Restriction map for
pFN21AE6686
(Circular) (Six-base) MAPSORT of: pFN21AE6686.seq Check: 1236 from: 1 to: 5551
pFN21AE6686 5551 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3361 278
Size: 53 83 186 936 1825 2468
Fragments arranged by size:
2468 1825 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2894 1937
Size: 957 4594
Acc65I G'GTAC_C
Cuts at: 2872 2872
Size: 5551
AclI AA'CG_TT
Cuts at: 1563 3678 4051 1563
Size: 2115 373 3063
Fragments arranged by size:
3063 2115 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3614 4998 5266 1632
Size: 1982 1384 268 1917
Fragments arranged by size:
1982 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5354
AflIII A'CryG_T
Cuts at: 1203 4450 5427 1203
Size: 3247 977 1327
Fragments arranged by size:
3247 1327 977
AgeI A'CCGG_T
Cuts at: 4382 4382
Size: 5551
AhdI GACnn_n'nnGTC
Cuts at: 4280 4280
Size: 5551
AleI CACnn'nnGTG
Cuts at: 1166 2449 1166
Size: 1283 4268
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5551
AlwNI CAG_nnn'CTG
Cuts at: 2349 2708 3294 4866 2349
Size: 359 586 1572 3034
Fragments arranged by size:
3034 1572 586 359
ApaI G_GGCC'C
Cuts at: 2553 2607 2553
Size: 54 5497
ApaLI G'TGCA_C
Cuts at: 3608 4764 3608
Size: 1156 4395
ApoI r'AATT_y
Cuts at: 1493 2860 3086 3238 5347 1493
Size: 1367 226 152 2109 1697
Fragments arranged by size:
2109 1697 1367 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4105 160
Size: 872 3073 1606
Fragments arranged by size:
3073 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5551
AvaI C'yCGr_G
Cuts at: 1870 1943 2876 1870
Size: 73 933 4545
Fragments arranged by size:
4545 933 73
BamHI G'GATC_C
Cuts at: 2881 3248 2881
Size: 367 5184
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2872 4327 618
Size: 325 217 627 1085 1455 1842
Fragments arranged by size:
1842 1455 1085 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2053 2114 2415 2499 2553 2607 2770 2870
Size: 662 61 301 84 54 54 163 100
Cuts at: 2870 1391
Size: 4072
Fragments arranged by size:
4072 662 301 163 100 84 61 54
54
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2843 928
Size: 971 944 3636
Fragments arranged by size:
3636 971 944
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3742 3776 1903
Size: 34 1805 34 3678
Fragments arranged by size:
3678 1805 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3443 4653 3443
Size: 1210 4341
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5551
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2406 4162 136
Size: 107 122 71 631 605 734 1756 1525
Fragments arranged by size:
1756 1525 734 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5546 5546
Size: 5551
BlpI GC'TnA_GC
Cuts at: 2426 2968 2426
Size: 542 5009
Bme1580I G_kGCm'C
Cuts at: 1790 2168 2211 2553 2607 3612 4768 1790
Size: 378 43 342 54 1005 1156 2573
Fragments arranged by size:
2573 1156 1005 378 342 54 43
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5551
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2798 3282 4240
Size: 416 53 83 399 24 1381 484 958
Cuts at: 4240 442
Size: 1753
Fragments arranged by size:
1753 1381 958 484 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5551
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2117 2470 2585 2639 4211 1239
Size: 878 353 115 54 1572 2579
Fragments arranged by size:
2579 1572 878 353 115 54
Bpu10I CC'TnA_GC
Cuts at: 2155 2155
Size: 5551
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3676 4541 4839 3676
Size: 865 298 4388
Fragments arranged by size:
4388 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2658 4214 882
Size: 617 1159 1556 2219
Fragments arranged by size:
2219 1556 1159 617
BsaAI yAC'GTr
Cuts at: 493 1204 2451 3342 493
Size: 711 1247 891 2702
Fragments arranged by size:
2702 1247 891 711
BsaBI GATnn'nnATC
Cuts at: 5545 5545
Size: 5551
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2340 3358 3740 4375
Size: 53 83 186 936 807 1018 382 635
Cuts at: 4375 275
Size: 1451
Fragments arranged by size:
1451 1018 936 807 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3984 4382 4656 4803 5214 3984
Size: 398 274 147 411 4321
Fragments arranged by size:
4321 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5326
Fragments arranged by size:
5326 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2276 2279 2644 2735 1186
Size: 1090 3 365 91 4002
Fragments arranged by size:
