by size:
3781 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4463 6212 6386 65
Size: 1183 3215 1749 174 1396
Fragments arranged by size:
3215 1749 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2073 2144 2972 4670 6361 6548 1700
Size: 110 263 71 828 1698 1691 187 2869
Fragments arranged by size:
2869 1698 1691 828 263 187 110 71
BsrGI T'GTAC_A
Cuts at: 96 2016 2379 3501 96
Size: 1920 363 1122 4312
Fragments arranged by size:
4312 1920 1122 363
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7717
BssSI C'ACGA_G
Cuts at: 2310 3213 5777 6789 2310
Size: 903 2564 1012 3238
Fragments arranged by size:
3238 2564 1012 903
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2050 794
Size: 1256 6461
BstBI TT'CG_AA
Cuts at: 6521 6521
Size: 7717
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7717
BstXI CCAn_nnnn'nTGG
Cuts at: 3619 3619
Size: 7717
BstYI r'GATC_y
Cuts at: 1972 4069 4480 4576 5047 5414 5799 5816 7268
Size: 2097 411 96 471 367 385 17 1452
Cuts at: 7268 7354 7366 7712 1972
Size: 86 12 346 1977
Fragments arranged by size:
2097 1977 1452 471 411 385 367 346
96 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2165 4520 513
Size: 552 939 161 2355 3710
Fragments arranged by size:
3710 2355 939 552 161
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6978
Fragments arranged by size:
6978 683 56
BtsI GCAGTG_nn'
Cuts at: 3185 3205 4056 5233 6027 6047 3185
Size: 20 851 1177 794 20 4855
Fragments arranged by size:
4855 1177 851 794 20 20
ClaI AT'CG_AT
Cuts at: 1554 4022 1554
Size: 2468 5249
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3132 3167 529
Size: 35 2568 35 5079
Fragments arranged by size:
5079 2568 35 35
DraI TTT'AAA
Cuts at: 3328 5021 3328
Size: 1693 6024
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2618 6724 809
Size: 507 1302 4106 1802
Fragments arranged by size:
4106 1802 1302 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2142 2146 5164
Size: 54 1425 50 275 18 312 4 3018
Cuts at: 5164 6053 7682 8
Size: 889 1629 43
Fragments arranged by size:
3018 1629 1425 889 312 275 54 50
43 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 5164 1537
Size: 275 3352 4090
Fragments arranged by size:
4090 3352 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2238 4785 5647 1353
Size: 885 2547 862 3423
Fragments arranged by size:
3423 2547 885 862
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6304 6678 6824 387
Size: 1064 138 4715 374 146 1280
Fragments arranged by size:
4715 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2084 2241 2305 2350 2657 2846 2933
Size: 393 452 157 64 45 307 189 87
Cuts at: 2933 3266 3384 3648 3780 4218 4373 4447 4946
Size: 333 118 264 132 438 155 74 499
Cuts at: 4946 5780 6377 7164 7432 1239
Size: 834 597 787 268 1524
Fragments arranged by size:
1524 834 787 597 499 452 438 393
333 307 268 264 189 157 155 132
118 87 74 64 45
EcoNI CCTnn'n_nnAGG
Cuts at: 2436 2436
Size: 7717
EcoO109I rG'GnC_Cy
Cuts at: 2407 2645 3223 2407
Size: 238 578 6901
Fragments arranged by size:
6901 578 238
EcoRI G'AATT_C
Cuts at: 2510 5026 5404 2510
Size: 2516 378 4823
Fragments arranged by size:
4823 2516 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2462 2462
Size: 7717
FseI GG_CCGG'CC
Cuts at: 2148 2148
Size: 7717
FspI TGC'GCA
Cuts at: 6223 6223
Size: 7717
HaeII r_GCGC'y
Cuts at: 1678 6864 1678
Size: 5186 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2677 2709 2894 2926 2962
Size: 32 135 32 1105 32 185 32 36
Cuts at: 2962 2994 3807 3839 6358 6390 6432 6464 1373
Size: 32 813 32 2519 32 42 32 2626
Fragments arranged by size:
2626 2519 1105 813 185 135 42 36
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5061 5307 669
Size: 988 281 3123 246 3079
Fragments arranged by size:
3123 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7717
HpaI GTT'AAC
Cuts at: 5307 5307
Size: 7717
KpnI G_GTAC'C
Cuts at: 5042 5042
Size: 7717
MfeI C'AATT_G
Cuts at: 2238 4902 5316 2238
Size: 2664 414 4639
Fragments arranged by size:
4639 2664 414
MluI A'CGCG_T
Cuts at: 7593 7593
Size: 7717
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2569 4205 6831 7015 1305
Size: 1264 1636 2626 184 2007
Fragments arranged by size:
2626 2007 1636 1264 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5895
Fragments arranged by size:
5895 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4086 5675 6034 6193 518
Size: 648 2920 1589 359 159 2042
Fragments arranged by size:
2920 2042 1589 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2014 2167 2953 3619 5133 5810 6958 7203
Size: 408 153 786 666 1514 677 1148 245
Cuts at: 7203 1606
Size: 2120
Fragments arranged by size:
2120 1514 1148 786 677 666 408 245
153
NaeI GCC'GGC
Cuts at: 2146 4672 2146
Size: 2526 5191
NcoI C'CATG_G
Cuts at: 513 1065 2004 4520 513
Size: 552 939 2516 3710
Fragments arranged by size:
3710 2516 939 552
NdeI CA'TA_TG
Cuts at: 387 4768 387
Size: 4381 3336
NgoMIV G'CCGG_C
Cuts at: 2144 4670 2144
Size: 2526 5191
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7717
NotI GC'GGCC_GC
Cuts at: 5164 5164
Size: 7717
NspI r_CATG'y
Cuts at: 1132 1229 4081 4371 5075 6620 1132
Size: 97 2852 290 704 1545 2229
Fragments arranged by size:
2852 2229 1545 704 290 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7717
PciI A'CATG_T
Cuts at: 6616 6616
Size: 7717
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4955 1108
Size: 3847 3870
PmeI GTTT'AAAC
Cuts at: 5021 5021
Size: 7717
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2649 2681 5786 5818 7299 7331 2649
Size: 32 3105 32 1481 32 3035
Fragments arranged by size:
3105 3035 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3223 3223
Size: 7717
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7717
PsiI TTA'TAA
Cuts at: 5287 5287
Size: 7717
PstI C_TGCA'G
Cuts at: 830 3180 5069 830
Size: 2350 1889 3478
Fragments arranged by size:
3478 2350 1889
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7717
PvuII CAG'CTG
Cuts at: 2014 2953 3619 2014
Size: 939 666 6112
Fragments arranged by size:
6112 939 666
RsrII CG'GwC_CG
Cuts at: 2071 2071
Size: 7717
SacII CC_GC'GG
Cuts at: 2168 2168
Size: 7717
SalI G'TCGA_C
Cuts at: 1936 5059 1936
Size: 3123 4594
SbfI CC_TGCA'GG
Cuts at: 5069 5069
Size: 7717
ScaI AGT'ACT
Cuts at: 3135 5965 7677 3135
Size: 2830 1712 3175
Fragments arranged by size:
3175 2830 1712
SfcI C'TryA_G
Cuts at: 826 1045 2209 2884 3176 3444 3520 3943 4495
Size: 219 1164 675 292 268 76 423 552
Cuts at: 4495 5065 6200 6881 7072 826
Size: 570 1135 681 191 1471
Fragments arranged by size:
1471 1164 1135 681 675 570 552 423
292 268 219 191 76
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7717
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7717
SmaI CCC'GGG
Cuts at: 1872 5044 1872
Size: 3172 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2741 5821 6722 6984 820
Size: 197 926 798 3080 901 262 1553
Fragments arranged by size:
3080 1553 926 901 798 262 197
SnaBI TAC'GTA
Cuts at: 493 5508 493
Size: 5015 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7717
SphI G_CATG'C
Cuts at: 1132 5075 1132
Size: 3943 3774
SspI AAT'ATT
Cuts at: 5 52 2861 3964 5641 5
Size: 47 2809 1103 1677 2081
Fragments arranged by size:
2809 2081 1677 1103 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7717
StyI C'CwwG_G
Cuts at: 513 1065 2004 3676 4041 4520 5156 513
Size: 552 939 1672 365 479 636 3074
Fragments arranged by size:
3074 1672 939 636 552 479 365
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3406 3447 5744 5761 5914 6099 6544
Size: 1255 1826 41 2297 17 153 185 445
Cuts at: 6544 325
Size: 1498
Fragments arranged by size:
2297 1826 1498 1255 445 185 153 41
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2016 2379 3133
Size: 274 80 33 51 1443 39 363 754
Cuts at: 3133 3501 4419 5963 7675 96
Size: 368 918 1544 1712 138
Fragments arranged by size:
1712 1544 1443 918 754 368 363 274
138 80 51 39 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4212 4244 7677 7709 4212
Size: 32 3433 32 4220
Fragments arranged by size:
4220 3433 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7717
XbaI T'CTAG_A
Cuts at: 5053 5053
Size: 7717
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4048 4048
Size: 7717
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7717
XmaI C'CCGG_G
Cuts at: 1870 5042 1870
Size: 3172 4545
XmnI GAAnn'nnTTC
Cuts at: 2860 3369 4931 5846 2860
Size: 509 1562 915 4731
Fragments arranged by size:
4731 1562 915 509
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5525 276
Size: 53 83 186 936 3991 2468
Fragments arranged by size:
3991 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII RsrII SacII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BbeI BfrBI BsiWI BsmBI
BstZ17I Bsu36I DraIII EcoICRI EcoRV FspAI KasI NarI
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI