Restriction map for
pFN21AE6694
(Circular) (Six-base) MAPSORT of: pFN21AE6694.seq Check: 711 from: 1 to: 5899
pFN21AE6694 5899 bp
With 182 enzymes: SgfI *
March 18, 2015 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2365 2365
Size: 5899
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3709 278
Size: 53 83 186 936 2173 2468
Fragments arranged by size:
2468 2173 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3242 1937
Size: 1305 4594
Acc65I G'GTAC_C
Cuts at: 3220 3220
Size: 5899
AclI AA'CG_TT
Cuts at: 1563 4026 4399 1563
Size: 2463 373 3063
Fragments arranged by size:
3063 2463 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3962 5346 5614 1632
Size: 2330 1384 268 1917
Fragments arranged by size:
2330 1917 1384 268
AfeI AGC'GCT
Cuts at: 2547 2547
Size: 5899
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5702
AflIII A'CryG_T
Cuts at: 1203 3120 4798 5775 1203
Size: 1917 1678 977 1327
Fragments arranged by size:
1917 1678 1327 977
AgeI A'CCGG_T
Cuts at: 4730 4730
Size: 5899
AhdI GACnn_n'nnGTC
Cuts at: 2512 4628 2512
Size: 2116 3783
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5899
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5899
AlwNI CAG_nnn'CTG
Cuts at: 3642 5214 3642
Size: 1572 4327
ApaI G_GGCC'C
Cuts at: 2096 2269 2440 3027 2096
Size: 173 171 587 4968
Fragments arranged by size:
4968 587 173 171
ApaLI G'TGCA_C
Cuts at: 2320 3956 5112 2320
Size: 1636 1156 3107
Fragments arranged by size:
3107 1636 1156
ApoI r'AATT_y
Cuts at: 1493 3208 3434 3586 5695 1493
Size: 1715 226 152 2109 1697
Fragments arranged by size:
2109 1715 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4453 160
Size: 872 3421 1606
Fragments arranged by size:
3421 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5899
AvaI C'yCGr_G
Cuts at: 1870 1943 2070 2084 2131 2285 2342 2488 2633
Size: 73 127 14 47 154 57 146 145
Cuts at: 2633 3129 3190 3224 1870
Size: 496 61 34 4545
Fragments arranged by size:
4545 496 154 146 145 127 73 61
57 47 34 14
BamHI G'GATC_C
Cuts at: 3229 3596 3229
Size: 367 5532
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2207 2684 2783 2845 3062
Size: 325 217 627 420 477 99 62 217
Cuts at: 3062 3091 3220 4675 618
Size: 29 129 1455 1842
Fragments arranged by size:
1842 1455 627 477 420 325 217 217
129 99 62 29
BanII G_rGCy'C
Cuts at: 1391 2096 2269 2349 2440 3027 3077 3218 1391
Size: 705 173 80 91 587 50 141 4072
Fragments arranged by size:
4072 705 587 173 141 91 80 50
BbeI G_GCGC'C
Cuts at: 2211 2688 2849 2211
Size: 477 161 5261
Fragments arranged by size:
5261 477 161
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2206 928
Size: 971 307 4621
Fragments arranged by size:
4621 971 307
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4090 4124 1903
Size: 34 2153 34 3678
Fragments arranged by size:
3678 2153 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3791 5001 3791
Size: 1210 4689
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5899
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2033 2045 2072
Size: 107 122 71 631 605 361 12 27
Cuts at: 2072 2154 2273 2435 2781 4510 136
Size: 82 119 162 346 1729 1525
Fragments arranged by size:
1729 1525 631 605 361 346 162 122
119 107 82 71 27 12
BglII A'GATC_T
Cuts at: 5894 5894
Size: 5899
BlpI GC'TnA_GC
Cuts at: 2400 2417 2717 2732 3316 2400
Size: 17 300 15 584 4983
Fragments arranged by size:
4983 584 300 17 15
Bme1580I G_kGCm'C
Cuts at: 1790 2096 2269 2324 2440 2646 3027 3960 5116
Size: 306 173 55 116 206 381 933 1156
Cuts at: 5116 1790
Size: 2573
Fragments arranged by size:
2573 1156 933 381 306 206 173 116
55
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5899
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2323 3630 4588
Size: 416 53 83 399 24 906 1307 958
Cuts at: 4588 442
Size: 1753
Fragments arranged by size:
1753 1307 958 906 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5899
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4559 1239
Size: 3320 2579
Bpu10I CC'TnA_GC
Cuts at: 2120 2120
Size: 5899
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4024 4889 5187 4024
Size: 865 298 4736
Fragments arranged by size:
4736 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4562 882
Size: 617 3063 2219
Fragments arranged by size:
3063 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2257 3121 3690 493
Size: 711 1053 864 569 2702
Fragments arranged by size:
2702 1053 864 711 569
BsaBI GATnn'nnATC
Cuts at: 5893 5893
Size: 5899
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2208 2374 2685 2846
Size: 53 83 186 936 675 166 311 161
Cuts at: 2846 2991 3706 4088 4723 275
Size: 145 715 382 635 1451
Fragments arranged by size:
1451 936 715 675 635 382 311 186
166 161 145 83 53
BsaWI w'CCGG_w
Cuts at: 3077 4332 4730 5004 5151 5562 3077
Size: 1255 398 274 147 411 3414
Fragments arranged by size:
3414 1255 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3072 3102 1178
Size: 30 165 30 1669 30 3975
Fragments arranged by size:
3975 1669 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2540 2603 2612 2615 1186
Size: 1354 63 9 3 4470
Fragments arranged by size:
4470 1354 63 9 3
BseYI C'CCAG_C
Cuts at: 2548 2566 5102 5598 2548
Size: 18 2536 496 2849
Fragments arranged by size:
2849 2536 496 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2237 2279 1163
Size: 573 501 42 4783
Fragments arranged by size:
4783 573 501 42
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2040 2205 2277 3349 4110 5138
Size: 275 186 39 165 72 1072 761 1028
Cuts at: 5138 1540
Size: 2301
Fragments arranged by size:
2301 1072 1028 761 275 186 165 72
39
BsiHKAI G_wGCw'C
Cuts at: 2324 2733 3960 4045 5116 2324
Size: 409 1227 85 1071 3107
Fragments arranged by size:
3107 1227 1071 409 85
BsmI GAATG_Cn'
Cuts at: 3410 3503 3410
Size: 93 5806
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2096 2269 2324 2349 2440 2646 2733
Size: 399 306 173 55 25 91 206 87
Cuts at: 2733 3027 3077 3218 3960 4045 5116 1391
Size: 294 50 141 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 306 294 206 173
141 91 87 85 55 50 25
BspEI T'CCGG_A
Cuts at: 3077 5562 3077
Size: 2485 3414
BspHI T'CATG_A
Cuts at: 3788 5518 3788
Size: 1730 4169
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2253 2365 3254 844
Size: 1409 112 889 3489
Fragments arranged by size:
3489 1409 889 112
BsrBI CCG'CTC
Cuts at: 1394 1823 2302 2851 3786 1394
Size: 429 479 549 935 3507
Fragments arranged by size:
3507 935 549 479 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2657 4394 4568 65
Size: 1183 1409 1737 174 1396
Fragments arranged by size:
1737 1409 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2204 2744 2864 4543 4730 1700
Size: 110 394 540 120 1679 187 2869
Fragments arranged by size:
2869 1679 540 394 187 120 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5899
BssHII G'CGCG_C
Cuts at: 1925 2447 1925
Size: 522 5377
BssSI C'ACGA_G
Cuts at: 3959 4971 3959
Size: 1012 4887
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5899
BstBI TT'CG_AA
Cuts at: 4703 4703
Size: 5899
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5899
BstXI CCAn_nnnn'nTGG
Cuts at: 3148 3148
Size: 5899
BstYI r'GATC_y
Cuts at: 1972 3229 3596 3981 3998 5450 5536 5548 5894
Size: 1257 367 385 17 1452 86 12 346
Cuts at: 5894 1972
Size: 1977
Fragments arranged by size:
1977 1452 1257 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2096 2149 2199 2277 2368 2616 2817
Size: 552 1031 53 50 78 91 248 201
Cuts at: 2817 2996 3039 513
Size: 179 43 3373
Fragments arranged by size:
3373 1031 552 248 201 179 91 78
53 50 43
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5160
Fragments arranged by size:
5160 683 56
BtsI GCAGTG_nn'
Cuts at: 2021 2267 2750 3051 3415 4209 4229 2021
Size: 246 483 301 364 794 20 3691
Fragments arranged by size:
3691 794 483 364 301 246 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5899
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5864
DraI TTT'AAA
Cuts at: 3203 3203
Size: 5899
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4906 809
Size: 507 3590 1802
Fragments arranged by size:
3590 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2037 2202 2274
Size: 54 1425 50 275 18 207 165 72
Cuts at: 2274 2742 2964 3346 4235 5864 8
Size: 468 222 382 889 1629 43
Fragments arranged by size:
1629 1425 889 468 382 275 222 207
165 72 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2037 2202 2274 3346 1537
Size: 275 225 165 72 1072 4090
Fragments arranged by size:
4090 1072 275 225 165 72
EarI CTCTTCn'nnn_
Cuts at: 1353 2459 3829 1353
Size: 1106 1370 3423
Fragments arranged by size:
3423 1370 1106
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2546 4486 4860 5006 387
Size: 1064 138 957 1940 374 146 1280
Fragments arranged by size:
1940 1280 1064 957 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3962 4559 5346 5614 1239
Size: 393 2330 597 787 268 1524
Fragments arranged by size:
2330 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2266 2282 3024 3145 2266
Size: 16 742 121 5020
Fragments arranged by size:
5020 742 121 16
EcoRI G'AATT_C
Cuts at: 3208 3586 3208
Size: 378 5521
FseI GG_CCGG'CC
Cuts at: 2748 2748
Size: 5899
FspI TGC'GCA
Cuts at: 4405 4405
Size: 5899
HaeII r_GCGC'y
Cuts at: 1678 2067 2211 2485 2549 2688 2828 2837 2849
Size: 389 144 274 64 139 140 9 12
Cuts at: 2849 5046 1678
Size: 2197 2531
Fragments arranged by size:
2531 2197 389 274 144 140 139 64
12 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2498 2530 4540 4572 4614
Size: 32 135 32 926 32 2010 32 42
Cuts at: 4614 4646 1373
Size: 32 2626
Fragments arranged by size:
2626 2010 926 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3243 3489 669
Size: 988 281 1305 246 3079
Fragments arranged by size:
3079 1305 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5899
HpaI GTT'AAC
Cuts at: 3489 3489
Size: 5899
KasI G'GCGC_C
Cuts at: 2207 2684 2845 2207
Size: 477 161 5261
Fragments arranged by size:
5261 477 161
KpnI G_GTAC'C
Cuts at: 3224 3224
Size: 5899
MfeI C'AATT_G
Cuts at: 3498 3498
Size: 5899
MluI A'CGCG_T
Cuts at: 5775 5775
Size: 5899
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2496 2837 5013 5197 1305
Size: 1191 341 2176 184 2007
Fragments arranged by size:
2176 2007 1191 341 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4077
Fragments arranged by size:
4077 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2621 3857 4216 4375 518
Size: 648 1455 1236 359 159 2042
Fragments arranged by size:
2042 1455 1236 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2109 2201 2279 2370 2471 2819 2957 3041
Size: 503 92 78 91 101 348 138 84
Cuts at: 3041 3315 3992 5140 5385 1606
Size: 274 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 503 348 274 245 138
101 92 91 84 78
NaeI GCC'GGC
Cuts at: 2206 2746 2866 2206
Size: 540 120 5239
Fragments arranged by size:
5239 540 120
NarI GG'CG_CC
Cuts at: 2208 2685 2846 2208
Size: 477 161 5261
Fragments arranged by size:
5261 477 161
NcoI C'CATG_G
Cuts at: 513 1065 2616 513
Size: 552 1551 3796
Fragments arranged by size:
3796 1551 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5899
NgoMIV G'CCGG_C
Cuts at: 2204 2744 2864 2204
Size: 540 120 5239
Fragments arranged by size:
5239 540 120
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5899
NotI GC'GGCC_GC
Cuts at: 3346 3346
Size: 5899
NspI r_CATG'y
Cuts at: 1132 1229 3257 4802 1132
Size: 97 2028 1545 2229
Fragments arranged by size:
2229 2028 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2312 1348
Size: 964 4935
PciI A'CATG_T
Cuts at: 4798 4798
Size: 5899
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5899
PmeI GTTT'AAAC
Cuts at: 3203 3203
Size: 5899
PmlI CAC'GTG
Cuts at: 2257 3121 2257
Size: 864 5035
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3968 4000 5481 5513 3968
Size: 32 1481 32 4354
Fragments arranged by size:
4354 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2282 3145 2282
Size: 863 5036
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5899
PsiI TTA'TAA
Cuts at: 3469 3469
Size: 5899
PspOMI G'GGCC_C
Cuts at: 2092 2265 2436 3023 2092
Size: 173 171 587 4968
Fragments arranged by size:
4968 587 173 171
PspXI vC'TCGA_Gb
Cuts at: 2342 2342
Size: 5899
PstI C_TGCA'G
Cuts at: 830 2108 2385 3251 830
Size: 1278 277 866 3478
Fragments arranged by size:
3478 1278 866 277
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5899
SacII CC_GC'GG
Cuts at: 2202 2280 2371 2820 3042 2202
Size: 78 91 449 222 5059
Fragments arranged by size:
5059 449 222 91 78
SalI G'TCGA_C
Cuts at: 1936 3241 1936
Size: 1305 4594
SanDI GG'GwC_CC
Cuts at: 2282 2282
Size: 5899
SbfI CC_TGCA'GG
Cuts at: 3251 3251
Size: 5899
ScaI AGT'ACT
Cuts at: 4147 5859 4147
Size: 1712 4187
SfcI C'TryA_G
Cuts at: 826 1045 2104 2381 3247 4382 5063 5254 826
Size: 219 1059 277 866 1135 681 191 1471
Fragments arranged by size:
1471 1135 1059 866 681 277 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2273 2435 2273
Size: 162 5737
SfoI GGC'GCC
Cuts at: 2209 2686 2847 2209
Size: 477 161 5261
Fragments arranged by size:
5261 477 161
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5899
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5899
SmaI CCC'GGG
Cuts at: 1872 2072 2133 2287 3192 3226 1872
Size: 200 61 154 905 34 4545
Fragments arranged by size:
4545 905 200 154 61 34
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2342 2488 4003 4904 5166 820
Size: 197 926 399 146 1515 901 262 1553
Fragments arranged by size:
1553 1515 926 901 399 262 197 146
SnaBI TAC'GTA
Cuts at: 493 3690 493
Size: 3197 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5899
SphI G_CATG'C
Cuts at: 1132 3257 1132
Size: 2125 3774
SrfI GCCC'GGGC
Cuts at: 2133 2133
Size: 5899
SspI AAT'ATT
Cuts at: 5 52 3823 5
Size: 47 3771 2081
Fragments arranged by size:
3771 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5899
StyI C'CwwG_G
Cuts at: 513 1065 2616 2646 3338 513
Size: 552 1551 30 692 3074
Fragments arranged by size:
3074 1551 692 552 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2863 3926 3943 4096 4281 4726 325
Size: 1255 1283 1063 17 153 185 445 1498
Fragments arranged by size:
1498 1283 1255 1063 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4145 5857 96
Size: 274 80 33 51 1443 2168 1712 138
Fragments arranged by size:
2168 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3072 3104 5859 5891 3072
Size: 32 2755 32 3080
Fragments arranged by size:
3080 2755 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5899
XbaI T'CTAG_A
Cuts at: 3235 3235
Size: 5899
XhoI C'TCGA_G
Cuts at: 1943 2342 2488 1943
Size: 399 146 5354
Fragments arranged by size:
5354 399 146
XmaI C'CCGG_G
Cuts at: 1870 2070 2131 2285 3190 3224 1870
Size: 200 61 154 905 34 4545
Fragments arranged by size:
4545 905 200 154 61 34
XmnI GAAnn'nnTTC
Cuts at: 4028 4028
Size: 5899
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3707 276
Size: 53 83 186 936 2173 2468
Fragments arranged by size:
2468 2173 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FseI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI SacII SalI SanDI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI
FspAI NruI NsiI PacI PfoI PsrI PvuII RsrII
SacI SapI SexAI SwaI XcmI