Restriction map for
pFN21AE6705
(Circular) (Six-base) MAPSORT of: pFN21AE6705.seq Check: 7990 from: 1 to: 7006
pFN21AE6705 7006 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4249 4249
Size: 7006
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4816 278
Size: 53 83 186 936 3280 2468
Fragments arranged by size:
3280 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4349 1937
Size: 2412 4594
Acc65I G'GTAC_C
Cuts at: 4327 4327
Size: 7006
AclI AA'CG_TT
Cuts at: 1563 5133 5506 1563
Size: 3570 373 3063
Fragments arranged by size:
3570 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3272 5069 6453 6721 1632
Size: 1640 1797 1384 268 1917
Fragments arranged by size:
1917 1797 1640 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6809
AflIII A'CryG_T
Cuts at: 1203 2070 5905 6882 1203
Size: 867 3835 977 1327
Fragments arranged by size:
3835 1327 977 867
AgeI A'CCGG_T
Cuts at: 5837 5837
Size: 7006
AhdI GACnn_n'nnGTC
Cuts at: 5735 5735
Size: 7006
AleI CACnn'nnGTG
Cuts at: 1166 2804 1166
Size: 1638 5368
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2697 2714 1184
Size: 1513 17 5476
Fragments arranged by size:
5476 1513 17
AlwNI CAG_nnn'CTG
Cuts at: 3844 4302 4749 6321 3844
Size: 458 447 1572 4529
Fragments arranged by size:
4529 1572 458 447
ApaI G_GGCC'C
Cuts at: 3630 3630
Size: 7006
ApaLI G'TGCA_C
Cuts at: 5063 6219 5063
Size: 1156 5850
ApoI r'AATT_y
Cuts at: 1493 3948 4092 4315 4541 4693 6802 1493
Size: 2455 144 223 226 152 2109 1697
Fragments arranged by size:
2455 2109 1697 226 223 152 144
AseI AT'TA_AT
Cuts at: 160 1032 5560 160
Size: 872 4528 1606
Fragments arranged by size:
4528 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7006
AvaI C'yCGr_G
Cuts at: 1870 1943 3243 4037 4331 1870
Size: 73 1300 794 294 4545
Fragments arranged by size:
4545 1300 794 294 73
BamHI G'GATC_C
Cuts at: 4336 4703 4336
Size: 367 6639
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4069 4327 5782 618
Size: 325 217 627 2282 258 1455 1842
Fragments arranged by size:
2282 1842 1455 627 325 258 217
BanII G_rGCy'C
Cuts at: 1391 3630 4325 1391
Size: 2239 695 4072
Fragments arranged by size:
4072 2239 695
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2465 2560 3705 928
Size: 971 566 95 1145 4229
Fragments arranged by size:
4229 1145 971 566 95
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5197 5231 1903
Size: 34 3260 34 3678
Fragments arranged by size:
3678 3260 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3955 4898 6108 3955
Size: 943 1210 4853
Fragments arranged by size:
4853 1210 943
BclI T'GATC_A
Cuts at: 1548 2502 3067 1548
Size: 954 565 5487
Fragments arranged by size:
5487 954 565
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5617 136
Size: 107 122 71 631 605 3945 1525
Fragments arranged by size:
3945 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3767 7001 3767
Size: 3234 3772
BlpI GC'TnA_GC
Cuts at: 2537 4423 2537
Size: 1886 5120
Bme1580I G_kGCm'C
Cuts at: 1790 2843 3064 3630 5067 6223 1790
Size: 1053 221 566 1437 1156 2573
Fragments arranged by size:
2573 1437 1156 1053 566 221
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7006
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2335 3844 4000
Size: 416 53 83 399 24 918 1509 156
Cuts at: 4000 4737 5695 442
Size: 737 958 1753
Fragments arranged by size:
1753 1509 958 918 737 416 399 156
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7006
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3786 3786
Size: 7006
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2039 2111 2274 2441 2466 2855 2901 3132
Size: 800 72 163 167 25 389 46 231
Cuts at: 3132 3457 4245 4279 5666 1239
Size: 325 788 34 1387 2579
Fragments arranged by size:
