Restriction map for
pFN21AE6681
(Circular) (Six-base) MAPSORT of: pFN21AE6681.seq Check: 666 from: 1 to: 5725
pFN21AE6681 5725 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2803 2803
Size: 5725
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3535 278
Size: 53 83 186 936 1999 2468
Fragments arranged by size:
2468 1999 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3068 1937
Size: 1131 4594
Acc65I G'GTAC_C
Cuts at: 3046 3046
Size: 5725
AclI AA'CG_TT
Cuts at: 1563 3852 4225 1563
Size: 2289 373 3063
Fragments arranged by size:
3063 2289 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2448 2585 2907 2996 3788 5172 5440 1632
Size: 816 137 322 89 792 1384 268 1917
Fragments arranged by size:
1917 1384 816 792 322 268 137 89
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5528
AflIII A'CryG_T
Cuts at: 1203 4624 5601 1203
Size: 3421 977 1327
Fragments arranged by size:
3421 1327 977
AgeI A'CCGG_T
Cuts at: 4556 4556
Size: 5725
AhdI GACnn_n'nnGTC
Cuts at: 4454 4454
Size: 5725
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5725
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5725
AlwNI CAG_nnn'CTG
Cuts at: 3468 5040 3468
Size: 1572 4153
ApaI G_GGCC'C
Cuts at: 2306 2306
Size: 5725
ApaLI G'TGCA_C
Cuts at: 3782 4938 3782
Size: 1156 4569
ApoI r'AATT_y
Cuts at: 1493 2453 2497 3034 3260 3412 5521 1493
Size: 960 44 537 226 152 2109 1697
Fragments arranged by size:
2109 1697 960 537 226 152 44
AseI AT'TA_AT
Cuts at: 160 1032 4279 160
Size: 872 3247 1606
Fragments arranged by size:
3247 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5725
AvaI C'yCGr_G
Cuts at: 1870 1943 2382 3050 1870
Size: 73 439 668 4545
Fragments arranged by size:
4545 668 439 73
BamHI G'GATC_C
Cuts at: 3055 3422 3055
Size: 367 5358
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3046 4501 618
Size: 325 217 627 1259 1455 1842
Fragments arranged by size:
1842 1455 1259 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2306 3044 1391
Size: 915 738 4072
Fragments arranged by size:
4072 915 738
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2295 2364 2625 928
Size: 971 396 69 261 4028
Fragments arranged by size:
4028 971 396 261 69
BbvCI CC'TCA_GC
Cuts at: 2060 2060
Size: 5725
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3916 3950 1903
Size: 34 1979 34 3678
Fragments arranged by size:
3678 1979 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3617 4827 3617
Size: 1210 4515
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5725
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4336 136
Size: 107 122 71 631 605 2664 1525
Fragments arranged by size:
2664 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2728 5720 2728
Size: 2992 2733
BlpI GC'TnA_GC
Cuts at: 3142 3142
Size: 5725
Bme1580I G_kGCm'C
Cuts at: 1790 2306 3786 4942 1790
Size: 516 1480 1156 2573
Fragments arranged by size:
2573 1480 1156 516
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5725
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3456 4414 442
Size: 416 53 83 399 24 2039 958 1753
Fragments arranged by size:
2039 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5725
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4385 1239
Size: 3146 2579
Bpu10I CC'TnA_GC
Cuts at: 2060 2060
Size: 5725
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2562 3850 4715 5013 2562
Size: 1288 865 298 3274
Fragments arranged by size:
3274 1288 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2360 2591 2720 4388 882
Size: 617 861 231 129 1668 2219
Fragments arranged by size:
2219 1668 861 617 231 129
BsaAI yAC'GTr
Cuts at: 493 1204 3516 493
Size: 711 2312 2702
Fragments arranged by size:
2702 2312 711
BsaBI GATnn'nnATC
Cuts at: 5719 5719
Size: 5725
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3532 3914 4549 275
Size: 53 83 186 936 1999 382 635 1451
Fragments arranged by size:
1999 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4158 4556 4830 4977 5388 4158
Size: 398 274 147 411 4495
Fragments arranged by size:
4495 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5500
Fragments arranged by size:
5500 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2628 1186
Size: 1442 4283
BseYI C'CCAG_C
Cuts at: 2759 4928 5424 2759
Size: 2169 496 3060
Fragments arranged by size:
3060 2169 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2232 1163
Size: 573 496 4656
Fragments arranged by size:
4656 573 496
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3175 3936 4964 1540
Size: 275 186 1174 761 1028 2301
Fragments arranged by size:
2301 1174 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2220 3786 3871 4942 2220
Size: 1566 85 1071 3003
Fragments arranged by size:
3003 1566 1071 85
BsmI GAATG_Cn'
Cuts at: 3236 3329 3236
Size: 93 5632
BsmBI CGTCTCn'nnnn_
Cuts at: 2079 2079
Size: 5725
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2220 2306 3044 3786 3871 4942 1391
Size: 399 430 86 738 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 738 430 399 86 85
BspEI T'CCGG_A
Cuts at: 5388 5388
Size: 5725
BspHI T'CATG_A
Cuts at: 3614 5344 3614
Size: 1730 3995
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2803 3080 844
Size: 1959 277 3489
Fragments arranged by size:
3489 1959 277
BsrBI CCG'CTC
Cuts at: 1394 1823 3612 1394
Size: 429 1789 3507
Fragments arranged by size:
3507 1789 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2529 4220 4394 65
Size: 1183 1281 1691 174 1396
Fragments arranged by size:
1691 1396 1281 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4369 4556 1700
Size: 110 2559 187 2869
Fragments arranged by size:
2869 2559 187 110
BsrGI T'GTAC_A
Cuts at: 96 2736 96
Size: 2640 3085
BssHII G'CGCG_C
Cuts at: 1925 2033 1925
Size: 108 5617
BssSI C'ACGA_G
Cuts at: 2778 3785 4797 2778
Size: 1007 1012 3706
Fragments arranged by size:
3706 1012 1007
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5725
BstBI TT'CG_AA
Cuts at: 4529 4529
Size: 5725
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5725
BstXI CCAn_nnnn'nTGG
Cuts at: 2347 2347
Size: 5725
BstYI r'GATC_y
Cuts at: 1972 2728 3055 3422 3807 3824 5276 5362 5374
Size: 756 327 367 385 17 1452 86 12
Cuts at: 5374 5720 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 756 385 367 346 327 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2793 2838 513
Size: 552 939 789 45 3400
Fragments arranged by size:
3400 939 789 552 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4986
Fragments arranged by size:
4986 683 56
BtsI GCAGTG_nn'
Cuts at: 2151 2220 2553 3241 4035 4055 2151
Size: 69 333 688 794 20 3821
Fragments arranged by size:
3821 794 688 333 69 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5725
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5690
DraI TTT'AAA
Cuts at: 2908 3029 2908
Size: 121 5604
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4732 809
Size: 507 3416 1802
Fragments arranged by size:
3416 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3172 4061 5690
Size: 54 1425 50 275 18 1342 889 1629
Cuts at: 5690 8
Size: 43
Fragments arranged by size:
1629 1425 1342 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3172 1537
Size: 275 1360 4090
Fragments arranged by size:
4090 1360 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3655 1353
Size: 2302 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2366 4312 4686 4832 387
Size: 1064 138 777 1946 374 146 1280
Fragments arranged by size:
1946 1280 1064 777 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2448 2585 2907 2996 3788 4385 5172
Size: 393 816 137 322 89 792 597 787
Cuts at: 5172 5440 1239
Size: 268 1524
Fragments arranged by size:
1524 816 792 787 597 393 322 268
137 89
EcoNI CCTnn'n_nnAGG
Cuts at: 2580 2580
Size: 5725
EcoO109I rG'GnC_Cy
Cuts at: 2303 2386 2934 2303
Size: 83 548 5094
Fragments arranged by size:
5094 548 83
EcoRI G'AATT_C
Cuts at: 3034 3412 3034
Size: 378 5347
EcoRV GAT'ATC
Cuts at: 2089 2089
Size: 5725
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2953 2953
Size: 5725
FspI TGC'GCA
Cuts at: 4231 4231
Size: 5725
HaeII r_GCGC'y
Cuts at: 1678 2012 2057 4872 1678
Size: 334 45 2815 2531
Fragments arranged by size:
2815 2531 334 45
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4366 4398 4440 4472 1373
Size: 32 135 32 2794 32 42 32 2626
Fragments arranged by size:
2794 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2329 3069 3315 669
Size: 988 281 391 740 246 3079
Fragments arranged by size:
3079 988 740 391 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5725
HpaI GTT'AAC
Cuts at: 3315 3315
Size: 5725
KpnI G_GTAC'C
Cuts at: 3050 3050
Size: 5725
MfeI C'AATT_G
Cuts at: 3324 3324
Size: 5725
MluI A'CGCG_T
Cuts at: 5601 5601
Size: 5725
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4839 5023 1305
Size: 3534 184 2007
Fragments arranged by size:
3534 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3903
Fragments arranged by size:
3903 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3683 4042 4201 518
Size: 648 2517 359 159 2042
Fragments arranged by size:
2517 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2084 3141 3818 4966 5211 1606
Size: 478 1057 677 1148 245 2120
Fragments arranged by size:
2120 1148 1057 677 478 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2793 513
Size: 552 939 789 3445
Fragments arranged by size:
3445 939 789 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5725
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5725
NotI GC'GGCC_GC
Cuts at: 3172 3172
Size: 5725
NspI r_CATG'y
Cuts at: 1132 1229 2968 3083 4628 1132
Size: 97 1739 115 1545 2229
Fragments arranged by size:
2229 1739 1545 115 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5725
PciI A'CATG_T
Cuts at: 4624 4624
Size: 5725
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5725
PfoI T'CCnGG_A
Cuts at: 2844 2844
Size: 5725
PmeI GTTT'AAAC
Cuts at: 3029 3029
Size: 5725
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3794 3826 5307 5339 3794
Size: 32 1481 32 4180
Fragments arranged by size:
4180 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2386 2386
Size: 5725
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5725
PsiI TTA'TAA
Cuts at: 3295 3295
Size: 5725
PspOMI G'GGCC_C
Cuts at: 2302 2302
Size: 5725
PstI C_TGCA'G
Cuts at: 830 3077 830
Size: 2247 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5725
PvuII CAG'CTG
Cuts at: 2084 2084
Size: 5725
SalI G'TCGA_C
Cuts at: 1936 3067 1936
Size: 1131 4594
SbfI CC_TGCA'GG
Cuts at: 3077 3077
Size: 5725
ScaI AGT'ACT
Cuts at: 3973 5685 3973
Size: 1712 4013
SfcI C'TryA_G
Cuts at: 826 1045 3073 4208 4889 5080 826
Size: 219 2028 1135 681 191 1471
Fragments arranged by size:
2028 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5725
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5725
SmaI CCC'GGG
Cuts at: 1872 2384 3052 1872
Size: 512 668 4545
Fragments arranged by size:
4545 668 512
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2577 3829 4730 4992 820
Size: 197 926 634 1252 901 262 1553
Fragments arranged by size:
1553 1252 926 901 634 262 197
SnaBI TAC'GTA
Cuts at: 493 3516 493
Size: 3023 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5725
SphI G_CATG'C
Cuts at: 1132 3083 1132
Size: 1951 3774
SspI AAT'ATT
Cuts at: 5 52 3649 5
Size: 47 3597 2081
Fragments arranged by size:
3597 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5725
StyI C'CwwG_G
Cuts at: 513 1065 2004 2793 2947 3164 513
Size: 552 939 789 154 217 3074
Fragments arranged by size:
3074 939 789 552 217 154
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3752 3769 3922 4107 4552 325
Size: 1255 2172 17 153 185 445 1498
Fragments arranged by size:
2172 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2127 2736 3971
Size: 274 80 33 51 1443 150 609 1235
Cuts at: 3971 5683 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1235 609 274 150 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5685 5717 5685
Size: 32 5693
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5725
XbaI T'CTAG_A
Cuts at: 2593 3061 2593
Size: 468 5257
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5725
XmaI C'CCGG_G
Cuts at: 1870 2382 3050 1870
Size: 512 668 4545
Fragments arranged by size:
4545 668 512
XmnI GAAnn'nnTTC
Cuts at: 2622 3854 2622
Size: 1232 4493
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3533 276
Size: 53 83 186 936 1999 2468
Fragments arranged by size:
2468 1999 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BstZ17I Bsu36I DraIII EcoICRI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI