Restriction map for
pFN21AE6625
(Circular) (Six-base) MAPSORT of: pFN21AE6625.seq Check: 8614 from: 1 to: 5707
pFN21AE6625 5707 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2014 3517 278
Size: 53 83 186 936 478 1503 2468
Fragments arranged by size:
2468 1503 936 478 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2068 3050 1937
Size: 131 982 4594
Fragments arranged by size:
4594 982 131
Acc65I G'GTAC_C
Cuts at: 3028 3028
Size: 5707
AclI AA'CG_TT
Cuts at: 1563 3834 4207 1563
Size: 2271 373 3063
Fragments arranged by size:
3063 2271 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2049 2076 2153 3770 5154 5422 1632
Size: 417 27 77 1617 1384 268 1917
Fragments arranged by size:
1917 1617 1384 417 268 77 27
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5510
AflIII A'CryG_T
Cuts at: 1203 2390 4606 5583 1203
Size: 1187 2216 977 1327
Fragments arranged by size:
2216 1327 1187 977
AgeI A'CCGG_T
Cuts at: 4538 4538
Size: 5707
AhdI GACnn_n'nnGTC
Cuts at: 4436 4436
Size: 5707
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5707
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5707
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2936 2968 2936
Size: 32 5675
AlwNI CAG_nnn'CTG
Cuts at: 2027 2825 3450 5022 2027
Size: 798 625 1572 2712
Fragments arranged by size:
2712 1572 798 625
ApaLI G'TGCA_C
Cuts at: 3764 4920 3764
Size: 1156 4551
ApoI r'AATT_y
Cuts at: 1493 2914 3016 3242 3394 5503 1493
Size: 1421 102 226 152 2109 1697
Fragments arranged by size:
2109 1697 1421 226 152 102
AseI AT'TA_AT
Cuts at: 160 1032 2963 4261 160
Size: 872 1931 1298 1606
Fragments arranged by size:
1931 1606 1298 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5707
AvaI C'yCGr_G
Cuts at: 1870 1943 2080 2403 3032 1870
Size: 73 137 323 629 4545
Fragments arranged by size:
4545 629 323 137 73
BamHI G'GATC_C
Cuts at: 3037 3404 3037
Size: 367 5340
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3028 4483 618
Size: 325 217 627 1241 1455 1842
Fragments arranged by size:
1842 1455 1241 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3026 1391
Size: 1635 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2203 2661 928
Size: 971 304 458 3974
Fragments arranged by size:
3974 971 458 304
BbvCI CC'TCA_GC
Cuts at: 2940 2940
Size: 5707
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3898 3932 1903
Size: 34 1961 34 3678
Fragments arranged by size:
3678 1961 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3599 4809 3599
Size: 1210 4497
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5707
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4318 136
Size: 107 122 71 631 605 2646 1525
Fragments arranged by size:
2646 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2150 2164 5702 2150
Size: 14 3538 2155
Fragments arranged by size:
3538 2155 14
BlpI GC'TnA_GC
Cuts at: 3124 3124
Size: 5707
Bme1580I G_kGCm'C
Cuts at: 1790 3768 4924 1790
Size: 1978 1156 2573
Fragments arranged by size:
2573 1978 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5707
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2829 3438 4396
Size: 416 53 83 399 24 1412 609 958
Cuts at: 4396 442
Size: 1753
Fragments arranged by size:
1753 1412 958 609 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5707
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2221 4367 1239
Size: 982 2146 2579
Fragments arranged by size:
2579 2146 982
Bpu10I CC'TnA_GC
Cuts at: 2940 2940
Size: 5707
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3832 4697 4995 3832
Size: 865 298 4544
Fragments arranged by size:
4544 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2406 4370 882
Size: 617 907 1964 2219
Fragments arranged by size:
2219 1964 907 617
BsaAI yAC'GTr
Cuts at: 493 1204 3498 493
Size: 711 2294 2702
Fragments arranged by size:
2702 2294 711
BsaBI GATnn'nnATC
Cuts at: 5701 5701
Size: 5707
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2011 3514 3896 4531
Size: 53 83 186 936 478 1503 382 635
Cuts at: 4531 275
Size: 1451
Fragments arranged by size:
1503 1451 936 635 478 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4140 4538 4812 4959 5370 4140
Size: 398 274 147 411 4477
Fragments arranged by size:
4477 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2328 2358 1178
Size: 30 165 30 925 30 4527
Fragments arranged by size:
4527 925 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2068 1186
Size: 882 4825
BseYI C'CCAG_C
Cuts at: 4910 5406 4910
Size: 496 5211
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5134
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3157 3918 4946 1540
Size: 275 186 1156 761 1028 2301
Fragments arranged by size:
2301 1156 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3768 3853 4924 3768
Size: 85 1071 4551
Fragments arranged by size:
4551 1071 85
BsmI GAATG_Cn'
Cuts at: 3218 3311 3218
Size: 93 5614
BsmBI CGTCTCn'nnnn_
Cuts at: 2348 2348
Size: 5707
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3026 3768 3853 4924 1391
Size: 399 1236 742 85 1071 2174
Fragments arranged by size:
2174 1236 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5370 5370
Size: 5707
BspHI T'CATG_A
Cuts at: 3596 5326 3596
Size: 1730 3977
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3062 844
Size: 2218 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3594 1394
Size: 429 1771 3507
Fragments arranged by size:
3507 1771 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4202 4376 65
Size: 1183 2954 174 1396
Fragments arranged by size:
2954 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4351 4538 1700
Size: 110 2541 187 2869
Fragments arranged by size:
2869 2541 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5707
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5707
BssSI C'ACGA_G
Cuts at: 3767 4779 3767
Size: 1012 4695
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5707
BstBI TT'CG_AA
Cuts at: 4511 4511
Size: 5707
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5707
BstYI r'GATC_y
Cuts at: 1972 2150 2164 2854 3037 3404 3789 3806 5258
Size: 178 14 690 183 367 385 17 1452
Cuts at: 5258 5344 5356 5702 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 690 385 367 346 183 178
86 17 14 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4216
Fragments arranged by size:
4216 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4968
Fragments arranged by size:
4968 683 56
BtsI GCAGTG_nn'
Cuts at: 2371 3223 4017 4037 2371
Size: 852 794 20 4041
Fragments arranged by size:
4041 852 794 20
ClaI AT'CG_AT
Cuts at: 1554 2209 1554
Size: 655 5052
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5672
DraI TTT'AAA
Cuts at: 3011 3011
Size: 5707
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4714 809
Size: 507 3398 1802
Fragments arranged by size:
3398 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3003 3154 4043
Size: 54 1425 50 275 18 1173 151 889
Cuts at: 4043 5672 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1173 889 275 151 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3154 1537
Size: 275 1342 4090
Fragments arranged by size:
4090 1342 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2230 3637 1353
Size: 877 1407 3423
Fragments arranged by size:
3423 1407 877
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4294 4668 4814 387
Size: 1064 138 2705 374 146 1280
Fragments arranged by size:
2705 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2049 2076 2153 2221 3770 4367 5154
Size: 393 417 27 77 68 1549 597 787
Cuts at: 5154 5422 1239
Size: 268 1524
Fragments arranged by size:
1549 1524 787 597 417 393 268 77
68 27
EcoRI G'AATT_C
Cuts at: 3016 3394 3016
Size: 378 5329
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2736 2736
Size: 5707
FspI TGC'GCA
Cuts at: 4213 4213
Size: 5707
HaeII r_GCGC'y
Cuts at: 1678 4854 1678
Size: 3176 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2328 2360 4348 4380 4422
Size: 32 135 32 756 32 1988 32 42
Cuts at: 4422 4454 1373
Size: 32 2626
Fragments arranged by size:
2626 1988 756 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3051 3297 669
Size: 988 281 1113 246 3079
Fragments arranged by size:
3079 1113 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5707
HpaI GTT'AAC
Cuts at: 3297 3297
Size: 5707
KpnI G_GTAC'C
Cuts at: 3032 3032
Size: 5707
MfeI C'AATT_G
Cuts at: 3306 3306
Size: 5707
MluI A'CGCG_T
Cuts at: 2390 5583 2390
Size: 3193 2514
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2998 4821 5005 1305
Size: 1693 1823 184 2007
Fragments arranged by size:
2007 1823 1693 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3885
Fragments arranged by size:
3885 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2187 3665 4024 4183 518
Size: 648 1021 1478 359 159 2042
Fragments arranged by size:
2042 1478 1021 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2543 2569 3123 3800 4948 5193 1606
Size: 937 26 554 677 1148 245 2120
Fragments arranged by size:
2120 1148 937 677 554 245 26
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4216
Fragments arranged by size:
4216 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5707
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5707
NotI GC'GGCC_GC
Cuts at: 3154 3154
Size: 5707
NspI r_CATG'y
Cuts at: 1132 1229 3065 4610 1132
Size: 97 1836 1545 2229
Fragments arranged by size:
2229 1836 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5707
PciI A'CATG_T
Cuts at: 4606 4606
Size: 5707
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5707
PmeI GTTT'AAAC
Cuts at: 3011 3011
Size: 5707
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3776 3808 5289 5321 3776
Size: 32 1481 32 4162
Fragments arranged by size:
4162 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2014 1613
Size: 401 5306
PsiI TTA'TAA
Cuts at: 3277 3277
Size: 5707
PspXI vC'TCGA_Gb
Cuts at: 2080 2080
Size: 5707
PstI C_TGCA'G
Cuts at: 830 3059 830
Size: 2229 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5707
PvuII CAG'CTG
Cuts at: 2543 2569 2543
Size: 26 5681
SalI G'TCGA_C
Cuts at: 1936 3049 1936
Size: 1113 4594
SapI GCTCTTCn'nnn_
Cuts at: 2230 2230
Size: 5707
SbfI CC_TGCA'GG
Cuts at: 3059 3059
Size: 5707
ScaI AGT'ACT
Cuts at: 2839 3955 5667 2839
Size: 1116 1712 2879
Fragments arranged by size:
2879 1712 1116
SfcI C'TryA_G
Cuts at: 826 1045 2284 2564 3055 4190 4871 5062 826
Size: 219 1239 280 491 1135 681 191 1471
Fragments arranged by size:
1471 1239 1135 681 491 280 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5707
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5707
SmaI CCC'GGG
Cuts at: 1872 3034 1872
Size: 1162 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2080 3811 4712 4974 820
Size: 197 926 137 1731 901 262 1553
Fragments arranged by size:
1731 1553 926 901 262 197 137
SnaBI TAC'GTA
Cuts at: 493 3498 493
Size: 3005 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5707
SphI G_CATG'C
Cuts at: 1132 3065 1132
Size: 1933 3774
SspI AAT'ATT
Cuts at: 5 52 3631 5
Size: 47 3579 2081
Fragments arranged by size:
3579 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5707
StyI C'CwwG_G
Cuts at: 513 1065 2004 3146 513
Size: 552 939 1142 3074
Fragments arranged by size:
3074 1142 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2004 2388 2848 3734 3751 3904 4089
Size: 1255 424 384 460 886 17 153 185
Cuts at: 4089 4534 325
Size: 445 1498
Fragments arranged by size:
1498 1255 886 460 445 424 384 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2837 3953 5665
Size: 274 80 33 51 1443 860 1116 1712
Cuts at: 5665 96
Size: 138
Fragments arranged by size:
1712 1443 1116 860 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5667 5699 5667
Size: 32 5675
Tth111I GACn'n_nGTC
Cuts at: 1316 2340 1316
Size: 1024 4683
XbaI T'CTAG_A
Cuts at: 3043 3043
Size: 5707
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2566 2566
Size: 5707
XhoI C'TCGA_G
Cuts at: 1943 2080 1943
Size: 137 5570
XmaI C'CCGG_G
Cuts at: 1870 3032 1870
Size: 1162 4545
XmnI GAAnn'nnTTC
Cuts at: 2260 3836 2260
Size: 1576 4131
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2012 3515 276
Size: 53 83 186 936 478 1503 2468
Fragments arranged by size:
2468 1503 936 478 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI
PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PsrI
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
SwaI