Restriction map for
pFN21AE6662
(Circular) (Six-base) MAPSORT of: pFN21AE6662.seq Check: 2491 from: 1 to: 5533
pFN21AE6662 5533 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3343 278
Size: 53 83 186 936 1807 2468
Fragments arranged by size:
2468 1807 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2787 2876 1937
Size: 850 89 4594
Fragments arranged by size:
4594 850 89
Acc65I G'GTAC_C
Cuts at: 2854 2854
Size: 5533
AclI AA'CG_TT
Cuts at: 1563 3660 4033 1563
Size: 2097 373 3063
Fragments arranged by size:
3063 2097 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3596 4980 5248 1632
Size: 1964 1384 268 1917
Fragments arranged by size:
1964 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5336
AflIII A'CryG_T
Cuts at: 1203 4432 5409 1203
Size: 3229 977 1327
Fragments arranged by size:
3229 1327 977
AgeI A'CCGG_T
Cuts at: 4364 4364
Size: 5533
AhdI GACnn_n'nnGTC
Cuts at: 4262 4262
Size: 5533
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5533
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5533
AlwNI CAG_nnn'CTG
Cuts at: 2191 3276 4848 2191
Size: 1085 1572 2876
Fragments arranged by size:
2876 1572 1085
ApaLI G'TGCA_C
Cuts at: 3590 4746 3590
Size: 1156 4377
ApoI r'AATT_y
Cuts at: 1493 2842 3068 3220 5329 1493
Size: 1349 226 152 2109 1697
Fragments arranged by size:
2109 1697 1349 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4087 160
Size: 872 3055 1606
Fragments arranged by size:
3055 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5533
AvaI C'yCGr_G
Cuts at: 1870 1943 2469 2858 1870
Size: 73 526 389 4545
Fragments arranged by size:
4545 526 389 73
BamHI G'GATC_C
Cuts at: 2863 3230 2863
Size: 367 5166
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2474 2854 4309 618
Size: 325 217 627 687 380 1455 1842
Fragments arranged by size:
1842 1455 687 627 380 325 217
BanII G_rGCy'C
Cuts at: 1391 2852 1391
Size: 1461 4072
BbeI G_GCGC'C
Cuts at: 2478 2478
Size: 5533
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4562
BbvCI CC'TCA_GC
Cuts at: 2063 2063
Size: 5533
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3724 3758 1903
Size: 34 1787 34 3678
Fragments arranged by size:
3678 1787 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3425 4635 3425
Size: 1210 4323
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5533
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4144 136
Size: 107 122 71 631 605 2472 1525
Fragments arranged by size:
2472 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5528 5528
Size: 5533
BlpI GC'TnA_GC
Cuts at: 2555 2950 2555
Size: 395 5138
Bme1580I G_kGCm'C
Cuts at: 1790 2798 3594 4750 1790
Size: 1008 796 1156 2573
Fragments arranged by size:
2573 1156 1008 796
BmgBI CAC'GTC
Cuts at: 1318 2094 1318
Size: 776 4757
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3264 4222 442
Size: 416 53 83 399 24 1847 958 1753
Fragments arranged by size:
1847 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5533
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2495 2495
Size: 5533
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2054 2701 4193 1239
Size: 815 647 1492 2579
Fragments arranged by size:
2579 1492 815 647
Bpu10I CC'TnA_GC
Cuts at: 2063 2063
Size: 5533
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2630 3658 4523 4821 2630
Size: 1028 865 298 3342
Fragments arranged by size:
3342 1028 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2408 4196 882
Size: 617 909 1788 2219
Fragments arranged by size:
2219 1788 909 617
BsaAI yAC'GTr
Cuts at: 493 1204 2791 3324 493
Size: 711 1587 533 2702
Fragments arranged by size:
2702 1587 711 533
BsaBI GATnn'nnATC
Cuts at: 5527 5527
Size: 5533
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2010 2042 2475 3340
Size: 53 83 186 936 477 32 433 865
Cuts at: 3340 3722 4357 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 865 635 477 433 382 186
83 53 32
BsaWI w'CCGG_w
Cuts at: 3966 4364 4638 4785 5196 3966
Size: 398 274 147 411 4303
Fragments arranged by size:
4303 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2027 2057 1178
Size: 30 165 30 624 30 4654
Fragments arranged by size:
4654 624 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2288 1186
Size: 1102 4431
BseYI C'CCAG_C
Cuts at: 4736 5232 4736
Size: 496 5037
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2224 1163
Size: 573 488 4472
Fragments arranged by size:
4472 573 488
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2983 3744 4772 1540
Size: 275 186 982 761 1028 2301
Fragments arranged by size:
2301 1028 982 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3594 3679 4750 3594
Size: 85 1071 4377
Fragments arranged by size:
4377 1071 85
BsmI GAATG_Cn'
Cuts at: 3044 3137 3044
Size: 93 5440
BsmBI CGTCTCn'nnnn_
Cuts at: 2100 2100
Size: 5533
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2798 2852 3594 3679 4750 1391
Size: 399 1008 54 742 85 1071 2174
Fragments arranged by size:
2174 1071 1008 742 399 85 54
BspEI T'CCGG_A
Cuts at: 5196 5196
Size: 5533
BspHI T'CATG_A
Cuts at: 3422 5152 3422
Size: 1730 3803
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2888 844
Size: 2044 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3420 1394
Size: 429 1597 3507
Fragments arranged by size:
3507 1597 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2710 4028 4202 65
Size: 1183 1462 1318 174 1396
Fragments arranged by size:
1462 1396 1318 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4177 4364 1700
Size: 110 2367 187 2869
Fragments arranged by size:
2869 2367 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5533
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5533
BssSI C'ACGA_G
Cuts at: 3593 4605 3593
Size: 1012 4521
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5533
BstBI TT'CG_AA
Cuts at: 4337 4337
Size: 5533
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5533
BstYI r'GATC_y
Cuts at: 1972 2863 3230 3615 3632 5084 5170 5182 5528
Size: 891 367 385 17 1452 86 12 346
Cuts at: 5528 1972
Size: 1977
Fragments arranged by size:
1977 1452 891 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4042
Fragments arranged by size:
4042 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2164 487
Size: 683 56 938 3856
Fragments arranged by size:
3856 938 683 56
BtsI GCAGTG_nn'
Cuts at: 2056 3049 3843 3863 2056
Size: 993 794 20 3726
Fragments arranged by size:
3726 993 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5533
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5498
DraI TTT'AAA
Cuts at: 2837 2837
Size: 5533
DraIII CAC_nnn'GTG
Cuts at: 2247 2247
Size: 5533
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4540 809
Size: 507 3224 1802
Fragments arranged by size:
3224 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2457 2735 2980
Size: 54 1425 50 275 18 627 278 245
Cuts at: 2980 3869 5498 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 627 278 275 245 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2980 1537
Size: 275 1168 4090
Fragments arranged by size:
4090 1168 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2144 2406 2486 3463 1353
Size: 791 262 80 977 3423
Fragments arranged by size:
3423 977 791 262 80
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4120 4494 4640 387
Size: 1064 138 2531 374 146 1280
Fragments arranged by size:
2531 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2054 2701 3596 4193 4980 5248 1239
Size: 393 422 647 895 597 787 268 1524
Fragments arranged by size:
1524 895 787 647 597 422 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2195 2195
Size: 5533
EcoO109I rG'GnC_Cy
Cuts at: 2059 2188 2320 2676 2059
Size: 129 132 356 4916
Fragments arranged by size:
4916 356 132 129
EcoRI G'AATT_C
Cuts at: 2842 3220 2842
Size: 378 5155
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2326 2326
Size: 5533
FspI TGC'GCA
Cuts at: 2723 4039 2723
Size: 1316 4217
HaeII r_GCGC'y
Cuts at: 1678 2478 4680 1678
Size: 800 2202 2531
Fragments arranged by size:
2531 2202 800
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2027 2059 4174 4206 4248
Size: 32 135 32 455 32 2115 32 42
Cuts at: 4248 4280 1373
Size: 32 2626
Fragments arranged by size:
2626 2115 455 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2877 3123 669
Size: 988 281 939 246 3079
Fragments arranged by size:
3079 988 939 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5533
HpaI GTT'AAC
Cuts at: 3123 3123
Size: 5533
KasI G'GCGC_C
Cuts at: 2474 2474
Size: 5533
KpnI G_GTAC'C
Cuts at: 2858 2858
Size: 5533
MfeI C'AATT_G
Cuts at: 3132 3132
Size: 5533
MluI A'CGCG_T
Cuts at: 5409 5409
Size: 5533
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4647 4831 1305
Size: 3342 184 2007
Fragments arranged by size:
3342 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2459 10
Size: 54 1425 343 627 3084
Fragments arranged by size:
3084 1425 627 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2690 3491 3850 4009 518
Size: 648 1524 801 359 159 2042
Fragments arranged by size:
2042 1524 801 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2949 3626 4774 5019 1606
Size: 1343 677 1148 245 2120
Fragments arranged by size:
2120 1343 1148 677 245
NarI GG'CG_CC
Cuts at: 2475 2475
Size: 5533
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4042
Fragments arranged by size:
4042 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5533
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5533
NotI GC'GGCC_GC
Cuts at: 2980 2980
Size: 5533
NspI r_CATG'y
Cuts at: 1132 1229 2891 4436 1132
Size: 97 1662 1545 2229
Fragments arranged by size:
2229 1662 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5533
PciI A'CATG_T
Cuts at: 4432 4432
Size: 5533
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5533
PfoI T'CCnGG_A
Cuts at: 2678 2678
Size: 5533
PmeI GTTT'AAAC
Cuts at: 2837 2837
Size: 5533
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3602 3634 5115 5147 3602
Size: 32 1481 32 3988
Fragments arranged by size:
3988 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2320 2676 2320
Size: 356 5177
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5533
PsiI TTA'TAA
Cuts at: 3103 3103
Size: 5533
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2765 2797 2765
Size: 32 5501
PstI C_TGCA'G
Cuts at: 830 2355 2885 830
Size: 1525 530 3478
Fragments arranged by size:
3478 1525 530
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5533
RsrII CG'GwC_CG
Cuts at: 2071 2071
Size: 5533
SalI G'TCGA_C
Cuts at: 1936 2875 1936
Size: 939 4594
SanDI GG'GwC_CC
Cuts at: 2320 2320
Size: 5533
SbfI CC_TGCA'GG
Cuts at: 2885 2885
Size: 5533
ScaI AGT'ACT
Cuts at: 3781 5493 3781
Size: 1712 3821
SfcI C'TryA_G
Cuts at: 826 1045 2227 2351 2881 4016 4697 4888 826
Size: 219 1182 124 530 1135 681 191 1471
Fragments arranged by size:
1471 1182 1135 681 530 219 191 124
SfoI GGC'GCC
Cuts at: 2476 2476
Size: 5533
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5533
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5533
SmaI CCC'GGG
Cuts at: 1872 2860 1872
Size: 988 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2609 3637 4538 4800 820
Size: 197 926 666 1028 901 262 1553
Fragments arranged by size:
1553 1028 926 901 666 262 197
SnaBI TAC'GTA
Cuts at: 493 3324 493
Size: 2831 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5533
SphI G_CATG'C
Cuts at: 1132 2891 1132
Size: 1759 3774
SspI AAT'ATT
Cuts at: 5 52 3457 5
Size: 47 3405 2081
Fragments arranged by size:
3405 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5533
StyI C'CwwG_G
Cuts at: 513 1065 2004 2155 2652 2972 513
Size: 552 939 151 497 320 3074
Fragments arranged by size:
3074 939 552 497 320 151
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2519 3560 3577 3730 3915 4360 325
Size: 1255 939 1041 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1041 939 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3779 5491 96
Size: 274 80 33 51 1443 1802 1712 138
Fragments arranged by size:
1802 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5493 5525 5493
Size: 32 5501
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5533
XbaI T'CTAG_A
Cuts at: 2869 2869
Size: 5533
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2275 2275
Size: 5533
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5533
XmaI C'CCGG_G
Cuts at: 1870 2858 1870
Size: 988 4545
XmnI GAAnn'nnTTC
Cuts at: 3662 3662
Size: 5533
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3341 276
Size: 53 83 186 936 1807 2468
Fragments arranged by size:
2468 1807 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI
PvuI RsrII SalI SanDI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI
NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI
PvuII SacI SacII SapI SexAI SfiI SrfI SwaI