4002 1090 365 91 3
BseYI C'CCAG_C
Cuts at: 2150 4754 5250 2150
Size: 2604 496 2451
Fragments arranged by size:
2604 2451 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2290 2317 1163
Size: 573 554 27 4397
Fragments arranged by size:
4397 573 554 27
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3001 3762 4790 1540
Size: 275 186 1000 761 1028 2301
Fragments arranged by size:
2301 1028 1000 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2415 2499 2718 3612 3697 4768 2415
Size: 84 219 894 85 1071 3198
Fragments arranged by size:
3198 1071 894 219 85 84
BsmI GAATG_Cn'
Cuts at: 2439 2593 2647 3062 3155 2439
Size: 154 54 415 93 4835
Fragments arranged by size:
4835 415 154 93 54
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2053 2114 2168 2211 2415 2499 2553
Size: 399 263 61 54 43 204 84 54
Cuts at: 2553 2607 2718 2770 2870 3612 3697 4768 1391
Size: 54 111 52 100 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 263 204 111 100
85 84 61 54 54 54 52 43
BspEI T'CCGG_A
Cuts at: 5214 5214
Size: 5551
BspHI T'CATG_A
Cuts at: 3440 5170 3440
Size: 1730 3821
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2017 2906 844
Size: 1173 889 3489
Fragments arranged by size:
3489 1173 889
BsrBI CCG'CTC
Cuts at: 1394 1823 2010 3438 1394
Size: 429 187 1428 3507
Fragments arranged by size:
3507 1428 429 187
BsrDI GCAATG_nn'
Cuts at: 65 1248 2306 4046 4220 65
Size: 1183 1058 1740 174 1396
Fragments arranged by size:
1740 1396 1183 1058 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2336 4195 4382 1700
Size: 110 526 1859 187 2869
Fragments arranged by size:
2869 1859 526 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5551
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5551
BssSI C'ACGA_G
Cuts at: 2161 3611 4623 2161
Size: 1450 1012 3089
Fragments arranged by size:
3089 1450 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2708 794
Size: 1914 3637
BstBI TT'CG_AA
Cuts at: 4355 4355
Size: 5551
BstEII G'GTnAC_C
Cuts at: 1184 2731 1184
Size: 1547 4004
BstXI CCAn_nnnn'nTGG
Cuts at: 2451 2545 2599 2451
Size: 94 54 5403
Fragments arranged by size:
5403 94 54
BstYI r'GATC_y
Cuts at: 1972 2517 2881 3248 3633 3650 5102 5188 5200
Size: 545 364 367 385 17 1452 86 12
Cuts at: 5200 5546 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 545 385 367 364 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2544 2598 513
Size: 552 1479 54 3466
Fragments arranged by size:
3466 1479 552 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2262 2271 2445 487
Size: 683 56 1036 9 174 3593
Fragments arranged by size:
3593 1036 683 174 56 9
BtsI GCAGTG_nn'
Cuts at: 3067 3861 3881 3067
Size: 794 20 4737
Fragments arranged by size:
4737 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5551
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5516
DraI TTT'AAA
Cuts at: 2855 2855
Size: 5551
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4558 809
Size: 507 3242 1802
Fragments arranged by size:
3242 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2464 2802 2998
Size: 54 1425 50 275 18 634 338 196
Cuts at: 2998 3887 5516 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 634 338 275 196 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2998 1537
Size: 275 1186 4090
Fragments arranged by size:
4090 1186 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3481 1353
Size: 2128 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2771 4138 4512 4658 387
Size: 1064 138 1182 1367 374 146 1280
Fragments arranged by size:
1367 1280 1182 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2413 2497 2413
Size: 84 5467
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2117 2470 2585 2639 3614 4211 4998
Size: 393 485 353 115 54 975 597 787
Cuts at: 4998 5266 1239
Size: 268 1524
Fragments arranged by size:
1524 975 787 597 485 393 353 268
115 54
EcoNI CCTnn'n_nnAGG
Cuts at: 2581 2581
Size: 5551
EcoO109I rG'GnC_Cy
Cuts at: 2147 2549 2550 2603 2604 2147
Size: 402 1 53 1 5094
Fragments arranged by size:
5094 402 53 1 1
EcoRI G'AATT_C
Cuts at: 2860 3238 2860
Size: 378 5173
FspI TGC'GCA
Cuts at: 2378 4057 2378
Size: 1679 3872
HaeII r_GCGC'y
Cuts at: 1678 2066 2224 2458 2779 4698 1678
Size: 388 158 234 321 1919 2531
Fragments arranged by size:
2531 1919 388 321 234 158
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4192 4224 4266 4298 1373
