Restriction map for
pFN21AE6654
(Circular) (Six-base) MAPSORT of: pFN21AE6654.seq Check: 8339 from: 1 to: 5437
pFN21AE6654 5437 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2558 3247 278
Size: 53 83 186 936 1022 689 2468
Fragments arranged by size:
2468 1022 936 689 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2448 2780 1937
Size: 511 332 4594
Fragments arranged by size:
4594 511 332
Acc65I G'GTAC_C
Cuts at: 2758 2758
Size: 5437
AclI AA'CG_TT
Cuts at: 1563 3564 3937 1563
Size: 2001 373 3063
Fragments arranged by size:
3063 2001 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3500 4884 5152 1632
Size: 1868 1384 268 1917
Fragments arranged by size:
1917 1868 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5240
AflIII A'CryG_T
Cuts at: 1203 4336 5313 1203
Size: 3133 977 1327
Fragments arranged by size:
3133 1327 977
AgeI A'CCGG_T
Cuts at: 4268 4268
Size: 5437
AhdI GACnn_n'nnGTC
Cuts at: 4166 4166
Size: 5437
AleI CACnn'nnGTG
Cuts at: 1166 2546 1166
Size: 1380 4057
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5437
AlwNI CAG_nnn'CTG
Cuts at: 2083 3180 4752 2083
Size: 1097 1572 2768
Fragments arranged by size:
2768 1572 1097
ApaI G_GGCC'C
Cuts at: 2231 2511 2231
Size: 280 5157
ApaLI G'TGCA_C
Cuts at: 3494 4650 3494
Size: 1156 4281
ApoI r'AATT_y
Cuts at: 1493 2746 2972 3124 5233 1493
Size: 1253 226 152 2109 1697
Fragments arranged by size:
2109 1697 1253 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3991 160
Size: 872 2959 1606
Fragments arranged by size:
2959 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5437
AvaI C'yCGr_G
Cuts at: 1870 1943 2158 2376 2504 2762 1870
Size: 73 215 218 128 258 4545
Fragments arranged by size:
4545 258 218 215 128 73
BamHI G'GATC_C
Cuts at: 2767 3134 2767
Size: 367 5070
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2465 2758 4213 618
Size: 325 217 627 678 293 1455 1842
Fragments arranged by size:
1842 1455 678 627 325 293 217
BanII G_rGCy'C
Cuts at: 1391 2231 2505 2511 2756 1391
Size: 840 274 6 245 4072
Fragments arranged by size:
4072 840 274 245 6
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4466
BbvCI CC'TCA_GC
Cuts at: 2634 2634
Size: 5437
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3628 3662 1903
Size: 34 1691 34 3678
Fragments arranged by size:
3678 1691 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3329 4539 3329
Size: 1210 4227
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5437
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4048 136
Size: 107 122 71 631 605 2376 1525
Fragments arranged by size:
2376 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5432 5432
Size: 5437
BlpI GC'TnA_GC
Cuts at: 2854 2854
Size: 5437
Bme1580I G_kGCm'C
Cuts at: 1790 2159 2231 2470 2511 3498 4654 1790
Size: 369 72 239 41 987 1156 2573
Fragments arranged by size:
2573 1156 987 369 239 72 41
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5437
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2203 3168 4126
Size: 416 53 83 399 24 786 965 958
Cuts at: 4126 442
Size: 1753
Fragments arranged by size:
1753 965 958 786 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5437
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2139 2139
Size: 5437
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2303 2392 2596 4097 1239
Size: 1064 89 204 1501 2579
Fragments arranged by size:
2579 1501 1064 204 89
Bpu10I CC'TnA_GC
Cuts at: 2634 2634
Size: 5437
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3562 4427 4725 3562
Size: 865 298 4274
Fragments arranged by size:
4274 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4100 882
Size: 617 2601 2219
Fragments arranged by size:
2601 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2647 3228 493
Size: 711 1443 581 2702
Fragments arranged by size:
2702 1443 711 581
BsaBI GATnn'nnATC
Cuts at: 5431 5431
Size: 5437
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2555 3244 3626 4261
Size: 53 83 186 936 1022 689 382 635
Cuts at: 4261 275
Size: 1451
Fragments arranged by size:
1451 1022 936 689 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3870 4268 4542 4689 5100 3870
Size: 398 274 147 411 4207
Fragments arranged by size:
4207 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5212
Fragments arranged by size:
5212 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5437
BseYI C'CCAG_C
Cuts at: 2215 2421 4640 5136 2215
Size: 206 2219 496 2516
Fragments arranged by size:
2516 2219 496 206
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2069 1163
Size: 573 333 4531
Fragments arranged by size:
4531 573 333
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2887 3648 4676 1540
Size: 275 186 886 761 1028 2301
Fragments arranged by size:
2301 1028 886 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2420 2505 2646 3498 3583 4654 2420
Size: 85 141 852 85 1071 3203
Fragments arranged by size:
3203 1071 852 141 85 85
BsmI GAATG_Cn'
Cuts at: 2533 2948 3041 2533
Size: 415 93 4929
Fragments arranged by size:
4929 415 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2159 2231 2420 2470 2505 2511 2646
Size: 399 369 72 189 50 35 6 135
Cuts at: 2646 2756 3498 3583 4654 1391
Size: 110 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 369 189 135 110
85 72 50 35 6
BspEI T'CCGG_A
Cuts at: 5100 5100
Size: 5437
BspHI T'CATG_A
Cuts at: 3326 5056 3326
Size: 1730 3707
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2247 2334 2792 844
Size: 1403 87 458 3489
Fragments arranged by size:
3489 1403 458 87
BsrBI CCG'CTC
Cuts at: 1394 1823 2209 3324 1394
Size: 429 386 1115 3507
Fragments arranged by size:
3507 1115 429 386
BsrDI GCAATG_nn'
Cuts at: 65 1248 3932 4106 65
Size: 1183 2684 174 1396
Fragments arranged by size:
2684 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2710 4081 4268 1700
Size: 110 900 1371 187 2869
Fragments arranged by size:
2869 1371 900 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5437
BssHII G'CGCG_C
Cuts at: 1925 2097 1925
Size: 172 5265
BssSI C'ACGA_G
Cuts at: 3497 4509 3497
Size: 1012 4425
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5437
BstBI TT'CG_AA
Cuts at: 4241 4241
Size: 5437
BstEII G'GTnAC_C
Cuts at: 1184 2038 2287 1184
Size: 854 249 4334
Fragments arranged by size:
4334 854 249
BstXI CCAn_nnnn'nTGG
Cuts at: 2491 2491
Size: 5437
BstYI r'GATC_y
Cuts at: 1972 2131 2309 2517 2767 3134 3519 3536 4988
Size: 159 178 208 250 367 385 17 1452
Cuts at: 4988 5074 5086 5432 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 250 208 178
159 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2232 2559 2694 513
Size: 552 939 228 327 135 3256
Fragments arranged by size:
3256 939 552 327 228 135
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4698
Fragments arranged by size:
4698 683 56
BtsI GCAGTG_nn'
Cuts at: 2953 3747 3767 2953
Size: 794 20 4623
Fragments arranged by size:
4623 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5437
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5402
DraI TTT'AAA
Cuts at: 2741 2741
Size: 5437
DraIII CAC_nnn'GTG
Cuts at: 2650 2650
Size: 5437
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2144 4444 809
Size: 507 828 2300 1802
Fragments arranged by size:
2300 1802 828 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2358 2884 3773
Size: 54 1425 50 275 18 528 526 889
Cuts at: 3773 5402 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 528 526 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2884 1537
Size: 275 1072 4090
Fragments arranged by size:
4090 1072 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3367 1353
Size: 2014 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2144 4024 4398 4544 387
Size: 1064 138 555 1880 374 146 1280
Fragments arranged by size:
1880 1280 1064 555 374 146 138
EcoICRI GAG'CTC
Cuts at: 2503 2503
Size: 5437
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2303 2392 2596 3500 4097 4884 5152
Size: 393 671 89 204 904 597 787 268
Cuts at: 5152 1239
Size: 1524
Fragments arranged by size:
1524 904 787 671 597 393 268 204
89
EcoO109I rG'GnC_Cy
Cuts at: 2228 2508 2228
Size: 280 5157
EcoRI G'AATT_C
Cuts at: 2746 3124 2746
Size: 378 5059
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2317 2317
Size: 5437
FspI TGC'GCA
Cuts at: 3943 3943
Size: 5437
HaeII r_GCGC'y
Cuts at: 1678 2084 2216 4584 1678
Size: 406 132 2368 2531
Fragments arranged by size:
2531 2368 406 132
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4078 4110 4152 4184 1373
Size: 32 135 32 2506 32 42 32 2626
Fragments arranged by size:
2626 2506 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2781 3027 669
Size: 988 281 843 246 3079
Fragments arranged by size:
3079 988 843 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5437
HpaI GTT'AAC
Cuts at: 3027 3027
Size: 5437
KpnI G_GTAC'C
Cuts at: 2762 2762
Size: 5437
MfeI C'AATT_G
Cuts at: 3036 3036
Size: 5437
MluI A'CGCG_T
Cuts at: 5313 5313
Size: 5437
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4551 4735 1305
Size: 3246 184 2007
Fragments arranged by size:
3246 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2360 10
Size: 54 1425 343 528 3087
Fragments arranged by size:
3087 1425 528 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2342 2546 3395 3754 3913 518
Size: 648 1176 204 849 359 159 2042
Fragments arranged by size:
2042 1176 849 648 359 204 159
MspA1I CmG'CkG
Cuts at: 1606 2258 2425 2853 3530 4678 4923 1606
Size: 652 167 428 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 652 428 245 167
NcoI C'CATG_G
Cuts at: 513 1065 2004 2559 513
Size: 552 939 555 3391
Fragments arranged by size:
3391 939 555 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5437
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5437
NotI GC'GGCC_GC
Cuts at: 2884 2884
Size: 5437
NspI r_CATG'y
Cuts at: 1132 1229 2479 2795 4340 1132
Size: 97 1250 316 1545 2229
Fragments arranged by size:
2229 1545 1250 316 97
PasI CC'CwG_GG
Cuts at: 1348 2387 1348
Size: 1039 4398
PciI A'CATG_T
Cuts at: 4336 4336
Size: 5437
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5437
PfoI T'CCnGG_A
Cuts at: 2280 2280
Size: 5437
PmeI GTTT'AAAC
Cuts at: 2741 2741
Size: 5437
PmlI CAC'GTG
Cuts at: 2647 2647
Size: 5437
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3506 3538 5019 5051 3506
Size: 32 1481 32 3892
Fragments arranged by size:
3892 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5437
PsiI TTA'TAA
Cuts at: 3007 3007
Size: 5437
PspOMI G'GGCC_C
Cuts at: 2227 2507 2227
Size: 280 5157
PstI C_TGCA'G
Cuts at: 830 2274 2789 830
Size: 1444 515 3478
Fragments arranged by size:
3478 1444 515
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5437
SacI G_AGCT'C
Cuts at: 2505 2505
Size: 5437
SalI G'TCGA_C
Cuts at: 1936 2779 1936
Size: 843 4594
SbfI CC_TGCA'GG
Cuts at: 2789 2789
Size: 5437
ScaI AGT'ACT
Cuts at: 3685 5397 3685
Size: 1712 3725
SfcI C'TryA_G
Cuts at: 826 1045 2270 2785 3920 4601 4792 826
Size: 219 1225 515 1135 681 191 1471
Fragments arranged by size:
1471 1225 1135 681 515 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5437
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5437
SmaI CCC'GGG
Cuts at: 1872 2160 2378 2764 1872
Size: 288 218 386 4545
Fragments arranged by size:
4545 386 288 218
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3541 4442 4704 820
Size: 197 926 1598 901 262 1553
Fragments arranged by size:
1598 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3228 493
Size: 2735 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5437
SphI G_CATG'C
Cuts at: 1132 2795 1132
Size: 1663 3774
SspI AAT'ATT
Cuts at: 5 52 3361 5
Size: 47 3309 2081
Fragments arranged by size:
3309 2081 47
StuI AGG'CCT
Cuts at: 1517 2300 1517
Size: 783 4654
StyI C'CwwG_G
Cuts at: 513 1065 2004 2292 2460 2484 2559 2876 513
Size: 552 939 288 168 24 75 317 3074
Fragments arranged by size:
3074 939 552 317 288 168 75 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2177 2694 3464 3481 3634 3819 4264
Size: 1255 597 517 770 17 153 185 445
Cuts at: 4264 325
Size: 1498
Fragments arranged by size:
1498 1255 770 597 517 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3683 5395 96
Size: 274 80 33 51 1443 1706 1712 138
Fragments arranged by size:
1712 1706 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5397 5429 5397
Size: 32 5405
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5437
XbaI T'CTAG_A
Cuts at: 2773 2773
Size: 5437
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2467 2710 2467
Size: 243 5194
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5437
XmaI C'CCGG_G
Cuts at: 1870 2158 2376 2762 1870
Size: 288 218 386 4545
Fragments arranged by size:
4545 386 288 218
XmnI GAAnn'nnTTC
Cuts at: 3566 3566
Size: 5437
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2556 3245 276
Size: 53 83 186 936 1022 689 2468
Fragments arranged by size:
2468 1022 936 689 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PstI PvuI SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI
PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI