Restriction map for
pFN21AE6641
(Circular) (Six-base) MAPSORT of: pFN21AE6641.seq Check: 6830 from: 1 to: 5482
pFN21AE6641 5482 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3292 278
Size: 53 83 186 936 1756 2468
Fragments arranged by size:
2468 1756 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2825 1937
Size: 888 4594
Acc65I G'GTAC_C
Cuts at: 2803 2803
Size: 5482
AclI AA'CG_TT
Cuts at: 1563 3609 3982 1563
Size: 2046 373 3063
Fragments arranged by size:
3063 2046 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3545 4929 5197 1632
Size: 1913 1384 268 1917
Fragments arranged by size:
1917 1913 1384 268
AfeI AGC'GCT
Cuts at: 2330 2330
Size: 5482
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5285
AflIII A'CryG_T
Cuts at: 1203 4381 5358 1203
Size: 3178 977 1327
Fragments arranged by size:
3178 1327 977
AgeI A'CCGG_T
Cuts at: 4313 4313
Size: 5482
AhdI GACnn_n'nnGTC
Cuts at: 2145 4211 2145
Size: 2066 3416
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5482
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2639 1184
Size: 1455 4027
AlwNI CAG_nnn'CTG
Cuts at: 2396 3225 4797 2396
Size: 829 1572 3081
Fragments arranged by size:
3081 1572 829
ApaLI G'TGCA_C
Cuts at: 3539 4695 3539
Size: 1156 4326
ApoI r'AATT_y
Cuts at: 1493 2682 2791 3017 3169 5278 1493
Size: 1189 109 226 152 2109 1697
Fragments arranged by size:
2109 1697 1189 226 152 109
AseI AT'TA_AT
Cuts at: 160 1032 4036 160
Size: 872 3004 1606
Fragments arranged by size:
3004 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5482
AvaI C'yCGr_G
Cuts at: 1870 1943 2807 1870
Size: 73 864 4545
Fragments arranged by size:
4545 864 73
BamHI G'GATC_C
Cuts at: 2812 3179 2812
Size: 367 5115
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2803 4258 618
Size: 325 217 627 1016 1455 1842
Fragments arranged by size:
1842 1455 1016 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2081 2801 1391
Size: 690 720 4072
Fragments arranged by size:
4072 720 690
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2509 928
Size: 971 610 3901
Fragments arranged by size:
3901 971 610
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2432 2466 3673 3707 1903
Size: 34 495 34 1207 34 3678
Fragments arranged by size:
3678 1207 495 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2396 3374 4584 2396
Size: 978 1210 3294
Fragments arranged by size:
3294 1210 978
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5482
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4093 136
Size: 107 122 71 631 605 2421 1525
Fragments arranged by size:
2421 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5477 5477
Size: 5482
BlpI GC'TnA_GC
Cuts at: 2899 2899
Size: 5482
Bme1580I G_kGCm'C
Cuts at: 1790 3543 4699 1790
Size: 1753 1156 2573
Fragments arranged by size:
2573 1753 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5482
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2352 2384 3213
Size: 416 53 83 399 24 935 32 829
Cuts at: 3213 4171 442
Size: 958 1753
Fragments arranged by size:
1753 958 935 829 416 399 83 53
32 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5482
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4142 1239
Size: 2903 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3607 4472 4770 3607
Size: 865 298 4319
Fragments arranged by size:
4319 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2538 4145 882
Size: 617 1039 1607 2219
Fragments arranged by size:
2219 1607 1039 617
BsaAI yAC'GTr
Cuts at: 493 1204 3273 493
Size: 711 2069 2702
Fragments arranged by size:
2702 2069 711
BsaBI GATnn'nnATC
Cuts at: 5476 5476
Size: 5482
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2141 3289 3671 4306
Size: 53 83 186 936 608 1148 382 635
Cuts at: 4306 275
Size: 1451
Fragments arranged by size:
1451 1148 936 635 608 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3915 4313 4587 4734 5145 3915
Size: 398 274 147 411 4252
Fragments arranged by size:
4252 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2610 2640 1178
Size: 30 165 30 1207 30 4020
Fragments arranged by size:
4020 1207 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2737 1186
Size: 1551 3931
BseYI C'CCAG_C
Cuts at: 4685 5181 4685
Size: 496 4986
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4909
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2932 3693 4721 1540
Size: 275 186 931 761 1028 2301
Fragments arranged by size:
2301 1028 931 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2081 2593 2628 3543 3628 4699 2081
Size: 512 35 915 85 1071 2864
Fragments arranged by size:
2864 1071 915 512 85 35
BsmI GAATG_Cn'
Cuts at: 2189 2993 3086 2189
Size: 804 93 4585
Fragments arranged by size:
4585 804 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2132 2132
Size: 5482
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2081 2593 2628 2801 3543 3628 4699
Size: 399 291 512 35 173 742 85 1071
Cuts at: 4699 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 512 399 291 173 85
35
BspEI T'CCGG_A
Cuts at: 5145 5145
Size: 5482
BspHI T'CATG_A
Cuts at: 3371 5101 3371
Size: 1730 3752
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2837 844
Size: 1993 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3369 1394
Size: 429 1546 3507
Fragments arranged by size:
3507 1546 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3977 4151 65
Size: 1183 2729 174 1396
Fragments arranged by size:
2729 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4126 4313 1700
Size: 110 2316 187 2869
Fragments arranged by size:
2869 2316 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5482
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5482
BssSI C'ACGA_G
Cuts at: 3542 4554 3542
Size: 1012 4470
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2352 794
Size: 1558 3924
BstBI TT'CG_AA
Cuts at: 4286 4286
Size: 5482
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5482
BstXI CCAn_nnnn'nTGG
Cuts at: 2342 2342
Size: 5482
BstYI r'GATC_y
Cuts at: 1972 2812 3179 3564 3581 5033 5119 5131 5477
Size: 840 367 385 17 1452 86 12 346
Cuts at: 5477 1972
Size: 1977
Fragments arranged by size:
1977 1452 840 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2058 2458 513
Size: 552 939 54 400 3537
Fragments arranged by size:
3537 939 552 400 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4743
Fragments arranged by size:
4743 683 56
BtsI GCAGTG_nn'
Cuts at: 2248 2998 3792 3812 2248
Size: 750 794 20 3918
Fragments arranged by size:
3918 794 750 20
ClaI AT'CG_AT
Cuts at: 1554 2161 1554
Size: 607 4875
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5447
DraI TTT'AAA
Cuts at: 2786 2786
Size: 5482
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4489 809
Size: 507 3173 1802
Fragments arranged by size:
3173 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2055 2929
Size: 54 1425 50 275 18 177 48 874
Cuts at: 2929 3818 5447 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 874 275 177 54 50
48 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2929 1537
Size: 275 1117 4090
Fragments arranged by size:
4090 1117 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3412 1353
Size: 2059 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4069 4443 4589 387
Size: 1064 138 2480 374 146 1280
Fragments arranged by size:
2480 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2079 2079
Size: 5482
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3545 4142 4929 5197 1239
Size: 393 1913 597 787 268 1524
Fragments arranged by size:
1913 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2222 2222
Size: 5482
EcoO109I rG'GnC_Cy
Cuts at: 2710 2710
Size: 5482
EcoRI G'AATT_C
Cuts at: 2791 3169 2791
Size: 378 5104
FspI TGC'GCA
Cuts at: 3988 3988
Size: 5482
HaeII r_GCGC'y
Cuts at: 1678 2332 4629 1678
Size: 654 2297 2531
Fragments arranged by size:
2531 2297 654
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2131 2163 4123 4155 4197
Size: 32 135 32 559 32 1960 32 42
Cuts at: 4197 4229 1373
Size: 32 2626
Fragments arranged by size:
2626 1960 559 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2826 3072 669
Size: 988 281 888 246 3079
Fragments arranged by size:
3079 988 888 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5482
HpaI GTT'AAC
Cuts at: 3072 3072
Size: 5482
KpnI G_GTAC'C
Cuts at: 2807 2807
Size: 5482
MfeI C'AATT_G
Cuts at: 3081 3081
Size: 5482
MluI A'CGCG_T
Cuts at: 5358 5358
Size: 5482
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2294 4596 4780 1305
Size: 989 2302 184 2007
Fragments arranged by size:
2302 2007 989 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3660
Fragments arranged by size:
3660 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2340 2516 3440 3799 3958 518
Size: 648 1174 176 924 359 159 2042
Fragments arranged by size:
2042 1174 924 648 359 176 159
MspA1I CmG'CkG
Cuts at: 1606 2060 2367 2898 3575 4723 4968 1606
Size: 454 307 531 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 531 454 307 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3991
Fragments arranged by size:
3991 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5482
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5482
NotI GC'GGCC_GC
Cuts at: 2929 2929
Size: 5482
NspI r_CATG'y
Cuts at: 1132 1229 2176 2350 2840 4385 1132
Size: 97 947 174 490 1545 2229
Fragments arranged by size:
2229 1545 947 490 174 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5482
PciI A'CATG_T
Cuts at: 4381 4381
Size: 5482
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2185 2482 1108
Size: 1077 297 4108
Fragments arranged by size:
4108 1077 297
PmeI GTTT'AAAC
Cuts at: 2786 2786
Size: 5482
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3551 3583 5064 5096 3551
Size: 32 1481 32 3937
Fragments arranged by size:
3937 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5482
PsiI TTA'TAA
Cuts at: 3052 3052
Size: 5482
PstI C_TGCA'G
Cuts at: 830 2834 830
Size: 2004 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5482
SacI G_AGCT'C
Cuts at: 2081 2081
Size: 5482
SacII CC_GC'GG
Cuts at: 2061 2061
Size: 5482
SalI G'TCGA_C
Cuts at: 1936 2824 1936
Size: 888 4594
SbfI CC_TGCA'GG
Cuts at: 2834 2834
Size: 5482
ScaI AGT'ACT
Cuts at: 3730 5442 3730
Size: 1712 3770
SfcI C'TryA_G
Cuts at: 826 1045 2830 3965 4646 4837 826
Size: 219 1785 1135 681 191 1471
Fragments arranged by size:
1785 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5482
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5482
SmaI CCC'GGG
Cuts at: 1872 2809 1872
Size: 937 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3586 4487 4749 820
Size: 197 926 1643 901 262 1553
Fragments arranged by size:
1643 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3273 493
Size: 2780 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5482
SphI G_CATG'C
Cuts at: 1132 2840 1132
Size: 1708 3774
SspI AAT'ATT
Cuts at: 5 52 3406 5
Size: 47 3354 2081
Fragments arranged by size:
3354 2081 47
StuI AGG'CCT
Cuts at: 1517 2228 1517
Size: 711 4771
StyI C'CwwG_G
Cuts at: 513 1065 2004 2179 2287 2921 513
Size: 552 939 175 108 634 3074
Fragments arranged by size:
3074 939 634 552 175 108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3509 3526 3679 3864 4309 325
Size: 1255 1929 17 153 185 445 1498
Fragments arranged by size:
1929 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3728 5440 96
Size: 274 80 33 51 1443 1751 1712 138
Fragments arranged by size:
1751 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5442 5474 5442
Size: 32 5450
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5482
XbaI T'CTAG_A
Cuts at: 2818 2818
Size: 5482
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5482
XmaI C'CCGG_G
Cuts at: 1870 2807 1870
Size: 937 4545
XmnI GAAnn'nnTTC
Cuts at: 3611 3611
Size: 5482
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3290 276
Size: 53 83 186 936 1756 2468
Fragments arranged by size:
2468 1756 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI SacI SacII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI