Restriction map for
pFN21AE6666
(Circular) (Six-base) MAPSORT of: pFN21AE6666.seq Check: 9715 from: 1 to: 5575
pFN21AE6666 5575 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2326 2326
Size: 5575
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2639 3385 278
Size: 53 83 186 936 1103 746 2468
Fragments arranged by size:
2468 1103 936 746 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2918 1937
Size: 981 4594
Acc65I G'GTAC_C
Cuts at: 2481 2896 2481
Size: 415 5160
AclI AA'CG_TT
Cuts at: 1563 3702 4075 1563
Size: 2139 373 3063
Fragments arranged by size:
3063 2139 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3638 5022 5290 1632
Size: 2006 1384 268 1917
Fragments arranged by size:
2006 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5378
AflIII A'CryG_T
Cuts at: 1203 4474 5451 1203
Size: 3271 977 1327
Fragments arranged by size:
3271 1327 977
AgeI A'CCGG_T
Cuts at: 4406 4406
Size: 5575
AhdI GACnn_n'nnGTC
Cuts at: 4304 4304
Size: 5575
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5575
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5575
AlwNI CAG_nnn'CTG
Cuts at: 3318 4890 3318
Size: 1572 4003
ApaLI G'TGCA_C
Cuts at: 3632 4788 3632
Size: 1156 4419
ApoI r'AATT_y
Cuts at: 1493 2525 2732 2884 3110 3262 5371 1493
Size: 1032 207 152 226 152 2109 1697
Fragments arranged by size:
2109 1697 1032 226 207 152 152
AseI AT'TA_AT
Cuts at: 160 1032 2414 4129 160
Size: 872 1382 1715 1606
Fragments arranged by size:
1715 1606 1382 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5575
AvaI C'yCGr_G
Cuts at: 1870 1943 2900 1870
Size: 73 957 4545
Fragments arranged by size:
4545 957 73
BamHI G'GATC_C
Cuts at: 2861 2905 3272 2861
Size: 44 367 5164
Fragments arranged by size:
5164 367 44
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2440 2481 2896 4351 618
Size: 325 217 627 653 41 415 1455 1842
Fragments arranged by size:
1842 1455 653 627 415 325 217 41
BanII G_rGCy'C
Cuts at: 1391 2239 2894 1391
Size: 848 655 4072
Fragments arranged by size:
4072 848 655
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4604
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3766 3800 1903
Size: 34 1829 34 3678
Fragments arranged by size:
3678 1829 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3467 4677 3467
Size: 1210 4365
BclI T'GATC_A
Cuts at: 1548 2173 1548
Size: 625 4950
BfrBI ATG'CAT
Cuts at: 2131 2131
Size: 5575
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4186 136
Size: 107 122 71 631 605 2514 1525
Fragments arranged by size:
2514 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2755 5570 2755
Size: 2815 2760
BlpI GC'TnA_GC
Cuts at: 2992 2992
Size: 5575
Bme1580I G_kGCm'C
Cuts at: 1790 2847 3636 4792 1790
Size: 1057 789 1156 2573
Fragments arranged by size:
2573 1156 1057 789
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5575
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2242 3306 4264
Size: 416 53 83 399 24 825 1064 958
Cuts at: 4264 442
Size: 1753
Fragments arranged by size:
1753 1064 958 825 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5575
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2195 2277 4235 1239
Size: 956 82 1958 2579
Fragments arranged by size:
2579 1958 956 82
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2108 3700 4565 4863 2108
Size: 1592 865 298 2820
Fragments arranged by size:
2820 1592 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4238 882
Size: 617 2739 2219
Fragments arranged by size:
2739 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3366 493
Size: 711 2162 2702
Fragments arranged by size:
2702 2162 711
BsaBI GATnn'nnATC
Cuts at: 5569 5569
Size: 5575
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2636 3382 3764 4399
Size: 53 83 186 936 1103 746 382 635
Cuts at: 4399 275
Size: 1451
Fragments arranged by size:
1451 1103 936 746 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4008 4406 4680 4827 5238 4008
Size: 398 274 147 411 4345
Fragments arranged by size:
4345 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5350
Fragments arranged by size:
5350 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2377 1186
Size: 1191 4384
BseYI C'CCAG_C
Cuts at: 2372 4778 5274 2372
Size: 2406 496 2673
Fragments arranged by size:
2673 2406 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5002
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3025 3786 4814 1540
Size: 275 186 1024 761 1028 2301
Fragments arranged by size:
2301 1028 1024 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2094 3636 3721 4792 2094
Size: 1542 85 1071 2877
Fragments arranged by size:
2877 1542 1071 85
BsmI GAATG_Cn'
Cuts at: 3086 3179 3086
Size: 93 5482
BsmBI CGTCTCn'nnnn_
Cuts at: 2643 2643
Size: 5575
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2094 2239 2847 2894 3636 3721 4792
Size: 399 304 145 608 47 742 85 1071
Cuts at: 4792 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 608 399 304 145 85
47
BspEI T'CCGG_A
Cuts at: 5238 5238
Size: 5575
BspHI T'CATG_A
Cuts at: 3464 5194 3464
Size: 1730 3845
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2326 2637 2930 844
Size: 1482 311 293 3489
Fragments arranged by size:
3489 1482 311 293
BsrBI CCG'CTC
Cuts at: 1394 1823 3462 1394
Size: 429 1639 3507
Fragments arranged by size:
3507 1639 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4070 4244 65
Size: 1183 2822 174 1396
Fragments arranged by size:
2822 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4219 4406 1700
Size: 110 2409 187 2869
Fragments arranged by size:
2869 2409 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5575
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5575
BssSI C'ACGA_G
Cuts at: 2840 3635 4647 2840
Size: 795 1012 3768
Fragments arranged by size:
3768 1012 795
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2454 794
Size: 1660 3915
BstBI TT'CG_AA
Cuts at: 4379 4379
Size: 5575
BstEII G'GTnAC_C
Cuts at: 1184 2338 1184
Size: 1154 4421
BstXI CCAn_nnnn'nTGG
Cuts at: 2152 2152
Size: 5575
BstYI r'GATC_y
Cuts at: 1972 2097 2755 2861 2905 3272 3657 3674 5126
Size: 125 658 106 44 367 385 17 1452
Cuts at: 5126 5212 5224 5570 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 658 385 367 346 125 106
86 44 17 12
Bsu36I CC'TnA_GG
Cuts at: 2436 2436
Size: 5575
BtgI C'CryG_G
Cuts at: 513 1065 2004 2145 513
Size: 552 939 141 3943
Fragments arranged by size:
3943 939 552 141
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4836
Fragments arranged by size:
4836 683 56
BtsI GCAGTG_nn'
Cuts at: 3091 3885 3905 3091
Size: 794 20 4761
Fragments arranged by size:
4761 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5575
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5540
DraI TTT'AAA
Cuts at: 2770 2879 2770
Size: 109 5466
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4582 809
Size: 507 3266 1802
Fragments arranged by size:
3266 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3022 3911 5540
Size: 54 1425 50 275 18 1192 889 1629
Cuts at: 5540 8
Size: 43
Fragments arranged by size:
1629 1425 1192 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3022 1537
Size: 275 1210 4090
Fragments arranged by size:
4090 1210 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2460 2781 3505 1353
Size: 1107 321 724 3423
Fragments arranged by size:
3423 1107 724 321
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4162 4536 4682 387
Size: 1064 138 2573 374 146 1280
Fragments arranged by size:
2573 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2195 2277 3638 4235 5022 5290 1239
Size: 393 563 82 1361 597 787 268 1524
Fragments arranged by size:
1524 1361 787 597 563 393 268 82
EcoRI G'AATT_C
Cuts at: 2884 3262 2884
Size: 378 5197
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2546 2842 2546
Size: 296 5279
FspI TGC'GCA
Cuts at: 4081 4081
Size: 5575
HaeII r_GCGC'y
Cuts at: 1678 4722 1678
Size: 3044 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2360 2392 2597 2629 4216
Size: 32 135 32 788 32 205 32 1587
Cuts at: 4216 4248 4290 4322 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1587 788 205 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2299 2919 3165 669
Size: 988 281 361 620 246 3079
Fragments arranged by size:
3079 988 620 361 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5575
HpaI GTT'AAC
Cuts at: 2299 3165 2299
Size: 866 4709
KpnI G_GTAC'C
Cuts at: 2485 2900 2485
Size: 415 5160
MfeI C'AATT_G
Cuts at: 2598 3174 2598
Size: 576 4999
MluI A'CGCG_T
Cuts at: 5451 5451
Size: 5575
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2666 4689 4873 1305
Size: 1361 2023 184 2007
Fragments arranged by size:
2023 2007 1361 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3753
Fragments arranged by size:
3753 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2315 3533 3892 4051 518
Size: 648 1149 1218 359 159 2042
Fragments arranged by size:
2042 1218 1149 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2991 3668 4816 5061 1606
Size: 1385 677 1148 245 2120
Fragments arranged by size:
2120 1385 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2145 513
Size: 552 939 141 3943
Fragments arranged by size:
3943 939 552 141
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5575
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5575
NotI GC'GGCC_GC
Cuts at: 3022 3022
Size: 5575
NsiI A_TGCA'T
Cuts at: 2133 2133
Size: 5575
NspI r_CATG'y
Cuts at: 1132 1229 2131 2933 4478 1132
Size: 97 902 802 1545 2229
Fragments arranged by size:
2229 1545 902 802 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5575
PciI A'CATG_T
Cuts at: 4474 4474
Size: 5575
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5575
PmeI GTTT'AAAC
Cuts at: 2879 2879
Size: 5575
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3644 3676 5157 5189 3644
Size: 32 1481 32 4030
Fragments arranged by size:
4030 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5575
PsiI TTA'TAA
Cuts at: 2657 2742 3145 2657
Size: 85 403 5087
Fragments arranged by size:
5087 403 85
PstI C_TGCA'G
Cuts at: 830 2337 2634 2927 830
Size: 1507 297 293 3478
Fragments arranged by size:
3478 1507 297 293
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5575
SalI G'TCGA_C
Cuts at: 1936 2917 1936
Size: 981 4594
SbfI CC_TGCA'GG
Cuts at: 2337 2927 2337
Size: 590 4985
ScaI AGT'ACT
Cuts at: 3823 5535 3823
Size: 1712 3863
SexAI A'CCwGG_T
Cuts at: 2851 2851
Size: 5575
SfcI C'TryA_G
Cuts at: 826 1045 2333 2630 2725 2923 4058 4739 4930
Size: 219 1288 297 95 198 1135 681 191
Cuts at: 4930 826
Size: 1471
Fragments arranged by size:
1471 1288 1135 681 297 219 198 191
95
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5575
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5575
SmaI CCC'GGG
Cuts at: 1872 2902 1872
Size: 1030 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2087 3679 4580 4842 820
Size: 197 926 144 1592 901 262 1553
Fragments arranged by size:
1592 1553 926 901 262 197 144
SnaBI TAC'GTA
Cuts at: 493 3366 493
Size: 2873 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5575
SphI G_CATG'C
Cuts at: 1132 2933 1132
Size: 1801 3774
SspI AAT'ATT
Cuts at: 5 52 3499 5
Size: 47 3447 2081
Fragments arranged by size:
3447 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5575
StyI C'CwwG_G
Cuts at: 513 1065 2004 2145 2826 3014 513
Size: 552 939 141 681 188 3074
Fragments arranged by size:
3074 939 681 552 188 141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2190 3602 3619 3772 3957 4402 325
Size: 1255 610 1412 17 153 185 445 1498
Fragments arranged by size:
1498 1412 1255 610 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2229 3821 5533
Size: 274 80 33 51 1443 252 1592 1712
Cuts at: 5533 96
Size: 138
Fragments arranged by size:
1712 1592 1443 274 252 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5535 5567 5535
Size: 32 5543
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5575
XbaI T'CTAG_A
Cuts at: 2407 2911 2407
Size: 504 5071
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5575
XmaI C'CCGG_G
Cuts at: 1870 2900 1870
Size: 1030 4545
XmnI GAAnn'nnTTC
Cuts at: 3704 3704
Size: 5575
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2637 3383 276
Size: 53 83 186 936 1103 746 2468
Fragments arranged by size:
2468 1103 936 746 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BstZ17I DraIII EcoICRI EcoNI EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SfiI SfoI SrfI
SwaI XcmI