2579 1387 800 788 389 325 231 167
163 72 46 34 25
Bpu10I CC'TnA_GC
Cuts at: 3539 3539
Size: 7006
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4071 5131 5996 6294 4071
Size: 1060 865 298 4783
Fragments arranged by size:
4783 1060 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2779 3989 5669 882
Size: 617 1280 1210 1680 2219
Fragments arranged by size:
2219 1680 1280 1210 617
BsaAI yAC'GTr
Cuts at: 493 1204 2362 3121 4797 493
Size: 711 1158 759 1676 2702
Fragments arranged by size:
2702 1676 1158 759 711
BsaBI GATnn'nnATC
Cuts at: 7000 7000
Size: 7006
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4813 5195 5830 275
Size: 53 83 186 936 3280 382 635 1451
Fragments arranged by size:
3280 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3639 5439 5837 6111 6258 6669 3639
Size: 1800 398 274 147 411 3976
Fragments arranged by size:
3976 1800 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3077 3105 3107 3135 3872
Size: 30 165 30 1674 28 2 28 737
Cuts at: 3872 3902 1178
Size: 30 4282
Fragments arranged by size:
4282 1674 737 165 30 30 30 28
28 2
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3453 3635 1186
Size: 2267 182 4557
Fragments arranged by size:
4557 2267 182
BseYI C'CCAG_C
Cuts at: 6209 6705 6209
Size: 496 6510
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2543 2643 1163
Size: 573 807 100 5526
Fragments arranged by size:
5526 807 573 100
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4456 5217 6245 1540
Size: 275 186 2455 761 1028 2301
Fragments arranged by size:
2455 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2380 2676 3639 4231 5067 5152 6223 2380
Size: 296 963 592 836 85 1071 3163
Fragments arranged by size:
3163 1071 963 836 592 296 85
BsmI GAATG_Cn'
Cuts at: 3933 4517 4610 3933
Size: 584 93 6329
Fragments arranged by size:
6329 584 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3478 3478
Size: 7006
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2380 2676 2843 3064 3630 3639 4231
Size: 399 590 296 167 221 566 9 592
Cuts at: 4231 4325 5067 5152 6223 1391
Size: 94 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 592 590 566 399 296
221 167 94 85 9
BspEI T'CCGG_A
Cuts at: 6669 6669
Size: 7006
BspHI T'CATG_A
Cuts at: 2530 2979 3861 4895 6625 2530
Size: 449 882 1034 1730 2911
Fragments arranged by size:
2911 1730 1034 882 449
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2332 2515 2665 4249 4361 844
Size: 1488 183 150 1584 112 3489
Fragments arranged by size:
3489 1584 1488 183 150 112
BsrBI CCG'CTC
Cuts at: 1394 1823 3611 4893 1394
Size: 429 1788 1282 3507
Fragments arranged by size:
3507 1788 1282 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2817 5501 5675 65
Size: 1183 1569 2684 174 1396
Fragments arranged by size:
2684 1569 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2085 5650 5837 1700
Size: 110 275 3565 187 2869
Fragments arranged by size:
3565 2869 275 187 110
BsrGI T'GTAC_A
Cuts at: 96 2164 3526 96
Size: 2068 1362 3576
Fragments arranged by size:
3576 2068 1362
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7006
BssSI C'ACGA_G
Cuts at: 3337 5066 6078 3337
Size: 1729 1012 4265
Fragments arranged by size:
4265 1729 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2657 3218 4063 794
Size: 1863 561 845 3737
Fragments arranged by size:
3737 1863 845 561
BstBI TT'CG_AA
Cuts at: 5810 5810
Size: 7006
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7006
BstXI CCAn_nnnn'nTGG
Cuts at: 2089 2806 2903 2089
Size: 717 97 6192
Fragments arranged by size:
6192 717 97
BstYI r'GATC_y
Cuts at: 1972 3364 3767 4336 4703 5088 5105 6557 6643
Size: 1392 403 569 367 385 17 1452 86
Cuts at: 6643 6655 7001 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1392 569 403 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2762 513
Size: 552 939 758 4757
Fragments arranged by size:
4757 939 758 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3490 487
Size: 683 56 2264 4003
Fragments arranged by size:
4003 2264 683 56
BtsI GCAGTG_nn'
Cuts at: 3037 3585 4522 5316 5336 3037
Size: 548 937 794 20 4707
Fragments arranged by size:
4707 937 794 548 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7006
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6971
DraI TTT'AAA
Cuts at: 4310 4310
Size: 7006
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6013 809
Size: 507 4697 1802
Fragments arranged by size:
4697 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2765 4453 5342
Size: 54 1425 50 275 18 935 1688 889
Cuts at: 5342 6971 8
Size: 1629 43
Fragments arranged by size:
1688 1629 1425 935 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4453 1537
Size: 275 2641 4090
Fragments arranged by size:
4090 2641 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2777 3158 4936 1353
Size: 1424 381 1778 3423
Fragments arranged by size:
3423 1778 1424 381
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5593 5967 6113 387
Size: 1064 138 4004 374 146 1280
Fragments arranged by size:
4004 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2039 2111 2274 2441 2466 2855 2901
Size: 393 407 72 163 167 25 389 46
Cuts at: 2901 3132 3272 3457 4245 4279 5069 5666 6453
Size: 231 140 185 788 34 790 597 787
Cuts at: 6453 6721 1239
Size: 268 1524
Fragments arranged by size:
1524 790 788 787 597 407 393 389
268 231 185 167 163 140 72 46
34 25
EcoNI CCTnn'n_nnAGG
Cuts at: 2895 4064 2895
Size: 1169 5837
EcoO109I rG'GnC_Cy
Cuts at: 2758 2885 2900 3626 3841 2758
Size: 127 15 726 215 5923
Fragments arranged by size:
5923 726 215 127 15
EcoRI G'AATT_C
Cuts at: 4315 4693 4315
Size: 378 6628
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2450 2450
Size: 7006
FspI TGC'GCA
Cuts at: 5512 5512
Size: 7006
HaeII r_GCGC'y
Cuts at: 1678 6153 1678
Size: 4475 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2850 2882 3356 3388 3429
Size: 32 135 32 1278 32 474 32 41
Cuts at: 3429 3461 3645 3677 3794 3826 4218 4250 5647
Size: 32 184 32 117 32 392 32 1397
Cuts at: 5647 5679 5721 5753 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1397 1278 474 392 184 135 117
42 41 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2410 4350 4596 669
Size: 988 281 472 1940 246 3079
Fragments arranged by size:
3079 1940 988 472 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7006
HpaI GTT'AAC
Cuts at: 4596 4596
Size: 7006
KpnI G_GTAC'C
Cuts at: 4331 4331
Size: 7006
MfeI C'AATT_G
Cuts at: 2352 4605 2352
Size: 2253 4753
MluI A'CGCG_T
Cuts at: 6882 6882
Size: 7006
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2045 2896 3688 6120 6304 1305
Size: 740 851 792 2432 184 2007
Fragments arranged by size:
2432 2007 851 792 740 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2767 10
Size: 54 1425 343 935 4249
Fragments arranged by size:
4249 1425 935 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2639 2804 3494 4964 5323 5482 518
Size: 648 1473 165 690 1470 359 159 2042
Fragments arranged by size:
2042 1473 1470 690 648 359 165 159
MspA1I CmG'CkG
Cuts at: 1606 3026 4422 5099 6247 6492 1606
Size: 1420 1396 677 1148 245 2120
Fragments arranged by size:
2120 1420 1396 1148 677 245
NaeI GCC'GGC
Cuts at: 2087 2087
Size: 7006
NcoI C'CATG_G
Cuts at: 513 1065 2004 2762 513
Size: 552 939 758 4757
Fragments arranged by size:
4757 939 758 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7006
NgoMIV G'CCGG_C
Cuts at: 2085 2085
Size: 7006
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7006
NotI GC'GGCC_GC
Cuts at: 4453 4453
Size: 7006
NspI r_CATG'y
Cuts at: 1132 1229 2418 4036 4364 5909 1132
Size: 97 1189 1618 328 1545 2229
Fragments arranged by size:
2229 1618 1545 1189 328 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7006
PciI A'CATG_T
Cuts at: 5905 5905
Size: 7006
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2297 1108
Size: 1189 5817
PmeI GTTT'AAAC
Cuts at: 4310 4310
Size: 7006
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5075 5107 6588 6620 5075
Size: 32 1481 32 5461
Fragments arranged by size:
5461 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2758 2885 3841 2758
Size: 127 956 5923
Fragments arranged by size:
5923 956 127
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7006
PsiI TTA'TAA
Cuts at: 4576 4576
Size: 7006
PspOMI G'GGCC_C
Cuts at: 3626 3626
Size: 7006
PstI C_TGCA'G
Cuts at: 830 2460 2703 4068 4260 4358 830
Size: 1630 243 1365 192 98 3478
Fragments arranged by size:
3478 1630 1365 243 192 98
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7006
PvuII CAG'CTG
Cuts at: 3026 3026
Size: 7006
SalI G'TCGA_C
Cuts at: 1936 4348 1936
Size: 2412 4594
SanDI GG'GwC_CC
Cuts at: 2758 2758
Size: 7006
SbfI CC_TGCA'GG
Cuts at: 4260 4358 4260
Size: 98 6908
ScaI AGT'ACT
Cuts at: 3915 5254 6966 3915
Size: 1339 1712 3955
Fragments arranged by size:
3955 1712 1339
SfcI C'TryA_G
Cuts at: 826 1045 2456 2699 2750 4064 4256 4354 5489
Size: 219 1411 243 51 1314 192 98 1135
Cuts at: 5489 6170 6361 826
Size: 681 191 1471
Fragments arranged by size:
1471 1411 1314 1135 681 243 219 192
191 98 51
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7006
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7006
SmaI CCC'GGG
Cuts at: 1872 4333 1872
Size: 2461 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4086 5110 6011 6273 820
Size: 197 926 2143 1024 901 262 1553
Fragments arranged by size:
2143 1553 1024 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4797 493
Size: 4304 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7006
SphI G_CATG'C
Cuts at: 1132 4364 1132
Size: 3232 3774
SspI AAT'ATT
Cuts at: 5 52 4930 5
Size: 47 4878 2081
Fragments arranged by size:
4878 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7006
StyI C'CwwG_G
Cuts at: 513 1065 2004 2580 2762 2896 3427 4445 513
Size: 552 939 576 182 134 531 1018 3074
Fragments arranged by size:
3074 1018 939 576 552 531 182 134
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3494 3666 4246 5033 5050 5203 5388
Size: 1255 1914 172 580 787 17 153 185
Cuts at: 5388 5833 325
Size: 445 1498
Fragments arranged by size:
1914 1498 1255 787 580 445 185 172
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2164 3526 3913
Size: 274 80 33 51 1443 187 1362 387
Cuts at: 3913 5252 6964 96
Size: 1339 1712 138
Fragments arranged by size:
1712 1443 1362 1339 387 274 187 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2505 2537 3077 3105 3109 3137 3291 3323 3340
Size: 32 540 28 4 28 154 32 17
Cuts at: 3340 3372 6966 6998 2505
Size: 32 3594 32 2513
Fragments arranged by size:
3594 2513 540 154 32 32 32 32
28 28 17 4
Tth111I GACn'n_nGTC
Cuts at: 1316 3669 3850 1316
Size: 2353 181 4472
Fragments arranged by size:
4472 2353 181
XbaI T'CTAG_A
Cuts at: 4342 4342
Size: 7006
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7006
XmaI C'CCGG_G
Cuts at: 1870 4331 1870
Size: 2461 4545
XmnI GAAnn'nnTTC
Cuts at: 5135 5135
Size: 7006
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4814 276
Size: 53 83 186 936 3280 2468
Fragments arranged by size:
3280 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI
KasI NarI NruI NsiI PacI PfoI PmlI PspXI
PsrI RsrII SacI SacII SapI SexAI SfiI SfoI
SrfI SwaI XcmI