Size: 32 135 32 2620 32 42 32 2626
Fragments arranged by size:
2626 2620 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2895 3141 669
Size: 988 281 957 246 3079
Fragments arranged by size:
3079 988 957 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5551
HpaI GTT'AAC
Cuts at: 3141 3141
Size: 5551
KpnI G_GTAC'C
Cuts at: 2876 2876
Size: 5551
MfeI C'AATT_G
Cuts at: 3150 3150
Size: 5551
MluI A'CGCG_T
Cuts at: 5427 5427
Size: 5551
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1997 2011 4665 4849 1305
Size: 692 14 2654 184 2007
Fragments arranged by size:
2654 2007 692 184 14
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2804 10
Size: 54 1425 343 972 2757
Fragments arranged by size:
2757 1425 972 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2449 2543 2597 3509 3868 4027 518
Size: 648 1283 94 54 912 359 159 2042
Fragments arranged by size:
2042 1283 912 648 359 159 94 54
MspA1I CmG'CkG
Cuts at: 1606 2705 2967 3644 4792 5037 1606
Size: 1099 262 677 1148 245 2120
Fragments arranged by size:
2120 1148 1099 677 262 245
NcoI C'CATG_G
Cuts at: 513 1065 2544 2598 513
Size: 552 1479 54 3466
Fragments arranged by size:
3466 1479 552 54
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5551
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5551
NotI GC'GGCC_GC
Cuts at: 2998 2998
Size: 5551
NspI r_CATG'y
Cuts at: 1132 1229 2909 4454 1132
Size: 97 1680 1545 2229
Fragments arranged by size:
2229 1680 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5551
PciI A'CATG_T
Cuts at: 4450 4450
Size: 5551
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5551
PfoI T'CCnGG_A
Cuts at: 2523 2523
Size: 5551
PmeI GTTT'AAAC
Cuts at: 2855 2855
Size: 5551
PmlI CAC'GTG
Cuts at: 2451 2451
Size: 5551
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2035 2067 3620 3652 5133 5165 2035
Size: 32 1553 32 1481 32 2421
Fragments arranged by size:
2421 1553 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5551
PsiI TTA'TAA
Cuts at: 3121 3121
Size: 5551
PspOMI G'GGCC_C
Cuts at: 2549 2603 2549
Size: 54 5497
PstI C_TGCA'G
Cuts at: 830 2246 2387 2903 830
Size: 1416 141 516 3478
Fragments arranged by size:
3478 1416 516 141
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5551
PvuII CAG'CTG
Cuts at: 2705 2705
Size: 5551
SacI G_AGCT'C
Cuts at: 2415 2499 2415
Size: 84 5467
SalI G'TCGA_C
Cuts at: 1936 2893 1936
Size: 957 4594
SbfI CC_TGCA'GG
Cuts at: 2903 2903
Size: 5551
ScaI AGT'ACT
Cuts at: 3799 5511 3799
Size: 1712 3839
SfcI C'TryA_G
Cuts at: 826 1045 2242 2383 2899 4034 4715 4906 826
Size: 219 1197 141 516 1135 681 191 1471
Fragments arranged by size:
1471 1197 1135 681 516 219 191 141
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5551
SgrAI Cr'CCGG_yG
Cuts at: 1700 2336 1700
Size: 636 4915
SmaI CCC'GGG
Cuts at: 1872 2878 1872
Size: 1006 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3655 4556 4818 820
Size: 197 926 1712 901 262 1553
Fragments arranged by size:
1712 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3342 493
Size: 2849 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5551
SphI G_CATG'C
Cuts at: 1132 2909 1132
Size: 1777 3774
SspI AAT'ATT
Cuts at: 5 52 3475 5
Size: 47 3423 2081
Fragments arranged by size:
3423 2081 47
StuI AGG'CCT
Cuts at: 1517 2094 1517
Size: 577 4974
StyI C'CwwG_G
Cuts at: 513 1065 2544 2598 2990 513
Size: 552 1479 54 392 3074
Fragments arranged by size:
3074 1479 552 392 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2185 2484 3578 3595 3748 3933 4378
Size: 1255 605 299 1094 17 153 185 445
Cuts at: 4378 325
Size: 1498
Fragments arranged by size:
1498 1255 1094 605 445 299 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3797 5509 96
Size: 274 80 33 51 1443 1820 1712 138
Fragments arranged by size:
1820 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5511 5543 5511
Size: 32 5519
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5551
XbaI T'CTAG_A
Cuts at: 2887 2887
Size: 5551
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5551
XmaI C'CCGG_G
Cuts at: 1870 2876 1870
Size: 1006 4545
XmnI GAAnn'nnTTC
Cuts at: 3680 3680
Size: 5551
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3359 276
Size: 53 83 186 936 1825 2468
Fragments arranged by size:
2468 1825 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PpuMI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI