Restriction map for
pFN21AE6688
(Circular) (Six-base) MAPSORT of: pFN21AE6688.seq Check: 4696 from: 1 to: 5410
pFN21AE6688 5410 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3220 278
Size: 53 83 186 936 1684 2468
Fragments arranged by size:
2468 1684 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2753 1937
Size: 816 4594
Acc65I G'GTAC_C
Cuts at: 2731 2731
Size: 5410
AclI AA'CG_TT
Cuts at: 1563 3537 3910 1563
Size: 1974 373 3063
Fragments arranged by size:
3063 1974 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3473 4857 5125 1632
Size: 1841 1384 268 1917
Fragments arranged by size:
1917 1841 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5213
AflIII A'CryG_T
Cuts at: 1203 4309 5286 1203
Size: 3106 977 1327
Fragments arranged by size:
3106 1327 977
AgeI A'CCGG_T
Cuts at: 4241 4241
Size: 5410
AhdI GACnn_n'nnGTC
Cuts at: 4139 4139
Size: 5410
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5410
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5410
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2114 2146 2114
Size: 32 5378
AlwNI CAG_nnn'CTG
Cuts at: 3153 4725 3153
Size: 1572 3838
ApaI G_GGCC'C
Cuts at: 2081 2394 2478 2081
Size: 313 84 5013
Fragments arranged by size:
5013 313 84
ApaLI G'TGCA_C
Cuts at: 3467 4623 3467
Size: 1156 4254
ApoI r'AATT_y
Cuts at: 1493 2719 2945 3097 5206 1493
Size: 1226 226 152 2109 1697
Fragments arranged by size:
2109 1697 1226 226 152
AscI GG'CGCG_CC
Cuts at: 2016 2016
Size: 5410
AseI AT'TA_AT
Cuts at: 160 1032 3964 160
Size: 872 2932 1606
Fragments arranged by size:
2932 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5410
AvaI C'yCGr_G
Cuts at: 1870 1943 2087 2093 2687 2735 1870
Size: 73 144 6 594 48 4545
Fragments arranged by size:
4545 594 144 73 48 6
BamHI G'GATC_C
Cuts at: 2740 3107 2740
Size: 367 5043
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2450 2512 2619 2731 4186
Size: 325 217 627 663 62 107 112 1455
Cuts at: 4186 618
Size: 1842
Fragments arranged by size:
1842 1455 663 627 325 217 112 107
62
BanII G_rGCy'C
Cuts at: 1391 2081 2094 2394 2478 2729 1391
Size: 690 13 300 84 251 4072
Fragments arranged by size:
4072 690 300 251 84 13
BbeI G_GCGC'C
Cuts at: 2454 2623 2454
Size: 169 5241
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4439
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2042 2076 3601 3635 1903
Size: 34 50 34 21 34 1525 34 3678
Fragments arranged by size:
3678 1525 50 34 34 34 34 21
BciVI GTATCCnnnnn_n'
Cuts at: 3302 4512 3302
Size: 1210 4200
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5410
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2389 2473 2659
Size: 107 122 71 631 605 717 84 186
Cuts at: 2659 4021 136
Size: 1362 1525
Fragments arranged by size:
1525 1362 717 631 605 186 122 107
84 71
BglII A'GATC_T
Cuts at: 5405 5405
Size: 5410
BlpI GC'TnA_GC
Cuts at: 2827 2827
Size: 5410
Bme1580I G_kGCm'C
Cuts at: 1790 2081 2394 2478 2543 3471 4627 1790
Size: 291 313 84 65 928 1156 2573
Fragments arranged by size:
2573 1156 928 313 291 84 65
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5410
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2562 3141 4099
Size: 416 53 83 399 24 1145 579 958
Cuts at: 4099 442
Size: 1753
Fragments arranged by size:
1753 1145 958 579 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5410
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2426 4070 1239
Size: 1187 1644 2579
Fragments arranged by size:
2579 1644 1187
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3535 4400 4698 3535
Size: 865 298 4247
Fragments arranged by size:
4247 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4073 882
Size: 617 2574 2219
Fragments arranged by size:
2574 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3201 493
Size: 711 1997 2702
Fragments arranged by size:
2702 1997 711
BsaBI GATnn'nnATC
Cuts at: 5404 5404
Size: 5410
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2451 2496 2620 3217
Size: 53 83 186 936 918 45 124 597
Cuts at: 3217 3599 4234 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 918 635 597 382 186 124
83 53 45
BsaWI w'CCGG_w
Cuts at: 3843 4241 4515 4662 5073 3843
Size: 398 274 147 411 4180
Fragments arranged by size:
4180 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5185
Fragments arranged by size:
5185 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2168 1186
Size: 982 4428
BseYI C'CCAG_C
Cuts at: 2113 2477 4613 5109 2113
Size: 364 2136 496 2414
Fragments arranged by size:
2414 2136 496 364
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4837
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2183 2424 2860 3621 4649 1540
Size: 275 186 182 241 436 761 1028 2301
Fragments arranged by size:
2301 1028 761 436 275 241 186 182
BsiHKAI G_wGCw'C
Cuts at: 3471 3556 4627 3471
Size: 85 1071 4254
Fragments arranged by size:
4254 1071 85
BsmI GAATG_Cn'
Cuts at: 2921 3014 2921
Size: 93 5317
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2081 2094 2394 2478 2543 2729 3471
Size: 399 291 13 300 84 65 186 742
Cuts at: 3471 3556 4627 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 300 291 186 85
84 65 13
BspEI T'CCGG_A
Cuts at: 5073 5073
Size: 5410
BspHI T'CATG_A
Cuts at: 3299 5029 3299
Size: 1730 3680
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2682 2765 844
Size: 1838 83 3489
Fragments arranged by size:
3489 1838 83
BsrBI CCG'CTC
Cuts at: 1394 1823 3297 1394
Size: 429 1474 3507
Fragments arranged by size:
3507 1474 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3905 4079 65
Size: 1183 2657 174 1396
Fragments arranged by size:
2657 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2158 2182 4054 4241 1700
Size: 110 348 24 1872 187 2869
Fragments arranged by size:
2869 1872 348 187 110 24
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5410
BssHII G'CGCG_C
Cuts at: 1925 2016 2173 2175 2368 2426 2486 1925
Size: 91 157 2 193 58 60 4849
Fragments arranged by size:
4849 193 157 91 60 58 2
BssSI C'ACGA_G
Cuts at: 2536 3470 4482 2536
Size: 934 1012 3464
Fragments arranged by size:
3464 1012 934
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5410
BstBI TT'CG_AA
Cuts at: 4214 4214
Size: 5410
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5410
BstXI CCAn_nnnn'nTGG
Cuts at: 2575 2575
Size: 5410
BstYI r'GATC_y
Cuts at: 1972 2740 3107 3492 3509 4961 5047 5059 5405
Size: 768 367 385 17 1452 86 12 346
Cuts at: 5405 1972
Size: 1977
Fragments arranged by size:
1977 1452 768 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2386 2413 2663 513
Size: 552 1321 27 250 3260
Fragments arranged by size:
3260 1321 552 250 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2329 487
Size: 683 56 1103 3568
Fragments arranged by size:
3568 1103 683 56
BtsI GCAGTG_nn'
Cuts at: 2926 3720 3740 2926
Size: 794 20 4596
Fragments arranged by size:
4596 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5410
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5375
DraI TTT'AAA
Cuts at: 2714 2714
Size: 5410
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4417 809
Size: 507 3101 1802
Fragments arranged by size:
3101 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2180 2208 2421
Size: 54 1425 50 275 18 350 28 213
Cuts at: 2421 2577 2660 2857 3746 5375 8
Size: 156 83 197 889 1629 43
Fragments arranged by size:
1629 1425 889 350 275 213 197 156
83 54 50 43 28 18
EagI C'GGCC_G
Cuts at: 1537 1812 2180 2421 2857 1537
Size: 275 368 241 436 4090
Fragments arranged by size:
4090 436 368 275 241
EarI CTCTTCn'nnn_
Cuts at: 1353 3340 1353
Size: 1987 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2165 2671 3997 4371 4517 387
Size: 1064 138 576 506 1326 374 146 1280
Fragments arranged by size:
1326 1280 1064 576 506 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2426 3473 4070 4857 5125 1239
Size: 393 794 1047 597 787 268 1524
Fragments arranged by size:
1524 1047 794 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2597 2597
Size: 5410
EcoO109I rG'GnC_Cy
Cuts at: 2077 2078 2391 2474 2077
Size: 1 313 83 5013
Fragments arranged by size:
5013 313 83 1
EcoRI G'AATT_C
Cuts at: 2719 3097 2719
Size: 378 5032
FspI TGC'GCA
Cuts at: 2327 3916 2327
Size: 1589 3821
HaeII r_GCGC'y
Cuts at: 1678 2130 2279 2454 2623 4557 1678
Size: 452 149 175 169 1934 2531
Fragments arranged by size:
2531 1934 452 175 169 149
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2016 2048 2683 2715 4051
Size: 32 135 32 444 32 635 32 1336
Cuts at: 4051 4083 4125 4157 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1336 635 444 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2754 3000 669
Size: 988 281 816 246 3079
Fragments arranged by size:
3079 988 816 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5410
HpaI GTT'AAC
Cuts at: 3000 3000
Size: 5410
KasI G'GCGC_C
Cuts at: 2450 2619 2450
Size: 169 5241
KpnI G_GTAC'C
Cuts at: 2735 2735
Size: 5410
MfeI C'AATT_G
Cuts at: 3009 3009
Size: 5410
MluI A'CGCG_T
Cuts at: 5286 5286
Size: 5410
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4524 4708 1305
Size: 3219 184 2007
Fragments arranged by size:
3219 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3588
Fragments arranged by size:
3588 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3368 3727 3886 518
Size: 648 2202 359 159 2042
Fragments arranged by size:
2202 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2388 2529 2625 2665 2826 3503 4651 4896
Size: 782 141 96 40 161 677 1148 245
Cuts at: 4896 1606
Size: 2120
Fragments arranged by size:
2120 1148 782 677 245 161 141 96
40
NaeI GCC'GGC
Cuts at: 2160 2160
Size: 5410
NarI GG'CG_CC
Cuts at: 2451 2620 2451
Size: 169 5241
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4858
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5410
NgoMIV G'CCGG_C
Cuts at: 2158 2158
Size: 5410
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5410
NotI GC'GGCC_GC
Cuts at: 2857 2857
Size: 5410
NspI r_CATG'y
Cuts at: 1132 1229 2768 4313 1132
Size: 97 1539 1545 2229
Fragments arranged by size:
2229 1545 1539 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5410
PciI A'CATG_T
Cuts at: 4309 4309
Size: 5410
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5410
PfoI T'CCnGG_A
Cuts at: 2238 2238
Size: 5410
PmeI GTTT'AAAC
Cuts at: 2714 2714
Size: 5410
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3479 3511 4992 5024 3479
Size: 32 1481 32 3865
Fragments arranged by size:
3865 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5410
PsiI TTA'TAA
Cuts at: 2980 2980
Size: 5410
PspOMI G'GGCC_C
Cuts at: 2077 2390 2474 2077
Size: 313 84 5013
Fragments arranged by size:
5013 313 84
PstI C_TGCA'G
Cuts at: 830 2138 2473 2762 830
Size: 1308 335 289 3478
Fragments arranged by size:
3478 1308 335 289
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5410
SacII CC_GC'GG
Cuts at: 2389 2666 2389
Size: 277 5133
SalI G'TCGA_C
Cuts at: 1936 2752 1936
Size: 816 4594
SbfI CC_TGCA'GG
Cuts at: 2473 2762 2473
Size: 289 5121
ScaI AGT'ACT
Cuts at: 3658 5370 3658
Size: 1712 3698
SfcI C'TryA_G
Cuts at: 826 1045 2134 2469 2758 3893 4574 4765 826
Size: 219 1089 335 289 1135 681 191 1471
Fragments arranged by size:
1471 1135 1089 681 335 289 219 191
SfoI GGC'GCC
Cuts at: 2452 2621 2452
Size: 169 5241
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5410
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5410
SmaI CCC'GGG
Cuts at: 1872 2095 2689 2737 1872
Size: 223 594 48 4545
Fragments arranged by size:
4545 594 223 48
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3514 4415 4677 820
Size: 197 926 1571 901 262 1553
Fragments arranged by size:
1571 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3201 493
Size: 2708 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5410
SphI G_CATG'C
Cuts at: 1132 2768 1132
Size: 1636 3774
SspI AAT'ATT
Cuts at: 5 52 3334 5
Size: 47 3282 2081
Fragments arranged by size:
3282 2081 47
StuI AGG'CCT
Cuts at: 1517 2031 2255 2603 1517
Size: 514 224 348 4324
Fragments arranged by size:
4324 514 348 224
StyI C'CwwG_G
Cuts at: 513 1065 2193 2849 513
Size: 552 1128 656 3074
Fragments arranged by size:
3074 1128 656 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3437 3454 3607 3792 4237 325
Size: 1255 1857 17 153 185 445 1498
Fragments arranged by size:
1857 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3656 5368 96
Size: 274 80 33 51 1443 1679 1712 138
Fragments arranged by size:
1712 1679 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5370 5402 5370
Size: 32 5378
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5410
XbaI T'CTAG_A
Cuts at: 2746 2746
Size: 5410
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5410
XmaI C'CCGG_G
Cuts at: 1870 2093 2687 2735 1870
Size: 223 594 48 4545
Fragments arranged by size:
4545 594 223 48
XmnI GAAnn'nnTTC
Cuts at: 3539 3539
Size: 5410
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3218 276
Size: 53 83 186 936 1684 2468
Fragments arranged by size:
2468 1684 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AvrII BaeI BbvCI BfrBI BplI Bpu10I
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI
FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI
PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI
SrfI SwaI XcmI