Restriction map for
pFN21AE6685
(Circular) (Six-base) MAPSORT of: pFN21AE6685.seq Check: 4243 from: 1 to: 5392
pFN21AE6685 5392 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3202 278
Size: 53 83 186 936 1666 2468
Fragments arranged by size:
2468 1666 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2400 2735 1937
Size: 463 335 4594
Fragments arranged by size:
4594 463 335
Acc65I G'GTAC_C
Cuts at: 2106 2713 2106
Size: 607 4785
AclI AA'CG_TT
Cuts at: 1563 3519 3892 1563
Size: 1956 373 3063
Fragments arranged by size:
3063 1956 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2291 2477 2495 3455 4839 5107 1632
Size: 659 186 18 960 1384 268 1917
Fragments arranged by size:
1917 1384 960 659 268 186 18
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5195
AflIII A'CryG_T
Cuts at: 1203 4291 5268 1203
Size: 3088 977 1327
Fragments arranged by size:
3088 1327 977
AgeI A'CCGG_T
Cuts at: 2103 4223 2103
Size: 2120 3272
AhdI GACnn_n'nnGTC
Cuts at: 4121 4121
Size: 5392
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5392
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2438 1184
Size: 1254 4138
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2198 2230 2198
Size: 32 5360
AlwNI CAG_nnn'CTG
Cuts at: 2040 2269 2503 3135 4707 2040
Size: 229 234 632 1572 2725
Fragments arranged by size:
2725 1572 632 234 229
ApaLI G'TGCA_C
Cuts at: 3449 4605 3449
Size: 1156 4236
ApoI r'AATT_y
Cuts at: 1493 2701 2927 3079 5188 1493
Size: 1208 226 152 2109 1697
Fragments arranged by size:
2109 1697 1208 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3946 160
Size: 872 2914 1606
Fragments arranged by size:
2914 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5392
AvaI C'yCGr_G
Cuts at: 1870 1943 2717 1870
Size: 73 774 4545
Fragments arranged by size:
4545 774 73
BamHI G'GATC_C
Cuts at: 2722 3089 2722
Size: 367 5025
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2106 2713 4168 618
Size: 325 217 627 319 607 1455 1842
Fragments arranged by size:
1842 1455 627 607 325 319 217
BanII G_rGCy'C
Cuts at: 1391 2153 2711 1391
Size: 762 558 4072
Fragments arranged by size:
4072 762 558
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4421
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2076 2110 3583 3617 1903
Size: 34 139 34 1473 34 3678
Fragments arranged by size:
3678 1473 139 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3284 4494 3284
Size: 1210 4182
BclI T'GATC_A
Cuts at: 1548 2508 1548
Size: 960 4432
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2015 4003 136
Size: 107 122 71 631 605 343 1988 1525
Fragments arranged by size:
1988 1525 631 605 343 122 107 71
BglII A'GATC_T
Cuts at: 5387 5387
Size: 5392
BlpI GC'TnA_GC
Cuts at: 2073 2809 2073
Size: 736 4656
Bme1580I G_kGCm'C
Cuts at: 1790 2034 3453 4609 1790
Size: 244 1419 1156 2573
Fragments arranged by size:
2573 1419 1156 244
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5392
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3123 4081 442
Size: 416 53 83 399 24 1706 958 1753
Fragments arranged by size:
1753 1706 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5392
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4052 1239
Size: 2813 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3517 4382 4680 3517
Size: 865 298 4229
Fragments arranged by size:
4229 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4055 882
Size: 617 2556 2219
Fragments arranged by size:
2556 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3183 493
Size: 711 1979 2702
Fragments arranged by size:
2702 1979 711
BsaBI GATnn'nnATC
Cuts at: 5386 5386
Size: 5392
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3199 3581 4216 275
Size: 53 83 186 936 1666 382 635 1451
Fragments arranged by size:
1666 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2103 2109 2340 3825 4223 4497 4644 5055 2103
Size: 6 231 1485 398 274 147 411 2440
Fragments arranged by size:
2440 1485 411 398 274 231 147 6
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2090 2120 2198 2228 1178
Size: 30 165 30 687 30 78 30 4342
Fragments arranged by size:
4342 687 165 78 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5392
BseYI C'CCAG_C
Cuts at: 2033 2262 4595 5091 2033
Size: 229 2333 496 2334
Fragments arranged by size:
2334 2333 496 229
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4819
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2842 3603 4631 1540
Size: 275 186 841 761 1028 2301
Fragments arranged by size:
2301 1028 841 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2679 3453 3538 4609 2679
Size: 774 85 1071 3462
Fragments arranged by size:
3462 1071 774 85
BsmI GAATG_Cn'
Cuts at: 2903 2996 2903
Size: 93 5299
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2034 2153 2679 2711 3453 3538 4609
Size: 399 244 119 526 32 742 85 1071
Cuts at: 4609 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 526 399 244 119 85
32
BspEI T'CCGG_A
Cuts at: 5055 5055
Size: 5392
BspHI T'CATG_A
Cuts at: 2490 3281 5011 2490
Size: 791 1730 2871
Fragments arranged by size:
2871 1730 791
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2747 844
Size: 1903 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3279 1394
Size: 429 1456 3507
Fragments arranged by size:
3507 1456 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2227 3887 4061 65
Size: 1183 979 1660 174 1396
Fragments arranged by size:
1660 1396 1183 979 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2103 4036 4223 1700
Size: 110 293 1933 187 2869
Fragments arranged by size:
2869 1933 293 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5392
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5392
BssSI C'ACGA_G
Cuts at: 3452 4464 3452
Size: 1012 4380
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5392
BstBI TT'CG_AA
Cuts at: 4196 4196
Size: 5392
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5392
BstYI r'GATC_y
Cuts at: 1972 2139 2722 3089 3474 3491 4943 5029 5041
Size: 167 583 367 385 17 1452 86 12
Cuts at: 5041 5387 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 583 385 367 346 167 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3901
Fragments arranged by size:
3901 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4653
Fragments arranged by size:
4653 683 56
BtsI GCAGTG_nn'
Cuts at: 2194 2908 3702 3722 2194
Size: 714 794 20 3864
Fragments arranged by size:
3864 794 714 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5392
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5357
DraI TTT'AAA
Cuts at: 2696 2696
Size: 5392
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4399 809
Size: 507 3083 1802
Fragments arranged by size:
3083 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2839 3728
Size: 54 1425 50 275 18 177 832 889
Cuts at: 3728 5357 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 832 275 177 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2839 1537
Size: 275 1027 4090
Fragments arranged by size:
4090 1027 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2289 2522 3322 1353
Size: 936 233 800 3423
Fragments arranged by size:
3423 936 800 233
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3979 4353 4499 387
Size: 1064 138 2390 374 146 1280
Fragments arranged by size:
2390 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2291 2477 2495 3455 4052 4839 5107
Size: 393 659 186 18 960 597 787 268
Cuts at: 5107 1239
Size: 1524
Fragments arranged by size:
1524 960 787 659 597 393 268 186
18
EcoO109I rG'GnC_Cy
Cuts at: 2596 2596
Size: 5392
EcoRI G'AATT_C
Cuts at: 2701 3079 2701
Size: 378 5014
FspI TGC'GCA
Cuts at: 3898 3898
Size: 5392
HaeII r_GCGC'y
Cuts at: 1678 2214 4539 1678
Size: 536 2325 2531
Fragments arranged by size:
2531 2325 536
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4033 4065 4107 4139 1373
Size: 32 135 32 2461 32 42 32 2626
Fragments arranged by size:
2626 2461 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2736 2982 669
Size: 988 281 798 246 3079
Fragments arranged by size:
3079 988 798 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5392
HpaI GTT'AAC
Cuts at: 2982 2982
Size: 5392
KpnI G_GTAC'C
Cuts at: 2110 2717 2110
Size: 607 4785
MfeI C'AATT_G
Cuts at: 2991 2991
Size: 5392
MluI A'CGCG_T
Cuts at: 5268 5268
Size: 5392
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4506 4690 1305
Size: 3201 184 2007
Fragments arranged by size:
3201 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 10
Size: 54 1425 343 177 3393
Fragments arranged by size:
3393 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2525 3350 3709 3868 518
Size: 648 1359 825 359 159 2042
Fragments arranged by size:
2042 1359 825 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2072 2269 2455 2808 3485 4633 4878 1606
Size: 466 197 186 353 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 466 353 245 197 186
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3901
Fragments arranged by size:
3901 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5392
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5392
NotI GC'GGCC_GC
Cuts at: 2839 2839
Size: 5392
NspI r_CATG'y
Cuts at: 1132 1229 2750 4295 1132
Size: 97 1521 1545 2229
Fragments arranged by size:
2229 1545 1521 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5392
PciI A'CATG_T
Cuts at: 4291 4291
Size: 5392
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2040 2044 1108
Size: 932 4 4456
Fragments arranged by size:
4456 932 4
PmeI GTTT'AAAC
Cuts at: 2696 2696
Size: 5392
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2198 2230 3461 3493 4974 5006 2198
Size: 32 1231 32 1481 32 2584
Fragments arranged by size:
2584 1481 1231 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5392
PsiI TTA'TAA
Cuts at: 2962 2962
Size: 5392
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2116 2148 2116
Size: 32 5360
PstI C_TGCA'G
Cuts at: 830 2744 830
Size: 1914 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5392
PvuII CAG'CTG
Cuts at: 2072 2269 2455 2072
Size: 197 186 5009
Fragments arranged by size:
5009 197 186
SalI G'TCGA_C
Cuts at: 1936 2734 1936
Size: 798 4594
SbfI CC_TGCA'GG
Cuts at: 2744 2744
Size: 5392
ScaI AGT'ACT
Cuts at: 3640 5352 3640
Size: 1712 3680
SfcI C'TryA_G
Cuts at: 826 1045 2667 2740 3875 4556 4747 826
Size: 219 1622 73 1135 681 191 1471
Fragments arranged by size:
1622 1471 1135 681 219 191 73
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5392
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5392
SmaI CCC'GGG
Cuts at: 1872 2719 1872
Size: 847 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3496 4397 4659 820
Size: 197 926 1553 901 262 1553
Fragments arranged by size:
1553 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3183 493
Size: 2690 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5392
SphI G_CATG'C
Cuts at: 1132 2750 1132
Size: 1618 3774
SspI AAT'ATT
Cuts at: 5 52 3316 5
Size: 47 3264 2081
Fragments arranged by size:
3264 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5392
StyI C'CwwG_G
Cuts at: 513 1065 2004 2332 2445 2831 513
Size: 552 939 328 113 386 3074
Fragments arranged by size:
3074 939 552 386 328 113
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2694 3419 3436 3589 3774 4219 325
Size: 1255 1114 725 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1114 725 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3638 5350 96
Size: 274 80 33 51 1443 1661 1712 138
Fragments arranged by size:
1712 1661 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2645 2677 5352 5384 2645
Size: 32 2675 32 2653
Fragments arranged by size:
2675 2653 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5392
XbaI T'CTAG_A
Cuts at: 2728 2728
Size: 5392
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2041 2011
Size: 30 5362
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5392
XmaI C'CCGG_G
Cuts at: 1870 2717 1870
Size: 847 4545
XmnI GAAnn'nnTTC
Cuts at: 3521 3521
Size: 5392
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3200 276
Size: 53 83 186 936 1666 2468
Fragments arranged by size:
2468 1666 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI