Restriction map for
pFN21AE6656
(Circular) (Six-base) MAPSORT of: pFN21AE6656.seq Check: 2062 from: 1 to: 5482
pFN21AE6656 5482 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3292 278
Size: 53 83 186 936 1756 2468
Fragments arranged by size:
2468 1756 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2825 1937
Size: 888 4594
Acc65I G'GTAC_C
Cuts at: 2803 2803
Size: 5482
AclI AA'CG_TT
Cuts at: 1563 3609 3982 1563
Size: 2046 373 3063
Fragments arranged by size:
3063 2046 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2008 3545 4929 5197 1632
Size: 376 1537 1384 268 1917
Fragments arranged by size:
1917 1537 1384 376 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5285
AflIII A'CryG_T
Cuts at: 1203 4381 5358 1203
Size: 3178 977 1327
Fragments arranged by size:
3178 1327 977
AgeI A'CCGG_T
Cuts at: 4313 4313
Size: 5482
AhdI GACnn_n'nnGTC
Cuts at: 4211 4211
Size: 5482
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5482
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5482
AlwNI CAG_nnn'CTG
Cuts at: 3225 4797 3225
Size: 1572 3910
ApaI G_GGCC'C
Cuts at: 2365 2365
Size: 5482
ApaLI G'TGCA_C
Cuts at: 3539 4695 3539
Size: 1156 4326
ApoI r'AATT_y
Cuts at: 1493 2404 2791 3017 3169 5278 1493
Size: 911 387 226 152 2109 1697
Fragments arranged by size:
2109 1697 911 387 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4036 160
Size: 872 3004 1606
Fragments arranged by size:
3004 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5482
AvaI C'yCGr_G
Cuts at: 1870 1943 2043 2598 2807 1870
Size: 73 100 555 209 4545
Fragments arranged by size:
4545 555 209 100 73
BamHI G'GATC_C
Cuts at: 2812 3179 2812
Size: 367 5115
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2803 4258 618
Size: 325 217 627 1016 1455 1842
Fragments arranged by size:
1842 1455 1016 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2365 2801 1391
Size: 974 436 4072
Fragments arranged by size:
4072 974 436
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2445 928
Size: 971 546 3965
Fragments arranged by size:
3965 971 546
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3673 3707 1903
Size: 34 1736 34 3678
Fragments arranged by size:
3678 1736 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3374 4584 3374
Size: 1210 4272
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5482
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4093 136
Size: 107 122 71 631 605 2421 1525
Fragments arranged by size:
2421 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5477 5477
Size: 5482
BlpI GC'TnA_GC
Cuts at: 2899 2899
Size: 5482
Bme1580I G_kGCm'C
Cuts at: 1790 2365 3543 4699 1790
Size: 575 1178 1156 2573
Fragments arranged by size:
2573 1178 1156 575
BmgBI CAC'GTC
Cuts at: 1318 2635 1318
Size: 1317 4165
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2062 2523 3213
Size: 416 53 83 399 24 645 461 690
Cuts at: 3213 4171 442
Size: 958 1753
Fragments arranged by size:
1753 958 690 645 461 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5482
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2168 2505 2523 4142 1239
Size: 929 337 18 1619 2579
Fragments arranged by size:
2579 1619 929 337 18
Bpu10I CC'TnA_GC
Cuts at: 2159 2159
Size: 5482
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2228 2759 3607 4472 4770 2228
Size: 531 848 865 298 2940
Fragments arranged by size:
2940 865 848 531 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4145 882
Size: 617 2646 2219
Fragments arranged by size:
2646 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3273 493
Size: 711 2069 2702
Fragments arranged by size:
2702 2069 711
BsaBI GATnn'nnATC
Cuts at: 5476 5476
Size: 5482
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2010 3289 3671 4306
Size: 53 83 186 936 477 1279 382 635
Cuts at: 4306 275
Size: 1451
Fragments arranged by size:
1451 1279 936 635 477 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3915 4313 4587 4734 5145 3915
Size: 398 274 147 411 4252
Fragments arranged by size:
4252 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2039 2069 2435 2465 2478
Size: 30 165 30 636 30 366 30 13
Cuts at: 2478 2508 1178
Size: 30 4152
Fragments arranged by size:
4152 636 366 165 30 30 30 30
30 13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2502 2505 2559 1186
Size: 1316 3 54 4109
Fragments arranged by size:
4109 1316 54 3
BseYI C'CCAG_C
Cuts at: 2364 4685 5181 2364
Size: 2321 496 2665
Fragments arranged by size:
2665 2321 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4909
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2932 3693 4721 1540
Size: 275 186 931 761 1028 2301
Fragments arranged by size:
2301 1028 931 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2032 3543 3628 4699 2032
Size: 1511 85 1071 2815
Fragments arranged by size:
2815 1511 1071 85
BsmI GAATG_Cn'
Cuts at: 2993 3086 2993
Size: 93 5389
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2032 2365 2801 3543 3628 4699 1391
Size: 399 242 333 436 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 436 399 333 242 85
BspEI T'CCGG_A
Cuts at: 5145 5145
Size: 5482
BspHI T'CATG_A
Cuts at: 2400 3371 5101 2400
Size: 971 1730 2781
Fragments arranged by size:
2781 1730 971
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2837 844
Size: 1993 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2050 3369 1394
Size: 429 227 1319 3507
Fragments arranged by size:
3507 1319 429 227
BsrDI GCAATG_nn'
Cuts at: 65 1248 3977 4151 65
Size: 1183 2729 174 1396
Fragments arranged by size:
2729 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2466 4126 4313 1700
Size: 110 656 1660 187 2869
Fragments arranged by size:
2869 1660 656 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5482
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5482
BssSI C'ACGA_G
Cuts at: 2723 3542 4554 2723
Size: 819 1012 3651
Fragments arranged by size:
3651 1012 819
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2587 794
Size: 1793 3689
BstBI TT'CG_AA
Cuts at: 4286 4286
Size: 5482
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5482
BstYI r'GATC_y
Cuts at: 1972 2812 3179 3564 3581 5033 5119 5131 5477
Size: 840 367 385 17 1452 86 12 346
Cuts at: 5477 1972
Size: 1977
Fragments arranged by size:
1977 1452 840 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3991
Fragments arranged by size:
3991 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4743
Fragments arranged by size:
4743 683 56
BtsI GCAGTG_nn'
Cuts at: 2998 3792 3812 2998
Size: 794 20 4668
Fragments arranged by size:
4668 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5482
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5447
DraI TTT'AAA
Cuts at: 2786 2786
Size: 5482
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2015 4489 809
Size: 507 699 2474 1802
Fragments arranged by size:
2474 1802 699 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2089 2929 3818
Size: 54 1425 50 275 18 259 840 889
Cuts at: 3818 5447 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 840 275 259 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2929 1537
Size: 275 1117 4090
Fragments arranged by size:
4090 1117 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2088 2663 3412 1353
Size: 735 575 749 3423
Fragments arranged by size:
3423 749 735 575
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4069 4443 4589 387
Size: 1064 138 2480 374 146 1280
Fragments arranged by size:
2480 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2008 2168 2505 2523 3545 4142 4929
Size: 393 376 160 337 18 1022 597 787
Cuts at: 4929 5197 1239
Size: 268 1524
Fragments arranged by size:
1524 1022 787 597 393 376 337 268
160 18
EcoRI G'AATT_C
Cuts at: 2791 3169 2791
Size: 378 5104
FspI TGC'GCA
Cuts at: 3988 3988
Size: 5482
HaeII r_GCGC'y
Cuts at: 1678 2692 4629 1678
Size: 1014 1937 2531
Fragments arranged by size:
2531 1937 1014
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2244 2276 2435 2467 2478
Size: 32 135 32 672 32 159 32 11
Cuts at: 2478 2510 4123 4155 4197 4229 1373
Size: 32 1613 32 42 32 2626
Fragments arranged by size:
2626 1613 672 159 135 42 32 32
32 32 32 32 32 11
HincII GTy'rAC
Cuts at: 669 1657 1938 2826 3072 669
Size: 988 281 888 246 3079
Fragments arranged by size:
3079 988 888 281 246
HindIII A'AGCT_T
Cuts at: 748 2174 748
Size: 1426 4056
HpaI GTT'AAC
Cuts at: 3072 3072
Size: 5482
KpnI G_GTAC'C
Cuts at: 2807 2807
Size: 5482
MfeI C'AATT_G
Cuts at: 3081 3081
Size: 5482
MluI A'CGCG_T
Cuts at: 5358 5358
Size: 5482
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4596 4780 1305
Size: 3291 184 2007
Fragments arranged by size:
3291 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2091 10
Size: 54 1425 343 259 3401
Fragments arranged by size:
3401 1425 343 259 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3440 3799 3958 518
Size: 648 2274 359 159 2042
Fragments arranged by size:
2274 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2898 3575 4723 4968 1606
Size: 1292 677 1148 245 2120
Fragments arranged by size:
2120 1292 1148 677 245
NaeI GCC'GGC
Cuts at: 2468 2468
Size: 5482
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3991
Fragments arranged by size:
3991 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5482
NgoMIV G'CCGG_C
Cuts at: 2466 2466
Size: 5482
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5482
NotI GC'GGCC_GC
Cuts at: 2929 2929
Size: 5482
NspI r_CATG'y
Cuts at: 1132 1229 2840 4385 1132
Size: 97 1611 1545 2229
Fragments arranged by size:
2229 1611 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2699 1348
Size: 1351 4131
PciI A'CATG_T
Cuts at: 4381 4381
Size: 5482
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5482
PfoI T'CCnGG_A
Cuts at: 2539 2539
Size: 5482
PmeI GTTT'AAAC
Cuts at: 2786 2786
Size: 5482
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3551 3583 5064 5096 3551
Size: 32 1481 32 3937
Fragments arranged by size:
3937 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5482
PsiI TTA'TAA
Cuts at: 3052 3052
Size: 5482
PspOMI G'GGCC_C
Cuts at: 2361 2361
Size: 5482
PspXI vC'TCGA_Gb
Cuts at: 2598 2598
Size: 5482
PstI C_TGCA'G
Cuts at: 830 2103 2834 830
Size: 1273 731 3478
Fragments arranged by size:
3478 1273 731
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5482
SalI G'TCGA_C
Cuts at: 1936 2824 1936
Size: 888 4594
SbfI CC_TGCA'GG
Cuts at: 2834 2834
Size: 5482
ScaI AGT'ACT
Cuts at: 3730 5442 3730
Size: 1712 3770
SfcI C'TryA_G
Cuts at: 826 1045 2099 2830 3965 4646 4837 826
Size: 219 1054 731 1135 681 191 1471
Fragments arranged by size:
1471 1135 1054 731 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5482
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5482
SmaI CCC'GGG
Cuts at: 1872 2045 2809 1872
Size: 173 764 4545
Fragments arranged by size:
4545 764 173
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2207 2598 2774 3586 4487 4749
Size: 197 926 264 391 176 812 901 262
Cuts at: 4749 820
Size: 1553
Fragments arranged by size:
1553 926 901 812 391 264 262 197
176
SnaBI TAC'GTA
Cuts at: 493 3273 493
Size: 2780 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5482
SphI G_CATG'C
Cuts at: 1132 2840 1132
Size: 1708 3774
SspI AAT'ATT
Cuts at: 5 52 3406 5
Size: 47 3354 2081
Fragments arranged by size:
3354 2081 47
StuI AGG'CCT
Cuts at: 1517 2682 1517
Size: 1165 4317
StyI C'CwwG_G
Cuts at: 513 1065 2004 2164 2921 513
Size: 552 939 160 757 3074
Fragments arranged by size:
3074 939 757 552 160
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3509 3526 3679 3864 4309 325
Size: 1255 1929 17 153 185 445 1498
Fragments arranged by size:
1929 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2289 3728 5440
Size: 274 80 33 51 1443 312 1439 1712
Cuts at: 5440 96
Size: 138
Fragments arranged by size:
1712 1443 1439 312 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2039 2071 5442 5474 2039
Size: 32 3371 32 2047
Fragments arranged by size:
3371 2047 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2306 2636 1316
Size: 990 330 4162
Fragments arranged by size:
4162 990 330
XbaI T'CTAG_A
Cuts at: 2818 2818
Size: 5482
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2162 2162
Size: 5482
XhoI C'TCGA_G
Cuts at: 1943 2598 1943
Size: 655 4827
XmaI C'CCGG_G
Cuts at: 1870 2043 2807 1870
Size: 173 764 4545
Fragments arranged by size:
4545 764 173
XmnI GAAnn'nnTTC
Cuts at: 3611 3611
Size: 5482
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3290 276
Size: 53 83 186 936 1756 2468
Fragments arranged by size:
2468 1756 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI
PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NarI NruI NsiI PacI PmlI PpuMI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI