Restriction map for
pFN21AE6679
(Circular) (Six-base) MAPSORT of: pFN21AE6679.seq Check: 6268 from: 1 to: 5689
pFN21AE6679 5689 bp
With 182 enzymes: SgfI *
October 25, 2019 12:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2245 2915 2245
Size: 670 5019
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3499 278
Size: 53 83 186 936 1963 2468
Fragments arranged by size:
2468 1963 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2283 3032 1937
Size: 346 749 4594
Fragments arranged by size:
4594 749 346
Acc65I G'GTAC_C
Cuts at: 3010 3010
Size: 5689
AclI AA'CG_TT
Cuts at: 1563 3816 4189 1563
Size: 2253 373 3063
Fragments arranged by size:
3063 2253 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2272 2349 3752 5136 5404 1632
Size: 640 77 1403 1384 268 1917
Fragments arranged by size:
1917 1403 1384 640 268 77
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5492
AflIII A'CryG_T
Cuts at: 1203 4588 5565 1203
Size: 3385 977 1327
Fragments arranged by size:
3385 1327 977
AgeI A'CCGG_T
Cuts at: 4520 4520
Size: 5689
AhdI GACnn_n'nnGTC
Cuts at: 4418 4418
Size: 5689
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5689
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5689
AlwNI CAG_nnn'CTG
Cuts at: 3432 5004 3432
Size: 1572 4117
ApaI G_GGCC'C
Cuts at: 2849 2903 2912 2849
Size: 54 9 5626
Fragments arranged by size:
5626 54 9
ApaLI G'TGCA_C
Cuts at: 3746 4902 3746
Size: 1156 4533
ApoI r'AATT_y
Cuts at: 1493 2022 2305 2515 2998 3224 3376 5485 1493
Size: 529 283 210 483 226 152 2109 1697
Fragments arranged by size:
2109 1697 529 483 283 226 210 152
AseI AT'TA_AT
Cuts at: 160 1032 2442 4243 160
Size: 872 1410 1801 1606
Fragments arranged by size:
1801 1606 1410 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5689
AvaI C'yCGr_G
Cuts at: 1870 1943 2815 2834 2905 2914 3014 1870
Size: 73 872 19 71 9 100 4545
Fragments arranged by size:
4545 872 100 73 71 19 9
BamHI G'GATC_C
Cuts at: 3019 3386 3019
Size: 367 5322
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3010 4465 618
Size: 325 217 627 1223 1455 1842
Fragments arranged by size:
1842 1455 1223 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2849 2903 2912 2950 3008 1391
Size: 1458 54 9 38 58 4072
Fragments arranged by size:
4072 1458 58 54 38 9
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2015 928
Size: 971 116 4602
Fragments arranged by size:
4602 971 116
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2493 2527 3880 3914 1903
Size: 34 556 34 1353 34 3678
Fragments arranged by size:
3678 1353 556 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3581 4791 3581
Size: 1210 4479
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5689
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2907 4300 136
Size: 107 122 71 631 605 1235 1393 1525
Fragments arranged by size:
1525 1393 1235 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5684 5684
Size: 5689
BlpI GC'TnA_GC
Cuts at: 3106 3106
Size: 5689
Bme1580I G_kGCm'C
Cuts at: 1790 2849 2903 2912 3750 4906 1790
Size: 1059 54 9 838 1156 2573
Fragments arranged by size:
2573 1156 1059 838 54 9
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5689
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2310 2533 3420
Size: 416 53 83 399 24 893 223 887
Cuts at: 3420 4378 442
Size: 958 1753
Fragments arranged by size:
1753 958 893 887 416 399 223 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5689
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2874 2934 4349 1239
Size: 1635 60 1415 2579
Fragments arranged by size:
2579 1635 1415 60
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2670 3814 4679 4977 2670
Size: 1144 865 298 3382
Fragments arranged by size:
3382 1144 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4352 882
Size: 617 2853 2219
Fragments arranged by size:
2853 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3480 493
Size: 711 2276 2702
Fragments arranged by size:
2702 2276 711
BsaBI GATnn'nnATC
Cuts at: 2720 5683 2720
Size: 2963 2726
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3496 3878 4513 275
Size: 53 83 186 936 1963 382 635 1451
Fragments arranged by size:
1963 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4122 4520 4794 4941 5352 4122
Size: 398 274 147 411 4459
Fragments arranged by size:
4459 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5464
Fragments arranged by size:
5464 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2584 1186
Size: 1398 4291
BseYI C'CCAG_C
Cuts at: 2662 2786 4892 5388 2662
Size: 124 2106 496 2963
Fragments arranged by size:
2963 2106 496 124
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2065 1163
Size: 573 329 4787
Fragments arranged by size:
4787 573 329
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3139 3900 4928 1540
Size: 275 186 1138 761 1028 2301
Fragments arranged by size:
2301 1138 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2950 3750 3835 4906 2950
Size: 800 85 1071 3733
Fragments arranged by size:
3733 1071 800 85
BsmI GAATG_Cn'
Cuts at: 3200 3293 3200
Size: 93 5596
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2849 2903 2912 2950 3008 3750 3835
Size: 399 1059 54 9 38 58 742 85
Cuts at: 3835 4906 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 1059 742 399 85 58 54
38 9
BspEI T'CCGG_A
Cuts at: 5352 5352
Size: 5689
BspHI T'CATG_A
Cuts at: 2553 3578 5308 2553
Size: 1025 1730 2934
Fragments arranged by size:
2934 1730 1025
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2021 2245 2915 3044 844
Size: 1177 224 670 129 3489
Fragments arranged by size:
3489 1177 670 224 129
BsrBI CCG'CTC
Cuts at: 1394 1823 3576 1394
Size: 429 1753 3507
Fragments arranged by size:
3507 1753 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2249 4184 4358 65
Size: 1183 1001 1935 174 1396
Fragments arranged by size:
1935 1396 1183 1001 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4333 4520 1700
Size: 110 2523 187 2869
Fragments arranged by size:
2869 2523 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5689
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5689
BssSI C'ACGA_G
Cuts at: 3749 4761 3749
Size: 1012 4677
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5689
BstBI TT'CG_AA
Cuts at: 2356 2513 4493 2356
Size: 157 1980 3552
Fragments arranged by size:
3552 1980 157
BstEII G'GTnAC_C
Cuts at: 1184 2882 1184
Size: 1698 3991
BstYI r'GATC_y
Cuts at: 1972 3019 3386 3771 3788 5240 5326 5338 5684
Size: 1047 367 385 17 1452 86 12 346
Cuts at: 5684 1972
Size: 1977
Fragments arranged by size:
1977 1452 1047 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2895 2895
Size: 5689
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5137
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4950
Fragments arranged by size:
4950 683 56
BtsI GCAGTG_nn'
Cuts at: 3205 3999 4019 3205
Size: 794 20 4875
Fragments arranged by size:
4875 794 20
ClaI AT'CG_AT
Cuts at: 1554 2868 1554
Size: 1314 4375
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5654
DraI TTT'AAA
Cuts at: 2993 2993
Size: 5689
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4696 809
Size: 507 3380 1802
Fragments arranged by size:
3380 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2152 2446 3136
Size: 54 1425 50 275 18 322 294 690
Cuts at: 3136 4025 5654 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 690 322 294 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3136 1537
Size: 275 1324 4090
Fragments arranged by size:
4090 1324 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2212 3619 1353
Size: 859 1407 3423
Fragments arranged by size:
3423 1407 859
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4276 4650 4796 387
Size: 1064 138 2687 374 146 1280
Fragments arranged by size:
2687 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2948 2948
Size: 5689
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2272 2349 2874 2934 3752 4349 5136
Size: 393 640 77 525 60 818 597 787
Cuts at: 5136 5404 1239
Size: 268 1524
Fragments arranged by size:
1524 818 787 640 597 525 393 268
77 60
EcoO109I rG'GnC_Cy
Cuts at: 2810 2828 2846 2891 2899 2900 2909 2918 2810
Size: 18 18 45 8 1 9 9 5581
Fragments arranged by size:
5581 45 18 18 9 9 8 1
EcoRI G'AATT_C
Cuts at: 2998 3376 2998
Size: 378 5311
FspI TGC'GCA
Cuts at: 4195 4195
Size: 5689
HaeII r_GCGC'y
Cuts at: 1678 2976 4836 1678
Size: 1298 1860 2531
Fragments arranged by size:
2531 1860 1298
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2085 2117 2168 2200 4330
Size: 32 135 32 513 32 51 32 2130
Cuts at: 4330 4362 4404 4436 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2130 513 135 51 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2593 3033 3279 669
Size: 988 281 655 440 246 3079
Fragments arranged by size:
3079 988 655 440 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5689
HpaI GTT'AAC
Cuts at: 3279 3279
Size: 5689
KpnI G_GTAC'C
Cuts at: 3014 3014
Size: 5689
MfeI C'AATT_G
Cuts at: 2637 3288 2637
Size: 651 5038
MluI A'CGCG_T
Cuts at: 5565 5565
Size: 5689
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2321 4803 4987 1305
Size: 1016 2482 184 2007
Fragments arranged by size:
2482 2007 1016 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2154 2448 10
Size: 54 1425 343 322 294 3251
Fragments arranged by size:
3251 1425 343 322 294 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3647 4006 4165 518
Size: 648 2481 359 159 2042
Fragments arranged by size:
2481 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2075 3105 3782 4930 5175 1606
Size: 469 1030 677 1148 245 2120
Fragments arranged by size:
2120 1148 1030 677 469 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5137
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5689
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5689
NotI GC'GGCC_GC
Cuts at: 3136 3136
Size: 5689
NspI r_CATG'y
Cuts at: 1132 1229 3047 4592 1132
Size: 97 1818 1545 2229
Fragments arranged by size:
2229 1818 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2061 1348
Size: 713 4976
PciI A'CATG_T
Cuts at: 4588 4588
Size: 5689
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5689
PfoI T'CCnGG_A
Cuts at: 2052 2052
Size: 5689
PmeI GTTT'AAAC
Cuts at: 2993 2993
Size: 5689
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3758 3790 5271 5303 3758
Size: 32 1481 32 4144
Fragments arranged by size:
4144 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2810 2828 2891 2918 2810
Size: 18 63 27 5581
Fragments arranged by size:
5581 63 27 18
PshAI GACnn'nnGTC
Cuts at: 1613 2889 1613
Size: 1276 4413
PsiI TTA'TAA
Cuts at: 3259 3259
Size: 5689
PspOMI G'GGCC_C
Cuts at: 2845 2899 2908 2845
Size: 54 9 5626
Fragments arranged by size:
5626 54 9
PstI C_TGCA'G
Cuts at: 830 2074 2926 3041 830
Size: 1244 852 115 3478
Fragments arranged by size:
3478 1244 852 115
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5689
PvuII CAG'CTG
Cuts at: 2075 2075
Size: 5689
SacI G_AGCT'C
Cuts at: 2950 2950
Size: 5689
SalI G'TCGA_C
Cuts at: 1936 3031 1936
Size: 1095 4594
SapI GCTCTTCn'nnn_
Cuts at: 2212 2212
Size: 5689
SbfI CC_TGCA'GG
Cuts at: 2926 3041 2926
Size: 115 5574
ScaI AGT'ACT
Cuts at: 3937 5649 3937
Size: 1712 3977
SfcI C'TryA_G
Cuts at: 826 1045 2070 2922 3037 4172 4853 5044 826
Size: 219 1025 852 115 1135 681 191 1471
Fragments arranged by size:
1471 1135 1025 852 681 219 191 115
SfiI GGCCn_nnn'nGGCC
Cuts at: 2907 2907
Size: 5689
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5689
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5689
SmaI CCC'GGG
Cuts at: 1872 2817 2836 2907 2916 3016 1872
Size: 945 19 71 9 100 4545
Fragments arranged by size:
4545 945 100 71 19 9
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2685 3793 4694 4956 820
Size: 197 926 742 1108 901 262 1553
Fragments arranged by size:
1553 1108 926 901 742 262 197
SnaBI TAC'GTA
Cuts at: 493 3480 493
Size: 2987 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5689
SphI G_CATG'C
Cuts at: 1132 3047 1132
Size: 1915 3774
SspI AAT'ATT
Cuts at: 5 52 3613 5
Size: 47 3561 2081
Fragments arranged by size:
3561 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5689
StyI C'CwwG_G
Cuts at: 513 1065 3128 513
Size: 552 2063 3074
Fragments arranged by size:
3074 2063 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2901 3716 3733 3886 4071 4516 325
Size: 1255 1321 815 17 153 185 445 1498
Fragments arranged by size:
1498 1321 1255 815 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3935 5647 96
Size: 274 80 33 51 1443 1958 1712 138
Fragments arranged by size:
1958 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5649 5681 5649
Size: 32 5657
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5689
XbaI T'CTAG_A
Cuts at: 2213 3025 2213
Size: 812 4877
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5689
XmaI C'CCGG_G
Cuts at: 1870 2815 2834 2905 2914 3014 1870
Size: 945 19 71 9 100 4545
Fragments arranged by size:
4545 945 100 71 19 9
XmnI GAAnn'nnTTC
Cuts at: 3818 3818
Size: 5689
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3497 276
Size: 53 83 186 936 1963 2468
Fragments arranged by size:
2468 1963 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspXI PsrI RsrII SacII
SanDI SexAI SfoI SrfI SwaI XcmI