Restriction map for
pFN21AE6631
(Circular) (Six-base) MAPSORT of: pFN21AE6631.seq Check: 2712 from: 1 to: 5653
pFN21AE6631 5653 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2354 2480 2354
Size: 126 5527
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3463 278
Size: 53 83 186 936 1927 2468
Fragments arranged by size:
2468 1927 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2488 2996 1937
Size: 551 508 4594
Fragments arranged by size:
4594 551 508
Acc65I G'GTAC_C
Cuts at: 2019 2974 2019
Size: 955 4698
AclI AA'CG_TT
Cuts at: 1563 3780 4153 1563
Size: 2217 373 3063
Fragments arranged by size:
3063 2217 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2415 3716 5100 5368 1632
Size: 783 1301 1384 268 1917
Fragments arranged by size:
1917 1384 1301 783 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5456
AflIII A'CryG_T
Cuts at: 1203 4552 5529 1203
Size: 3349 977 1327
Fragments arranged by size:
3349 1327 977
AgeI A'CCGG_T
Cuts at: 4484 4484
Size: 5653
AhdI GACnn_n'nnGTC
Cuts at: 4382 4382
Size: 5653
AleI CACnn'nnGTG
Cuts at: 1166 2087 2198 2728 2888 1166
Size: 921 111 530 160 3931
Fragments arranged by size:
3931 921 530 160 111
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5653
AlwNI CAG_nnn'CTG
Cuts at: 3396 4968 3396
Size: 1572 4081
ApaI G_GGCC'C
Cuts at: 2339 2339
Size: 5653
ApaLI G'TGCA_C
Cuts at: 2173 3710 4866 2173
Size: 1537 1156 2960
Fragments arranged by size:
2960 1537 1156
ApoI r'AATT_y
Cuts at: 1493 2565 2759 2962 3188 3340 5449 1493
Size: 1072 194 203 226 152 2109 1697
Fragments arranged by size:
2109 1697 1072 226 203 194 152
AseI AT'TA_AT
Cuts at: 160 1032 2445 4207 160
Size: 872 1413 1762 1606
Fragments arranged by size:
1762 1606 1413 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5653
AvaI C'yCGr_G
Cuts at: 1870 1943 2196 2580 2978 1870
Size: 73 253 384 398 4545
Fragments arranged by size:
4545 398 384 253 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2685 2718 2685
Size: 33 5620
BamHI G'GATC_C
Cuts at: 2257 2983 3350 2257
Size: 726 367 4560
Fragments arranged by size:
4560 726 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2019 2250 2974 4429 618
Size: 325 217 627 232 231 724 1455 1842
Fragments arranged by size:
1842 1455 724 627 325 232 231 217
BanII G_rGCy'C
Cuts at: 1391 2339 2972 1391
Size: 948 633 4072
Fragments arranged by size:
4072 948 633
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2556 928
Size: 971 657 4025
Fragments arranged by size:
4025 971 657
BbvCI CC'TCA_GC
Cuts at: 2740 2752 2740
Size: 12 5641
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2591 2625 3844 3878 1903
Size: 34 654 34 1219 34 3678
Fragments arranged by size:
3678 1219 654 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3545 4755 3545
Size: 1210 4443
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5653
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4264 136
Size: 107 122 71 631 605 2592 1525
Fragments arranged by size:
2592 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5648 5648
Size: 5653
BlpI GC'TnA_GC
Cuts at: 3070 3070
Size: 5653
Bme1580I G_kGCm'C
Cuts at: 1790 2094 2177 2339 3714 4870 1790
Size: 304 83 162 1375 1156 2573
Fragments arranged by size:
2573 1375 1156 304 162 83
BmgBI CAC'GTC
Cuts at: 1318 2623 1318
Size: 1305 4348
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3384 4342 442
Size: 416 53 83 399 24 1967 958 1753
Fragments arranged by size:
1967 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5653
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4313 1239
Size: 3074 2579
Bpu10I CC'TnA_GC
Cuts at: 2740 2752 2740
Size: 12 5641
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3778 4643 4941 3778
Size: 865 298 4490
Fragments arranged by size:
4490 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4316 882
Size: 617 2817 2219
Fragments arranged by size:
2817 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3444 493
Size: 711 2240 2702
Fragments arranged by size:
2702 2240 711
BsaBI GATnn'nnATC
Cuts at: 5647 5647
Size: 5653
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3460 3842 4477 275
Size: 53 83 186 936 1927 382 635 1451
Fragments arranged by size:
1927 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4086 4484 4758 4905 5316 4086
Size: 398 274 147 411 4423
Fragments arranged by size:
4423 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2079 2109 1178
Size: 30 165 30 676 30 4722
Fragments arranged by size:
4722 676 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5653
BseYI C'CCAG_C
Cuts at: 2234 2905 4856 5352 2234
Size: 671 1951 496 2535
Fragments arranged by size:
2535 1951 671 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2375 2626 1163
Size: 573 639 251 4190
Fragments arranged by size:
4190 639 573 251
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3103 3864 4892 1540
Size: 275 186 1102 761 1028 2301
Fragments arranged by size:
2301 1102 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2177 2952 3714 3799 4870 2177
Size: 775 762 85 1071 2960
Fragments arranged by size:
2960 1071 775 762 85
BsmI GAATG_Cn'
Cuts at: 3164 3257 3164
Size: 93 5560
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2094 2177 2339 2952 2972 3714 3799
Size: 399 304 83 162 613 20 742 85
Cuts at: 3799 4870 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 613 399 304 162 85
83 20
BspEI T'CCGG_A
Cuts at: 5316 5316
Size: 5653
BspHI T'CATG_A
Cuts at: 3542 5272 3542
Size: 1730 3923
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2354 2480 3008 844
Size: 1510 126 528 3489
Fragments arranged by size:
3489 1510 528 126
BsrBI CCG'CTC
Cuts at: 1394 1823 3540 1394
Size: 429 1717 3507
Fragments arranged by size:
3507 1717 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4148 4322 65
Size: 1183 2900 174 1396
Fragments arranged by size:
2900 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4297 4484 1700
Size: 110 2487 187 2869
Fragments arranged by size:
2869 2487 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5653
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5653
BssSI C'ACGA_G
Cuts at: 2045 3713 4725 2045
Size: 1668 1012 2973
Fragments arranged by size:
2973 1668 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5653
BstBI TT'CG_AA
Cuts at: 4457 4457
Size: 5653
BstEII G'GTnAC_C
Cuts at: 1184 2191 2653 1184
Size: 1007 462 4184
Fragments arranged by size:
4184 1007 462
BstXI CCAn_nnnn'nTGG
Cuts at: 2128 2623 2905 2128
Size: 495 282 4876
Fragments arranged by size:
4876 495 282
BstYI r'GATC_y
Cuts at: 1972 2257 2307 2983 3350 3735 3752 5204 5290
Size: 285 50 676 367 385 17 1452 86
Cuts at: 5290 5302 5648 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 676 385 367 346 285 86
50 17 12
Bsu36I CC'TnA_GG
Cuts at: 2168 2168
Size: 5653
BtgI C'CryG_G
Cuts at: 513 1065 2004 2634 2706 513
Size: 552 939 630 72 3460
Fragments arranged by size:
3460 939 630 552 72
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4914
Fragments arranged by size:
4914 683 56
BtsI GCAGTG_nn'
Cuts at: 2614 2723 2735 3169 3963 3983 2614
Size: 109 12 434 794 20 4284
Fragments arranged by size:
4284 794 434 109 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5653
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5618
DraI TTT'AAA
Cuts at: 2957 2957
Size: 5653
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2189 4660 809
Size: 507 873 2471 1802
Fragments arranged by size:
2471 1802 873 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2625 3100 3989
Size: 54 1425 50 275 18 795 475 889
Cuts at: 3989 5618 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 795 475 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3100 1537
Size: 275 1288 4090
Fragments arranged by size:
4090 1288 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3583 1353
Size: 2230 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4240 4614 4760 387
Size: 1064 138 2651 374 146 1280
Fragments arranged by size:
2651 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2415 3716 4313 5100 5368 1239
Size: 393 783 1301 597 787 268 1524
Fragments arranged by size:
1524 1301 787 783 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2335 2336 2335
Size: 1 5652
EcoRI G'AATT_C
Cuts at: 2759 2962 3340 2759
Size: 203 378 5072
Fragments arranged by size:
5072 378 203
EcoRV GAT'ATC
Cuts at: 2437 2437
Size: 5653
FspI TGC'GCA
Cuts at: 2099 4159 2099
Size: 2060 3593
HaeII r_GCGC'y
Cuts at: 1678 2163 4800 1678
Size: 485 2637 2531
Fragments arranged by size:
2637 2531 485
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2637 2669 2817 2849 4294
Size: 32 135 32 1065 32 148 32 1445
Cuts at: 4294 4326 4368 4400 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1445 1065 148 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2997 3243 669
Size: 988 281 1059 246 3079
Fragments arranged by size:
3079 1059 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5653
HpaI GTT'AAC
Cuts at: 3243 3243
Size: 5653
KpnI G_GTAC'C
Cuts at: 2023 2978 2023
Size: 955 4698
MfeI C'AATT_G
Cuts at: 3252 3252
Size: 5653
MluI A'CGCG_T
Cuts at: 5529 5529
Size: 5653
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2922 4767 4951 1305
Size: 1617 1845 184 2007
Fragments arranged by size:
2007 1845 1617 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3831
Fragments arranged by size:
3831 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2087 2198 2728 2871 2888 2916 3611
Size: 648 921 111 530 143 17 28 695
Cuts at: 3611 3970 4129 518
Size: 359 159 2042
Fragments arranged by size:
2042 921 695 648 530 359 159 143
111 28 17
MspA1I CmG'CkG
Cuts at: 1606 2103 3069 3746 4894 5139 1606
Size: 497 966 677 1148 245 2120
Fragments arranged by size:
2120 1148 966 677 497 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4162
Fragments arranged by size:
4162 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5653
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5653
NotI GC'GGCC_GC
Cuts at: 3100 3100
Size: 5653
NspI r_CATG'y
Cuts at: 1132 1229 3011 4556 1132
Size: 97 1782 1545 2229
Fragments arranged by size:
2229 1782 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5653
PciI A'CATG_T
Cuts at: 4552 4552
Size: 5653
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2317 1108
Size: 1209 4444
PmeI GTTT'AAAC
Cuts at: 2957 2957
Size: 5653
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3722 3754 5235 5267 3722
Size: 32 1481 32 4108
Fragments arranged by size:
4108 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5653
PsiI TTA'TAA
Cuts at: 3223 3223
Size: 5653
PspOMI G'GGCC_C
Cuts at: 2335 2335
Size: 5653
PstI C_TGCA'G
Cuts at: 830 2730 3005 830
Size: 1900 275 3478
Fragments arranged by size:
3478 1900 275
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5653
RsrII CG'GwC_CG
Cuts at: 2143 2143
Size: 5653
SalI G'TCGA_C
Cuts at: 1936 2995 1936
Size: 1059 4594
SbfI CC_TGCA'GG
Cuts at: 3005 3005
Size: 5653
ScaI AGT'ACT
Cuts at: 3901 5613 3901
Size: 1712 3941
SfcI C'TryA_G
Cuts at: 826 1045 2726 3001 4136 4817 5008 826
Size: 219 1681 275 1135 681 191 1471
Fragments arranged by size:
1681 1471 1135 681 275 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5653
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5653
SmaI CCC'GGG
Cuts at: 1872 2198 2582 2980 1872
Size: 326 384 398 4545
Fragments arranged by size:
4545 398 384 326
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3757 4658 4920 820
Size: 197 926 1814 901 262 1553
Fragments arranged by size:
1814 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3444 493
Size: 2951 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5653
SphI G_CATG'C
Cuts at: 1132 3011 1132
Size: 1879 3774
SspI AAT'ATT
Cuts at: 5 52 2461 3577 5
Size: 47 2409 1116 2081
Fragments arranged by size:
2409 2081 1116 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5653
StyI C'CwwG_G
Cuts at: 513 1065 2004 2688 3092 513
Size: 552 939 684 404 3074
Fragments arranged by size:
3074 939 684 552 404
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2300 2385 3680 3697 3850 4035 4480
Size: 1255 720 85 1295 17 153 185 445
Cuts at: 4480 325
Size: 1498
Fragments arranged by size:
1498 1295 1255 720 445 185 153 85
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2295 3899 5611
Size: 274 80 33 51 1443 318 1604 1712
Cuts at: 5611 96
Size: 138
Fragments arranged by size:
1712 1604 1443 318 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2079 2111 2588 2620 5613 5645 2079
Size: 32 477 32 2993 32 2087
Fragments arranged by size:
2993 2087 477 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5653
XbaI T'CTAG_A
Cuts at: 2989 2989
Size: 5653
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5653
XmaI C'CCGG_G
Cuts at: 1870 2196 2580 2978 1870
Size: 326 384 398 4545
Fragments arranged by size:
4545 398 384 326
XmnI GAAnn'nnTTC
Cuts at: 3782 3782
Size: 5653
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3461 276
Size: 53 83 186 936 1927 2468
Fragments arranged by size:
2468 1927 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI RsrII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BfrBI BplI BsiWI
BsmBI BstZ17I DraIII EcoICRI EcoNI FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspXI PsrI PvuII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI