Restriction map for
pFN21AE6677
(Circular) (Six-base) MAPSORT of: pFN21AE6677.seq Check: 9394 from: 1 to: 5596
pFN21AE6677 5596 bp
With 182 enzymes: SgfI *
October 17, 2018 20:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2374 2374
Size: 5596
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3406 278
Size: 53 83 186 936 1870 2468
Fragments arranged by size:
2468 1870 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2939 1937
Size: 1002 4594
Acc65I G'GTAC_C
Cuts at: 2172 2917 2172
Size: 745 4851
AclI AA'CG_TT
Cuts at: 1563 3723 4096 1563
Size: 2160 373 3063
Fragments arranged by size:
3063 2160 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2171 2249 3659 5043 5311 1632
Size: 539 78 1410 1384 268 1917
Fragments arranged by size:
1917 1410 1384 539 268 78
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5399
AflIII A'CryG_T
Cuts at: 1203 2094 4495 5472 1203
Size: 891 2401 977 1327
Fragments arranged by size:
2401 1327 977 891
AgeI A'CCGG_T
Cuts at: 4427 4427
Size: 5596
AhdI GACnn_n'nnGTC
Cuts at: 4325 4325
Size: 5596
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5596
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5596
AlwNI CAG_nnn'CTG
Cuts at: 3339 4911 3339
Size: 1572 4024
ApaLI G'TGCA_C
Cuts at: 3653 4809 3653
Size: 1156 4440
ApoI r'AATT_y
Cuts at: 1493 2708 2848 2905 3131 3283 5392 1493
Size: 1215 140 57 226 152 2109 1697
Fragments arranged by size:
2109 1697 1215 226 152 140 57
AseI AT'TA_AT
Cuts at: 160 1032 4150 160
Size: 872 3118 1606
Fragments arranged by size:
3118 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5596
AvaI C'yCGr_G
Cuts at: 1870 1943 2043 2504 2921 1870
Size: 73 100 461 417 4545
Fragments arranged by size:
4545 461 417 100 73
BamHI G'GATC_C
Cuts at: 2926 3293 2926
Size: 367 5229
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2172 2917 4372 618
Size: 325 217 627 385 745 1455 1842
Fragments arranged by size:
1842 1455 745 627 385 325 217
BanII G_rGCy'C
Cuts at: 1391 2339 2682 2915 1391
Size: 948 343 233 4072
Fragments arranged by size:
4072 948 343 233
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4625
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3787 3821 1903
Size: 34 1850 34 3678
Fragments arranged by size:
3678 1850 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3488 4698 3488
Size: 1210 4386
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5596
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4207 136
Size: 107 122 71 631 605 2535 1525
Fragments arranged by size:
2535 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5591 5591
Size: 5596
BlpI GC'TnA_GC
Cuts at: 3013 3013
Size: 5596
Bme1580I G_kGCm'C
Cuts at: 1790 3657 4813 1790
Size: 1867 1156 2573
Fragments arranged by size:
2573 1867 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5596
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3327 4285 442
Size: 416 53 83 399 24 1910 958 1753
Fragments arranged by size:
1910 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5596
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2501 4256 1239
Size: 1262 1755 2579
Fragments arranged by size:
2579 1755 1262
Bpu10I CC'TnA_GC
Cuts at: 2282 2282
Size: 5596
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2234 3721 4586 4884 2234
Size: 1487 865 298 2946
Fragments arranged by size:
2946 1487 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2564 4259 882
Size: 617 1065 1695 2219
Fragments arranged by size:
2219 1695 1065 617
BsaAI yAC'GTr
Cuts at: 493 1204 2218 3387 493
Size: 711 1014 1169 2702
Fragments arranged by size:
2702 1169 1014 711
BsaBI GATnn'nnATC
Cuts at: 5590 5590
Size: 5596
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2412 3403 3785 4420
Size: 53 83 186 936 879 991 382 635
Cuts at: 4420 275
Size: 1451
Fragments arranged by size:
1451 991 936 879 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4029 4427 4701 4848 5259 4029
Size: 398 274 147 411 4366
Fragments arranged by size:
4366 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5371
Fragments arranged by size:
5371 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2057 2138 2213 2270 2284 2522 1186
Size: 871 81 75 57 14 238 4260
Fragments arranged by size:
4260 871 238 81 75 57 14
BseYI C'CCAG_C
Cuts at: 4799 5295 4799
Size: 496 5100
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2089 2360 1163
Size: 573 353 271 4399
Fragments arranged by size:
4399 573 353 271
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3046 3807 4835 1540
Size: 275 186 1045 761 1028 2301
Fragments arranged by size:
2301 1045 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2067 2682 3657 3742 4813 2067
Size: 615 975 85 1071 2850
Fragments arranged by size:
2850 1071 975 615 85
BsmI GAATG_Cn'
Cuts at: 2410 3107 3200 2410
Size: 697 93 4806
Fragments arranged by size:
4806 697 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2067 2339 2682 2915 3657 3742 4813
Size: 399 277 272 343 233 742 85 1071
Cuts at: 4813 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 343 277 272 233
85
BspEI T'CCGG_A
Cuts at: 5259 5259
Size: 5596
BspHI T'CATG_A
Cuts at: 2748 3485 5215 2748
Size: 737 1730 3129
Fragments arranged by size:
3129 1730 737
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2374 2951 844
Size: 1530 577 3489
Fragments arranged by size:
3489 1530 577
BsrBI CCG'CTC
Cuts at: 1394 1823 3483 1394
Size: 429 1660 3507
Fragments arranged by size:
3507 1660 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4091 4265 65
Size: 1183 2843 174 1396
Fragments arranged by size:
2843 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4240 4427 1700
Size: 110 2430 187 2869
Fragments arranged by size:
2869 2430 187 110
BsrGI T'GTAC_A
Cuts at: 96 2091 96
Size: 1995 3601
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5596
BssSI C'ACGA_G
Cuts at: 3656 4668 3656
Size: 1012 4584
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2842 794
Size: 2048 3548
BstBI TT'CG_AA
Cuts at: 4400 4400
Size: 5596
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5596
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5596
BstYI r'GATC_y
Cuts at: 1972 2640 2926 3293 3678 3695 5147 5233 5245
Size: 668 286 367 385 17 1452 86 12
Cuts at: 5245 5591 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 668 385 367 346 286 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2157 513
Size: 552 1092 3952
Fragments arranged by size:
3952 1092 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2322 487
Size: 683 56 1096 3761
Fragments arranged by size:
3761 1096 683 56
BtsI GCAGTG_nn'
Cuts at: 2135 2445 3112 3906 3926 2135
Size: 310 667 794 20 3805
Fragments arranged by size:
3805 794 667 310 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5596
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2802 2837 529
Size: 35 2238 35 3288
Fragments arranged by size:
3288 2238 35 35
DraI TTT'AAA
Cuts at: 2900 2900
Size: 5596
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4603 809
Size: 507 3287 1802
Fragments arranged by size:
3287 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2421 2778 3043
Size: 54 1425 50 275 18 591 357 265
Cuts at: 3043 3932 5561 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 591 357 275 265 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3043 1537
Size: 275 1231 4090
Fragments arranged by size:
4090 1231 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3526 1353
Size: 2173 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4183 4557 4703 387
Size: 1064 138 2594 374 146 1280
Fragments arranged by size:
2594 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2680 2680
Size: 5596
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2171 2249 2501 3659 4256 5043 5311
Size: 393 539 78 252 1158 597 787 268
Cuts at: 5311 1239
Size: 1524
Fragments arranged by size:
1524 1158 787 597 539 393 268 252
78
EcoRI G'AATT_C
Cuts at: 2708 2905 3283 2708
Size: 197 378 5021
Fragments arranged by size:
5021 378 197
FspI TGC'GCA
Cuts at: 4102 4102
Size: 5596
HaeII r_GCGC'y
Cuts at: 1678 4743 1678
Size: 3065 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4237 4269 4311 4343 1373
Size: 32 135 32 2665 32 42 32 2626
Fragments arranged by size:
2665 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2940 3186 669
Size: 988 281 1002 246 3079
Fragments arranged by size:
3079 1002 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5596
HpaI GTT'AAC
Cuts at: 3186 3186
Size: 5596
KpnI G_GTAC'C
Cuts at: 2176 2921 2176
Size: 745 4851
MfeI C'AATT_G
Cuts at: 3195 3195
Size: 5596
MluI A'CGCG_T
Cuts at: 5472 5472
Size: 5596
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4710 4894 1305
Size: 3405 184 2007
Fragments arranged by size:
3405 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2423 2780 10
Size: 54 1425 343 591 357 2826
Fragments arranged by size:
2826 1425 591 357 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2703 2753 3554 3913 4072 518
Size: 648 843 694 50 801 359 159 2042
Fragments arranged by size:
2042 843 801 694 648 359 159 50
MspA1I CmG'CkG
Cuts at: 1606 2131 2479 3012 3689 4837 5082 1606
Size: 525 348 533 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 533 525 348 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5044
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5596
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5596
NotI GC'GGCC_GC
Cuts at: 3043 3043
Size: 5596
NspI r_CATG'y
Cuts at: 1132 1229 2098 2954 4499 1132
Size: 97 869 856 1545 2229
Fragments arranged by size:
2229 1545 869 856 97
PasI CC'CwG_GG
Cuts at: 1348 2142 1348
Size: 794 4802
PciI A'CATG_T
Cuts at: 2094 4495 2094
Size: 2401 3195
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5596
PmeI GTTT'AAAC
Cuts at: 2900 2900
Size: 5596
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3665 3697 5178 5210 3665
Size: 32 1481 32 4051
Fragments arranged by size:
4051 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5596
PsiI TTA'TAA
Cuts at: 3166 3166
Size: 5596
PstI C_TGCA'G
Cuts at: 830 2265 2590 2948 830
Size: 1435 325 358 3478
Fragments arranged by size:
3478 1435 358 325
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5596
PvuII CAG'CTG
Cuts at: 2131 2479 2131
Size: 348 5248
SacI G_AGCT'C
Cuts at: 2682 2682
Size: 5596
SalI G'TCGA_C
Cuts at: 1936 2938 1936
Size: 1002 4594
SbfI CC_TGCA'GG
Cuts at: 2948 2948
Size: 5596
ScaI AGT'ACT
Cuts at: 3844 5556 3844
Size: 1712 3884
SfcI C'TryA_G
Cuts at: 826 1045 2261 2462 2586 2944 4079 4760 4951
Size: 219 1216 201 124 358 1135 681 191
Cuts at: 4951 826
Size: 1471
Fragments arranged by size:
1471 1216 1135 681 358 219 201 191
124
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5596
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5596
SmaI CCC'GGG
Cuts at: 1872 2045 2923 1872
Size: 173 878 4545
Fragments arranged by size:
4545 878 173
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2249 3700 4601 4863 820
Size: 197 926 306 1451 901 262 1553
Fragments arranged by size:
1553 1451 926 901 306 262 197
SnaBI TAC'GTA
Cuts at: 493 3387 493
Size: 2894 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5596
SphI G_CATG'C
Cuts at: 1132 2954 1132
Size: 1822 3774
SspI AAT'ATT
Cuts at: 5 52 3520 5
Size: 47 3468 2081
Fragments arranged by size:
3468 2081 47
StuI AGG'CCT
Cuts at: 1517 2857 1517
Size: 1340 4256
StyI C'CwwG_G
Cuts at: 513 1065 2565 3035 513
Size: 552 1500 470 3074
Fragments arranged by size:
3074 1500 552 470
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3623 3640 3793 3978 4423 325
Size: 1255 2043 17 153 185 445 1498
Fragments arranged by size:
2043 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2091 3842 5554
Size: 274 80 33 51 1443 114 1751 1712
Cuts at: 5554 96
Size: 138
Fragments arranged by size:
1751 1712 1443 274 138 114 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5556 5588 5556
Size: 32 5564
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5596
XbaI T'CTAG_A
Cuts at: 2932 2932
Size: 5596
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2483 2752 2483
Size: 269 5327
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5596
XmaI C'CCGG_G
Cuts at: 1870 2043 2921 1870
Size: 173 878 4545
Fragments arranged by size:
4545 878 173
XmnI GAAnn'nnTTC
Cuts at: 3725 3725
Size: 5596
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3404 276
Size: 53 83 186 936 1870 2468
Fragments arranged by size:
2468 1870 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI