Restriction map for
pFN21AE6687
(Circular) (Six-base) MAPSORT of: pFN21AE6687.seq Check: 6686 from: 1 to: 5401
pFN21AE6687 5401 bp
With 182 enzymes: SgfI *
August 29, 2014 11:22 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3211 278
Size: 53 83 186 936 1675 2468
Fragments arranged by size:
2468 1675 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2744 1937
Size: 807 4594
Acc65I G'GTAC_C
Cuts at: 2722 2722
Size: 5401
AclI AA'CG_TT
Cuts at: 1563 3528 3901 1563
Size: 1965 373 3063
Fragments arranged by size:
3063 1965 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2524 3464 4848 5116 1632
Size: 892 940 1384 268 1917
Fragments arranged by size:
1917 1384 940 892 268
AfeI AGC'GCT
Cuts at: 2012 2549 2645 2012
Size: 537 96 4768
Fragments arranged by size:
4768 537 96
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5204
AflIII A'CryG_T
Cuts at: 1203 2481 4300 5277 1203
Size: 1278 1819 977 1327
Fragments arranged by size:
1819 1327 1278 977
AgeI A'CCGG_T
Cuts at: 4232 4232
Size: 5401
AhdI GACnn_n'nnGTC
Cuts at: 4130 4130
Size: 5401
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5401
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2441 1184
Size: 1257 4144
AlwNI CAG_nnn'CTG
Cuts at: 2326 3144 4716 2326
Size: 818 1572 3011
Fragments arranged by size:
3011 1572 818
ApaLI G'TGCA_C
Cuts at: 2597 3458 4614 2597
Size: 861 1156 3384
Fragments arranged by size:
3384 1156 861
ApoI r'AATT_y
Cuts at: 1493 2710 2936 3088 5197 1493
Size: 1217 226 152 2109 1697
Fragments arranged by size:
2109 1697 1217 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3955 160
Size: 872 2923 1606
Fragments arranged by size:
2923 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5401
AvaI C'yCGr_G
Cuts at: 1870 1943 2213 2255 2564 2726 1870
Size: 73 270 42 309 162 4545
Fragments arranged by size:
4545 309 270 162 73 42
AvrII C'CTAG_G
Cuts at: 2023 2023
Size: 5401
BamHI G'GATC_C
Cuts at: 2731 3098 2731
Size: 367 5034
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2110 2722 4177 618
Size: 325 217 627 323 612 1455 1842
Fragments arranged by size:
1842 1455 627 612 325 323 217
BanII G_rGCy'C
Cuts at: 1391 2720 1391
Size: 1329 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4430
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3592 3626 1903
Size: 34 1655 34 3678
Fragments arranged by size:
3678 1655 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3293 4503 3293
Size: 1210 4191
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5401
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4012 136
Size: 107 122 71 631 605 2340 1525
Fragments arranged by size:
2340 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5396 5396
Size: 5401
BlpI GC'TnA_GC
Cuts at: 2818 2818
Size: 5401
Bme1580I G_kGCm'C
Cuts at: 1790 2565 2601 3462 4618 1790
Size: 775 36 861 1156 2573
Fragments arranged by size:
2573 1156 861 775 36
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5401
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3132 4090 442
Size: 416 53 83 399 24 1715 958 1753
Fragments arranged by size:
1753 1715 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5401
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4061 1239
Size: 2822 2579
Bpu10I CC'TnA_GC
Cuts at: 2414 2414
Size: 5401
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3526 4391 4689 3526
Size: 865 298 4238
Fragments arranged by size:
4238 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4064 882
Size: 617 2565 2219
Fragments arranged by size:
2565 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2521 3192 493
Size: 711 1317 671 2702
Fragments arranged by size:
2702 1317 711 671
BsaBI GATnn'nnATC
Cuts at: 2660 5395 2660
Size: 2735 2666
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3208 3590 4225 275
Size: 53 83 186 936 1675 382 635 1451
Fragments arranged by size:
1675 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3834 4232 4506 4653 5064 3834
Size: 398 274 147 411 4171
Fragments arranged by size:
4171 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5176
Fragments arranged by size:
5176 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5401
BseYI C'CCAG_C
Cuts at: 2018 2114 2288 4604 5100 2018
Size: 96 174 2316 496 2319
Fragments arranged by size:
2319 2316 496 174 96
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2304 1163
Size: 573 568 4260
Fragments arranged by size:
4260 573 568
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2079 2339 2851 3612 4640 1540
Size: 275 186 78 260 512 761 1028 2301
Fragments arranged by size:
2301 1028 761 512 275 260 186 78
BsiHKAI G_wGCw'C
Cuts at: 2558 2601 2696 3462 3547 4618 2558
Size: 43 95 766 85 1071 3341
Fragments arranged by size:
3341 1071 766 95 85 43
BsmI GAATG_Cn'
Cuts at: 2912 3005 2912
Size: 93 5308
BsmBI CGTCTCn'nnnn_
Cuts at: 2186 2186
Size: 5401
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2558 2565 2601 2696 2720 3462 3547
Size: 399 768 7 36 95 24 742 85
Cuts at: 3547 4618 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 768 742 399 95 85 36
24 7
BspEI T'CCGG_A
Cuts at: 5064 5064
Size: 5401
BspHI T'CATG_A
Cuts at: 3290 5020 3290
Size: 1730 3671
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2133 2756 844
Size: 1289 623 3489
Fragments arranged by size:
3489 1289 623
BsrBI CCG'CTC
Cuts at: 1394 1823 3288 1394
Size: 429 1465 3507
Fragments arranged by size:
3507 1465 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3896 4070 65
Size: 1183 2648 174 1396
Fragments arranged by size:
2648 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2074 2078 2262 4045 4232 1700
Size: 110 264 4 184 1783 187 2869
Fragments arranged by size:
2869 1783 264 187 184 110 4
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5401
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5401
BssSI C'ACGA_G
Cuts at: 2551 2695 3461 4473 2551
Size: 144 766 1012 3479
Fragments arranged by size:
3479 1012 766 144
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2577 794
Size: 1783 3618
BstBI TT'CG_AA
Cuts at: 4205 4205
Size: 5401
BstEII G'GTnAC_C
Cuts at: 1184 2029 1184
Size: 845 4556
BstYI r'GATC_y
Cuts at: 1972 2182 2731 3098 3483 3500 4952 5038 5050
Size: 210 549 367 385 17 1452 86 12
Cuts at: 5050 5396 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 549 385 367 346 210 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3910
Fragments arranged by size:
3910 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4662
Fragments arranged by size:
4662 683 56
BtsI GCAGTG_nn'
Cuts at: 2917 3711 3731 2917
Size: 794 20 4587
Fragments arranged by size:
4587 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5401
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5366
DraI TTT'AAA
Cuts at: 2705 2705
Size: 5401
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4408 809
Size: 507 3092 1802
Fragments arranged by size:
3092 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2076 2083 2241
Size: 54 1425 50 275 18 246 7 158
Cuts at: 2241 2336 2510 2848 3737 5366 8
Size: 95 174 338 889 1629 43
Fragments arranged by size:
1629 1425 889 338 275 246 174 158
95 54 50 43 18 7
EagI C'GGCC_G
Cuts at: 1537 1812 2076 2336 2848 1537
Size: 275 264 260 512 4090
Fragments arranged by size:
4090 512 275 264 260
EarI CTCTTCn'nnn_
Cuts at: 1353 3331 1353
Size: 1978 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2093 2324 3988 4362 4508 387
Size: 1064 138 504 231 1664 374 146 1280
Fragments arranged by size:
1664 1280 1064 504 374 231 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2524 3464 4061 4848 5116 1239
Size: 393 892 940 597 787 268 1524
Fragments arranged by size:
1524 940 892 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2302 2302
Size: 5401
EcoRI G'AATT_C
Cuts at: 2710 3088 2710
Size: 378 5023
FspI TGC'GCA
Cuts at: 3907 3907
Size: 5401
HaeII r_GCGC'y
Cuts at: 1678 2014 2413 2551 2623 2647 4548 1678
Size: 336 399 138 72 24 1901 2531
Fragments arranged by size:
2531 1901 399 336 138 72 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4042 4074 4116 4148 1373
Size: 32 135 32 2470 32 42 32 2626
Fragments arranged by size:
2626 2470 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2745 2991 669
Size: 988 281 807 246 3079
Fragments arranged by size:
3079 988 807 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5401
HpaI GTT'AAC
Cuts at: 2991 2991
Size: 5401
KpnI G_GTAC'C
Cuts at: 2726 2726
Size: 5401
MfeI C'AATT_G
Cuts at: 3000 3000
Size: 5401
MluI A'CGCG_T
Cuts at: 2481 5277 2481
Size: 2796 2605
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4515 4699 1305
Size: 3210 184 2007
Fragments arranged by size:
3210 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2243 10
Size: 54 1425 343 411 3168
Fragments arranged by size:
3168 1425 411 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3359 3718 3877 518
Size: 648 2193 359 159 2042
Fragments arranged by size:
2193 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2288 2323 2335 2535 2629 2817 3494 4642
Size: 682 35 12 200 94 188 677 1148
Cuts at: 4642 4887 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 682 677 245 200 188 94
35 12
NaeI GCC'GGC
Cuts at: 2080 2080
Size: 5401
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3910
Fragments arranged by size:
3910 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5401
NgoMIV G'CCGG_C
Cuts at: 2078 2078
Size: 5401
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5401
NotI GC'GGCC_GC
Cuts at: 2848 2848
Size: 5401
NspI r_CATG'y
Cuts at: 1132 1229 2759 4304 1132
Size: 97 1530 1545 2229
Fragments arranged by size:
2229 1545 1530 97
PasI CC'CwG_GG
Cuts at: 1348 2395 1348
Size: 1047 4354
PciI A'CATG_T
Cuts at: 4300 4300
Size: 5401
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5401
PmeI GTTT'AAAC
Cuts at: 2705 2705
Size: 5401
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3470 3502 4983 5015 3470
Size: 32 1481 32 3856
Fragments arranged by size:
3856 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2302 2302
Size: 5401
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5401
PsiI TTA'TAA
Cuts at: 2971 2971
Size: 5401
PstI C_TGCA'G
Cuts at: 830 2753 830
Size: 1923 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5401
PvuII CAG'CTG
Cuts at: 2288 2323 2535 2288
Size: 35 212 5154
Fragments arranged by size:
5154 212 35
SalI G'TCGA_C
Cuts at: 1936 2743 1936
Size: 807 4594
SbfI CC_TGCA'GG
Cuts at: 2753 2753
Size: 5401
ScaI AGT'ACT
Cuts at: 3649 5361 3649
Size: 1712 3689
SfcI C'TryA_G
Cuts at: 826 1045 2749 3884 4565 4756 826
Size: 219 1704 1135 681 191 1471
Fragments arranged by size:
1704 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5401
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5401
SmaI CCC'GGG
Cuts at: 1872 2728 1872
Size: 856 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3505 4406 4668 820
Size: 197 926 1562 901 262 1553
Fragments arranged by size:
1562 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3192 493
Size: 2699 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5401
SphI G_CATG'C
Cuts at: 1132 2759 1132
Size: 1627 3774
SspI AAT'ATT
Cuts at: 5 52 3325 5
Size: 47 3273 2081
Fragments arranged by size:
3273 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5401
StyI C'CwwG_G
Cuts at: 513 1065 2004 2023 2172 2840 513
Size: 552 939 19 149 668 3074
Fragments arranged by size:
3074 939 668 552 149 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2128 2166 3428 3445 3598 3783 4228
Size: 1255 548 38 1262 17 153 185 445
Cuts at: 4228 325
Size: 1498
Fragments arranged by size:
1498 1262 1255 548 445 185 153 38
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3647 5359 96
Size: 274 80 33 51 1443 1670 1712 138
Fragments arranged by size:
1712 1670 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5361 5393 5361
Size: 32 5369
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5401
XbaI T'CTAG_A
Cuts at: 2737 2737
Size: 5401
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5401
XmaI C'CCGG_G
Cuts at: 1870 2726 1870
Size: 856 4545
XmnI GAAnn'nnTTC
Cuts at: 3530 3530
Size: 5401
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3209 276
Size: 53 83 186 936 1675 2468
Fragments arranged by size:
2468 1675 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI BaeI BbeI BbvCI BfrBI
BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI