Restriction map for
pFN21AE6650
(Circular) (Six-base) MAPSORT of: pFN21AE6650.seq Check: 7998 from: 1 to: 5407
pFN21AE6650 5407 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2312 2419 2312
Size: 107 5300
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2691 3217 278
Size: 53 83 186 936 1155 526 2468
Fragments arranged by size:
2468 1155 936 526 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2750 1937
Size: 813 4594
Acc65I G'GTAC_C
Cuts at: 2728 2728
Size: 5407
AclI AA'CG_TT
Cuts at: 1563 3534 3907 1563
Size: 1971 373 3063
Fragments arranged by size:
3063 1971 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2066 2521 3470 4854 5122 1632
Size: 434 455 949 1384 268 1917
Fragments arranged by size:
1917 1384 949 455 434 268
AfeI AGC'GCT
Cuts at: 2043 2043
Size: 5407
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5210
AflIII A'CryG_T
Cuts at: 1203 4306 5283 1203
Size: 3103 977 1327
Fragments arranged by size:
3103 1327 977
AgeI A'CCGG_T
Cuts at: 4238 4238
Size: 5407
AhdI GACnn_n'nnGTC
Cuts at: 4136 4136
Size: 5407
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5407
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2141 2669 1184
Size: 957 528 3922
Fragments arranged by size:
3922 957 528
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2543 2575 2543
Size: 32 5375
AlwNI CAG_nnn'CTG
Cuts at: 2209 2398 2683 3150 4722 2209
Size: 189 285 467 1572 2894
Fragments arranged by size:
2894 1572 467 285 189
ApaI G_GGCC'C
Cuts at: 2627 2627
Size: 5407
ApaLI G'TGCA_C
Cuts at: 3464 4620 3464
Size: 1156 4251
ApoI r'AATT_y
Cuts at: 1493 2716 2942 3094 5203 1493
Size: 1223 226 152 2109 1697
Fragments arranged by size:
2109 1697 1223 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3961 160
Size: 872 2929 1606
Fragments arranged by size:
2929 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5407
AvaI C'yCGr_G
Cuts at: 1870 1943 2468 2627 2732 1870
Size: 73 525 159 105 4545
Fragments arranged by size:
4545 525 159 105 73
BamHI G'GATC_C
Cuts at: 2737 3104 2737
Size: 367 5040
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2728 4183 618
Size: 325 217 627 941 1455 1842
Fragments arranged by size:
1842 1455 941 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2559 2627 2726 1391
Size: 1168 68 99 4072
Fragments arranged by size:
4072 1168 99 68
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4436
BbvCI CC'TCA_GC
Cuts at: 2360 2360
Size: 5407
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3598 3632 1903
Size: 34 1661 34 3678
Fragments arranged by size:
3678 1661 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3299 4509 3299
Size: 1210 4197
BclI T'GATC_A
Cuts at: 1548 2604 1548
Size: 1056 4351
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2178 4018 136
Size: 107 122 71 631 605 506 1840 1525
Fragments arranged by size:
1840 1525 631 605 506 122 107 71
BglII A'GATC_T
Cuts at: 5402 5402
Size: 5407
BlpI GC'TnA_GC
Cuts at: 2597 2824 2597
Size: 227 5180
Bme1580I G_kGCm'C
Cuts at: 1790 2580 2627 3468 4624 1790
Size: 790 47 841 1156 2573
Fragments arranged by size:
2573 1156 841 790 47
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5407
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3138 4096 442
Size: 416 53 83 399 24 1721 958 1753
Fragments arranged by size:
1753 1721 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5407
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2114 2195 2231 2258 4067 1239
Size: 875 81 36 27 1809 2579
Fragments arranged by size:
2579 1809 875 81 36 27
Bpu10I CC'TnA_GC
Cuts at: 2109 2360 2109
Size: 251 5156
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3532 4397 4695 3532
Size: 865 298 4244
Fragments arranged by size:
4244 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4070 882
Size: 617 2571 2219
Fragments arranged by size:
2571 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2276 2377 2479 2584 3198 493
Size: 711 1072 101 102 105 614 2702
Fragments arranged by size:
2702 1072 711 614 105 102 101
BsaBI GATnn'nnATC
Cuts at: 5401 5401
Size: 5407
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2286 2493 2688 3214
Size: 53 83 186 936 753 207 195 526
Cuts at: 3214 3596 4231 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 753 635 526 382 207 195
186 83 53
BsaWI w'CCGG_w
Cuts at: 3840 4238 4512 4659 5070 3840
Size: 398 274 147 411 4177
Fragments arranged by size:
4177 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2543 2573 1178
Size: 30 165 30 1140 30 4012
Fragments arranged by size:
4012 1140 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5407
BseYI C'CCAG_C
Cuts at: 2056 2649 4610 5106 2056
Size: 593 1961 496 2357
Fragments arranged by size:
2357 1961 593 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2222 2539 1163
Size: 573 486 317 4031
Fragments arranged by size:
4031 573 486 317
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2857 3618 4646 1540
Size: 275 186 856 761 1028 2301
Fragments arranged by size:
2301 1028 856 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2559 3468 3553 4624 2559
Size: 909 85 1071 3342
Fragments arranged by size:
3342 1071 909 85
BsmI GAATG_Cn'
Cuts at: 2918 3011 2918
Size: 93 5314
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2559 2580 2627 2726 3468 3553 4624
Size: 399 769 21 47 99 742 85 1071
Cuts at: 4624 1391
Size: 2174
Fragments arranged by size:
2174 1071 769 742 399 99 85 47
21
BspEI T'CCGG_A
Cuts at: 5070 5070
Size: 5407
BspHI T'CATG_A
Cuts at: 2328 2547 3296 5026 2328
Size: 219 749 1730 2709
Fragments arranged by size:
2709 1730 749 219
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2238 2312 2419 2643 2762 844
Size: 1394 74 107 224 119 3489
Fragments arranged by size:
3489 1394 224 119 107 74
BsrBI CCG'CTC
Cuts at: 1394 1823 2220 3294 1394
Size: 429 397 1074 3507
Fragments arranged by size:
3507 1074 429 397
BsrDI GCAATG_nn'
Cuts at: 65 1248 3902 4076 65
Size: 1183 2654 174 1396
Fragments arranged by size:
2654 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4051 4238 1700
Size: 110 2241 187 2869
Fragments arranged by size:
2869 2241 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5407
BssHII G'CGCG_C
Cuts at: 1925 2320 2638 2640 2642 1925
Size: 395 318 2 2 4690
Fragments arranged by size:
4690 395 318 2 2
BssSI C'ACGA_G
Cuts at: 3467 4479 3467
Size: 1012 4395
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5407
BstBI TT'CG_AA
Cuts at: 4211 4211
Size: 5407
BstEII G'GTnAC_C
Cuts at: 1184 2587 1184
Size: 1403 4004
BstXI CCAn_nnnn'nTGG
Cuts at: 2416 2416
Size: 5407
BstYI r'GATC_y
Cuts at: 1972 2737 3104 3489 3506 4958 5044 5056 5402
Size: 765 367 385 17 1452 86 12 346
Cuts at: 5402 1972
Size: 1977
Fragments arranged by size:
1977 1452 765 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2079 2263 513
Size: 552 1014 184 3657
Fragments arranged by size:
3657 1014 552 184
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4668
Fragments arranged by size:
4668 683 56
BtsI GCAGTG_nn'
Cuts at: 2923 3717 3737 2923
Size: 794 20 4593
Fragments arranged by size:
4593 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5407
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5372
DraI TTT'AAA
Cuts at: 2711 2711
Size: 5407
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4414 809
Size: 507 3098 1802
Fragments arranged by size:
3098 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2854 3743 5372
Size: 54 1425 50 275 18 1024 889 1629
Cuts at: 5372 8
Size: 43
Fragments arranged by size:
1629 1425 1024 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2854 1537
Size: 275 1042 4090
Fragments arranged by size:
4090 1042 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3337 1353
Size: 1984 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3994 4368 4514 387
Size: 1064 138 2405 374 146 1280
Fragments arranged by size:
2405 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2557 2557
Size: 5407
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2066 2114 2195 2231 2258 2521 3470
Size: 393 434 48 81 36 27 263 949
Cuts at: 3470 4067 4854 5122 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 949 787 597 434 393 268 263
81 48 36 27
EcoO109I rG'GnC_Cy
Cuts at: 2395 2624 2632 2395
Size: 229 8 5170
Fragments arranged by size:
5170 229 8
EcoRI G'AATT_C
Cuts at: 2716 3094 2716
Size: 378 5029
FspI TGC'GCA
Cuts at: 2165 3913 2165
Size: 1748 3659
HaeII r_GCGC'y
Cuts at: 1678 2045 2173 2246 4554 1678
Size: 367 128 73 2308 2531
Fragments arranged by size:
2531 2308 367 128 73
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4048 4080 4122 4154 1373
Size: 32 135 32 2476 32 42 32 2626
Fragments arranged by size:
2626 2476 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2149 2751 2997 669
Size: 988 281 211 602 246 3079
Fragments arranged by size:
3079 988 602 281 246 211
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5407
HpaI GTT'AAC
Cuts at: 2997 2997
Size: 5407
KpnI G_GTAC'C
Cuts at: 2732 2732
Size: 5407
MfeI C'AATT_G
Cuts at: 3006 3006
Size: 5407
MluI A'CGCG_T
Cuts at: 5283 5283
Size: 5407
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2307 2433 4521 4705 1305
Size: 1002 126 2088 184 2007
Fragments arranged by size:
2088 2007 1002 184 126
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3585
Fragments arranged by size:
3585 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2546 3365 3724 3883 518
Size: 648 843 537 819 359 159 2042
Fragments arranged by size:
2042 843 819 648 537 359 159
MspA1I CmG'CkG
Cuts at: 1606 2265 2313 2653 2680 2823 3500 4648 4893
Size: 659 48 340 27 143 677 1148 245
Cuts at: 4893 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 659 340 245 143 48
27
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4855
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5407
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5407
NotI GC'GGCC_GC
Cuts at: 2854 2854
Size: 5407
NspI r_CATG'y
Cuts at: 1132 1229 2765 4310 1132
Size: 97 1536 1545 2229
Fragments arranged by size:
2229 1545 1536 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5407
PciI A'CATG_T
Cuts at: 4306 4306
Size: 5407
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2398 1108
Size: 1290 4117
PfoI T'CCnGG_A
Cuts at: 2208 2208
Size: 5407
PmeI GTTT'AAAC
Cuts at: 2711 2711
Size: 5407
PmlI CAC'GTG
Cuts at: 2479 2479
Size: 5407
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2543 2575 3476 3508 4989 5021 2543
Size: 32 901 32 1481 32 2929
Fragments arranged by size:
2929 1481 901 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2632 2632
Size: 5407
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5407
PsiI TTA'TAA
Cuts at: 2977 2977
Size: 5407
PspOMI G'GGCC_C
Cuts at: 2623 2623
Size: 5407
PstI C_TGCA'G
Cuts at: 830 2298 2679 2759 830
Size: 1468 381 80 3478
Fragments arranged by size:
3478 1468 381 80
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5407
PvuII CAG'CTG
Cuts at: 2313 2653 2680 2313
Size: 340 27 5040
Fragments arranged by size:
5040 340 27
SacI G_AGCT'C
Cuts at: 2559 2559
Size: 5407
SacII CC_GC'GG
Cuts at: 2266 2266
Size: 5407
SalI G'TCGA_C
Cuts at: 1936 2749 1936
Size: 813 4594
SbfI CC_TGCA'GG
Cuts at: 2759 2759
Size: 5407
ScaI AGT'ACT
Cuts at: 3655 5367 3655
Size: 1712 3695
SfcI C'TryA_G
Cuts at: 826 1045 2294 2675 2755 3890 4571 4762 826
Size: 219 1249 381 80 1135 681 191 1471
Fragments arranged by size:
1471 1249 1135 681 381 219 191 80
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5407
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5407
SmaI CCC'GGG
Cuts at: 1872 2734 1872
Size: 862 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3511 4412 4674 820
Size: 197 926 1568 901 262 1553
Fragments arranged by size:
1568 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3198 493
Size: 2705 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5407
SphI G_CATG'C
Cuts at: 1132 2765 1132
Size: 1633 3774
SspI AAT'ATT
Cuts at: 5 52 3331 5
Size: 47 3279 2081
Fragments arranged by size:
3279 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5407
StyI C'CwwG_G
Cuts at: 513 1065 2846 513
Size: 552 1781 3074
Fragments arranged by size:
3074 1781 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2663 3434 3451 3604 3789 4234 325
Size: 1255 1083 771 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1083 771 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2352 3653 5365
Size: 274 80 33 51 1443 375 1301 1712
Cuts at: 5365 96
Size: 138
Fragments arranged by size:
1712 1443 1301 375 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5367 5399 5367
Size: 32 5375
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5407
XbaI T'CTAG_A
Cuts at: 2743 2743
Size: 5407
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5407
XmaI C'CCGG_G
Cuts at: 1870 2732 1870
Size: 862 4545
XmnI GAAnn'nnTTC
Cuts at: 3536 3536
Size: 5407
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2689 3215 276
Size: 53 83 186 936 1155 526 2468
Fragments arranged by size:
2468 1155 936 526 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SacII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI
BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI
PsrI RsrII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI