Restriction map for
pFN21AE6634
(Circular) (Six-base) MAPSORT of: pFN21AE6634.seq Check: 6725 from: 1 to: 5353
pFN21AE6634 5353 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2167 2167
Size: 5353
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3163 278
Size: 53 83 186 936 1627 2468
Fragments arranged by size:
2468 1627 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2696 1937
Size: 759 4594
Acc65I G'GTAC_C
Cuts at: 2674 2674
Size: 5353
AclI AA'CG_TT
Cuts at: 1563 3480 3853 1563
Size: 1917 373 3063
Fragments arranged by size:
3063 1917 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2210 2381 3416 4800 5068 1632
Size: 578 171 1035 1384 268 1917
Fragments arranged by size:
1917 1384 1035 578 268 171
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5156
AflIII A'CryG_T
Cuts at: 1203 4252 5229 1203
Size: 3049 977 1327
Fragments arranged by size:
3049 1327 977
AgeI A'CCGG_T
Cuts at: 4184 4184
Size: 5353
AhdI GACnn_n'nnGTC
Cuts at: 4082 4082
Size: 5353
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5353
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5353
AlwNI CAG_nnn'CTG
Cuts at: 2161 2221 2341 2350 3096 4668 2161
Size: 60 120 9 746 1572 2846
Fragments arranged by size:
2846 1572 746 120 60 9
ApaI G_GGCC'C
Cuts at: 2398 2398
Size: 5353
ApaLI G'TGCA_C
Cuts at: 3410 4566 3410
Size: 1156 4197
ApoI r'AATT_y
Cuts at: 1493 2662 2888 3040 5149 1493
Size: 1169 226 152 2109 1697
Fragments arranged by size:
2109 1697 1169 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3907 160
Size: 872 2875 1606
Fragments arranged by size:
2875 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5353
AvaI C'yCGr_G
Cuts at: 1870 1943 2678 1870
Size: 73 735 4545
Fragments arranged by size:
4545 735 73
BamHI G'GATC_C
Cuts at: 2683 3050 2683
Size: 367 4986
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2104 2179 2503 2674 4129
Size: 325 217 627 317 75 324 171 1455
Cuts at: 4129 618
Size: 1842
Fragments arranged by size:
1842 1455 627 325 324 317 217 171
75
BanII G_rGCy'C
Cuts at: 1391 2398 2672 1391
Size: 1007 274 4072
Fragments arranged by size:
4072 1007 274
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4382
BbvCI CC'TCA_GC
Cuts at: 2645 2645
Size: 5353
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2031 2065 3544 3578 1903
Size: 34 50 34 10 34 1479 34 3678
Fragments arranged by size:
3678 1479 50 34 34 34 34 10
BciVI GTATCCnnnnn_n'
Cuts at: 3245 4455 3245
Size: 1210 4143
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5353
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3964 136
Size: 107 122 71 631 605 337 1955 1525
Fragments arranged by size:
1955 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5348 5348
Size: 5353
BlpI GC'TnA_GC
Cuts at: 2770 2770
Size: 5353
Bme1580I G_kGCm'C
Cuts at: 1790 2109 2398 3414 4570 1790
Size: 319 289 1016 1156 2573
Fragments arranged by size:
2573 1156 1016 319 289
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5353
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3084 4042 442
Size: 416 53 83 399 24 1667 958 1753
Fragments arranged by size:
1753 1667 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5353
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2450 4013 1239
Size: 1211 1563 2579
Fragments arranged by size:
2579 1563 1211
Bpu10I CC'TnA_GC
Cuts at: 2645 2645
Size: 5353
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3478 4343 4641 3478
Size: 865 298 4190
Fragments arranged by size:
4190 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4016 882
Size: 617 2517 2219
Fragments arranged by size:
2517 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3144 493
Size: 711 1940 2702
Fragments arranged by size:
2702 1940 711
BsaBI GATnn'nnATC
Cuts at: 5347 5347
Size: 5353
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3160 3542 4177 275
Size: 53 83 186 936 1627 382 635 1451
Fragments arranged by size:
1627 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3786 4184 4458 4605 5016 3786
Size: 398 274 147 411 4123
Fragments arranged by size:
4123 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2463 2493 1178
Size: 30 165 30 1060 30 4038
Fragments arranged by size:
4038 1060 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2258 2390 1186
Size: 1072 132 4149
Fragments arranged by size:
4149 1072 132
BseYI C'CCAG_C
Cuts at: 4556 5052 4556
Size: 496 4857
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4780
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2803 3564 4592 1540
Size: 275 186 802 761 1028 2301
Fragments arranged by size:
2301 1028 802 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2450 3414 3499 4570 2450
Size: 964 85 1071 3233
Fragments arranged by size:
3233 1071 964 85
BsmI GAATG_Cn'
Cuts at: 2864 2957 2864
Size: 93 5260
BsmBI CGTCTCn'nnnn_
Cuts at: 2093 2466 2093
Size: 373 4980
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2109 2398 2450 2672 3414 3499 4570
Size: 399 319 289 52 222 742 85 1071
Cuts at: 4570 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 319 289 222 85
52
BspEI T'CCGG_A
Cuts at: 5016 5016
Size: 5353
BspHI T'CATG_A
Cuts at: 3242 4972 3242
Size: 1730 3623
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2167 2708 844
Size: 1323 541 3489
Fragments arranged by size:
3489 1323 541
BsrBI CCG'CTC
Cuts at: 1394 1823 3240 1394
Size: 429 1417 3507
Fragments arranged by size:
3507 1417 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3848 4022 65
Size: 1183 2600 174 1396
Fragments arranged by size:
2600 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2619 3997 4184 1700
Size: 110 809 1378 187 2869
Fragments arranged by size:
2869 1378 809 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5353
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5353
BssSI C'ACGA_G
Cuts at: 3413 4425 3413
Size: 1012 4341
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5353
BstBI TT'CG_AA
Cuts at: 2065 4157 2065
Size: 2092 3261
BstEII G'GTnAC_C
Cuts at: 1184 2479 1184
Size: 1295 4058
BstYI r'GATC_y
Cuts at: 1972 2436 2539 2683 3050 3435 3452 4904 4990
Size: 464 103 144 367 385 17 1452 86
Cuts at: 4990 5002 5348 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 464 385 367 346 144 103
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2274 2508 2274
Size: 234 5119
BtgI C'CryG_G
Cuts at: 513 1065 2484 513
Size: 552 1419 3382
Fragments arranged by size:
3382 1419 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2305 487
Size: 683 56 1079 3535
Fragments arranged by size:
3535 1079 683 56
BtsI GCAGTG_nn'
Cuts at: 2869 3663 3683 2869
Size: 794 20 4539
Fragments arranged by size:
4539 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5353
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5318
DraI TTT'AAA
Cuts at: 2657 2657
Size: 5353
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4360 809
Size: 507 3044 1802
Fragments arranged by size:
3044 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2223 2800 3689
Size: 54 1425 50 275 18 393 577 889
Cuts at: 3689 5318 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 577 393 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2800 1537
Size: 275 988 4090
Fragments arranged by size:
4090 988 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2282 3283 1353
Size: 929 1001 3423
Fragments arranged by size:
3423 1001 929
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3940 4314 4460 387
Size: 1064 138 2351 374 146 1280
Fragments arranged by size:
2351 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2210 2381 2450 3416 4013 4800 5068
Size: 393 578 171 69 966 597 787 268
Cuts at: 5068 1239
Size: 1524
Fragments arranged by size:
1524 966 787 597 578 393 268 171
69
EcoO109I rG'GnC_Cy
Cuts at: 2278 2278
Size: 5353
EcoRI G'AATT_C
Cuts at: 2662 3040 2662
Size: 378 4975
FspI TGC'GCA
Cuts at: 3859 3859
Size: 5353
HaeII r_GCGC'y
Cuts at: 1678 4500 1678
Size: 2822 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3994 4026 4068 4100 1373
Size: 32 135 32 2422 32 42 32 2626
Fragments arranged by size:
2626 2422 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2697 2943 669
Size: 988 281 759 246 3079
Fragments arranged by size:
3079 988 759 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5353
HpaI GTT'AAC
Cuts at: 2943 2943
Size: 5353
KpnI G_GTAC'C
Cuts at: 2678 2678
Size: 5353
MfeI C'AATT_G
Cuts at: 2952 2952
Size: 5353
MluI A'CGCG_T
Cuts at: 5229 5229
Size: 5353
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4467 4651 1305
Size: 3162 184 2007
Fragments arranged by size:
3162 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2225 10
Size: 54 1425 343 393 3138
Fragments arranged by size:
3138 1425 393 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3311 3670 3829 518
Size: 648 2145 359 159 2042
Fragments arranged by size:
2145 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2386 2453 2769 3446 4594 4839 1606
Size: 780 67 316 677 1148 245 2120
Fragments arranged by size:
2120 1148 780 677 316 245 67
NaeI GCC'GGC
Cuts at: 2621 2621
Size: 5353
NcoI C'CATG_G
Cuts at: 513 1065 2484 513
Size: 552 1419 3382
Fragments arranged by size:
3382 1419 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5353
NgoMIV G'CCGG_C
Cuts at: 2619 2619
Size: 5353
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5353
NotI GC'GGCC_GC
Cuts at: 2800 2800
Size: 5353
NspI r_CATG'y
Cuts at: 1132 1229 2711 4256 1132
Size: 97 1482 1545 2229
Fragments arranged by size:
2229 1545 1482 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5353
PciI A'CATG_T
Cuts at: 4252 4252
Size: 5353
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2221 1108
Size: 1113 4240
PmeI GTTT'AAAC
Cuts at: 2657 2657
Size: 5353
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3422 3454 4935 4967 3422
Size: 32 1481 32 3808
Fragments arranged by size:
3808 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2278 2278
Size: 5353
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5353
PsiI TTA'TAA
Cuts at: 2923 2923
Size: 5353
PspOMI G'GGCC_C
Cuts at: 2394 2394
Size: 5353
PstI C_TGCA'G
Cuts at: 830 2346 2705 830
Size: 1516 359 3478
Fragments arranged by size:
3478 1516 359
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5353
PvuII CAG'CTG
Cuts at: 2386 2386
Size: 5353
SalI G'TCGA_C
Cuts at: 1936 2695 1936
Size: 759 4594
SbfI CC_TGCA'GG
Cuts at: 2705 2705
Size: 5353
ScaI AGT'ACT
Cuts at: 3601 5313 3601
Size: 1712 3641
SfcI C'TryA_G
Cuts at: 826 1045 2342 2441 2701 3836 4517 4708 826
Size: 219 1297 99 260 1135 681 191 1471
Fragments arranged by size:
1471 1297 1135 681 260 219 191 99
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5353
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5353
SmaI CCC'GGG
Cuts at: 1872 2680 1872
Size: 808 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3457 4358 4620 820
Size: 197 926 1514 901 262 1553
Fragments arranged by size:
1553 1514 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3144 493
Size: 2651 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5353
SphI G_CATG'C
Cuts at: 1132 2711 1132
Size: 1579 3774
SspI AAT'ATT
Cuts at: 5 52 3277 5
Size: 47 3225 2081
Fragments arranged by size:
3225 2081 47
StuI AGG'CCT
Cuts at: 1517 2597 1517
Size: 1080 4273
StyI C'CwwG_G
Cuts at: 513 1065 2484 2792 513
Size: 552 1419 308 3074
Fragments arranged by size:
3074 1419 552 308
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2650 3380 3397 3550 3735 4180 325
Size: 1255 1070 730 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1070 730 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3599 5311 96
Size: 274 80 33 51 1443 1622 1712 138
Fragments arranged by size:
1712 1622 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2463 2495 5313 5345 2463
Size: 32 2818 32 2471
Fragments arranged by size:
2818 2471 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5353
XbaI T'CTAG_A
Cuts at: 2239 2689 2239
Size: 450 4903
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5353
XmaI C'CCGG_G
Cuts at: 1870 2678 1870
Size: 808 4545
XmnI GAAnn'nnTTC
Cuts at: 3482 3482
Size: 5353
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3161 276
Size: 53 83 186 936 1627 2468
Fragments arranged by size:
2468 1627 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PfoI
PmlI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI